CA2241331C - Support solide reutilisable, destine a la synthese d'oligonucleotides, son procede de fabrication et son utilisation - Google Patents

Support solide reutilisable, destine a la synthese d'oligonucleotides, son procede de fabrication et son utilisation Download PDF

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Publication number
CA2241331C
CA2241331C CA002241331A CA2241331A CA2241331C CA 2241331 C CA2241331 C CA 2241331C CA 002241331 A CA002241331 A CA 002241331A CA 2241331 A CA2241331 A CA 2241331A CA 2241331 C CA2241331 C CA 2241331C
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CA
Canada
Prior art keywords
group
unsubstituted
substituted
solid support
hydrogen
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CA002241331A
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English (en)
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CA2241331A1 (fr
Inventor
Richard T. Pon
Shuyuan Yu
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University Technologies International Inc
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University Technologies International Inc
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Publication date
Application filed by University Technologies International Inc filed Critical University Technologies International Inc
Publication of CA2241331A1 publication Critical patent/CA2241331A1/fr
Application granted granted Critical
Publication of CA2241331C publication Critical patent/CA2241331C/fr
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/06—Pyrimidine radicals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07B—GENERAL METHODS OF ORGANIC CHEMISTRY; APPARATUS THEREFOR
    • C07B2200/00—Indexing scheme relating to specific properties of organic compounds
    • C07B2200/11—Compounds covalently bound to a solid support

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Saccharide Compounds (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Heterocyclic Carbon Compounds Containing A Hetero Ring Having Oxygen Or Sulfur (AREA)
  • Low-Molecular Organic Synthesis Reactions Using Catalysts (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Handcart (AREA)
  • Forklifts And Lifting Vehicles (AREA)

Abstract

L'invention concerne un support solide pour la synthèse d'oligonucléotides. Le support solide a la formule (1). Dans cette formule, R?8¿ est un groupe C¿1?-C¿20? alkyle substitué ou non, un groupe C¿5?-C¿30? aryle substitué ou non ou un groupe C¿5?-C¿40? alkylaryle substitué ou non; X?3¿ et X?4¿ sont les mêmes ou différents et ce sont -O-, -S-, -S(O)¿2?- ou -(N(R?12¿)-; R?12¿ est un groupe C¿1?-C¿20? alkyle substitué ou non, un groupe C¿5?-C¿30? aryle substitué ou non ou un groupe C¿5?-C¿40? alkylaryle substitué ou non; et Y est -CH¿2?-CH¿2?-; -CH¿2?-; -CH¿2?-O-CH¿2?-; -CH¿2?-CH¿2?-CH¿2?-; -CH=CH-; -CH=C(CH¿3?)-; -C(CH¿3?)=C(CH¿3?)-; -CH¿2?-C(=CH¿2?)-; and -CH¿2?-S-CH¿2?-; où quand Y est -CH¿2?-CH¿2?-, au moins un d'entre X?3¿ et X?4¿ est -O-. L'invention concerne également un bras de liaison fixé sur un support solide, pour la synthèse d'oligonucléotides. L'invention concerne en outre un procédé pour fabriquer le support solide et le bras de liaison. Le bras de liaison est caractérisé par le fait qu'il est réutilisable dans ce procédé de synthèse d'oligonucléotides qui, par ailleurs, est un procédé tout à fait classique.
CA002241331A 1995-12-22 1996-12-13 Support solide reutilisable, destine a la synthese d'oligonucleotides, son procede de fabrication et son utilisation Expired - Fee Related CA2241331C (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US920895P 1995-12-22 1995-12-22
US60/009,208 1995-12-22
PCT/CA1996/000836 WO1997023496A1 (fr) 1995-12-22 1996-12-13 Support solide reutilisable, destine a la synthese d'oligonucleotides, son procede de fabrication et son utilisation

Publications (2)

Publication Number Publication Date
CA2241331A1 CA2241331A1 (fr) 1997-07-03
CA2241331C true CA2241331C (fr) 2003-06-17

Family

ID=21736236

Family Applications (2)

Application Number Title Priority Date Filing Date
CA002241331A Expired - Fee Related CA2241331C (fr) 1995-12-22 1996-12-13 Support solide reutilisable, destine a la synthese d'oligonucleotides, son procede de fabrication et son utilisation
CA002241222A Expired - Fee Related CA2241222C (fr) 1995-12-22 1996-12-13 Bras de liaison pour la synthese d'oligonucleotides sur un support solide et procede pour le former

Family Applications After (1)

Application Number Title Priority Date Filing Date
CA002241222A Expired - Fee Related CA2241222C (fr) 1995-12-22 1996-12-13 Bras de liaison pour la synthese d'oligonucleotides sur un support solide et procede pour le former

Country Status (8)

Country Link
US (1) US6015895A (fr)
EP (2) EP0876390B1 (fr)
JP (2) JP2000502341A (fr)
AT (2) ATE252109T1 (fr)
AU (2) AU725627B2 (fr)
CA (2) CA2241331C (fr)
DE (2) DE69630400T2 (fr)
WO (2) WO1997023497A1 (fr)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6043353A (en) * 1995-12-22 2000-03-28 University Technologies International, Inc. Reusable solid support for oligonucleotide synthesis, process for production thereof and process for use thereof
ID28181A (id) * 1998-02-09 2001-05-10 Toyo Kohan Co Ltd Substrat-substrat untuk menghentikan dan menguatkan dna, dna yang tidak digerakkan oleh chip yang memiliki dna yang tidak bergerak di dalam substrat, metode untuk menguatkan dna
EP1054726B1 (fr) * 1998-02-11 2003-07-30 University of Houston, Office of Technology Transfer Appareil pour induire des reactions biochimiques au moyen de reactifs photogeneres
JP2002519433A (ja) * 1998-07-02 2002-07-02 ユニバーシティー・テクノロジーズ・インターナショナル・インコーポレイテッド オリゴヌクレオチド合成用の再使用可能な固相支持体
US7135564B1 (en) * 1998-07-02 2006-11-14 University Technologies International Inc. Reusable solid support for oligonucleotide synthesis
US6924359B1 (en) * 1999-07-01 2005-08-02 Yale University Neovascular-targeted immunoconjugates
JP3786248B2 (ja) * 1999-11-11 2006-06-14 東洋鋼鈑株式会社 化学修飾を施した基体およびその製造方法
US20030129593A1 (en) * 2000-09-05 2003-07-10 University Technologies International Inc. Process for producing multiple oligonucleotides on a solid support
AU2001291540A1 (en) * 2000-09-08 2002-03-22 University Technologies International, Inc. Linker phosphoramidites for oligonucleotide synthesis
JP2004248503A (ja) * 2000-10-18 2004-09-09 Takara Holdings Inc 核酸固定化用担体
JP2002199881A (ja) 2000-12-28 2002-07-16 Toyo Kohan Co Ltd Ｄｎａ固定化基体の再使用法
US7211654B2 (en) 2001-03-14 2007-05-01 Regents Of The University Of Michigan Linkers and co-coupling agents for optimization of oligonucleotide synthesis and purification on solid supports
US7067622B2 (en) 2001-06-25 2006-06-27 Japan Science And Technology Agency Method of the solid phase synthesis of pyrrole-imidazole polyamide
FR2826957B1 (fr) * 2001-07-09 2005-09-30 Centre Nat Rech Scient Procede de fonctionnalisation de supports solides, supports solides fonctionnalises et leurs utilisations
US20030092062A1 (en) * 2001-10-24 2003-05-15 Reddy M. Parameswara Immobilizing biological molecules
US20030162160A1 (en) * 2002-02-27 2003-08-28 Jack Horchler Interactive puzzle
US7202264B2 (en) 2003-01-31 2007-04-10 Isis Pharmaceuticals, Inc. Supports for oligomer synthesis
EP2708541B1 (fr) * 2003-11-13 2015-01-28 Isis Pharmaceuticals, Inc. Dérivés de 5, 6-dihydroxy-isoindole en tant que lieurs pour une synthèse en phase solide d'oligomères
GB0428020D0 (en) * 2004-12-22 2005-01-26 Amersham Health As Stabilisation of radiopharmaceutical precursors
EP3067359A1 (fr) 2008-09-23 2016-09-14 Scott G. Petersen Pro-oligomères protégés par phosphate biolabiles auto-administrés pour agents thérapeutiques à base d'oligonucléotide et médiation d'arn interférence

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9021625D0 (en) * 1990-10-04 1990-11-21 Ici Plc Synthesis of oligonucleotides
EP1331011A3 (fr) * 1991-10-24 2003-12-17 Isis Pharmaceuticals, Inc. Oligonucléotides dérivés présentant une meilleure facilité d'absorption.
US5476925A (en) * 1993-02-01 1995-12-19 Northwestern University Oligodeoxyribonucleotides including 3'-aminonucleoside-phosphoramidate linkages and terminal 3'-amino groups
DE69420307T2 (de) * 1993-04-05 2000-01-27 Perseptive Biosystems, Inc. Automatisierte Allyl-Deprotektion bei der Festphasesynthese
US5817751A (en) * 1994-06-23 1998-10-06 Affymax Technologies N.V. Method for synthesis of diketopiperazine and diketomorpholine derivatives
US5502068A (en) * 1995-01-31 1996-03-26 Synphar Laboratories, Inc. Cyclopropylpyrroloindole-oligopeptide anticancer agents
US5770687A (en) * 1995-06-07 1998-06-23 Peptor Limited Comformationally constrained backbone cyclized somatostatin analogs
US5798276A (en) * 1995-06-07 1998-08-25 Molecular Probes, Inc. Reactive derivatives of sulforhodamine 101 with enhanced hydrolytic stability
DE19532553A1 (de) * 1995-09-04 1997-03-06 Hoechst Ag Verfahren zur Herstellung substituierter N-Ethyl-Glycinderivate
US5736626A (en) * 1996-01-29 1998-04-07 The Perkin-Elmer Corporation Solid support reagents for the direct synthesis of 3'-labeled polynucleotides

Also Published As

Publication number Publication date
CA2241331A1 (fr) 1997-07-03
CA2241222A1 (fr) 1997-07-03
DE69621836T2 (de) 2003-02-20
WO1997023496A1 (fr) 1997-07-03
EP0876390A1 (fr) 1998-11-11
DE69630400T2 (de) 2005-04-14
EP0877751B1 (fr) 2003-10-15
EP0877751A1 (fr) 1998-11-18
WO1997023497A1 (fr) 1997-07-03
AU1027897A (en) 1997-07-17
US6015895A (en) 2000-01-18
ATE252109T1 (de) 2003-11-15
DE69630400D1 (de) 2003-11-20
DE69621836D1 (de) 2002-07-18
JP2000502341A (ja) 2000-02-29
JP2000502342A (ja) 2000-02-29
CA2241222C (fr) 2003-07-22
ATE219098T1 (de) 2002-06-15
EP0876390B1 (fr) 2002-06-12
AU725627B2 (en) 2000-10-19
AU725690B2 (en) 2000-10-19
AU1027797A (en) 1997-07-17

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