CA2235509A1 - Croissance in vitro d'ilots de langerhans fonctionnels et utilisations in vivo de ces ilots - Google Patents

Croissance in vitro d'ilots de langerhans fonctionnels et utilisations in vivo de ces ilots Download PDF

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Publication number
CA2235509A1
CA2235509A1 CA002235509A CA2235509A CA2235509A1 CA 2235509 A1 CA2235509 A1 CA 2235509A1 CA 002235509 A CA002235509 A CA 002235509A CA 2235509 A CA2235509 A CA 2235509A CA 2235509 A1 CA2235509 A1 CA 2235509A1
Authority
CA
Canada
Prior art keywords
cells
islet
cell
pancreatic
culture
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002235509A
Other languages
English (en)
Inventor
Ammon B. Peck
Janet G. Cornelius
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Florida Research Foundation Inc
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US08/547,746 external-priority patent/US6001647A/en
Application filed by Individual filed Critical Individual
Publication of CA2235509A1 publication Critical patent/CA2235509A1/fr
Abandoned legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0676—Pancreatic cells
    • C12N5/0678—Stem cells; Progenitor cells; Precursor cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
    • A61P3/10—Drugs for disorders of the metabolism for glucose homeostasis for hyperglycaemia, e.g. antidiabetics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/34—Sugars

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Diabetes (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Hematology (AREA)
  • Medicinal Chemistry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Obesity (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • General Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Emergency Medicine (AREA)
  • Endocrinology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

La présente invention se rapporte à de nouveaux procédés qui, pour la première fois, permettent de développer des îlots fonctionnels dans des cultures in vitro. La présente invention se rapporte également à l'utilisation de ces structures de type îlots cultivées in vitro en vue de l'implantation chez un mammifère dans le cadre d'une thérapie in vivo du diabète. La présente invention se rapporte en outre à un procédé utilisant ces implants d'îlots cultivés in vitro pour développer un organe in vivo qui possède les mêmes caractéristiques fonctionnelles, morphologiques et histologiques que celles observées dans le tissu pancréatique normal. La possibilité de faire se développer ces cellules in vitro ainsi que des organes in vivo ouvre d'importantes perspectives de recherche et de thérapie liées aux diabètes.
CA002235509A 1995-10-25 1996-10-23 Croissance in vitro d'ilots de langerhans fonctionnels et utilisations in vivo de ces ilots Abandoned CA2235509A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/547,746 1995-10-25
US08/547,746 US6001647A (en) 1994-04-28 1995-10-25 In vitro growth of functional islets of Langerhans and in vivo uses thereof

Publications (1)

Publication Number Publication Date
CA2235509A1 true CA2235509A1 (fr) 1997-05-01

Family

ID=24185961

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002235509A Abandoned CA2235509A1 (fr) 1995-10-25 1996-10-23 Croissance in vitro d'ilots de langerhans fonctionnels et utilisations in vivo de ces ilots

Country Status (5)

Country Link
EP (1) EP0871455A4 (fr)
JP (1) JPH11514877A (fr)
AU (1) AU739771B2 (fr)
CA (1) CA2235509A1 (fr)
WO (1) WO1997015310A1 (fr)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2359821A1 (fr) * 1999-02-04 2000-08-10 Mcgill University Plate-forme de differenciation cellulaire
GB9914890D0 (en) * 1999-06-25 1999-08-25 Univ Buckingham Methods and compositions relating to pancreatic islet and -cell dysfunction
EP1224259A4 (fr) * 1999-09-27 2005-04-27 Univ Florida Inversion de diabetes dependant de l'insuline par des cellules souches insulaires, des cellules insulaires progenitrices et des structures de type insulaire
WO2001032839A2 (fr) * 1999-10-29 2001-05-10 Mcgill University Milieu servant a la preparation de cellules dedifferenciees
US20030082155A1 (en) 1999-12-06 2003-05-01 Habener Joel F. Stem cells of the islets of langerhans and their use in treating diabetes mellitus
US6866843B2 (en) 1999-12-06 2005-03-15 Viacell, Inc. Method of transplanting in a mammal and treating diabetes mellitus by administering a pseudo-islet like aggregate differentiated from a nestin-positive pancreatic stem cell
AU778155B2 (en) * 1999-12-13 2004-11-18 Scripps Research Institute, The Markers for identification and isolation of pancreatic islet alpha and beta cell progenitors
US6610535B1 (en) * 2000-02-10 2003-08-26 Es Cell International Pte Ltd. Progenitor cells and methods and uses related thereto
US6436704B1 (en) * 2000-04-10 2002-08-20 Raven Biotechnologies, Inc. Human pancreatic epithelial progenitor cells and methods of isolation and use thereof
AU2001273108A1 (en) * 2000-06-30 2002-01-14 Amcyte, Inc. Culturing pancreatic stem cells having a specified, intermediate stage of development
US7202080B2 (en) 2001-03-29 2007-04-10 Ixion Biotechnology, Inc. Method for transdifferentiation of non-pancreatic stem cells to the pancreatic differentiation pathway
WO2002088335A1 (fr) * 2001-04-24 2002-11-07 Ajinomoto Co., Inc. Cellules souches et procede d'extraction de ces cellules
US7101546B2 (en) 2001-12-21 2006-09-05 Amcyte, Inc. In situ maturation of cultured pancreatic stem cells having a specified, intermediate stage of development
EP1539928A4 (fr) 2002-09-06 2006-09-06 Amcyte Inc Cellules progenitrices endocrines pancreatiques positives cd56 chez l'etre humain adulte
EP1629089A1 (fr) * 2003-06-03 2006-03-01 Organ Recovery Systems, Inc. Selection et propagation de cellules progenitrices
JP2007520194A (ja) * 2003-06-20 2007-07-26 ブレイエ・ユニバージテイト・ブリュッセル 外分泌膵臓細胞から、膵島ベータ−細胞を生成する方法
US20060121512A1 (en) * 2004-12-02 2006-06-08 Organ Recovery Systems, Inc. Biological pathways in progenitor cells
WO2007039986A1 (fr) 2005-10-05 2007-04-12 Osaka University Procédé d'obtention de cellules endocrines pancréatiques à partir de cellules provenant d'un tissu adipeux
KR101452877B1 (ko) * 2013-08-05 2014-10-22 사회복지법인 삼성생명공익재단 조기-내피전구세포 및 췌도세포를 포함하는 세포이식물의 제조방법
JP7081483B2 (ja) * 2016-04-19 2022-06-07 凸版印刷株式会社 被検化合物の脈管新生阻害活性の評価方法

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK0605428T5 (da) * 1991-06-24 2003-01-06 Hcell Technology Inc Hormonsecernerende pancreasceller opretholdt i langtidskultur
JP2813467B2 (ja) * 1993-04-08 1998-10-22 ヒューマン・セル・カルチャーズ・インコーポレーテッド 細胞培養法および培地
US5834308A (en) * 1994-04-28 1998-11-10 University Of Florida Research Foundation, Inc. In vitro growth of functional islets of Langerhans

Also Published As

Publication number Publication date
MX9803263A (es) 1998-09-30
AU739771B2 (en) 2001-10-18
EP0871455A4 (fr) 2003-05-14
JPH11514877A (ja) 1999-12-21
AU7468396A (en) 1997-05-15
WO1997015310A1 (fr) 1997-05-01
EP0871455A1 (fr) 1998-10-21

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Legal Events

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EEER Examination request
FZDE Dead