CA2223492A1 - Isolation de cellules souches hematopo?etiques de mammaliennes - Google Patents

Isolation de cellules souches hematopo?etiques de mammaliennes Download PDF

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Publication number
CA2223492A1
CA2223492A1 CA002223492A CA2223492A CA2223492A1 CA 2223492 A1 CA2223492 A1 CA 2223492A1 CA 002223492 A CA002223492 A CA 002223492A CA 2223492 A CA2223492 A CA 2223492A CA 2223492 A1 CA2223492 A1 CA 2223492A1
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CA
Canada
Prior art keywords
cells
dye
hematopoietic stem
stem cells
purified
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002223492A
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English (en)
Inventor
Margaret A. Goodell
A. Stewart Conner
Richard C. Mulligan
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Whitehead Institute for Biomedical Research
Massachusetts Institute of Technology
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Whitehead Institute for Biomedical Research, Massachusetts Institute of Technology filed Critical Whitehead Institute for Biomedical Research
Publication of CA2223492A1 publication Critical patent/CA2223492A1/fr
Abandoned legal-status Critical Current

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Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/28—Bone marrow; Haematopoietic stem cells; Mesenchymal stem cells of any origin, e.g. adipose-derived stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0647—Haematopoietic stem cells; Uncommitted or multipotent progenitors
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Biotechnology (AREA)
  • Cell Biology (AREA)
  • Zoology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Virology (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Un procédé permet de purifier des cellules souches hématopoïétiques mammaliennes provenant d'une population de cellules de moëlle osseuse. Un autre procédé permet de séparer des cellules souches hématopoïétiques actives de celles qui sont quiescentes dans un échantillon de ces cellules purifiées mammaliennes. On décrit aussi un procédé d'identification d'un agent qui est un colorant vital fluorescent et lipophile qu'on utilise pour purifier ces cellules mammaliennes, ainsi qu'un procédé de greffe de moëlle osseuse chez un mammifère, tel qu'un être humain, procédé qui consiste à introduire chez l'hôte les cellules souches hématopoïétiques purifiées décrites. On décrit en outre un procédé d'administration in vivo d'une protéine, qui consiste à transfecter une cellule souche hématopoïétique purifiée avec un produit d'assemblage comprenant de l'ADN qui code une protéine souhaitée, puis à introduire cette cellule souche chez l'hôte où cette protéine s'exprime. On décrit aussi des cellules souches hématopoïétiques purifiées à l'aide des procédés décrits.
CA002223492A 1995-06-06 1996-06-05 Isolation de cellules souches hematopo?etiques de mammaliennes Abandoned CA2223492A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US47175895A 1995-06-06 1995-06-06
US08/471,758 1995-06-06

Publications (1)

Publication Number Publication Date
CA2223492A1 true CA2223492A1 (fr) 1996-12-12

Family

ID=23872883

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002223492A Abandoned CA2223492A1 (fr) 1995-06-06 1996-06-05 Isolation de cellules souches hematopo?etiques de mammaliennes

Country Status (3)

Country Link
EP (1) EP0833893A1 (fr)
CA (1) CA2223492A1 (fr)
WO (1) WO1996039489A1 (fr)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2219869A1 (fr) * 1997-10-31 1999-04-30 Hsc Research And Development Limited Partnership Cellule de souche humaine cd-34 hematopoietique
CA2313250A1 (fr) * 1997-12-04 1999-06-10 Duke University Procedes d'isolation et d'utilisation des cellules hematopoietiques cd7+cd34-lin-
CA2386536A1 (fr) * 1999-09-14 2001-03-22 The Children's Medical Center Corporation Methodes de traitement des dystrophies musculaires
DE60221987T2 (de) 2001-06-22 2008-05-15 Stemcells, Inc., Palo Alto Le-zellen (liver engrafting cells), assays und verwendungen davon
JP2007516230A (ja) 2003-05-19 2007-06-21 トラスティーズ・オブ・コロンビア・ユニバーシティ・イン・ザ・シティ・オブ・ニューヨーク 心臓組織の変性を治療及び予防するための組成物及び方法並びにその用途
WO2014072960A2 (fr) * 2012-11-12 2014-05-15 National Centre For Biological Sciences Procédé pour identifier et isoler des cellules souches pluripotentes à l'aide de la fluorescence bleue endogène
CN107072716A (zh) 2014-05-30 2017-08-18 托德.F.奥沃凯蒂斯 用于产生和使用活化的干细胞的方法和系统
US10202598B2 (en) 2014-05-30 2019-02-12 Todd Frank Ovokaitys Methods and systems for generation, use, and delivery of activated stem cells
US10040728B2 (en) 2014-06-06 2018-08-07 Todd Frank Ovokaitys Methods and compositions for increasing the bioactivity of nutrients
US10384985B2 (en) 2014-06-06 2019-08-20 B.K. Consultants, Inc. Methods and compositions for increasing the yield of, and beneficial chemical composition of, certain plants

Also Published As

Publication number Publication date
EP0833893A1 (fr) 1998-04-08
WO1996039489A1 (fr) 1996-12-12

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