CA2202990A1 - Use of spermidine to relieve inhibition of ligase chain reaction in a clinical test sample - Google Patents

Use of spermidine to relieve inhibition of ligase chain reaction in a clinical test sample

Info

Publication number
CA2202990A1
CA2202990A1 CA 2202990 CA2202990A CA2202990A1 CA 2202990 A1 CA2202990 A1 CA 2202990A1 CA 2202990 CA2202990 CA 2202990 CA 2202990 A CA2202990 A CA 2202990A CA 2202990 A1 CA2202990 A1 CA 2202990A1
Authority
CA
Canada
Prior art keywords
target
spermidine
probes
probe
amplification
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA 2202990
Other languages
English (en)
French (fr)
Inventor
Alan H. Davis
Jianli Cao
Eui H. Lee
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Abbott Laboratories
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2202990A1 publication Critical patent/CA2202990A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6862—Ligase chain reaction [LCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CA 2202990 1994-10-21 1995-10-18 Use of spermidine to relieve inhibition of ligase chain reaction in a clinical test sample Abandoned CA2202990A1 (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US33139194A 1994-10-21 1994-10-21
US08/331,391 1994-10-21
US53221295A 1995-10-12 1995-10-12
US08/532,212 1995-10-12

Publications (1)

Publication Number Publication Date
CA2202990A1 true CA2202990A1 (en) 1996-05-02

Family

ID=26987744

Family Applications (1)

Application Number Title Priority Date Filing Date
CA 2202990 Abandoned CA2202990A1 (en) 1994-10-21 1995-10-18 Use of spermidine to relieve inhibition of ligase chain reaction in a clinical test sample

Country Status (3)

Country Link
EP (1) EP0791075A2 (de)
CA (1) CA2202990A1 (de)
WO (1) WO1996012824A2 (de)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6136533A (en) * 1997-07-03 2000-10-24 Id Biomedical Additives for use in cycling probe reactions
AU4754300A (en) * 1999-04-30 2000-12-12 Connex Gesellschaft Zur Optimierung Von Forschung Und Entwicklung Mbh Mixture for neutralizing enzyme inhibitors
US20150218608A1 (en) * 2014-01-31 2015-08-06 New England Biolabs, Inc. Compositions and Methods for Improvement of Ligation Yields
US10900092B2 (en) 2016-03-30 2021-01-26 Fujifilm Wako Pure Chemical Corporation Chlamydia trachomatis detecting primer set, Chlamydia trachomatis detecting method using same, and reagent kit therefor

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6236187A (ja) * 1985-08-09 1987-02-17 Takara Shuzo Co Ltd Ｄｎａリガ−ゼ組成物及びその使用方法
CA1323293C (en) * 1987-12-11 1993-10-19 Keith C. Backman Assay using template-dependent nucleic acid probe reorganization
DE69133582T2 (de) * 1990-05-03 2008-07-24 Cornell Research Foundation, Inc. Ein system der dna amplifikation zur detektion genetischer erkrankungen durch eine thermostabile ligase
JP3416981B2 (ja) * 1993-03-30 2003-06-16 株式会社島津製作所 核酸合成法

Also Published As

Publication number Publication date
EP0791075A2 (de) 1997-08-27
WO1996012824A2 (en) 1996-05-02
WO1996012824A3 (en) 1996-06-27

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Legal Events

Date Code Title Description
FZDE Dead