CA2200294A1 - Enrichissement de cellules foetales, a partir du sang maternel - Google Patents

Enrichissement de cellules foetales, a partir du sang maternel

Info

Publication number
CA2200294A1
CA2200294A1 CA002200294A CA2200294A CA2200294A1 CA 2200294 A1 CA2200294 A1 CA 2200294A1 CA 002200294 A CA002200294 A CA 002200294A CA 2200294 A CA2200294 A CA 2200294A CA 2200294 A1 CA2200294 A1 CA 2200294A1
Authority
CA
Canada
Prior art keywords
cells
fetal
suspension
dna
matrix
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002200294A
Other languages
English (en)
Inventor
Jurgen Busch
Andreas Radbruch
Stefan Miltenyi
Jurgen Holtz
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2200294A1 publication Critical patent/CA2200294A1/fr
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804—Nucleic acid analysis using immunogens
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G01N33/54326—Magnetic particles
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56966—Animal cells
    • G01N33/56972—White blood cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/689—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids related to pregnancy or the gonads
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2800/00—Detection or diagnosis of diseases
    • G01N2800/36—Gynecology or obstetrics
    • G01N2800/368—Pregnancy complicated by disease or abnormalities of pregnancy, e.g. preeclampsia, preterm labour

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Hematology (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Pathology (AREA)
  • Biochemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Organic Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Gynecology & Obstetrics (AREA)
  • Pregnancy & Childbirth (AREA)
  • Reproductive Health (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Procédés pour préparer des cellules foetales à partir d'échantillons de sang périphérique maternel. Une suspension de cellules mononucléées est préparée à partir d'un échantillon de sang maternel. Les cellules maternelles sont ensuite appauvries à partir de l'échantillon par l'addition d'anticorps couplés avec des particules magnétiques et spécifiques des marqueurs présents sur les cellules adultes, et la fixation des cellules dans une colonne en présence d'un champ magnétique. A partir de la fraction appauvrie les érythrocytes du foetus sont enrichis par triage magnétique des cellules. Ces érythrocytes nucléés sont alors utilisés comme source de matériau génétique du foetus, qui est analysé pour mettre en évidence des anomalies chromosomiques ou génétiques, ou pour rechercher la présence d'un chromosome Y, par des procédés conventionnels tels que l'hybridation in situ avec des sondes, l'examen des dispersions en métaphase et la technique d'amplification en chaîne PCR.
CA002200294A 1994-09-20 1995-09-19 Enrichissement de cellules foetales, a partir du sang maternel Abandoned CA2200294A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US30922994A 1994-09-20 1994-09-20
US08/309,229 1994-09-20

Publications (1)

Publication Number Publication Date
CA2200294A1 true CA2200294A1 (fr) 1996-03-28

Family

ID=23197274

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002200294A Abandoned CA2200294A1 (fr) 1994-09-20 1995-09-19 Enrichissement de cellules foetales, a partir du sang maternel

Country Status (5)

Country Link
EP (1) EP0778899A4 (fr)
JP (1) JPH10507632A (fr)
AU (1) AU691040B2 (fr)
CA (1) CA2200294A1 (fr)
WO (1) WO1996009409A1 (fr)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IT1294964B1 (it) * 1996-07-12 1999-04-23 Domenico Valerio Isolamento e cultura di cellule fetali dal sangue periferico materno
AU4113297A (en) 1996-09-04 1998-03-26 Technical University Of Denmark A micro flow system for particle separation and analysis
FR2758884B1 (fr) * 1997-01-30 1999-04-02 Bio Merieux Procede pour isoler, notamment detecter ou quantifier un analyte dans un milieu
CA2283416A1 (fr) * 1997-03-08 1998-09-17 The University Of Dundee Procedes de diagnostic prenatal
GB0009179D0 (en) * 2000-04-13 2000-05-31 Imp College Innovations Ltd Non-invasive prenatal diagnosis
GB0616045D0 (en) * 2006-08-11 2006-09-20 Univ Bristol Blood cell separation
GB2447255A (en) * 2007-03-02 2008-09-10 Oncoprobe Ltd Preparation of enriched target cell samples for use in a chemosensitivity assay
FR2963108B1 (fr) * 2010-07-21 2017-06-23 Diagast Procede magnetique d'immunodiagnostic et kit pour la mise en evidence de complexe anticorps/antigene de groupe/phenotype sanguin
ES2699743T3 (es) * 2011-12-17 2019-02-12 Bgi Genomics Co Ltd Procedimiento y sistema para determinar si un genoma es anormal
EP2955521A1 (fr) * 2014-06-11 2015-12-16 Centre Hospitalier Universitaire Vaudois (CHUV) Procédés pour séparer des cellules
CN109718880A (zh) * 2019-02-20 2019-05-07 广州睿辰生物科技有限公司 基于双抗法分选胎儿有核红细胞额定微流控芯片及其分选方法

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4230685A (en) * 1979-02-28 1980-10-28 Northwestern University Method of magnetic separation of cells and the like, and microspheres for use therein
US4452773A (en) * 1982-04-05 1984-06-05 Canadian Patents And Development Limited Magnetic iron-dextran microspheres
JPH05507404A (ja) * 1990-04-23 1993-10-28 セルプロ　インコーポレイティド 母体血液からの胎児細胞の富化のための方法

Also Published As

Publication number Publication date
WO1996009409A1 (fr) 1996-03-28
EP0778899A4 (fr) 2001-08-29
JPH10507632A (ja) 1998-07-28
AU691040B2 (en) 1998-05-07
EP0778899A1 (fr) 1997-06-18
AU3593595A (en) 1996-04-09

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Legal Events

Date Code Title Description
FZDE Dead