CA2192324C - Virus de recombinaison et methode pour le quantifier - Google Patents

Virus de recombinaison et methode pour le quantifier Download PDF

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Publication number
CA2192324C
CA2192324C CA002192324A CA2192324A CA2192324C CA 2192324 C CA2192324 C CA 2192324C CA 002192324 A CA002192324 A CA 002192324A CA 2192324 A CA2192324 A CA 2192324A CA 2192324 C CA2192324 C CA 2192324C
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CA
Canada
Prior art keywords
nucleic acid
virus
wild type
tagged
sample
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CA002192324A
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English (en)
Other versions
CA2192324A1 (fr
Inventor
Venkatachala Natarajan
Norman P. Salzman
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Georgetown University
Original Assignee
Georgetown University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Georgetown University filed Critical Georgetown University
Publication of CA2192324A1 publication Critical patent/CA2192324A1/fr
Application granted granted Critical
Publication of CA2192324C publication Critical patent/CA2192324C/fr
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/702—Specific hybridization probes for retroviruses
    • C12Q1/703—Viruses associated with AIDS
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/16011—Human Immunodeficiency Virus, HIV
    • C12N2740/16211—Human Immunodeficiency Virus, HIV concerning HIV gagpol
    • C12N2740/16222—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Virology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • General Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • AIDS & HIV (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

L'invention concerne une méthode de quantification de virus et d'acides nucléiques viraux. L'invention concerne des méthodes pour quantifier la quantité de virus présente dans un échantillon, consistant à introduire dans l'échantillon une composition comprenant un acide nucléique viral marqué génétiquement, isoler l'acide nucléique sauvage et ledit acide nucléique marqué et doser l'acide nucléique sauvage et l'acide nucléique marqué. L'invention concerne également des acides nucléiques de rétrovirus marqués génétiquement comprenant une séquence de marquage avec des insertions et des délétions.
CA002192324A 1994-06-10 1994-06-10 Virus de recombinaison et methode pour le quantifier Expired - Fee Related CA2192324C (fr)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/US1994/006631 WO1995034684A1 (fr) 1994-06-10 1994-06-10 Virus de recombinaison et methode pour le quantifier

Publications (2)

Publication Number Publication Date
CA2192324A1 CA2192324A1 (fr) 1995-12-21
CA2192324C true CA2192324C (fr) 2006-10-10

Family

ID=22242665

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002192324A Expired - Fee Related CA2192324C (fr) 1994-06-10 1994-06-10 Virus de recombinaison et methode pour le quantifier

Country Status (4)

Country Link
JP (1) JPH10505224A (fr)
AU (1) AU692792B2 (fr)
CA (1) CA2192324C (fr)
WO (1) WO1995034684A1 (fr)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5939262A (en) * 1996-07-03 1999-08-17 Ambion, Inc. Ribonuclease resistant RNA preparation and utilization
US5677124A (en) * 1996-07-03 1997-10-14 Ambion, Inc. Ribonuclease resistant viral RNA standards
US5989499A (en) * 1997-05-02 1999-11-23 Biomerieux, Inc. Dual chamber disposable reaction vessel for amplification reactions
US6429007B1 (en) 1997-05-02 2002-08-06 BIOMéRIEUX, INC. Nucleic acid amplification reaction station for disposable test devices
US6410275B1 (en) 1997-05-02 2002-06-25 Biomerieux, Inc. Disposable test devices for performing nucleic acid amplification reactions
DE19737442C2 (de) * 1997-08-22 1999-06-24 Olfert Landt Tib Molbiol Synth Proteinumhüllte Polyribonukleinsäuren, ein Verfahren zu ihrer Herstellung und ihre Verwendung
WO1999013113A1 (fr) * 1997-09-12 1999-03-18 The Public Health Research Institute Of The City Of New York, Inc. Procedes de co-amplification non competitive
US8012719B2 (en) 1998-11-17 2011-09-06 Paolo Lusso Method for the quantitative detection of nucleic acids
IT1303767B1 (it) 1998-11-17 2001-02-23 San Raffaele Centro Fond Metodo di quantificazione di acidi nucleici.
WO2000063440A2 (fr) * 1999-04-20 2000-10-26 The Government Of The United States Of America, As Represented By The Secretary, Department Of Health & Human Services Nouveaux standards diagnostiques de detection et quantification de virus
BRPI0600715B8 (pt) * 2006-03-16 2021-07-27 Fundacao Oswaldo Cruz vírus calibrador artificial (vca), método para garantir a detecção e/ou quantificação da carga viral, kit para detecção viral e processo de obtenção de vírus calibrador artificial
US20110053145A1 (en) 2008-03-31 2011-03-03 Japan Tobacco Inc. Quantitation method of virus
GB201002434D0 (en) 2010-02-12 2010-03-31 Plant Bioscience Ltd Non-infectious RNA source

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5256545A (en) * 1987-03-30 1993-10-26 Board Of Regents, The University Of Texas System Sterol Regulatory Elements
US5278056A (en) * 1988-02-05 1994-01-11 The Trustees Of Columbia University In The City Of New York Retroviral packaging cell lines and process of using same
US5180666A (en) * 1991-06-27 1993-01-19 Wayne State University Method and cell line for testing mutagenicity of a chemical
US5262311A (en) * 1992-03-11 1993-11-16 Dana-Farber Cancer Institute, Inc. Methods to clone polyA mRNA

Also Published As

Publication number Publication date
JPH10505224A (ja) 1998-05-26
WO1995034684A1 (fr) 1995-12-21
CA2192324A1 (fr) 1995-12-21
AU7512394A (en) 1996-01-05
AU692792B2 (en) 1998-06-18

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Effective date: 20140610