CA2188257A1 - Adn codant les enzymes de fin de biosynthese des cephamycines - Google Patents

Adn codant les enzymes de fin de biosynthese des cephamycines

Info

Publication number
CA2188257A1
CA2188257A1 CA002188257A CA2188257A CA2188257A1 CA 2188257 A1 CA2188257 A1 CA 2188257A1 CA 002188257 A CA002188257 A CA 002188257A CA 2188257 A CA2188257 A CA 2188257A CA 2188257 A1 CA2188257 A1 CA 2188257A1
Authority
CA
Canada
Prior art keywords
protein
dna
leu
ala
arg
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002188257A
Other languages
English (en)
Inventor
Juan Jose R. Coque
Francisco J. Enguita
Juan L. Fuente
Francisco J. Llarena
Paloma Liras
Juan F. Martin
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Merck and Co Inc
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2188257A1 publication Critical patent/CA2188257A1/fr
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1003—Transferases (2.) transferring one-carbon groups (2.1)
    • C12N9/1018—Carboxy- and carbamoyl transferases (2.1.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P35/00—Preparation of compounds having a 5-thia-1-azabicyclo [4.2.0] octane ring system, e.g. cephalosporin
    • C12P35/06—Cephalosporin C; Derivatives thereof

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Les ADN codant les enzymes tardifs participant à la synthèse des antibiotiques de la famille des céphamycines ont été isolés et purifiés. Ces enzymes particuliers participent aux dernières phases de la biosynthèse des céphamycines. Ces ADN ont été séquencés et clonés sous forme de vecteurs d'expression de recombinaison permettant leur expression de recombinaison dans des cellules hôtes. Les ADN, les vecteurs les contenant et les cellules hôtes de recombinaison les exprimant sont utiles pour la production d'antibiotiques.
CA002188257A 1994-04-22 1995-04-17 Adn codant les enzymes de fin de biosynthese des cephamycines Abandoned CA2188257A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US23360594A 1994-04-22 1994-04-22
US233,605 1994-04-22

Publications (1)

Publication Number Publication Date
CA2188257A1 true CA2188257A1 (fr) 1995-11-02

Family

ID=22877948

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002188257A Abandoned CA2188257A1 (fr) 1994-04-22 1995-04-17 Adn codant les enzymes de fin de biosynthese des cephamycines

Country Status (5)

Country Link
EP (1) EP0763129A1 (fr)
JP (1) JPH09512170A (fr)
AU (1) AU2358095A (fr)
CA (1) CA2188257A1 (fr)
WO (1) WO1995029253A1 (fr)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4902925B2 (ja) 2000-02-08 2012-03-21 シンヴェント エイエス ナイスタチンポリケチドシンターゼをコードする新規の遺伝子、およびそれらの取扱ならびに用途
EP1658296B1 (fr) 2003-05-28 2012-12-19 DSM Sinochem Pharmaceuticals Netherlands B.V. Composes cephem
WO2009091790A1 (fr) 2008-01-15 2009-07-23 Invista Technologies S.A.R.L. Hydrocyanation de pentènenitriles

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU1780542C (ru) * 1985-04-22 1992-12-07 Эли Лилли Энд Компани Способ конструировани рекомбинантной плазмидной ДНК pPS 20, кодирующей изопенициллин-N-синтетазу, способ получени штамма СернаLоSроRIUм асRемоNIUм, обладающего активностью изопенициллин-N-синтетазы
US5070020A (en) * 1988-05-09 1991-12-03 Eli Lilly And Company Recombinant dna expression vectors and dna compounds that encode deacetoxycephalosporin c synthetase

Also Published As

Publication number Publication date
JPH09512170A (ja) 1997-12-09
WO1995029253A1 (fr) 1995-11-02
EP0763129A1 (fr) 1997-03-19
AU2358095A (en) 1995-11-16

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Legal Events

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