CA2173793C - Pentose fermentation by recombinant zymomonas - Google Patents
Pentose fermentation by recombinant zymomonas Download PDFInfo
- Publication number
- CA2173793C CA2173793C CA2173793A CA2173793A CA2173793C CA 2173793 C CA2173793 C CA 2173793C CA 2173793 A CA2173793 A CA 2173793A CA 2173793 A CA2173793 A CA 2173793A CA 2173793 C CA2173793 C CA 2173793C
- Authority
- CA
- Canada
- Prior art keywords
- microorganism
- genes
- transaldolase
- transketolase
- arabinose
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 238000000855 fermentation Methods 0.000 title claims abstract description 56
- 230000004151 fermentation Effects 0.000 title claims abstract description 56
- 150000002972 pentoses Chemical class 0.000 title claims abstract description 46
- 241000588901 Zymomonas Species 0.000 title claims description 68
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 248
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 134
- 244000005700 microbiome Species 0.000 claims abstract description 79
- 235000000346 sugar Nutrition 0.000 claims abstract description 66
- 108020004530 Transaldolase Proteins 0.000 claims abstract description 58
- 108010043652 Transketolase Proteins 0.000 claims abstract description 55
- 102100028601 Transaldolase Human genes 0.000 claims abstract description 50
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims abstract description 48
- 241000588902 Zymomonas mobilis Species 0.000 claims abstract description 43
- 239000001913 cellulose Substances 0.000 claims abstract description 40
- 229920002678 cellulose Polymers 0.000 claims abstract description 40
- 239000008103 glucose Substances 0.000 claims abstract description 38
- 108700040099 Xylose isomerases Proteins 0.000 claims abstract description 31
- 102000014701 Transketolase Human genes 0.000 claims abstract description 30
- 108010018080 L-arabinose isomerase Proteins 0.000 claims abstract description 26
- 108020002667 ribulokinase Proteins 0.000 claims abstract description 26
- 241000588724 Escherichia coli Species 0.000 claims abstract description 25
- 108090000416 L-ribulose-5-phosphate 4-epimerases Proteins 0.000 claims abstract description 25
- 108091022915 xylulokinase Proteins 0.000 claims abstract description 23
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 claims abstract description 20
- 102100029089 Xylulose kinase Human genes 0.000 claims abstract description 15
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 claims description 156
- SRBFZHDQGSBBOR-IOVATXLUSA-N Xylose Natural products O[C@@H]1COC(O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-IOVATXLUSA-N 0.000 claims description 146
- PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 claims description 134
- 239000013612 plasmid Substances 0.000 claims description 77
- PYMYPHUHKUWMLA-WDCZJNDASA-N arabinose Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)C=O PYMYPHUHKUWMLA-WDCZJNDASA-N 0.000 claims description 62
- 102000006602 glyceraldehyde-3-phosphate dehydrogenase Human genes 0.000 claims description 38
- 108020004445 glyceraldehyde-3-phosphate dehydrogenase Proteins 0.000 claims description 38
- 238000000034 method Methods 0.000 claims description 36
- 230000008569 process Effects 0.000 claims description 27
- 102000004190 Enzymes Human genes 0.000 claims description 24
- 108090000790 Enzymes Proteins 0.000 claims description 24
- 229940088598 enzyme Drugs 0.000 claims description 24
- 102000012288 Phosphopyruvate Hydratase Human genes 0.000 claims description 23
- 108010022181 Phosphopyruvate Hydratase Proteins 0.000 claims description 23
- 239000013598 vector Substances 0.000 claims description 21
- 108010059892 Cellulase Proteins 0.000 claims description 16
- 230000003301 hydrolyzing effect Effects 0.000 claims 3
- 125000000089 arabinosyl group Chemical group C1([C@@H](O)[C@H](O)[C@H](O)CO1)* 0.000 claims 2
- 125000000969 xylosyl group Chemical group C1([C@H](O)[C@@H](O)[C@H](O)CO1)* 0.000 claims 1
- 229920002488 Hemicellulose Polymers 0.000 abstract description 8
- 230000007062 hydrolysis Effects 0.000 abstract 1
- 238000006460 hydrolysis reaction Methods 0.000 abstract 1
- 108020004414 DNA Proteins 0.000 description 89
- 239000012634 fragment Substances 0.000 description 62
- 235000010980 cellulose Nutrition 0.000 description 36
- LXJXRIRHZLFYRP-UHFFFAOYSA-N glyceraldehyde 3-phosphate Chemical compound O=CC(O)COP(O)(O)=O LXJXRIRHZLFYRP-UHFFFAOYSA-N 0.000 description 31
- 239000000203 mixture Substances 0.000 description 26
- 210000004027 cell Anatomy 0.000 description 25
- 108091028043 Nucleic acid sequence Proteins 0.000 description 24
- 238000003752 polymerase chain reaction Methods 0.000 description 23
- 108091008146 restriction endonucleases Proteins 0.000 description 23
- 239000002609 medium Substances 0.000 description 20
- 108020004707 nucleic acids Proteins 0.000 description 19
- 102000039446 nucleic acids Human genes 0.000 description 19
- 150000007523 nucleic acids Chemical class 0.000 description 19
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 description 18
- 230000000692 anti-sense effect Effects 0.000 description 18
- 239000002585 base Substances 0.000 description 18
- -1 pentose sugars Chemical class 0.000 description 18
- 238000004458 analytical method Methods 0.000 description 17
- 239000000758 substrate Substances 0.000 description 17
- 150000008163 sugars Chemical class 0.000 description 17
- 230000037353 metabolic pathway Effects 0.000 description 15
- 230000004108 pentose phosphate pathway Effects 0.000 description 15
- 239000004098 Tetracycline Substances 0.000 description 14
- 230000004927 fusion Effects 0.000 description 14
- 229960002180 tetracycline Drugs 0.000 description 14
- 229930101283 tetracycline Natural products 0.000 description 14
- 235000019364 tetracycline Nutrition 0.000 description 14
- 150000003522 tetracyclines Chemical class 0.000 description 14
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 13
- 229910052799 carbon Inorganic materials 0.000 description 13
- 101150097746 araB gene Proteins 0.000 description 12
- 230000015572 biosynthetic process Effects 0.000 description 12
- 230000012010 growth Effects 0.000 description 12
- 238000003786 synthesis reaction Methods 0.000 description 12
- SRBFZHDQGSBBOR-HWQSCIPKSA-N L-arabinopyranose Chemical compound O[C@H]1COC(O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-HWQSCIPKSA-N 0.000 description 11
- 238000000246 agarose gel electrophoresis Methods 0.000 description 11
- 229940041514 candida albicans extract Drugs 0.000 description 10
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 description 10
- 238000004519 manufacturing process Methods 0.000 description 10
- 230000037361 pathway Effects 0.000 description 10
- 239000012138 yeast extract Substances 0.000 description 10
- 101150035354 araA gene Proteins 0.000 description 8
- 101150017736 araD gene Proteins 0.000 description 8
- 230000000813 microbial effect Effects 0.000 description 8
- 230000004127 xylose metabolism Effects 0.000 description 8
- 238000013019 agitation Methods 0.000 description 7
- 229960000723 ampicillin Drugs 0.000 description 7
- AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 7
- 229940106157 cellulase Drugs 0.000 description 7
- 238000011068 loading method Methods 0.000 description 7
- 230000014759 maintenance of location Effects 0.000 description 7
- 239000002028 Biomass Substances 0.000 description 6
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 6
- 229940111685 dibasic potassium phosphate Drugs 0.000 description 6
- 230000029087 digestion Effects 0.000 description 6
- 239000013605 shuttle vector Substances 0.000 description 6
- 108090000769 Isomerases Proteins 0.000 description 5
- 102000004195 Isomerases Human genes 0.000 description 5
- 238000013459 approach Methods 0.000 description 5
- 238000004520 electroporation Methods 0.000 description 5
- 230000002414 glycolytic effect Effects 0.000 description 5
- 230000004060 metabolic process Effects 0.000 description 5
- 241001131785 Escherichia coli HB101 Species 0.000 description 4
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 4
- 229920000168 Microcrystalline cellulose Polymers 0.000 description 4
- 101100157012 Thermoanaerobacterium saccharolyticum (strain DSM 8691 / JW/SL-YS485) xynB gene Proteins 0.000 description 4
- 102100033055 Transketolase Human genes 0.000 description 4
- OIRDTQYFTABQOQ-UHTZMRCNSA-N Vidarabine Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@@H]1O OIRDTQYFTABQOQ-UHTZMRCNSA-N 0.000 description 4
- 239000011543 agarose gel Substances 0.000 description 4
- OIRDTQYFTABQOQ-UHFFFAOYSA-N ara-adenosine Natural products Nc1ncnc2n(cnc12)C1OC(CO)C(O)C1O OIRDTQYFTABQOQ-UHFFFAOYSA-N 0.000 description 4
- 230000000295 complement effect Effects 0.000 description 4
- 239000002054 inoculum Substances 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 235000019813 microcrystalline cellulose Nutrition 0.000 description 4
- 239000008108 microcrystalline cellulose Substances 0.000 description 4
- 229940016286 microcrystalline cellulose Drugs 0.000 description 4
- 238000000926 separation method Methods 0.000 description 4
- 101150014795 tktA gene Proteins 0.000 description 4
- 101150110790 xylB gene Proteins 0.000 description 4
- 108020005029 5' Flanking Region Proteins 0.000 description 3
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 3
- 241001522878 Escherichia coli B Species 0.000 description 3
- 108091034117 Oligonucleotide Proteins 0.000 description 3
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 3
- 101150024271 TKT gene Proteins 0.000 description 3
- 244000309466 calf Species 0.000 description 3
- 230000006652 catabolic pathway Effects 0.000 description 3
- 238000006243 chemical reaction Methods 0.000 description 3
- 239000003795 chemical substances by application Substances 0.000 description 3
- 230000000052 comparative effect Effects 0.000 description 3
- 238000012217 deletion Methods 0.000 description 3
- 230000037430 deletion Effects 0.000 description 3
- 239000000446 fuel Substances 0.000 description 3
- 238000010353 genetic engineering Methods 0.000 description 3
- 150000002402 hexoses Chemical class 0.000 description 3
- 230000000968 intestinal effect Effects 0.000 description 3
- 238000002955 isolation Methods 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 230000001404 mediated effect Effects 0.000 description 3
- 230000001105 regulatory effect Effects 0.000 description 3
- 230000010076 replication Effects 0.000 description 3
- 238000012546 transfer Methods 0.000 description 3
- 238000011144 upstream manufacturing Methods 0.000 description 3
- 229920001817 Agar Polymers 0.000 description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 102000014914 Carrier Proteins Human genes 0.000 description 2
- 108010078791 Carrier Proteins Proteins 0.000 description 2
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 2
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 2
- 241000588748 Klebsiella Species 0.000 description 2
- ZAQJHHRNXZUBTE-UCORVYFPSA-N L-ribulose Chemical compound OC[C@H](O)[C@H](O)C(=O)CO ZAQJHHRNXZUBTE-UCORVYFPSA-N 0.000 description 2
- 229910019142 PO4 Inorganic materials 0.000 description 2
- 102000004160 Phosphoric Monoester Hydrolases Human genes 0.000 description 2
- 108090000608 Phosphoric Monoester Hydrolases Proteins 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 108091081024 Start codon Proteins 0.000 description 2
- 108010006785 Taq Polymerase Proteins 0.000 description 2
- 241000589634 Xanthomonas Species 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- 235000013334 alcoholic beverage Nutrition 0.000 description 2
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 2
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 2
- 235000011130 ammonium sulphate Nutrition 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- 230000010261 cell growth Effects 0.000 description 2
- 210000000349 chromosome Anatomy 0.000 description 2
- 238000010367 cloning Methods 0.000 description 2
- 238000010276 construction Methods 0.000 description 2
- 238000007796 conventional method Methods 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- NHVNXKFIZYSCEB-XLPZGREQSA-N dTTP Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)C1 NHVNXKFIZYSCEB-XLPZGREQSA-N 0.000 description 2
- 238000013461 design Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000002255 enzymatic effect Effects 0.000 description 2
- 101150073818 gap gene Proteins 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- 238000011534 incubation Methods 0.000 description 2
- 239000000543 intermediate Substances 0.000 description 2
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 2
- 235000019341 magnesium sulphate Nutrition 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 239000010452 phosphate Substances 0.000 description 2
- 239000013600 plasmid vector Substances 0.000 description 2
- 230000000644 propagated effect Effects 0.000 description 2
- 238000013518 transcription Methods 0.000 description 2
- 230000035897 transcription Effects 0.000 description 2
- 230000002103 transcriptional effect Effects 0.000 description 2
- 239000012137 tryptone Substances 0.000 description 2
- PKAUICCNAWQPAU-UHFFFAOYSA-N 2-(4-chloro-2-methylphenoxy)acetic acid;n-methylmethanamine Chemical compound CNC.CC1=CC(Cl)=CC=C1OCC(O)=O PKAUICCNAWQPAU-UHFFFAOYSA-N 0.000 description 1
- 241000589220 Acetobacter Species 0.000 description 1
- 241000589158 Agrobacterium Species 0.000 description 1
- 108010084185 Cellulases Proteins 0.000 description 1
- 102000005575 Cellulases Human genes 0.000 description 1
- ZAQJHHRNXZUBTE-WUJLRWPWSA-N D-xylulose Chemical compound OC[C@@H](O)[C@H](O)C(=O)CO ZAQJHHRNXZUBTE-WUJLRWPWSA-N 0.000 description 1
- FNZLKVNUWIIPSJ-RFZPGFLSSA-N D-xylulose 5-phosphate Chemical compound OCC(=O)[C@@H](O)[C@H](O)COP(O)(O)=O FNZLKVNUWIIPSJ-RFZPGFLSSA-N 0.000 description 1
- 108010017826 DNA Polymerase I Proteins 0.000 description 1
- 102000004594 DNA Polymerase I Human genes 0.000 description 1
- 101900289151 Escherichia coli Xylose isomerase Proteins 0.000 description 1
- 241001302584 Escherichia coli str. K-12 substr. W3110 Species 0.000 description 1
- 241000589565 Flavobacterium Species 0.000 description 1
- 229930091371 Fructose Natural products 0.000 description 1
- 239000005715 Fructose Substances 0.000 description 1
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 description 1
- 108700039691 Genetic Promoter Regions Proteins 0.000 description 1
- 241000589236 Gluconobacter Species 0.000 description 1
- 101100175482 Glycine max CG-3 gene Proteins 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 108091081548 Palindromic sequence Proteins 0.000 description 1
- 102000045595 Phosphoprotein Phosphatases Human genes 0.000 description 1
- 108700019535 Phosphoprotein Phosphatases Proteins 0.000 description 1
- 101150057388 Reln gene Proteins 0.000 description 1
- 241000589180 Rhizobium Species 0.000 description 1
- 241000191025 Rhodobacter Species 0.000 description 1
- 101150010882 S gene Proteins 0.000 description 1
- 240000000111 Saccharum officinarum Species 0.000 description 1
- 235000007201 Saccharum officinarum Nutrition 0.000 description 1
- 241000607142 Salmonella Species 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 238000010923 batch production Methods 0.000 description 1
- 230000008238 biochemical pathway Effects 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 238000010960 commercial process Methods 0.000 description 1
- 230000021615 conjugation Effects 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 235000005822 corn Nutrition 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 230000002939 deleterious effect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 238000004821 distillation Methods 0.000 description 1
- 101150107963 eno gene Proteins 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 238000012262 fermentative production Methods 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 238000005189 flocculation Methods 0.000 description 1
- 230000016615 flocculation Effects 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 230000005714 functional activity Effects 0.000 description 1
- 238000012215 gene cloning Methods 0.000 description 1
- 230000004077 genetic alteration Effects 0.000 description 1
- 231100000118 genetic alteration Toxicity 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 229940059442 hemicellulase Drugs 0.000 description 1
- 108010002430 hemicellulase Proteins 0.000 description 1
- 238000004128 high performance liquid chromatography Methods 0.000 description 1
- 239000000411 inducer Substances 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 229920005610 lignin Polymers 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000002703 mutagenesis Methods 0.000 description 1
- 231100000350 mutagenesis Toxicity 0.000 description 1
- 230000035772 mutation Effects 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 235000020038 palm wine Nutrition 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 235000019989 pulque Nutrition 0.000 description 1
- 230000003252 repetitive effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000004062 sedimentation Methods 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000000638 solvent extraction Methods 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 230000026683 transduction Effects 0.000 description 1
- 238000010361 transduction Methods 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/06—Ethanol, i.e. non-beverage
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Molecular Biology (AREA)
- Plant Pathology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US08/421,996 US5726053A (en) | 1994-04-15 | 1995-04-14 | Recombinant Zymomonas for pentose fermentation |
US08/422,424 US5712133A (en) | 1994-04-15 | 1995-04-14 | Pentose fermentation by recombinant zymomonas |
US08/421,996 | 1995-04-14 | ||
US08/422,424 | 1995-04-14 |
Publications (2)
Publication Number | Publication Date |
---|---|
CA2173793A1 CA2173793A1 (en) | 1996-10-15 |
CA2173793C true CA2173793C (en) | 2011-01-11 |
Family
ID=27025447
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA2173793A Expired - Lifetime CA2173793C (en) | 1995-04-14 | 1996-04-10 | Pentose fermentation by recombinant zymomonas |
Country Status (3)
Country | Link |
---|---|
KR (1) | KR100455096B1 (ko) |
AU (1) | AU698662B2 (ko) |
CA (1) | CA2173793C (ko) |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
AU774414B2 (en) * | 1997-05-06 | 2004-06-24 | Midwest Research Institute | Single (zymomonas mobilis) strain for xylose and arabinose fermentation |
-
1996
- 1996-04-10 CA CA2173793A patent/CA2173793C/en not_active Expired - Lifetime
- 1996-04-11 AU AU50582/96A patent/AU698662B2/en not_active Expired
- 1996-04-13 KR KR1019960011100A patent/KR100455096B1/ko not_active IP Right Cessation
Also Published As
Publication number | Publication date |
---|---|
KR100455096B1 (ko) | 2005-01-13 |
KR960037834A (ko) | 1996-11-19 |
AU5058296A (en) | 1996-10-24 |
CA2173793A1 (en) | 1996-10-15 |
AU698662B2 (en) | 1998-11-05 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
US5712133A (en) | Pentose fermentation by recombinant zymomonas | |
US5514583A (en) | Recombinant zymomonas for pentose fermentation | |
EP0737742A2 (en) | Pentose fermentation by recombinant zymomonas | |
US5843760A (en) | Single zymomonas mobilis strain for xylose and arabinose fermentation | |
Chandrakant et al. | Simultaneous bioconversion of cellulose and hemicellulose to ethanol | |
Deanda et al. | Development of an arabinose-fermenting Zymomonas mobilis strain by metabolic pathway engineering | |
Krishnan et al. | Fermentation kinetics of ethanol production from glucose and xylose by recombinant Saccharomyces 1400 (pLNH33) | |
US6566107B1 (en) | Recombinant Zymomonas mobilis with improved xylose utilization | |
Gunasekaran et al. | Ethanol fermentation technology–Zymomonas mobilis | |
CN105199976B (zh) | 一株共发酵葡萄糖和木糖的重组酿酒酵母菌株及其应用 | |
WO2007005646A2 (en) | Recombinant host cells and media for ethanol production | |
US5798237A (en) | Recombinant lactobacillus for fermentation of xylose to lactic acid and lactate | |
JP4124270B1 (ja) | グルコース・マンノース・キシロース並行発酵性菌およびそれを用いるバイオエタノールの製造方法 | |
WO2010147835A1 (en) | Zymomonas with improved arabinose utilization | |
US5726053A (en) | Recombinant Zymomonas for pentose fermentation | |
de Carvalho Lima et al. | Ethanol production from corn cob hydrolysates by Escherichia coli KO11 | |
CN108220323B (zh) | 利用甲醇的产油酵母工程菌株构建方法及工程菌和应用 | |
Su et al. | Cloning and expression of a β-glucosidase gene from Xanthomonas albilineans in Escherichia coli and Zymomonas mobilis | |
Xia et al. | Combined strategy of transcription factor manipulation and β-glucosidase gene overexpression in Trichoderma reesei and its application in lignocellulose bioconversion | |
US11091782B2 (en) | Engineered zymomonas for the production of 2,3-butanediol | |
CN116925987A (zh) | 一种经磷壁酸合成强化的高产抗逆菌株及其构建方法与多元醇发酵应用 | |
CA2173793C (en) | Pentose fermentation by recombinant zymomonas | |
Picataggio et al. | Development of genetically engineered microorganisms for ethanol production | |
WO2009081941A1 (ja) | 木質系および草本系バイオマス糖化液からのバイオエタノール並行発酵菌 | |
CN114874961B (zh) | 一种利用乙醛合成乙偶姻的重组运动发酵单胞菌及其构建方法与应用 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
EEER | Examination request | ||
MKEX | Expiry |
Effective date: 20160411 |