CA2168091A1 - Croissance in vitro de precurseurs neutrophiles et de precurseurs de megacaryocytes dans un milieu exempt de serum - Google Patents

Croissance in vitro de precurseurs neutrophiles et de precurseurs de megacaryocytes dans un milieu exempt de serum

Info

Publication number
CA2168091A1
CA2168091A1 CA002168091A CA2168091A CA2168091A1 CA 2168091 A1 CA2168091 A1 CA 2168091A1 CA 002168091 A CA002168091 A CA 002168091A CA 2168091 A CA2168091 A CA 2168091A CA 2168091 A1 CA2168091 A1 CA 2168091A1
Authority
CA
Canada
Prior art keywords
cells
serum
free
megakaryocyte
cell
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002168091A
Other languages
English (en)
Inventor
Stephen L. Smith
Xiaoying Qiao
Susan M. Maciukas
James G. Bender
Dennis E. Van Epps
Maureen F. Loudovaris
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Nexell Therapeutics Inc
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2168091A1 publication Critical patent/CA2168091A1/fr
Abandoned legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0644—Platelets; Megakaryocytes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0642—Granulocytes, e.g. basopils, eosinophils, neutrophils, mast cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
    • C12N2500/24—Iron; Fe chelators; Transferrin
    • C12N2500/25—Insulin-transferrin; Insulin-transferrin-selenium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/36—Lipids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/44—Thiols, e.g. mercaptoethanol
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/98—Xeno-free medium and culture conditions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/125—Stem cell factor [SCF], c-kit ligand [KL]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/22—Colony stimulating factors (G-CSF, GM-CSF)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/23—Interleukins [IL]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/38—Hormones with nuclear receptors
    • C12N2501/39—Steroid hormones

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Hematology (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

On décrit des compositions de milieux exempts de sérum et de protéines animales, devant être utilisées conjointement avec des facteurs de croissance hématopoiétique pour la croissance in vitro de précurseurs de neutrophiles et de mégacaryocytes humains. Le milieu est composé d'un milieu de base, d'un corticostéroïde, de transferrine, d'insuline, de cholestérol, d'éthanolamine et d'albumine humaine. L'invention concerne également des procédés de préparation de suspensions, exemptes de sérum et de protéines, animales, de cellules précurseurs hématopoiétiques humaines, dans lesquelles le composant cellulaire comprend au moins 16 % environ de précurseurs de neutrophiles et au moins 1 % environ de précurseurs de mégacaryocytes. On décrit des suspensions exemptes de sérum et de protéines, animales, dans lesquelles le composant cellulaire comprend au moins 30 % environ, et de préférence plus de 60 % de précurseurs de neutrophiles. Les précurseurs de neutrophiles comprennent des cellules blastiques, des promyélocytes, des myélocytes neutrophiles et des métamyélocytes neutrophiles. On décrit également des suspensions de cellules exemptes de sérum et de protéines, animales, dans lesquelles le composant cellulaire comprend au moins 3 % environ et de préférence plus de 8 % de précurseurs de mégacaryocytes, ainsi que des suspensions de cellules exemptes de sérum et de protéines animales, dans lesquelles le composant cellulaire comprend des unités formant des colonies et des unités formant des grappes.
CA002168091A 1993-08-23 1994-08-23 Croissance in vitro de precurseurs neutrophiles et de precurseurs de megacaryocytes dans un milieu exempt de serum Abandoned CA2168091A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US11027793A 1993-08-23 1993-08-23
US08/110,277 1993-08-23

Publications (1)

Publication Number Publication Date
CA2168091A1 true CA2168091A1 (fr) 1995-03-02

Family

ID=22332160

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002168091A Abandoned CA2168091A1 (fr) 1993-08-23 1994-08-23 Croissance in vitro de precurseurs neutrophiles et de precurseurs de megacaryocytes dans un milieu exempt de serum

Country Status (7)

Country Link
EP (1) EP0719326A1 (fr)
JP (1) JPH09505462A (fr)
AU (1) AU702871B2 (fr)
CA (1) CA2168091A1 (fr)
IL (1) IL110755A (fr)
SG (1) SG52619A1 (fr)
WO (1) WO1995006112A1 (fr)

Families Citing this family (28)

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Publication number Priority date Publication date Assignee Title
US6248319B1 (en) 1989-10-16 2001-06-19 Krisztina M. Zsebo Method for increasing hematopoietic progenitor cells by stem cell factor polypeptides
US6207454B1 (en) 1989-10-16 2001-03-27 Amgen Inc. Method for enhancing the effciency of gene transfer with stem cell factor (SCF) polypeptide
US6852313B1 (en) 1989-10-16 2005-02-08 Amgen Inc. Method of stimulating growth of melanocyte cells by administering stem cell factor
US7144731B2 (en) 1989-10-16 2006-12-05 Amgen Inc. SCF antibody compositions and methods of using the same
US9068164B1 (en) * 1995-02-02 2015-06-30 Tristem Trading (Cyprus) Limited Method of preparing an undifferentiated cell
AU6125396A (en) * 1995-06-07 1996-12-30 Novartis Ag Serum-free media for primitive hematopoietic cells and metho ds of use thereof
WO1997033978A1 (fr) * 1996-03-12 1997-09-18 Life Technologies, Inc. Additif pour milieu de culture nutritif pour cellules hematopoietiques
AU4330597A (en) 1996-08-30 1998-03-19 Life Technologies, Inc. Serum-free mammalian cell culture medium, and uses thereof
US5945337A (en) 1996-10-18 1999-08-31 Quality Biological, Inc. Method for culturing CD34+ cells in a serum-free medium
US6811776B2 (en) 2000-12-27 2004-11-02 The Regents Of The University Of Michigan Process for ex vivo formation of mammalian bone and uses thereof
WO2005063968A1 (fr) * 2003-12-26 2005-07-14 Gs Platz Co Ltd Milieu de base pour la culture de cellules es
JP4651282B2 (ja) 2004-01-21 2011-03-16 田辺三菱製薬株式会社 造血幹細胞及び造血前駆細胞の増幅方法
US7923013B2 (en) 2004-12-28 2011-04-12 The Rockefeller University Glycolipids and analogues thereof as antigens for NKT cells
CA2591232C (fr) 2004-12-28 2014-10-21 The Rockefeller University Glycolipides et analogues associes utilises en tant qu'antigenes des lymphocytes nkt
US7837990B2 (en) 2005-03-28 2010-11-23 The Rockefeller University In vivo expanded NKT cells and methods of use thereof
EP3424507A1 (fr) 2006-03-24 2019-01-09 Children's Medical Center Corporation Procédé de modulation de croissance de cellules souches hématopoïétiques
AU2015207905B2 (en) * 2006-03-24 2017-04-27 Children's Medical Center Corporation Method to modulate hematopoietic stem cell growth
AU2013202353B2 (en) * 2006-03-24 2015-05-07 Children's Medical Center Corporation Method to modulate hematopoietic stem cell growth
JP5426389B2 (ja) 2006-10-20 2014-02-26 チルドレンズ メディカル センター コーポレーション 組織の再生を亢進するための方法
WO2010091052A2 (fr) 2009-02-03 2010-08-12 Children's Medical Center Corporation Procédés de renforcement de greffe de cellule souche hématopoïétique/progénitrice
US9056085B2 (en) 2009-02-03 2015-06-16 Children's Medical Center Corporation Methods for enhancing hematopoietic stem/progenitor cell engraftment
EP2729560A4 (fr) * 2011-07-06 2014-12-03 Cellerant Therapeutics Inc Cellules progénitrices mégacaryocytaires pour la production de plaquettes
EP2785350B1 (fr) 2011-12-02 2018-05-02 Fate Therapeutics, Inc. Méthodes améliorées permettant de traiter l'ischémie
CN104066837B (zh) 2011-12-02 2020-12-08 菲特治疗公司 增强的干细胞组合物
EP2882846B1 (fr) 2012-06-05 2018-09-05 The Regents of the University of California Procédés et compositions pour la production rapide de cellules épithéliales pigmentées de la rétine à partir de cellules multipotentes
EP2968416A4 (fr) 2013-03-15 2016-08-17 Fate Therapeutics Inc Essai d'activité biologique de cellules pour un potentiel thérapeutique
JP2021180620A (ja) * 2020-05-18 2021-11-25 株式会社マイオリッジ 目的細胞の生産方法、目的細胞による生産物の生産方法、および無血清培地
FR3128874A1 (fr) * 2021-11-09 2023-05-12 Crossject Solution pharmaceutique d’hydrocortisone pour dispositif d’injection

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5004681B1 (en) * 1987-11-12 2000-04-11 Biocyte Corp Preservation of fetal and neonatal hematopoietic stem and progenitor cells of the blood
WO1992006178A1 (fr) * 1990-10-05 1992-04-16 Systemix, Inc. Croissance, production et composition des megacaryocytes et des plaquettes

Also Published As

Publication number Publication date
AU702871B2 (en) 1999-03-11
IL110755A0 (en) 1994-11-11
WO1995006112A1 (fr) 1995-03-02
JPH09505462A (ja) 1997-06-03
SG52619A1 (en) 1998-09-28
AU7674494A (en) 1995-03-21
IL110755A (en) 2000-11-21
EP0719326A1 (fr) 1996-07-03

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