SG52619A1 - In vitro growth of neutrophil and megakaryocte precursors in serum-free media - Google Patents

In vitro growth of neutrophil and megakaryocte precursors in serum-free media

Info

Publication number
SG52619A1
SG52619A1 SG1996006929A SG1996006929A SG52619A1 SG 52619 A1 SG52619 A1 SG 52619A1 SG 1996006929 A SG1996006929 A SG 1996006929A SG 1996006929 A SG1996006929 A SG 1996006929A SG 52619 A1 SG52619 A1 SG 52619A1
Authority
SG
Singapore
Prior art keywords
megakaryocte
neutrophil
precursors
serum
free media
Prior art date
Application number
SG1996006929A
Inventor
Stephen L Smith
Kiaoying Qiao
Susan M Maciukas
James G Bender
Van Dennis E Epps
Maureen F Loudovaris
Original Assignee
Baxter Int
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Baxter Int filed Critical Baxter Int
Publication of SG52619A1 publication Critical patent/SG52619A1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0644—Platelets; Megakaryocytes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0642—Granulocytes, e.g. basopils, eosinophils, neutrophils, mast cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
    • C12N2500/24—Iron; Fe chelators; Transferrin
    • C12N2500/25—Insulin-transferrin; Insulin-transferrin-selenium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/36—Lipids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/44—Thiols, e.g. mercaptoethanol
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/98—Xeno-free medium and culture conditions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/125—Stem cell factor [SCF], c-kit ligand [KL]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/22—Colony stimulating factors (G-CSF, GM-CSF)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/23—Interleukins [IL]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/38—Hormones with nuclear receptors
    • C12N2501/39—Steroid hormones

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Hematology (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
SG1996006929A 1993-08-23 1994-08-23 In vitro growth of neutrophil and megakaryocte precursors in serum-free media SG52619A1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US11027793A 1993-08-23 1993-08-23

Publications (1)

Publication Number Publication Date
SG52619A1 true SG52619A1 (en) 1998-09-28

Family

ID=22332160

Family Applications (1)

Application Number Title Priority Date Filing Date
SG1996006929A SG52619A1 (en) 1993-08-23 1994-08-23 In vitro growth of neutrophil and megakaryocte precursors in serum-free media

Country Status (7)

Country Link
EP (1) EP0719326A1 (en)
JP (1) JPH09505462A (en)
AU (1) AU702871B2 (en)
CA (1) CA2168091A1 (en)
IL (1) IL110755A (en)
SG (1) SG52619A1 (en)
WO (1) WO1995006112A1 (en)

Families Citing this family (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6207454B1 (en) 1989-10-16 2001-03-27 Amgen Inc. Method for enhancing the effciency of gene transfer with stem cell factor (SCF) polypeptide
US6248319B1 (en) 1989-10-16 2001-06-19 Krisztina M. Zsebo Method for increasing hematopoietic progenitor cells by stem cell factor polypeptides
US7144731B2 (en) 1989-10-16 2006-12-05 Amgen Inc. SCF antibody compositions and methods of using the same
US6852313B1 (en) 1989-10-16 2005-02-08 Amgen Inc. Method of stimulating growth of melanocyte cells by administering stem cell factor
US9068164B1 (en) * 1995-02-02 2015-06-30 Tristem Trading (Cyprus) Limited Method of preparing an undifferentiated cell
WO1996040866A1 (en) * 1995-06-07 1996-12-19 Novartis Ag Serum-free media for primitive hematopoietic cells and methods of use thereof
US6733746B2 (en) * 1996-03-12 2004-05-11 Invitrogen Corporation Hematopoietic cell culture nutrient supplement
JP2000517188A (en) 1996-08-30 2000-12-26 ライフ テクノロジーズ，インコーポレイテッド Serum-free mammalian cell culture medium and uses thereof
US5945337A (en) * 1996-10-18 1999-08-31 Quality Biological, Inc. Method for culturing CD34+ cells in a serum-free medium
US6811776B2 (en) 2000-12-27 2004-11-02 The Regents Of The University Of Michigan Process for ex vivo formation of mammalian bone and uses thereof
ATE466073T1 (en) * 2003-12-26 2010-05-15 Makoto Asashima BASAL MEDIUM FOR CULTIVATION OF ES CELLS
JP4651282B2 (en) * 2004-01-21 2011-03-16 田辺三菱製薬株式会社 Method for amplifying hematopoietic stem cells and hematopoietic progenitor cells
WO2006071848A2 (en) 2004-12-28 2006-07-06 The Rockefeller University Glycolipids and analogues thereof as antigens for nk t cells
US7923013B2 (en) 2004-12-28 2011-04-12 The Rockefeller University Glycolipids and analogues thereof as antigens for NKT cells
US7837990B2 (en) 2005-03-28 2010-11-23 The Rockefeller University In vivo expanded NKT cells and methods of use thereof
AU2013202353B2 (en) * 2006-03-24 2015-05-07 Children's Medical Center Corporation Method to modulate hematopoietic stem cell growth
AU2015207905B2 (en) * 2006-03-24 2017-04-27 Children's Medical Center Corporation Method to modulate hematopoietic stem cell growth
ZA200808123B (en) 2006-03-24 2010-03-31 Childrens Medical Center Method to modulate hematopoietic stem cell growth
EP2077826B1 (en) 2006-10-20 2013-09-04 Children's Medical Center Corporation Method to enhance tissue regeneration
WO2010091052A2 (en) 2009-02-03 2010-08-12 Children's Medical Center Corporation Methods for enhancing hematopoietic stem/progenitor cell engraftment
WO2010096264A2 (en) 2009-02-03 2010-08-26 Children's Medical Center Corporation Methods for enhancing hematopoietic stem/progenitor cell engraftment
JP2014523741A (en) * 2011-07-06 2014-09-18 セレラント セラピューティクス インコーポレイテッド Megakaryocyte progenitor cells for platelet production
EP2785834B1 (en) 2011-12-02 2020-09-23 Fate Therapeutics, Inc. Enhanced stem cell composition
EP2785350B1 (en) 2011-12-02 2018-05-02 Fate Therapeutics, Inc. Improved methods of treating ischemia
WO2013184809A1 (en) * 2012-06-05 2013-12-12 The Regents Of The University Of California Methods and compositions for the rapid production of retinal pigmented epithelial cells from pluripotent cells
US10851412B2 (en) 2013-03-15 2020-12-01 Fate Therapeutics, Inc. Cell potency assay for therapeutic potential
JP2021180620A (en) * 2020-05-18 2021-11-25 株式会社マイオリッジ Method for producing target cell, method for producing product with target cell, and serum-free medium
FR3128874A1 (en) * 2021-11-09 2023-05-12 Crossject Pharmaceutical solution of hydrocortisone for injection device

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5004681B1 (en) * 1987-11-12 2000-04-11 Biocyte Corp Preservation of fetal and neonatal hematopoietic stem and progenitor cells of the blood
EP0504361A4 (en) * 1990-10-05 1994-08-17 Systemix Inc Megakaryocyte and platelet growth, production and composition

Also Published As

Publication number Publication date
JPH09505462A (en) 1997-06-03
WO1995006112A1 (en) 1995-03-02
AU7674494A (en) 1995-03-21
AU702871B2 (en) 1999-03-11
IL110755A (en) 2000-11-21
IL110755A0 (en) 1994-11-11
EP0719326A1 (en) 1996-07-03
CA2168091A1 (en) 1995-03-02

Similar Documents

Publication Publication Date Title
SG52619A1 (en) In vitro growth of neutrophil and megakaryocte precursors in serum-free media
HK3996A (en) Diamond having multiple coatings methods for their manufacture and use of same
ZA9210123B (en) Coated abrasive article and method of making same
AU4105796A (en) Signage articles and methods of making same
IL114397A0 (en) Growth/differentiation factor of the TGF-beta-family
EP0527781A4 (en) Methods and formulations for stabilizing fibroblast growth factor
ZA949025B (en) Formed incontinence article and method of manufacture
AU651959B2 (en) Sintered aluminous abrasive and method of producing same
HU9401437D0 (en) Use of multi-staged betex and process for meting the multi-steged betex
AU6125396A (en) Serum-free media for primitive hematopoietic cells and metho ds of use thereof
AU3208793A (en) Abrasive article and method
EP0515186A3 (en) Densified porous billets and process
AU3937593A (en) Trans-sialidase and methods of use and making thereof
AU7787191A (en) Improved bioreactor surfaces and methods of making same
ZA919259B (en) Active fragments of fibroblast growth factor
GB2271884B (en) Solenoids and methods of making solenoids
GB9200401D0 (en) Method of making precursors and articles of ceramic-reinforced metal composites
HU9300365D0 (en) Oxide based granules of high hardness coated with colour ceramics and method for producing them
AU3139593A (en) Synthesis of taxol in culture
AU2846792A (en) Process for preparing sintered shapes and compositions for use therein
AU8057791A (en) Type-c rotavirus cultures and uses therefor
AU5300594A (en) Medium for in vitro fertilization and method of in vitro fertilization
AU3555993A (en) Antibiotic preparation and use of the same in plants
AU2062497A (en) Antisense interleukin 10 and methods of use
AU7112194A (en) Inhibition of basic fibroblast growth factor with oligonculeotides