CA2111984A1 - Additif d'un milieu de culture contenant un agent chelateur du fer - Google Patents

Additif d'un milieu de culture contenant un agent chelateur du fer

Info

Publication number
CA2111984A1
CA2111984A1 CA 2111984 CA2111984A CA2111984A1 CA 2111984 A1 CA2111984 A1 CA 2111984A1 CA 2111984 CA2111984 CA 2111984 CA 2111984 A CA2111984 A CA 2111984A CA 2111984 A1 CA2111984 A1 CA 2111984A1
Authority
CA
Canada
Prior art keywords
citrate
iron
serum
culture medium
free
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA 2111984
Other languages
English (en)
Inventor
Peter B. Suhr-Jessen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novo Nordisk AS
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2111984A1 publication Critical patent/CA2111984A1/fr
Abandoned legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018Culture media for cell or tissue culture
    • C12N5/005Protein-free medium
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00Specific components of cell culture medium
    • C12N2500/05Inorganic components
    • C12N2500/10Metals; Metal chelators
    • C12N2500/20Transition metals
    • C12N2500/24Iron; Fe chelators; Transferrin

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CA 2111984 1991-06-21 1992-06-18 Additif d'un milieu de culture contenant un agent chelateur du fer Abandoned CA2111984A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP91610054.8 1991-06-21
EP91610054 1991-06-21

Publications (1)

Publication Number Publication Date
CA2111984A1 true CA2111984A1 (fr) 1993-01-07

Family

ID=8208780

Family Applications (1)

Application Number Title Priority Date Filing Date
CA 2111984 Abandoned CA2111984A1 (fr) 1991-06-21 1992-06-18 Additif d'un milieu de culture contenant un agent chelateur du fer

Country Status (5)

Country Link
EP (1) EP0593539A1 (fr)
JP (1) JPH06508523A (fr)
AU (1) AU2196892A (fr)
CA (1) CA2111984A1 (fr)
WO (1) WO1993000423A1 (fr)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2243827B2 (fr) 1996-08-30 2017-11-22 Life Technologies Corporation Milieu de culture de cellules de mammiferes exempt de serum
JP4972261B2 (ja) * 1999-08-27 2012-07-11 ライフ テクノロジーズ コーポレーション 金属結合化合物および細胞培養培地組成物中でのそれらの使用
US6767741B1 (en) 1999-08-27 2004-07-27 Invitrogen Corporation Metal binding compounds and their use in cell culture medium compositions
US20030096414A1 (en) 2001-03-27 2003-05-22 Invitrogen Corporation Culture medium for cell growth and transfection
CA2417689C (fr) 2002-03-05 2006-05-09 F. Hoffmann-La Roche Ag Methodes ameliorees de culture in vitro de cellules de mammiferes
ATE359359T1 (de) * 2003-08-08 2007-05-15 Cambridge Antibody Tech Myeloma zellkultur in einem transferrin-freien medium mit niedrigem eisengehalt
GB2404665B (en) 2003-08-08 2005-07-06 Cambridge Antibody Tech Cell culture
EP1812557A4 (fr) 2004-10-29 2009-11-04 Centocor Ortho Biotech Inc Compositions de milieux chimiquement definis
US8637312B2 (en) 2008-01-09 2014-01-28 Cellca Gmbh Mammalian culture media with polyamine and iron
SG11201401220SA (en) * 2011-10-21 2014-07-30 Pfizer Addition of iron to improve cell culture
EP3778868B1 (fr) * 2019-08-16 2022-01-26 UGA Biopharma GmbH Milieu de culture cellulaire permettant de cultiver des cellules, procédé de culture des cellules et procédé d'expression d'au moins une protéine recombinant dans une culture cellulaire

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CS262822B1 (en) * 1986-10-03 1989-04-14 Kovar Jan Synthetic medium for the cultivation of myelomic cells
NO162160C (no) * 1987-01-09 1989-11-15 Medi Cult As Serumfritt vekstmedium, samt anvendelse derav.

Also Published As

Publication number Publication date
WO1993000423A1 (fr) 1993-01-07
JPH06508523A (ja) 1994-09-29
AU2196892A (en) 1993-01-25
EP0593539A1 (fr) 1994-04-27

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Legal Events

Date Code Title Description
FZDE Discontinued
FZDE Discontinued

Effective date: 19980618