CA2091443A1 - Milieu de culture de cellules de mammiferes - Google Patents

Milieu de culture de cellules de mammiferes

Info

Publication number
CA2091443A1
CA2091443A1 CA 2091443 CA2091443A CA2091443A1 CA 2091443 A1 CA2091443 A1 CA 2091443A1 CA 2091443 CA2091443 CA 2091443 CA 2091443 A CA2091443 A CA 2091443A CA 2091443 A1 CA2091443 A1 CA 2091443A1
Authority
CA
Canada
Prior art keywords
per liter
medium
milligrams per
serum
amount
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA 2091443
Other languages
English (en)
Inventor
Luciano Ramos
Amy A. Murnane
Melvin S. Oka
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
SmithKline Beecham Corp
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2091443A1 publication Critical patent/CA2091443A1/fr
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C12N5/0037—Serum-free medium, which may still contain naturally-sourced components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
    • C12N2500/24—Iron; Fe chelators; Transferrin
    • C12N2500/25—Insulin-transferrin; Insulin-transferrin-selenium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/34—Sugars
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/36—Lipids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/70—Undefined extracts
    • C12N2500/74—Undefined extracts from fungi, e.g. yeasts

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CA 2091443 1990-09-25 1991-09-20 Milieu de culture de cellules de mammiferes Abandoned CA2091443A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US58801790A 1990-09-25 1990-09-25
US07/588,017 1990-09-25

Publications (1)

Publication Number Publication Date
CA2091443A1 true CA2091443A1 (fr) 1992-03-26

Family

ID=24352124

Family Applications (1)

Application Number Title Priority Date Filing Date
CA 2091443 Abandoned CA2091443A1 (fr) 1990-09-25 1991-09-20 Milieu de culture de cellules de mammiferes

Country Status (11)

Country Link
EP (1) EP0550638A4 (fr)
JP (1) JPH06500918A (fr)
AU (1) AU662491B2 (fr)
CA (1) CA2091443A1 (fr)
IE (1) IE913345A1 (fr)
MX (1) MX9101254A (fr)
NZ (1) NZ239900A (fr)
PT (1) PT99048B (fr)
TW (1) TW240247B (fr)
WO (1) WO1992005246A1 (fr)
ZA (1) ZA917561B (fr)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SE501217C2 (sv) * 1990-12-06 1994-12-12 Skandigen Ab Cellproliferationsmatris och användning därav
SE9303601D0 (sv) * 1993-11-01 1993-11-01 Kabi Pharmacia Ab Improved cell cultivation method and medium
JP2000507812A (ja) 1996-03-12 2000-06-27 ライフ テクノロジーズ，インコーポレイテッド 造血細胞培養栄養補充成分
EP2218775B1 (fr) 1996-08-30 2015-01-28 Life Technologies Corporation Méthode de production d'un polypeptide in vitro dans une cellule de mammifère dans un milieu de culture sans sérum and sans protéines
WO1998015614A1 (fr) * 1996-10-10 1998-04-16 Life Technologies, Inc. Milieux de culture de cellules animales comprenant des substances nutritives derivees de plantes
US6692961B1 (en) 1996-10-11 2004-02-17 Invitrogen Corporation Defined systems for epithelial cell culture and use thereof
EP2204452A1 (fr) 2000-11-23 2010-07-07 Bavarian Nordic A/S Variant du virus Modified Vaccinia Ankara
US7445924B2 (en) 2000-11-23 2008-11-04 Bavarian Nordic A/S Modified Vaccinia Ankara virus variant and cultivation method
JP2005537793A (ja) 2002-09-05 2005-12-15 バヴァリアン・ノルディック・アクティーゼルスカブ 無血清条件下で初代細胞を培養する方法及びウイルスを増幅させる方法
GB2404665B (en) 2003-08-08 2005-07-06 Cambridge Antibody Tech Cell culture
KR101050176B1 (ko) * 2003-08-08 2011-07-19 메디뮨 리미티드 트랜스페린 무함유 및 저농도의 철 함유 배지 중에서의골수종 세포 배양
WO2020127613A1 (fr) * 2018-12-21 2020-06-25 Genfit Modèle in vitro de stéatose hépatique et de stéatohépatite non alcoolique fibrosante
CN110343666B (zh) * 2019-07-10 2023-05-30 通化东宝药业股份有限公司 一种cho细胞培养的补料培养基及其制备方法和应用

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2543158B1 (fr) * 1983-03-24 1985-11-15 Inst Nat Sante Rech Med Milieu de culture de cellules animales sans serum, sans hormones et sans facteurs de croissance et procedes de culture primaire et d'obtention de lignees cellulaires utilisant ce milieu
US5024947A (en) * 1987-07-24 1991-06-18 Cetus Corporation Serum free media for the growth on insect cells and expression of products thereby
DE3801236A1 (de) * 1988-01-18 1989-07-27 Boehringer Mannheim Gmbh Pentosansulfat-medium
AU4254489A (en) * 1989-10-03 1991-04-11 Ajinomoto Co., Inc. Method of induction and activation of cytotoxic t cells
EP0550678B1 (fr) * 1990-09-25 1997-12-29 Smithkline Beecham Corporation Milieux de culture de cellules de drosophila

Also Published As

Publication number Publication date
EP0550638A1 (fr) 1993-07-14
MX9101254A (es) 1992-05-04
AU662491B2 (en) 1995-09-07
NZ239900A (en) 1993-09-27
WO1992005246A1 (fr) 1992-04-02
PT99048A (pt) 1992-08-31
IE913345A1 (en) 1992-02-25
EP0550638A4 (en) 1993-12-08
JPH06500918A (ja) 1994-01-27
AU8734891A (en) 1992-04-15
PT99048B (pt) 1999-02-26
TW240247B (fr) 1995-02-11
ZA917561B (en) 1992-09-30

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Legal Events

Date Code Title Description
EEER Examination request
FZDE Discontinued