CA2069964A1 - Methode de reproduction de coniferes par embryogenese a partir de cellules somatiques - Google Patents

Methode de reproduction de coniferes par embryogenese a partir de cellules somatiques

Info

Publication number
CA2069964A1
CA2069964A1 CA2069964A CA2069964A CA2069964A1 CA 2069964 A1 CA2069964 A1 CA 2069964A1 CA 2069964 A CA2069964 A CA 2069964A CA 2069964 A CA2069964 A CA 2069964A CA 2069964 A1 CA2069964 A1 CA 2069964A1
Authority
CA
Canada
Prior art keywords
proembryos
late stage
abscisic acid
somatic embryogenesis
cultured
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA2069964A
Other languages
English (en)
Other versions
CA2069964C (fr
Inventor
Gerald S. Pullman
Pramod K. Gupta
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Weyerhaeuser NR Co
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US07/426,331 external-priority patent/US5034326A/en
Priority claimed from US07/499,151 external-priority patent/US5036007A/en
Application filed by Individual filed Critical Individual
Publication of CA2069964A1 publication Critical patent/CA2069964A1/fr
Priority claimed from PCT/US1993/007803 external-priority patent/WO1995005070A1/fr
Application granted granted Critical
Publication of CA2069964C publication Critical patent/CA2069964C/fr
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/04—Plant cells or tissues
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C12N5/0025—Culture media for plant cell or plant tissue culture

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Cell Biology (AREA)
  • Botany (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

L'invention est une méthode de reproduction des conifères par embryogenèse somatique à l'aide de la culture de tissus végétaux. Elle comprend un procédé à étapes multiples dans lequel un embryon fécondé immature est excisé et cultivé pour induire des proembryons de stade précoce, dont la culture peut se poursuivre plus loin dans des milieux liquides sans auxines ni cytokinines, mais avec addition d'acide abscissique, pour obtenir des proembryons de stade avancé. Chez certaines espèces, il est souhaitable d'augmenter substantiellement le potentiel osmotique du milieu de développement des proembryons de stade avancé. Le milieu peut contenir de l'acide abscissique exogène et doit contenir une matière absorbante. Après plusieurs semaines en culture, la vigueur et la morphologie des embryons développés par le procédé sont améliorés et la tendance à la germination prématurée est réduite. Les embryons somatiques peuvent germer avant ou après l'entreposage et être transplantés dans le sol pour poursuivre leur croissance.
CA002069964A 1989-10-23 1990-10-22 Methode de reproduction de coniferes par embryogenese a partir de cellules somatiques Expired - Lifetime CA2069964C (fr)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US07/426,331 US5034326A (en) 1989-10-23 1989-10-23 Method for reproducing coniferous plants by somatic embryogenesis using adsorbent materials in the development stage media
US426,331 1989-10-23
US499,151 1990-03-26
US07/499,151 US5036007A (en) 1989-03-09 1990-03-26 Method for reproducing coniferous plants by somatic embryogenesis using abscisic acid and osmotic potential variation
PCT/US1993/007803 WO1995005070A1 (fr) 1989-03-09 1993-08-18 Procede de reproduction de coniferes par embryogenese somatique a l'aide d'hormones de croissance melangees utilise dans un milieu de culture d'embryons

Publications (2)

Publication Number Publication Date
CA2069964A1 true CA2069964A1 (fr) 1991-04-24
CA2069964C CA2069964C (fr) 1997-12-30

Family

ID=27027010

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002069964A Expired - Lifetime CA2069964C (fr) 1989-10-23 1990-10-22 Methode de reproduction de coniferes par embryogenese a partir de cellules somatiques

Country Status (4)

Country Link
AU (2) AU654939B2 (fr)
CA (1) CA2069964C (fr)
NZ (1) NZ235795A (fr)
WO (1) WO1991005854A1 (fr)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4957866A (en) * 1989-03-09 1990-09-18 Weyerhaeuser Company Method for reproducing coniferous plants by somatic embryogenesis
AU680206B2 (en) * 1989-03-09 1997-07-24 Weyerhaeuser Company Method for reproducing conifers by somatic embryogenesis using mixed growth hormones for embryo culture
EP0556340A4 (en) * 1990-11-02 1994-08-17 Forbio Pty Ltd Formulations of plant culture media and applications therefor
WO1993023529A2 (fr) * 1992-05-12 1993-11-25 Champagne Moët & Chandon Culture stabilisee d'agregats cellulaires et procede de developpement des embryons a partir d'une souche proembryogene destine a la regeneration de la vigne
GB2267714B (en) * 1992-06-04 1996-02-14 Unilever Plc Improvements in or relating to the cultivation of conifers
US5501972A (en) * 1992-06-10 1996-03-26 Unilever Patent Holdings B.V. Method of producing embryogenic callus of Norway spruce (Picea abies and regenerating plantlets therefrom
BR9302220A (pt) * 1993-04-23 1995-01-10 Nz Forest Research Inst Ltd Meio de crescimento para capturar e sustentar o crescimento de tecido embriogênico, processo de crescimento de tecido embriogênico, processo de capturar tecido embriogênico e processo de crescimento de tecido embriogênico pós-captura
FR2770745B1 (fr) * 1997-11-10 2001-05-18 Afocel Procede de rajeunissement de gymnospermes par embryogenese somatique
US7785884B2 (en) 2006-09-28 2010-08-31 Weyerhaeuser Nr Company Low density spreading methods for conifer somatic embryogenesis
SE531226C2 (sv) * 2006-09-28 2009-01-27 Weyerhaeuser Co Förfaranden för spridning med låg densitet för somatisk embryogenes
CN102613083A (zh) * 2012-04-08 2012-08-01 徐州工程学院 北美红杉树组织培养方法
CN113502257B (zh) * 2021-08-19 2023-08-01 内蒙古蒙荣园林绿化工程有限责任公司 一种沙地云杉体细胞胚胎萌发前的干化标记方法
CN113711918B (zh) * 2021-09-16 2022-05-13 安徽农业大学 一种黄山松的组培快繁方法

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5041382A (en) * 1989-03-09 1991-08-20 Weyerhaeuser Company High concentration enrichment of conifer embryonal cells
US5034326A (en) * 1989-10-23 1991-07-23 Weyerhaeuser Company Method for reproducing coniferous plants by somatic embryogenesis using adsorbent materials in the development stage media
US4957866A (en) * 1989-03-09 1990-09-18 Weyerhaeuser Company Method for reproducing coniferous plants by somatic embryogenesis

Also Published As

Publication number Publication date
CA2069964C (fr) 1997-12-30
WO1991005854A1 (fr) 1991-05-02
NZ235795A (en) 1993-04-28
AU672531B2 (en) 1996-10-03
AU7585294A (en) 1995-03-16
AU6641290A (en) 1991-05-16
AU654939B2 (en) 1994-12-01

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