CA2069964A1 - Method for reproducing conifers by somatic embryogenesis - Google Patents
Method for reproducing conifers by somatic embryogenesisInfo
- Publication number
- CA2069964A1 CA2069964A1 CA2069964A CA2069964A CA2069964A1 CA 2069964 A1 CA2069964 A1 CA 2069964A1 CA 2069964 A CA2069964 A CA 2069964A CA 2069964 A CA2069964 A CA 2069964A CA 2069964 A1 CA2069964 A1 CA 2069964A1
- Authority
- CA
- Canada
- Prior art keywords
- proembryos
- late stage
- abscisic acid
- somatic embryogenesis
- cultured
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 title abstract 4
- 230000030118 somatic embryogenesis Effects 0.000 title abstract 2
- 241000218631 Coniferophyta Species 0.000 title 1
- JLIDBLDQVAYHNE-YKALOCIXSA-N (+)-Abscisic acid Chemical compound OC(=O)/C=C(/C)\C=C\[C@@]1(O)C(C)=CC(=O)CC1(C)C JLIDBLDQVAYHNE-YKALOCIXSA-N 0.000 abstract 4
- FCRACOPGPMPSHN-UHFFFAOYSA-N desoxyabscisic acid Natural products OC(=O)C=C(C)C=CC1C(C)=CC(=O)CC1(C)C FCRACOPGPMPSHN-UHFFFAOYSA-N 0.000 abstract 2
- 210000002257 embryonic structure Anatomy 0.000 abstract 2
- 229930192334 Auxin Natural products 0.000 abstract 1
- 230000002745 absorbent Effects 0.000 abstract 1
- 239000002250 absorbent Substances 0.000 abstract 1
- 239000002363 auxin Substances 0.000 abstract 1
- UQHKFADEQIVWID-UHFFFAOYSA-N cytokinin Natural products C1=NC=2C(NCC=C(CO)C)=NC=NC=2N1C1CC(O)C(CO)O1 UQHKFADEQIVWID-UHFFFAOYSA-N 0.000 abstract 1
- 239000004062 cytokinin Substances 0.000 abstract 1
- 230000018109 developmental process Effects 0.000 abstract 1
- 230000035784 germination Effects 0.000 abstract 1
- 239000007788 liquid Substances 0.000 abstract 1
- 239000000463 material Substances 0.000 abstract 1
- 230000003204 osmotic effect Effects 0.000 abstract 1
- 238000004161 plant tissue culture Methods 0.000 abstract 1
- 230000002028 premature Effects 0.000 abstract 1
- 239000002689 soil Substances 0.000 abstract 1
- 230000000392 somatic effect Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/04—Plant cells or tissues
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0018—Culture media for cell or tissue culture
- C12N5/0025—Culture media for plant cell or plant tissue culture
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention is a method for reproducing coniferous trees by somatic embryogenesis using plant tissue culture. It comprises a multistage process in which a fertilized immature-embryo is excised and cultured to induce early state proembryos, which may be further cultured in liquid media without auxins or cytokinins but with the addition of abscisic acid, to develop late stage proembryos. In some species, it is desirable to significantly raise the osmotic potential of the late stage development medium. The medium may have added exogenous abscisic acid and must contain an absorbent material.
After several weeks in culture the vigor and morphology of the embryos developed by the process are improved and the tendency of premature germination is reduced. The somatic embryos may be germinated before or after storage and transplanted to soil for further growth.
After several weeks in culture the vigor and morphology of the embryos developed by the process are improved and the tendency of premature germination is reduced. The somatic embryos may be germinated before or after storage and transplanted to soil for further growth.
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US07/426,331 US5034326A (en) | 1989-10-23 | 1989-10-23 | Method for reproducing coniferous plants by somatic embryogenesis using adsorbent materials in the development stage media |
| US426,331 | 1989-10-23 | ||
| US499,151 | 1990-03-26 | ||
| US07/499,151 US5036007A (en) | 1989-03-09 | 1990-03-26 | Method for reproducing coniferous plants by somatic embryogenesis using abscisic acid and osmotic potential variation |
| PCT/US1993/007803 WO1995005070A1 (en) | 1989-03-09 | 1993-08-18 | Method for reproducing conifers by somatic embryogenesis using mixed growth hormones for embryo culture |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2069964A1 true CA2069964A1 (en) | 1991-04-24 |
| CA2069964C CA2069964C (en) | 1997-12-30 |
Family
ID=27027010
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002069964A Expired - Lifetime CA2069964C (en) | 1989-10-23 | 1990-10-22 | Method for reproducing conifers by somatic embryogenesis |
Country Status (4)
| Country | Link |
|---|---|
| AU (2) | AU654939B2 (en) |
| CA (1) | CA2069964C (en) |
| NZ (1) | NZ235795A (en) |
| WO (1) | WO1991005854A1 (en) |
Families Citing this family (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1995005070A1 (en) * | 1989-03-09 | 1995-02-23 | Weyerhaeuser Company | Method for reproducing conifers by somatic embryogenesis using mixed growth hormones for embryo culture |
| US4957866A (en) * | 1989-03-09 | 1990-09-18 | Weyerhaeuser Company | Method for reproducing coniferous plants by somatic embryogenesis |
| WO1992007460A1 (en) * | 1990-11-02 | 1992-05-14 | Forbio Pty. Ltd. | Formulations of plant culture media and applications therefor |
| EP0641383B1 (en) * | 1992-05-12 | 2002-03-13 | CHAMPAGNE MOET & CHANDON | Cellular aggregate stabilized culture and process for the development of embryos from a proembryogenic strain for use in vine regeneration techniques |
| GB2267714B (en) * | 1992-06-04 | 1996-02-14 | Unilever Plc | Improvements in or relating to the cultivation of conifers |
| US5501972A (en) * | 1992-06-10 | 1996-03-26 | Unilever Patent Holdings B.V. | Method of producing embryogenic callus of Norway spruce (Picea abies and regenerating plantlets therefrom |
| BR9302220A (en) * | 1993-04-23 | 1995-01-10 | Nz Forest Research Inst Ltd | Growth medium for capturing and sustaining embryogenic tissue growth, embryogenic tissue growth process, embryogenic tissue capture process and post-capture embryogenic tissue growth process |
| FR2770745B1 (en) * | 1997-11-10 | 2001-05-18 | Afocel | METHOD FOR THE REJUVENATION OF GYMNOSPERMS BY SOMATIC EMBRYOGENESIS |
| US7785884B2 (en) | 2006-09-28 | 2010-08-31 | Weyerhaeuser Nr Company | Low density spreading methods for conifer somatic embryogenesis |
| SE531226C2 (en) * | 2006-09-28 | 2009-01-27 | Weyerhaeuser Co | Low density spreading methods for somatic embryogenesis |
| CN102613083A (en) * | 2012-04-08 | 2012-08-01 | 徐州工程学院 | North American redwood tissue cultivation method |
| CN113502257B (en) * | 2021-08-19 | 2023-08-01 | 内蒙古蒙荣园林绿化工程有限责任公司 | Drying marking method for spruce somatic embryos before germination |
| CN113711918B (en) * | 2021-09-16 | 2022-05-13 | 安徽农业大学 | Tissue culture rapid propagation method of Chinese pine |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5034326A (en) * | 1989-10-23 | 1991-07-23 | Weyerhaeuser Company | Method for reproducing coniferous plants by somatic embryogenesis using adsorbent materials in the development stage media |
| US5041382A (en) * | 1989-03-09 | 1991-08-20 | Weyerhaeuser Company | High concentration enrichment of conifer embryonal cells |
| US4957866A (en) * | 1989-03-09 | 1990-09-18 | Weyerhaeuser Company | Method for reproducing coniferous plants by somatic embryogenesis |
-
1990
- 1990-10-22 AU AU66412/90A patent/AU654939B2/en not_active Expired
- 1990-10-22 CA CA002069964A patent/CA2069964C/en not_active Expired - Lifetime
- 1990-10-22 WO PCT/US1990/006057 patent/WO1991005854A1/en active Application Filing
- 1990-10-23 NZ NZ23579590A patent/NZ235795A/en unknown
-
1994
- 1994-10-14 AU AU75852/94A patent/AU672531B2/en not_active Expired
Also Published As
| Publication number | Publication date |
|---|---|
| AU6641290A (en) | 1991-05-16 |
| WO1991005854A1 (en) | 1991-05-02 |
| AU654939B2 (en) | 1994-12-01 |
| AU672531B2 (en) | 1996-10-03 |
| CA2069964C (en) | 1997-12-30 |
| AU7585294A (en) | 1995-03-16 |
| NZ235795A (en) | 1993-04-28 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request | ||
| MKEX | Expiry |