CA2060314A1 - Agarose a faible electro-osmose - Google Patents

Agarose a faible electro-osmose

Info

Publication number
CA2060314A1
CA2060314A1 CA2060314A CA2060314A CA2060314A1 CA 2060314 A1 CA2060314 A1 CA 2060314A1 CA 2060314 A CA2060314 A CA 2060314A CA 2060314 A CA2060314 A CA 2060314A CA 2060314 A1 CA2060314 A1 CA 2060314A1
Authority
CA
Canada
Prior art keywords
agarose
less
concentration
purified
content
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA2060314A
Other languages
English (en)
Other versions
CA2060314C (fr
Inventor
Francis H. Kirkpatrick
Kenneth Guiseley
Richard Provonchee
Samuel Nochumson
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
FMC Corp
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2060314A1 publication Critical patent/CA2060314A1/fr
Application granted granted Critical
Publication of CA2060314C publication Critical patent/CA2060314C/fr
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D57/00—Separation, other than separation of solids, not fully covered by a single other group or subclass, e.g. B03C
    • B01D57/02—Separation, other than separation of solids, not fully covered by a single other group or subclass, e.g. B03C by electrophoresis
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/24—Extraction; Separation; Purification by electrochemical means
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/24—Extraction; Separation; Purification by electrochemical means
    • C07K1/26—Electrophoresis
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
    • C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
    • C08B37/0006—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid
    • C08B37/0036—Galactans; Derivatives thereof
    • C08B37/0039—Agar; Agarose, i.e. D-galactose, 3,6-anhydro-D-galactose, methylated, sulfated, e.g. from the red algae Gelidium and Gracilaria; Agaropectin; Derivatives thereof, e.g. Sepharose, i.e. crosslinked agarose
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44752—Controlling the zeta potential, e.g. by wall coatings
CA002060314A 1989-08-03 1990-07-31 Agarose a faible electro-osmose Expired - Fee Related CA2060314C (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US389,141 1989-08-03
US07/389,141 US4983268A (en) 1989-08-03 1989-08-03 High gel strength low electroendosmosis agarose

Publications (2)

Publication Number Publication Date
CA2060314A1 true CA2060314A1 (fr) 1991-02-04
CA2060314C CA2060314C (fr) 1994-09-06

Family

ID=23537006

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002060314A Expired - Fee Related CA2060314C (fr) 1989-08-03 1990-07-31 Agarose a faible electro-osmose

Country Status (5)

Country Link
US (1) US4983268A (fr)
EP (1) EP0485510A4 (fr)
JP (1) JPH087187B2 (fr)
CA (1) CA2060314C (fr)
WO (1) WO1991001995A1 (fr)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2067429T3 (es) * 1989-01-13 1996-12-16 Fmc Corp Geles de resolucion de polisacaridos y sistemas de geles para electroforesis en apilamiento.
AU8075991A (en) * 1990-06-13 1992-01-23 Fmc Corporation Derivatized agarose products and processes therefor
EP0464299B1 (fr) * 1990-07-04 1995-01-11 Marcin Krotkiewski Préparation anti-hypertensive
US5230832A (en) * 1991-01-24 1993-07-27 Brandeis University Galactomannan-agarose binary gel for nucleic acid electrophoresis
US5455344A (en) * 1993-09-03 1995-10-03 Fmc Corporation Agarose compositions for nucleic acid sequencing
FR2722295B1 (fr) * 1994-07-07 1996-10-04 Roussy Inst Gustave Methode d'analyse d'adn dite sscp et gel d'electro-phorene
US8071133B2 (en) * 2001-08-20 2011-12-06 Stiefel Laboratories, Inc. Oral dosage forms of water insoluble drugs and methods of making the same
US20040115266A1 (en) * 2002-02-01 2004-06-17 Namburi Ranga Raju Oral itraconazole formulations and methods of making the same
US20050267296A1 (en) * 2004-06-01 2005-12-01 Council Of Scientific & Industrial Research Cost-effective process for preparing agarose from gracilaria spp.
EP1928249B1 (fr) * 2005-09-26 2012-02-29 The Rogosin Institute, Inc. Macro-perles contenant des cellules sécrétoires comprenant de l'agarose seakem gold et leurs utilisations
WO2010107503A1 (fr) 2009-03-19 2010-09-23 Millipore Corporation Élimination de micro-organismes dans des échantillons de fluide en utilisant des milieux de filtration à nanofibres
US9623352B2 (en) 2010-08-10 2017-04-18 Emd Millipore Corporation Method for retrovirus removal
EP2694196B1 (fr) 2011-04-01 2021-07-21 EMD Millipore Corporation Nanofibre contenant des structures composites
EP3283202A1 (fr) 2015-04-17 2018-02-21 EMD Millipore Corporation Procédé de purification d'une matière biologique d'intérêt dans un échantillon au moyen de membranes d'ultrafiltration à nanofibres mises en oeuvre en mode de filtration de flux tangentiel
CN108835612A (zh) * 2018-06-20 2018-11-20 罗国球 一种高温低浓度碱制制取琼脂的方法
US11691122B2 (en) 2018-11-02 2023-07-04 Advanced Aesthetic Technologies, Inc. Irradiated agarose, compositions thereof, and related methods
AU2019371245A1 (en) * 2018-11-02 2021-06-03 Advanced Aesthetic Technologies, Inc. Irradiated agarose, compositions thereof, and related methods
CN110698573B (zh) * 2019-11-19 2021-07-30 中国科学院海洋研究所 一种高品质琼脂糖的制备方法
CN115260341B (zh) * 2022-07-29 2023-05-02 国药集团化学试剂有限公司 一种高质量琼脂糖及其制备方法

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB639222A (en) * 1941-06-15 1950-06-21 Tjoa Sie Lian Process for purifying agar-agar
US3753972A (en) * 1970-04-28 1973-08-21 Research Corp Fractionation of agar
US4290911A (en) * 1979-02-07 1981-09-22 Fmc Corporation Agarose composition, aqueous gel and method of making same
CA1206666A (fr) * 1982-02-19 1986-06-24 Samuel Nochumson Copolymere d'acrylamide et de resine polysaccharidique utilise comme milieu pour l'electrophorese sur gel
US4990611A (en) * 1987-08-17 1991-02-05 Fmc Corporation Agarose purification method using glycol

Also Published As

Publication number Publication date
JPH04503328A (ja) 1992-06-18
US4983268A (en) 1991-01-08
EP0485510A4 (en) 1992-11-04
EP0485510A1 (fr) 1992-05-20
JPH087187B2 (ja) 1996-01-29
CA2060314C (fr) 1994-09-06
WO1991001995A1 (fr) 1991-02-21

Similar Documents

Publication Publication Date Title
CA2060314A1 (fr) Agarose a faible electro-osmose
SE8600423D0 (sv) Forfarande for rening av proteiner fran mjolk och fran dess derivat
EP0921818A4 (fr) Procedes et compositions therapeutiques et diagnostiques obtenues a partir de proteines jagged/notch et d'acides nucleiques
ES8607727A1 (es) Procedimiento para la estabilizacion de preparados farmaceuticos en forma de suspensiones conteniendo sucralfato
WO1990007978A1 (fr) Gels de resolution de polysaccharide et systemes de gel pour electrophorese par empilement
DE3781031D1 (de) Markierung von traegermolekuelen mit metallionen.
EP0356187A3 (fr) Electrophorèse utilisant des champs électriques à contour imposé
IL79972A (en) Protein recovery from inclusion bodies produced in transformant microorganisms
AU7067287A (en) Method and apparatus for separating proteins
AU7918991A (en) Method of treating bladder cancer cells
ES2105033T3 (es) Metodo de purificacion de proteinas.
DE3784728T2 (de) Reinigung von rekombinantem interleukin-1.
DE60121999D1 (de) Verfahren zur herstellung von zusammensetzungen mit geringer salzkonzentration
GB1479851A (en) Chemical process
ES8200560A1 (es) Un procedimiento para producir interferon de fibroblasto hu-mano
GR852622B (fr)
NL7017514A (en) Purification of proteins
Wu et al. Discontinuous agarose electrophoretic system for the recovery of stained proteins from polyacrylamide gels
DE3788170T2 (de) Methode zur Reinigung und Isolierung von zwei ionischen Formen von Somatomedin.
ATE272072T1 (de) Verfahren zur reinigung von menschlichem interleukin-1 rezeptor antagonist aus rekombinanten e.coli
AU1239495A (en) An electrophoretic method to assess human cerebrospinal fluid
DE69427654T2 (de) Komformationelle trennung von nukleinsäuren mit hilfe von hochmodifizierter agarose
FR2552762B1 (fr) Nouveaux amides piperaziniques et homopiperaziniques derives de l'acide 3,4-dioxymethylene cinnamique, leur procede de preparation et leur application en therapeutique
KR910015699A (ko) 리포코틴의 정제방법
KR970006500A (ko) 재조합 효모로부터 발현된 감마 인터페론의 정제 방법

Legal Events

Date Code Title Description
EEER Examination request
MKLA Lapsed
MKLA Lapsed

Effective date: 19990803