CA1339620C - The preparation of an ester of penicilling. - Google Patents

The preparation of an ester of penicilling.

Info

Publication number
CA1339620C
CA1339620C CA000615880A CA615880A CA1339620C CA 1339620 C CA1339620 C CA 1339620C CA 000615880 A CA000615880 A CA 000615880A CA 615880 A CA615880 A CA 615880A CA 1339620 C CA1339620 C CA 1339620C
Authority
CA
Canada
Prior art keywords
penicillin
reaction
preparation
ester
bromide
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CA000615880A
Other languages
French (fr)
Inventor
Derek Reginald Palmer
Robert Graham Tyson
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Astra Lakemedel AB
Original Assignee
Astra Lakemedel AB
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB838300331A external-priority patent/GB8300331D0/en
Priority claimed from CA000431361A external-priority patent/CA1283120C/en
Application filed by Astra Lakemedel AB filed Critical Astra Lakemedel AB
Application granted granted Critical
Publication of CA1339620C publication Critical patent/CA1339620C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Landscapes

  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

Improved methods for the preparation of 1-ethoxycarbonyloxyethyl ester of penicillin-G in which alphabromodiethylcarbonate is used as a reactant.

Description

1339~20 The present invention, which is divided out of Application Serial No. 431,361, relates to the use of ~-bromodi-ethylcarbonate in the preparation of the l-ethoxycarbonyloxyethyl ester of penicillin-G.
Bacampicillin, or the l-ethoxycarbonyloxyethyl ester of the 6-(D-(-)-~-phenylacetamido)penicillanic acid of formula CH - CO - NH - CH - CH C
CH3 (I) is an amplicillin ester which is extremely important from the thera-peutic point of view since it is well absorbed when administered orally and gives much higher blood levels than ampicillin.
This ester is isolated in the form of a hydrochloride.
On the basis of previous known processes (cf. Belgian patent No. 772723), bacampicillin hydrochloride can be synthesized by the two following methods.
A) Reaction of potassium benzylpenicillin with ~-chlorodiethylcarbonate in an aqueous solution of 70% dioxane in the presence of sodium bicarbonate. The l-ethoxycarbonyloxyethyl ester of benzylpenicillin which is obtained is subjected to the reaction of removing the phenylacetic chain, via the iminochloride-iminoether, in order to obtain the l-ethoxycarbonyloxyethyl ester of the 6-aminopenicillanic acid, which is isolated as the hydro-chloride.
By subsequent condensation of the latter intermediate -- 1 -- *

-- 1339~20 with D-(-)-~-phenylglycine, the compound according to formula I
is obtained.
B) Esterification reaction of the 6-(D-(-)-~azido-~-phenylacetamido) penicillanic acid with ~-chlorodiethylcarbonate in a polar solvent.
Subsequently, by catalytic hydrogenation of the 1-ethoxycarbonyloxyethyl ester of the 6-(D-(-)-~-azido-~-phenyl-acetamido) penicillanic acid the compound according to formula I
is obtained.
As one can see, these methods are rather complex since they involve the use of numerous raw materials and lengthy processing times.
The invention An object of this invention is to provide a method of preparing the active substance concerned which is easier to carry out and industrially more advantageous.
The invention of Parent Patent Application 431,361 provides the novel compound ~-bromodiethylcarbonate. The invention of the present divisional application provides for the use of ~-bromodiethylcarbonate in the preparation of the ethoxycarbonyloxy-ethyl ester of penicillin-G.
~ -Bromodiethylcarbonate is used with great advantage as a reactant in these esterification processes. The use of ~-bromodiethylcarbonate leads to particularly high yield and high purity of the final products such as bacampicillin.
Application Serial No. 431,361 is concerned with 133~621~

improvements in and relating to the preparation of ~-bromodiethyl-carbonate of the formula:

CH3 - 1H.O.CO.O.C2H5 (II) This compound is sometimes referred to by the alter-native name ethyl-o~bromoethylcarbonate. The alpha-bromo-diethyl-carbonate of the formula (II) may be used in the preparation of the ethoxycarbonyloxyethyl ester of penicillin-G.
Application Serial No. 431,361 discloses two novel and inventive processes, herebelow denoted process A and process B, for the preparation of alpha-bromodiethylcarbonate of the formula II.
A. The first of these processes, process A, comprises the steps of :
(a) reacting an aldehyde of the formula CH3CHO (III) with carbonyl bromide COBr2 (IV) to give an alpha-bromoethyl-bromoformate of the formula:
Br CH3 CH.O.CO.Br (V) and;
(b) reacting the alpha-bromoethyl-bromoformate of formula V with an alcohol of the formula C2H5-OH to yield the desired alpha-bromo-diethylcarbonate of the formula II.
Thus, the process A may be summarised by the reaction scheme:

1~39~20 Br +C2H5OH Br CH3CHO + COBr~ CH3- CH.O.CO.Br ' CH3 _ CH.O.CO.OC2H5 + HBr The alpha-bromoethyl-bromoformate of the formula V
is in itself a new compound.
The reaction between the aldehyde, CH3CHO, and car-bonyl-bromide is most suitably carried out in the presence of a catalyst which may be, for example, a tertiary amine (for example a tertiary aliphatic amine, a tertiary mixed alkyl/aryl amine or a tertiary aromatic amine), tertiary phosphine, amide, substituted urea or thiourea, phosphoric acid amide, tertiary oxonium or sul-phonium salt, or a quaternary ammonium or phosphonium salt.
Preferred examples of catalysts for use in the process A according to the invention include pyridine, dimethylformamide, tetra-n-butyl urea, hexamethyl-phosphoric-tri-amide and benzyltrimethyl ammonium bromide.
The catalyst is suitably used in an amount of from 0.05 to 0.5, preferably from 0.05 to 0.15, moles of catalyst per mole of aldehyde.
The reaction between the aldehyde and the carbonyl bromide is suitably carried out in the presence of a solvent which may be,for example, an aromatic hydrocarbon such as toluene or a halogenated hydrocarbon such as dichloromethane, carbon tetra-chloride or chlorobenzene. The reaction between the aldehyde and the carbonyl bromide is suitably carried at a temperature of from -40 to 120~C, preferably 0 - 40~C. The carbonyl bromide will usually be used in molar excess with respect to the aldehyde, 133~620 suitably in a molar excess of from 10 to 100%, preferably from 20 to 50~-The intermediate alpha-bromoethyl-bromoformate of formula V produced in step (a) of the process A need not be iso-lated prior to reaction with the alcohol C2H5OH and, indeed, it is generally preferred not to do so. Thus, in accordance with a preferred embodiment, the reaction mixture obtained from step (a) is freed of excess carbonyl bromide, for example by warming under reduced pressure or by purging with nitrogen. The crude alpha-bromoethyl-bromoformate-containing reaction mixture is then reacted with an excess of the alcohol. The reaction may conven-iently be effected by heating the mixture under reflux until the evolution of hydrogen bromide ceases or by adding a tertiary base to the mixture and, if necessary, warming it. Any residual catalyst from step (a) or its complex with carbonyl bromide does not appear to interfere with the subsequent reaction and, in some cases, appears beneficial.
The resultant crude alpha-bromocarbonate may con-veniently be isolated from the reaction mixture by fractional distillation under reduced pressure.
Process A is illustrated in Examples 1 and 2 r which are given by way of illustration only.
B. The second process, process B, for the prepara-tion of ~-bromodiethylcarbonate will now be described. Method B
is exemplified in Example 3, which is given by way of illustration only.

l~C.~62a, Process B is concerned with improvements in and relating to the preparation of ~bromodiethylcarbonate by a modification of the Finkelstein reaction, that is by reaction of an alkyl chloride or arylalkyl chloride (or a compound contain-ing such a group) with an alkali metal bromide or alkali metal iodide to replace the chloride substituent by a bromine or iodine substituent respectively; or by the reaction of an alkyl bromide or arylalkyl bromide (or a compound containing such a group) with an alkali metal iodide to replace the bromine substituent by an iodine substituent.
The Finkelstein reaction is useful since the result-ing iodides are generally more reactive than the bromides which in turn are more reactive than the chlorides. In some cases only catalytic amounts of the alkali metal bromide or iodide are necessary and the resulting more reactive species is allowed to react with the desired substrate regenerating the alkali metal bro-mide or iodide, thus continuing the reaction.
Not all optionally substituted alkyl chlorides or arylalkyl chlorides undergo the reaction and, in particular, it has been found difficult to carry out the reaction with alpha-chloro esters and alpha-chlorocarbonates, that is compounds in which the chlorine atom is attached to a carbon atom which is, in turn, attached to either end of a group -C(0)-0-. An example of such an alpha-chloro-carbonate is ~-chlorodiethylcarbonate, which is a known intermediate in the preparation of ethoxycarbonyloxyethyl esters of 6-aminopenicillanic acid and of penicillins as described above.

133g~20 It has now been found that this problem may be overcome by carrying out the reaction using a two-phase solvent system, one phase of which is water and the other is a water-immiscible organic solvent, in the presence of a phase transfer catalyst.
According to process B therefore, there is provided a process for the preparation of ~-bromodiethylcarbonate by reaction of ~-chlorodiethylcarbonate with an alkali metal bromide, which process is characterized in that the reaction is carried out in a two-phase solvent system comprising water and a water-immiscible organic solvent in the presence of a phase transfer catalyst.
Suitable water-immiscible organic solvents include halogenated hydrocarbons, for example halogenated paraffins such as dichloromethane; and aromatic hydrocarbons such as toluene.
Suitable phase transfer catalysts include quaternary ammonium salts, for example tetraalkyl ammonium salts such as cetyltrimethyl ammonium bromide and tetra-n-butyl ammonium hydrogen sulphate.
The alkali metal bromide may, for example, be sodium, potassium, or lithium bromide, lithium bromide being preferred.
Thus, in process B, ~-chlorodiethylcarbonate of the formula:
Cl O
..
CH3 - CH - O - C - O - C2H5 (VI) is reacted in a two-phase solvent system, one phase of which is water and the other is a water-immiscible organic solvent, with an alkali metal bromide of the formula 1333~20 R-Br (VII) in which formula R is an alkali metal such as Na, K and Li, to the formation of the compound of the formula:
Br O
..
CH - CH - O - C ~ ~ C2 5 (VIII) As noted above, the preferred alkali metal R is Li so that LiBr is a preferred reagent of the formula VII.
In connection with process B it has been found that lithium bromide may be used with advantage in a conventional Finkelstein reaction (i.e. one employing a single phase organic solvent system), for example to halogenate an alpha-chloro-carbonate. This method is exemplified in Example 4.
Accordingly, Application Serial No. 431,361 discloses, in accordance with a further embodiment thereof, a process for the preparation of ~-bromodiethylcarbonate which comprises reacting ~-chlorodiethylcarbonate with lithium bromide.
Suitable solvents for such a process include lower aliphatic alcohols, lower aliphatic ketones, lower aliphatic ethers and lower aliphatic amides of formic acid.
The invention of this relates to the use of the novel compound ~-bromodiethylcarbonate in the preparation of the ethoxycarbonyloxyethyl ester of penicillin-G.
Accordingly, the present invention provides a process for the preparation of an ethoxycarbonyloxyethyl ester of penicillin-G which comprises reacting penicillin-G or a salt thereof with a ~-bromodiethylcarbonate compound of the formula Br\
~ CH-O-CO-O-C2H5 and recovering the desired product.
The reaction is carried out in the presence of a quaternary ammonium compound as catalyst. Suitably the said quaternary ammonium compound is present in an amount of 1-25, preferably 1-10% of the equimolar amount with respect to the amount of penicillin-G.
The ethoxycarbonyloxyethyl ester of penicillin-G is used as is known in the art in the preparation of any desired such semisynthetic penicillin ester by acylating the 6-NH2 group after removing the side chain in e.g. the penicillin-G ester obtained.
This aspect of the invention is concerned with improvements in and relating to the preparation of esters by the reaction of salts of carboxylic acids with a-bromodiethyl-carbonate.
The reaction of metal salts of carboxylic acids with alkyl halides of arylalkyl halides to form esters is well known.
However, yields are not particularly high and the reaction generally requires forcing conditions such as high temperatures and/or extended reaction times. These forcing conditions limit the synthetic utility of the reaction and its commercial applica-bility to heat sensitive and labile substances such as pyrethroids, prostaglandins, peptides, penicillins and cephalosporins.
The British Patent Specification 1443738 discloses A' n the use of a quaternary ammonium salt of penicillins and cefalo-sporins in place of a metal salt thereof in the preparation of esters of penicillins and cefalosporins.
The preparation of the quaternary ammonium salt of the acid may be time-consuming and expensive. However, as is also disclosed in the British patent specification 1443738, it is not necessary to first prepare the quaternary ammonium salt of a penicillin or cefalosporin, but the reaction may be carried out by reacting a metal salt of the carboxylic acid, that is the 6-apa, penicillin or cefalosporin with the alkyl or arylalkyl halide in the presence of a quaternary ammonium salt, other than the salt of the carboxylic acid.

It has now been found, according to the present inven-tion, that it is not necessary to employ the said quaternary ammonium salt in a stoichiometric amount with respect to the car-boxylic acid, that is the penicillin-G but that a less than stoichiometric amount with respect to the carboxylic acid penicillin-G will be sufficient.
According to the invention, therefore, there is provided a process for the preparation of an ethoxycarbonyloxy-ethyl ester of penicillin-G by reaction of a metal salt of the penicillin-G or cefalosporin with ~-bromodiethylcarbonate in the presence of a quaternary ammonium salt (other than a salt of the said carboxylic acid) whereby the quaternary ammonium compound is present in a less than stoichiometric amount with respect to the penicillin-G.

133~9620 In accordance with a preferred embodiment of the invention, between 1~ and 25% of an equivalent of the quaternary ammonium salt is used for each equivalent of the metal salt of the carboxylic acid, and more preferably between 1% and 10% of an equivalent of the quaternary ammonium salt is used.
The quaternary ammonium salt of the carboxylic acid is suitably prepared by reaction of a metal salt of the carboxylic acid with a quaternary ammonium salt of an acid other than said carboxylic acid, typically a mineral acid such as hydrochloric, hydrobromic or sulphuric acid.
Suitable metal salts of carboxylic acids for use in accordance with the present aspect of the invention (either as precursors for the carboxylic acid quaternary ammonium salt or as such) are alkali metal or alkaline earth salts such as sodium, potassium, lithium, magnesium and calcium salts. Suitable quater-nary ammonium salts of acids other than the carboxylic acid (for use either as precursors for the carboxylic acids quaternary ammon-ium salts or as such) include for example tetra-alkyl ammonium salts such as tetra-n-butyl ammonium bromide and cetyltrimethyl ammonium bromide and quaternary pyridinium salts such as cetyl-pyridinium bromide. Suitable halides include fluorides, chlorides, bromides and iodides, preferably activated fluorides or activated chlorides or bromides or iodides.
The esterification reaction in accordance with this aspect of the invention may be carried out in the presence or absence of a solvent. Suitable solvents include lower aliphatic 9 6 2 1~

alcohols, lower aliphatic ketones, lower aliphatic amides of formic acid and dimethylsulphoxide. Alternatively, when no solvent is used, an excess of the ester forming halide may be used, particularly if this is a liquid at the temperature of the reaction.
In the previously described aspect of the inven-tion which relates to the use of ~-bromodiethylcarbonate in the preparation of the ethoxycarbonyloxyethyl ester of penicillin-G, the use of catalyst is optional. Approximately equimolar amounts of the quaternary ammonium salt of the carboxylic acid and the ester forming halide may be used in the reaction.
Preferably between 5% and 100% excess of the ester forming halide is used for each equivalent of the salt of the carboxy-lic acid used and more preferably an excess of between 20%
and 60% of the ester forming halide is used.
The improvements in the esterification processes of the invention are particularly suitable for the preparation of the ester of penicillin-G and thus, in accordance with the invention penicillin-G of the formula:

~ CH2CO-NH CH3 XI

/~N
~ CO2H

or a salt thereof, is reacted with a compound of the formula Br in the presence of a catalyst.
In accordance with a preferred embodiment of the lnvention the catalyst is a quaternary ammonium salt, preferably tetra-n-butylammonium bromide.
In order that the invention may be well understood the following examples are given by way of illustration.~-Examples 1 to 5 illustrate the preparation of the novel compound ethyl ~-bromoethylcarbonate by methods disclosed in Application Serial No.
431,361.
Example 1 A mixture of acetaldehyde (44 g, 1 mole), carbon tetrachloride (300 ml) and freshly distilled carbonyl bromide (235 g, 1.25 mole) was cooled to 0~C and maintained at this tempera-ture by external cooling during the addition over a period of 1 hour of pyridine (11.9 g, 0.15 mole).
The mixture was allowed to warm up to ambient tempera-ture and then heated to 50~C and maintained at this temperature for a period of 3 hours during which time a precipitate formed.
Evaporation of the reaction mixture under reduced pressure at 50~C gave a semi solid oily mass which readily dissolv-ed in ethanol (92 g, 2 mole) on warming and heating under reflux.
After heating under reflux for a further 2 hours, excess ethanol 1~39~;20 was removed in vacuo and the residue triturated with water (100 ml) and methylene dichloride (200 ml~.
Separation of the organic layer and fractional dis-tillation afforded pure ethyl alpha-bromo-ethyl-carbonate (130 g, 66~ yield) having a boiling point of 90-92~C at 45 mms of mercury pressure and identical in all respects with an authentic specimen.
Example 2 A mixture of acetaldehyde (44 g, 1 mole), dichloro-methane (300 ml) and hexamethylphosphoric-tri-amide (17.9 g, 0.1 mole) was cooled to -10~C and freshly distilled carbonyl bromide (207 g, 1.1 mole) was gradually added over a period of 4 hours during which time the temperature was allowed to rise to 10~C.
The mixture was then heated under gentle reflux (ca. 40~C) for 4 hours. While still under reflux, ethanol (69 g, 1.5 mole) was carefully added over a period of 1 hour and heating under reflux continued for a further 1 hour.
Fractional distillation of the resulting mixture afforded pure ethyl alpha-bromoethyl-carbonate directly (114 g, 58% yield).
The authenticity of the ethyl alpha-bromoethyl-carbonate formed was confirmed by analysis and independent synthe-sis as follows.
Diethylcarbonate (118 g, 1.0 mole) was stirred and heated to between 110~C and 120~C and illuminated by a 150 watt tungsten filament lamp. Bromine (96 g, 0.6 mole) was added drop-wise over a period of 3 to 4 hours and at such a rate that the ~- 1339~20 mixture did not deepen beyond a pale orange colour.
After addition of bromine was complete, the mixture was cooled to ambient temperature and sodium bicarbonate (20 g) added.
Distillation and fractionation of the resulting mixture gave authentic ethyl alpha-bromoethyl carbonate (84.2 g, 70% yield) having a boiling point of 87-88~C at 40 mms of mercury pressure.
Example 3 A mixture of lithium bromide (43 g, 0.5 m), ethyl alphachloroethylcarbonate (15.3 g, 0.1 m); water (100 ml), dichloromethane (100 ml) and cetyl trimethyl ammonium bromide (1.5 g) was stirred at ambient temperature for 24 hours. The aqueous layer was removed and replaced by a fresh solution of lithium bromide (26 g, 0.3 m) in water (40 ml) containing cetyl trimethyl ammonium bromide (1 g). After stirring for a further 24 hours during which time the temperature was raised to 35~C, the organic layer was separated, dried and vacuum distilled to afford after repeated fractionation the new compound, ethyl alpha-bromoethylcarbonate (15.0 g, 76% yield) having a boiling point of 90-92~C at 35 mms of mercury pressure.
Found: C 30.7 H 4.8 Br 40.1%
Calculated: C 30.5 H 4.6 Br 40.6 The NMR spectrum exhibited peaks as follows:-1339~20 1.2 - 1.6 (3H, triplet) CH2.CH3 2.0 - 2.2 (3H, doublet) -CH.CH3 4.1 - 4.5 (2H, Quartet) CH2.CH3 6.5 - 6.8 (lH, Quartet) -CH.CH3 Example 4 Lithium bromide (17.4 g, 0.2 m) was dissolved in di-methyl formamide (150 ml) and the mixture cooled to ambient tem-perature. Ethyl alpha-chloroethyl carbonate (30.5 g, 0.2 m) was added and the mixture stirred at ambient temperature for 24 hours.
The precipitated lithium chloride was filtered off and the filtrate vacuum distilled to afford after careful re-fractionation, ethyl alpha-bromoetpyl carbonate in 76~ yield based upon recovered ethyl alpha-chloroethyl carbonate.
Example 5 The authenticity of the foregoing new compound ethyl alpha-bromoethyl carbonate was confirmed by independent synthesis as follows:-A mixture of diethyl carbonate (35 g, 0.3 m) in carbon tetrachloride (50 ml) and alpha-azo-isobutyronitrile (AIBN) (0.1 g) was heated to gentle reflux and dibromodimethyl hydantoin (28.6 g, 0.1 m) was added in small aliquots over a period of 8 hours together with further additions of AIBN (8xO.05 g): care being taken to ensure that free bromine did not accumulate in the re-action mixture. At the end of the reaction the mixture was subjec-ted to vacuum fractional distillation to afford pure ethyl alpha-bromoethyl carbonate (32.3 g, 82% yield) identical in all respects -- 1~ --13.~9~20 with the product of Examples 3 and 4.
Example 6 Benzylpenicillin ethoxycarbonyloxyethyl ester A mixture of potassium penicillin-~ (7.4 g, 20 mmole), ethyl alpha-chloroethyl carbonate (4.6 g, 30 mmole), tetra-n-butyl ammonium bromide (0.8 g, 2.5 mmole) and acetone (80 ml) were stirred and heated under gentle reflux for 4 hours. Excess acetone was removed under partial vacuum and the residue triturated with ice-cold water and methyl isobutylketone. Evaporation of the dried methyl isobutylketone under vacuum gave a semi-crystalline oil (3.8 g) which on trituration with ethanol deposited white crystals (0.9 g) of the alpha-(ethoxycarbonyloxy)-ethyl ester of penicillin-G having a purity of 98-g9% by HPLC.
Found: C 43.0 H 7.4 N 7.7%
Calculated: C 43.4 H 7.4 N 8.0%
Example 7 Benzylpenicillin ethoxycarbonyloxyethyl ester The foregoing experiment of Example 11 was repeated using ethyl alpha-bromoethyl carbonate (5.9 g, 30 mmole) instead of ethyl alpha-chloroethyl carbonate, whereon there was obtained, on evaporation of the methyl isobutyl ketone, 6.0 g of a semi-crystalline oil. Trituration of this oil with warm ethanol and then cooling afforded white crystals (2.5 g, 35% yield) of the alpha-(ethoxycarbonyloxy)-ethyl ester of penicillin-G.
Example 8 Benzylpenicillin ethoxycarbonyloxyethyl ester Potassium benzylpenicillanate (25.08 g, 66.7 mmol) sodium bicarbonate (0.50 g, 6.0 ~mol), and tetrabutylammonium bromide (2.15 g, 6.67 mmol) were carefully stirred in methylene 133962~

chloride (41ml) and warmed to 40~C. When this temperature was reached ~-bromodiethyl carbonate 17.16 g, 86.7 mmol) was added and the slurry was stirred for 4.0 hours. Water (30 ml~ was added, followed by a mineral acid to a pH of approx. 5. The mixture was stirred for approx. 4 hours, during which time sodium hydroxide (4%) was added in order to maintain pH between 2.5-3Ø Methylene chloride (50 ml) was then added and the mixture was allowed to separate for a few minutes. The organic phase was washed with water (65 ml) and was then evaporated under reduced pressure. The oily product thus obtained was dissolved in methylene chloride (100 ml) and was evaporated again. The remaining oil was dissolved in methylene chloride to a total volume of 100 ml.
HPLC-analysis of the methylene chloride solution showed a yield of benzylpenicillin ethoxycarbonyloxyethyl ester of 96-97%.
Example 9 Benzylpenicillin ethoxycarbonyloxyethyl ester Potassium benzylpenicillinate (5.02 g, 13.3 mmol) and potassium bicarbonate (2.99 g, 38.3 mmol) in dimethyl sulfoxide (13.5 ml) were carefully stirred in an ice-bath. ~-Bromodiethyl carbonate (3.70 g, 18.6 mmol) was added over a period of 30-40 min.
using a syringe pump. Stirring was continued while keeping the reaction mixture in the ice-bath. HPLC-analyses showed that a yield of about 70% of the benzylpenicillin ethoxycarbonyloxyethyl ester was obtained within 5-10 min.
Example 10 Benzylpenicillin ethoxycarbonyloxyethyl ester Potassium benzylpenicillinate (47.03 g, 125 mmol) 1~39620 sodium bicarbonate (0.94 g, 11 mmol), and tetrabutylammoniu~, bro-mide (2.01 g, 6.25 mmol) were carefully stirred in acetone ~77 ml) and warmed to 40~C. When this temperature was reached ~-bromo-diethyl carbonate (26.06 g, 131 mmol) was added and the slurry was stirred for 4.5 hours. Water (56 ml) was added, followed by a mineral acid to a pH of approx. 5. The mixture was stirred for approx. 3 hours, during which time sodium hydroxide (4%) was added in order to maintain pH between 4.5-4.8. Butyl acetate (100 ml) was then added and the mixture was allowed to separate for a few minutes. The organic phase was washed with water (80 ml) and then evaporated under reduced pressure. The rem~ining oily product was dissolved in methylene chloride to a total volume of 250 ml.
HPLC-analysis of the methylene chloride solution showed a yield of benzylpenicillin ethoxycarbonyloxyethyl ester of 9~-99%.

19 ~

Claims (8)

1. A process for the preparation of an ethoxycarbonyloxyethyl ester of penicillin-G, which comprises reacting penicillin-G
or a salt thereof with an a-bromodiethylcarbonate compound of the formula in the presence of a quaternary ammonium salt as catalyst and recovering the desired product.
2. A process according to claim 1 wherein potassium penicillin-G is reacted with the a-bromodiethylcarbonate.
3. A process according to claim 1 or 2 wherein the reaction is carried out in the presence of tetra-n-butylammonium bromide as catalyst.
4. A process according to claim 1 for the preparation of benzylpenicillin ethoxycarbonyloxyethyl ester which comprises reacting together potassium penicillin-G, a-bromodiethylcarbonate and tetra-n-butylammonium bromide in a solvent, and recovering the desired product.
5. A process according to claim 3 wherein the amount of catalyst is from 1 to 25% of the equimolar amount with respect to penicillin-G.
6. A process according to claim 4 wherein the amount of catalyst is from 1 to 25% of the equimolar amount with respect to penicillin-G.
7. A process according to claim 3 wherein the amount of catalyst is from 1 to 10% of the equimolar amount with respect to penicillin-G.
8. A process according to claim 4 wherein the amount of catalyst is from 1 to 10% of the equimolar amount with respect to penicillin-G.
CA000615880A 1982-09-20 1990-10-02 The preparation of an ester of penicilling. Expired - Fee Related CA1339620C (en)

Applications Claiming Priority (9)

Application Number Priority Date Filing Date Title
GB8226751 1982-09-20
GB8226751 1982-09-20
GB8228622 1982-10-06
GB8228622 1982-10-06
GB8232629 1982-11-16
GB8232629 1982-11-16
GB838300331A GB8300331D0 (en) 1983-01-07 1983-01-07 Preparation of bromo carbonates
GB8300331 1983-01-07
CA000431361A CA1283120C (en) 1982-09-20 1983-06-28 Intermediates and improvements in the preparation of antibiotics

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
CA000431361A Division CA1283120C (en) 1982-09-20 1983-06-28 Intermediates and improvements in the preparation of antibiotics

Related Child Applications (1)

Application Number Title Priority Date Filing Date
CA000616884A Division CA1339602C (en) 1988-09-06 1994-06-23 A syringe tip adaptor

Publications (1)

Publication Number Publication Date
CA1339620C true CA1339620C (en) 1998-01-06

Family

ID=27508222

Family Applications (1)

Application Number Title Priority Date Filing Date
CA000615880A Expired - Fee Related CA1339620C (en) 1982-09-20 1990-10-02 The preparation of an ester of penicilling.

Country Status (1)

Country Link
CA (1) CA1339620C (en)

Similar Documents

Publication Publication Date Title
CA1236450A (en) Synthesis route for bacampicillin
US4606865A (en) Methods for the preparation of α-bromodiethylcarbonate
CA1339620C (en) The preparation of an ester of penicilling.
JPH0764789B2 (en) Manufacturing method of methacrylic acid ester
JPH05112526A (en) Process for manufacturing 3-substituted 4-cyanopyrrole compound
KR100301756B1 (en) Manufacturing method of 0,0'- diacyl tartaric anhydride and manufacturing method of 0,0'- diacyl tartaric acid
GB2123821A (en) ???-Halogeno diethyl carbonates and their use in the preparation of antibiotics
GB2168050A (en) Intermediates useful in the preparation of antibiotics
KR870001806B1 (en) Process for preparing antibiotics
CS251086B2 (en) Method of g-penicilin's ethoxycarbonyloxyethylester production
IE56713B1 (en) Alpha-bromodiethylcarbonate and its use in the preparation of antibiotics
EP0611758B1 (en) Process for synthesizing 4-halo-5(hydroxymethyl) imidazole compounds and certain novel 4-halo-5(hydroxymethyl) imidazole compounds
EP0423991B1 (en) Process for the preparation of 2-trifluoromethyl-4-hydroxybenzoic acid
KR0171671B1 (en) Production of 2',3'-dichloro-3-ethoxymethoxybenzanilide
NZ216265A (en) Alpha-bromodiethylcarbonate and its manufacture
FI79832C (en) α-Bromideethyl carbonate and its use in the preparation of the ethoxic rbonyloxyethyl ester of penicillin G
US5597941A (en) Process for production of 5-amino-3-methylpyrazole
SI8610218A8 (en) Process for preparing ethoxycarbonyloxyethylesther of penicilyne g
NL9900008A (en) Bacampicillin prepn. from ampicillin via enamine
GB2177698A (en) Improvements in the preparation of antibiotics
JPH078860B2 (en) Pyrazole derivative and method for producing the same
US5266704A (en) Process for production of 2-substituted-4-hydromethylimidazole compounds
JPH0641437B2 (en) Method for producing malonic acid derivative
FR2532933A1 (en) Improvements made to the preparation of antibiotics
JP2000229934A (en) Production of 13c-containing urea

Legal Events

Date Code Title Description
MKLA Lapsed