CA1313153C - Hybrid seed production - Google Patents
Hybrid seed productionInfo
- Publication number
- CA1313153C CA1313153C CA000573069A CA573069A CA1313153C CA 1313153 C CA1313153 C CA 1313153C CA 000573069 A CA000573069 A CA 000573069A CA 573069 A CA573069 A CA 573069A CA 1313153 C CA1313153 C CA 1313153C
- Authority
- CA
- Canada
- Prior art keywords
- marker gene
- plants
- plant
- nuclear marker
- phytotoxic
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 238000004519 manufacturing process Methods 0.000 title abstract description 30
- 238000000034 method Methods 0.000 claims abstract description 92
- 239000000126 substance Substances 0.000 claims abstract description 68
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 65
- 231100000208 phytotoxic Toxicity 0.000 claims abstract description 47
- 230000000885 phytotoxic effect Effects 0.000 claims abstract description 47
- 229930027917 kanamycin Natural products 0.000 claims description 62
- 229960000318 kanamycin Drugs 0.000 claims description 62
- SBUJHOSQTJFQJX-NOAMYHISSA-N kanamycin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N SBUJHOSQTJFQJX-NOAMYHISSA-N 0.000 claims description 62
- 229930182823 kanamycin A Natural products 0.000 claims description 62
- 239000003550 marker Substances 0.000 claims description 37
- 230000009466 transformation Effects 0.000 claims description 26
- 239000004009 herbicide Substances 0.000 claims description 18
- 238000012546 transfer Methods 0.000 claims description 14
- 230000002363 herbicidal effect Effects 0.000 claims description 12
- 238000003306 harvesting Methods 0.000 claims description 11
- 230000003115 biocidal effect Effects 0.000 claims description 9
- 230000001404 mediated effect Effects 0.000 claims description 8
- 210000001938 protoplast Anatomy 0.000 claims description 7
- 239000003242 anti bacterial agent Substances 0.000 claims description 6
- 230000004720 fertilization Effects 0.000 claims description 6
- 230000004927 fusion Effects 0.000 claims description 6
- 230000001939 inductive effect Effects 0.000 claims description 6
- 238000002703 mutagenesis Methods 0.000 claims description 6
- 231100000350 mutagenesis Toxicity 0.000 claims description 6
- 210000001082 somatic cell Anatomy 0.000 claims description 6
- 238000004113 cell culture Methods 0.000 claims description 5
- 238000000844 transformation Methods 0.000 claims description 5
- 238000010998 test method Methods 0.000 claims description 4
- 239000000203 mixture Substances 0.000 claims description 3
- 229940088710 antibiotic agent Drugs 0.000 claims description 2
- 241000589158 Agrobacterium Species 0.000 claims 4
- 238000009396 hybridization Methods 0.000 abstract description 3
- 241000196324 Embryophyta Species 0.000 description 72
- 230000010153 self-pollination Effects 0.000 description 15
- 206010021929 Infertility male Diseases 0.000 description 10
- 208000007466 Male Infertility Diseases 0.000 description 10
- 238000011109 contamination Methods 0.000 description 9
- 230000010152 pollination Effects 0.000 description 8
- 238000013459 approach Methods 0.000 description 6
- 230000035558 fertility Effects 0.000 description 6
- 241000208125 Nicotiana Species 0.000 description 5
- 210000004027 cell Anatomy 0.000 description 5
- 239000002609 medium Substances 0.000 description 5
- 241001573881 Corolla Species 0.000 description 4
- 230000002068 genetic effect Effects 0.000 description 4
- 230000005849 recognition of pollen Effects 0.000 description 4
- 238000009331 sowing Methods 0.000 description 4
- 244000291564 Allium cepa Species 0.000 description 3
- 108010035563 Chloramphenicol O-acetyltransferase Proteins 0.000 description 3
- 238000003976 plant breeding Methods 0.000 description 3
- 230000035945 sensitivity Effects 0.000 description 3
- 238000005507 spraying Methods 0.000 description 3
- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 description 3
- 229920001817 Agar Polymers 0.000 description 2
- 241000589155 Agrobacterium tumefaciens Species 0.000 description 2
- 235000002732 Allium cepa var. cepa Nutrition 0.000 description 2
- 235000011331 Brassica Nutrition 0.000 description 2
- 241000219198 Brassica Species 0.000 description 2
- 108091026890 Coding region Proteins 0.000 description 2
- 108010066133 D-octopine dehydrogenase Proteins 0.000 description 2
- 240000001140 Mimosa pudica Species 0.000 description 2
- 235000016462 Mimosa pudica Nutrition 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 239000000306 component Substances 0.000 description 2
- 230000001086 cytosolic effect Effects 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 230000010159 dioecy Effects 0.000 description 2
- 238000002955 isolation Methods 0.000 description 2
- 239000013612 plasmid Substances 0.000 description 2
- 150000003839 salts Chemical class 0.000 description 2
- 238000012216 screening Methods 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 239000002689 soil Substances 0.000 description 2
- 230000002269 spontaneous effect Effects 0.000 description 2
- 239000007921 spray Substances 0.000 description 2
- 244000180534 Berberis hybrid Species 0.000 description 1
- 239000005496 Chlorsulfuron Substances 0.000 description 1
- 241001136239 Cymbidium hybrid cultivar Species 0.000 description 1
- IMXSCCDUAFEIOE-UHFFFAOYSA-N D-Octopin Natural products OC(=O)C(C)NC(C(O)=O)CCCN=C(N)N IMXSCCDUAFEIOE-UHFFFAOYSA-N 0.000 description 1
- IMXSCCDUAFEIOE-RITPCOANSA-N D-octopine Chemical compound [O-]C(=O)[C@@H](C)[NH2+][C@H](C([O-])=O)CCCNC(N)=[NH2+] IMXSCCDUAFEIOE-RITPCOANSA-N 0.000 description 1
- 108020004414 DNA Proteins 0.000 description 1
- 239000005980 Gibberellic acid Substances 0.000 description 1
- 239000005562 Glyphosate Substances 0.000 description 1
- 241000208818 Helianthus Species 0.000 description 1
- 235000003222 Helianthus annuus Nutrition 0.000 description 1
- 244000134716 Hodgsonia macrocarpa Species 0.000 description 1
- 240000005979 Hordeum vulgare Species 0.000 description 1
- 235000007340 Hordeum vulgare Nutrition 0.000 description 1
- 108010025815 Kanamycin Kinase Proteins 0.000 description 1
- 241000208133 Nicotiana plumbaginifolia Species 0.000 description 1
- 244000061176 Nicotiana tabacum Species 0.000 description 1
- 235000002637 Nicotiana tabacum Nutrition 0.000 description 1
- 208000012868 Overgrowth Diseases 0.000 description 1
- 241001632422 Radiola linoides Species 0.000 description 1
- 239000005708 Sodium hypochlorite Substances 0.000 description 1
- 241001291279 Solanum galapagense Species 0.000 description 1
- 240000006394 Sorghum bicolor Species 0.000 description 1
- 235000011684 Sorghum saccharatum Nutrition 0.000 description 1
- 244000300264 Spinacia oleracea Species 0.000 description 1
- 235000009337 Spinacia oleracea Nutrition 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 108010006873 Threonine Dehydratase Proteins 0.000 description 1
- 241000209140 Triticum Species 0.000 description 1
- 235000021307 Triticum Nutrition 0.000 description 1
- 240000000359 Triticum dicoccon Species 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- KRWTWSSMURUMDE-UHFFFAOYSA-N [1-(2-methoxynaphthalen-1-yl)naphthalen-2-yl]-diphenylphosphane Chemical compound COC1=CC=C2C=CC=CC2=C1C(C1=CC=CC=C1C=C1)=C1P(C=1C=CC=CC=1)C1=CC=CC=C1 KRWTWSSMURUMDE-UHFFFAOYSA-N 0.000 description 1
- GINJFDRNADDBIN-FXQIFTODSA-N bilanafos Chemical compound OC(=O)[C@H](C)NC(=O)[C@H](C)NC(=O)[C@@H](N)CCP(C)(O)=O GINJFDRNADDBIN-FXQIFTODSA-N 0.000 description 1
- 238000004166 bioassay Methods 0.000 description 1
- 231100000504 carcinogenesis Toxicity 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 229960004261 cefotaxime Drugs 0.000 description 1
- AZZMGZXNTDTSME-JUZDKLSSSA-M cefotaxime sodium Chemical compound [Na+].N([C@@H]1C(N2C(=C(COC(C)=O)CS[C@@H]21)C([O-])=O)=O)C(=O)\C(=N/OC)C1=CSC(N)=N1 AZZMGZXNTDTSME-JUZDKLSSSA-M 0.000 description 1
- VJYIFXVZLXQVHO-UHFFFAOYSA-N chlorsulfuron Chemical compound COC1=NC(C)=NC(NC(=O)NS(=O)(=O)C=2C(=CC=CC=2)Cl)=N1 VJYIFXVZLXQVHO-UHFFFAOYSA-N 0.000 description 1
- 235000005822 corn Nutrition 0.000 description 1
- 244000038559 crop plants Species 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000008030 elimination Effects 0.000 description 1
- 238000003379 elimination reaction Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000008124 floral development Effects 0.000 description 1
- 238000010353 genetic engineering Methods 0.000 description 1
- 230000035784 germination Effects 0.000 description 1
- IXORZMNAPKEEDV-UHFFFAOYSA-N gibberellic acid GA3 Natural products OC(=O)C1C2(C3)CC(=C)C3(O)CCC2C2(C=CC3O)C1C3(C)C(=O)O2 IXORZMNAPKEEDV-UHFFFAOYSA-N 0.000 description 1
- IXORZMNAPKEEDV-OBDJNFEBSA-N gibberellin A3 Chemical compound C([C@@]1(O)C(=C)C[C@@]2(C1)[C@H]1C(O)=O)C[C@H]2[C@]2(C=C[C@@H]3O)[C@H]1[C@]3(C)C(=O)O2 IXORZMNAPKEEDV-OBDJNFEBSA-N 0.000 description 1
- XDDAORKBJWWYJS-UHFFFAOYSA-N glyphosate Chemical compound OC(=O)CNCP(O)(O)=O XDDAORKBJWWYJS-UHFFFAOYSA-N 0.000 description 1
- 229940097068 glyphosate Drugs 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- ZTMKADLOSYKWCA-UHFFFAOYSA-N lenacil Chemical compound O=C1NC=2CCCC=2C(=O)N1C1CCCCC1 ZTMKADLOSYKWCA-UHFFFAOYSA-N 0.000 description 1
- 108010083942 mannopine synthase Proteins 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 230000035772 mutation Effects 0.000 description 1
- 239000000049 pigment Substances 0.000 description 1
- 230000019612 pigmentation Effects 0.000 description 1
- 239000003375 plant hormone Substances 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000012021 retail method of payment Methods 0.000 description 1
- 230000008771 sex reversal Effects 0.000 description 1
- SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 description 1
- GABUSZPTCJGKGB-UHFFFAOYSA-M sodium;4-(4-chloro-2-methylphenoxy)butanoate Chemical compound [Na+].CC1=CC(Cl)=CC=C1OCCCC([O-])=O GABUSZPTCJGKGB-UHFFFAOYSA-M 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 230000009261 transgenic effect Effects 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8287—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for fertility modification, e.g. apomixis
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Developmental Biology & Embryology (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Environmental Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Botany (AREA)
- Organic Chemistry (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Pretreatment Of Seeds And Plants (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Cultivation Of Plants (AREA)
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| NZ22126787A NZ221267A (en) | 1987-07-30 | 1987-07-30 | Hybrid seed production using a phytotoxic chemical to eliminate undesirable seeds |
| NZ221267 | 1987-07-30 | ||
| NZ221375 | 1987-08-07 | ||
| NZ221375A NZ221375A (en) | 1987-07-30 | 1987-08-07 | Hybrid seed production using a phytotoxic chemical to eliminate undesirable seeds |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA1313153C true CA1313153C (en) | 1993-01-26 |
Family
ID=26650756
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA000573069A Expired - Fee Related CA1313153C (en) | 1987-07-30 | 1988-07-26 | Hybrid seed production |
Country Status (11)
| Country | Link |
|---|---|
| JP (1) | JPH02402A (sv) |
| AU (1) | AU615460B2 (sv) |
| CA (1) | CA1313153C (sv) |
| DE (1) | DE3825492A1 (sv) |
| DK (1) | DK390288A (sv) |
| FR (1) | FR2618640A1 (sv) |
| GB (1) | GB2208346B (sv) |
| IT (1) | IT1229991B (sv) |
| NL (1) | NL8801888A (sv) |
| NZ (1) | NZ221375A (sv) |
| SE (1) | SE8802764L (sv) |
Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0825262A3 (en) * | 1989-12-29 | 1998-03-18 | Novartis AG | Tissue-specific toxin expression in plants |
| GB9014090D0 (en) * | 1990-06-25 | 1990-08-15 | Zaadunie Bv | Improvements in or relating to organic compounds |
| GB9313975D0 (en) * | 1993-07-06 | 1993-08-18 | Sandoz Ltd | Improvements in or relating to organic compounds |
| US5717129A (en) * | 1995-02-16 | 1998-02-10 | Pioneer Hi-Bred International, Inc. | Methods for maintaining sterility in plants |
| US6066779A (en) * | 1997-04-28 | 2000-05-23 | Yan's Heterosis & Herbicide, Inc. | Crop heterosis and herbicide |
| JP3261455B2 (ja) * | 1999-10-08 | 2002-03-04 | 独立行政法人 農業技術研究機構 | イネ科植物の花粉飛散防止方法 |
| FR2802768B1 (fr) * | 1999-12-23 | 2002-03-01 | Limagrain Sa | Procede de determination du pourcentage de semences porteuses d'au moins un gene d'interet |
| US6646186B1 (en) * | 2000-07-26 | 2003-11-11 | Stine Seed Farm Inc. | Hybrid soybeans and methods of production |
| KR100849166B1 (ko) * | 2005-02-07 | 2008-07-30 | 인제대학교 산학협력단 | 유디피-글루쿠로노실트랜스퍼라제 1에이4 유전자의단일염기 다형성 및 그 용도 |
| KR100816018B1 (ko) * | 2007-01-19 | 2008-03-21 | 한국과학기술원 | Ｆｏｃｕｓｓ 알고리듬을 이용한 고해상도 영상 복원 방법 |
| EP3802886A1 (en) * | 2018-05-25 | 2021-04-14 | Philip Morris Products S.A. | Method for classifying plant material |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| HU165923B (sv) * | 1972-03-13 | 1974-12-28 | ||
| WO1983001176A1 (en) * | 1981-10-01 | 1983-04-14 | Int Plant Research Inst | Process for the genetic modification of cereals with transformation vectors |
| EP0320500B1 (en) * | 1983-01-13 | 2004-11-17 | Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. | Non-oncogenic ti plasmid vector system and recombinant DNA molecules for the introduction of expressible genes into plant cell genomes |
| US4517763A (en) * | 1983-05-11 | 1985-05-21 | University Of Guelph | Hybridization process utilizing a combination of cytoplasmic male sterility and herbicide tolerance |
| US4570380A (en) * | 1984-11-28 | 1986-02-18 | Ciba-Geigy Corporation | Route to hybrid cotton production |
| US5180873A (en) * | 1985-04-16 | 1993-01-19 | Dna Plant Technology Corporation | Transformation of plants to introduce closely linked markers |
| US4658084A (en) * | 1985-11-14 | 1987-04-14 | University Of Guelph | Hybridization using cytoplasmic male sterility and herbicide tolerance from nuclear genes |
| US4658085A (en) * | 1985-11-14 | 1987-04-14 | University Of Guelph | Hybridization using cytoplasmic male sterility, cytoplasmic herbicide tolerance, and herbicide tolerance from nuclear genes |
-
1987
- 1987-08-07 NZ NZ221375A patent/NZ221375A/xx unknown
-
1988
- 1988-07-12 DK DK390288A patent/DK390288A/da not_active Application Discontinuation
- 1988-07-19 GB GB8817191A patent/GB2208346B/en not_active Expired - Fee Related
- 1988-07-26 CA CA000573069A patent/CA1313153C/en not_active Expired - Fee Related
- 1988-07-27 DE DE3825492A patent/DE3825492A1/de not_active Withdrawn
- 1988-07-27 NL NL8801888A patent/NL8801888A/nl not_active Application Discontinuation
- 1988-07-27 IT IT8821521A patent/IT1229991B/it active
- 1988-07-27 JP JP63187959A patent/JPH02402A/ja active Pending
- 1988-07-28 FR FR8810189A patent/FR2618640A1/fr active Pending
- 1988-07-29 AU AU20210/88A patent/AU615460B2/en not_active Ceased
- 1988-07-29 SE SE8802764A patent/SE8802764L/sv not_active Application Discontinuation
Also Published As
| Publication number | Publication date |
|---|---|
| DK390288D0 (da) | 1988-07-12 |
| GB8817191D0 (en) | 1988-08-24 |
| FR2618640A1 (fr) | 1989-02-03 |
| SE8802764D0 (sv) | 1988-07-29 |
| DK390288A (da) | 1989-01-31 |
| IT1229991B (it) | 1991-09-20 |
| NL8801888A (nl) | 1989-02-16 |
| AU615460B2 (en) | 1991-10-03 |
| JPH02402A (ja) | 1990-01-05 |
| DE3825492A1 (de) | 1989-02-09 |
| SE8802764A0 (sv) | 1989-01-31 |
| AU2021088A (en) | 1989-02-02 |
| IT8821521A0 (it) | 1988-07-27 |
| SE8802764L (sv) | 1989-01-31 |
| GB2208346B (en) | 1991-07-03 |
| GB2208346A (en) | 1989-03-30 |
| NZ221375A (en) | 1990-09-26 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| MKLA | Lapsed |