CA1312565C - Molecular probes for dna specific for the male genome of ruminants, especially of the subfamily of the bovines and particularly of the genus bos, the process for their preparationand the use of these probes for determining the sex of ruminant embryos and fetuses and for checking the presence of the y chromosome - Google Patents
Molecular probes for dna specific for the male genome of ruminants, especially of the subfamily of the bovines and particularly of the genus bos, the process for their preparationand the use of these probes for determining the sex of ruminant embryos and fetuses and for checking the presence of the y chromosomeInfo
- Publication number
- CA1312565C CA1312565C CA 530626 CA530626A CA1312565C CA 1312565 C CA1312565 C CA 1312565C CA 530626 CA530626 CA 530626 CA 530626 A CA530626 A CA 530626A CA 1312565 C CA1312565 C CA 1312565C
- Authority
- CA
- Canada
- Prior art keywords
- spermatozoa
- dna
- chromosome
- probe
- hybridization
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
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- AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 1
- 229960000723 ampicillin Drugs 0.000 description 1
- 150000008064 anhydrides Chemical class 0.000 description 1
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- HAAZLUGHYHWQIW-KVQBGUIXSA-N dGTP Chemical compound C1=NC=2C(=O)NC(N)=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 HAAZLUGHYHWQIW-KVQBGUIXSA-N 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
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- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- VHJLVAABSRFDPM-QWWZWVQMSA-N dithiothreitol Chemical compound SC[C@@H](O)[C@H](O)CS VHJLVAABSRFDPM-QWWZWVQMSA-N 0.000 description 1
- MOTZDAYCYVMXPC-UHFFFAOYSA-N dodecyl hydrogen sulfate Chemical compound CCCCCCCCCCCCOS(O)(=O)=O MOTZDAYCYVMXPC-UHFFFAOYSA-N 0.000 description 1
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- 239000000975 dye Substances 0.000 description 1
- 210000005069 ears Anatomy 0.000 description 1
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- 150000002148 esters Chemical class 0.000 description 1
- ZMMJGEGLRURXTF-UHFFFAOYSA-N ethidium bromide Chemical compound [Br-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CC)=C1C1=CC=CC=C1 ZMMJGEGLRURXTF-UHFFFAOYSA-N 0.000 description 1
- 229960005542 ethidium bromide Drugs 0.000 description 1
- 230000001747 exhibiting effect Effects 0.000 description 1
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- 150000004665 fatty acids Chemical class 0.000 description 1
- 230000035558 fertility Effects 0.000 description 1
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- 229910052588 hydroxylapatite Inorganic materials 0.000 description 1
- JCYWCSGERIELPG-UHFFFAOYSA-N imes Chemical class CC1=CC(C)=CC(C)=C1N1C=CN(C=2C(=CC(C)=CC=2C)C)[C]1 JCYWCSGERIELPG-UHFFFAOYSA-N 0.000 description 1
- 230000036512 infertility Effects 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
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- 238000004519 manufacturing process Methods 0.000 description 1
- LWJROJCJINYWOX-UHFFFAOYSA-L mercury dichloride Chemical compound Cl[Hg]Cl LWJROJCJINYWOX-UHFFFAOYSA-L 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- 238000005065 mining Methods 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- RJMUSRYZPJIFPJ-UHFFFAOYSA-N niclosamide Chemical compound OC1=CC=C(Cl)C=C1C(=O)NC1=CC=C([N+]([O-])=O)C=C1Cl RJMUSRYZPJIFPJ-UHFFFAOYSA-N 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 210000004940 nucleus Anatomy 0.000 description 1
- 239000002674 ointment Substances 0.000 description 1
- 150000002894 organic compounds Chemical class 0.000 description 1
- 230000007170 pathology Effects 0.000 description 1
- XYJRXVWERLGGKC-UHFFFAOYSA-D pentacalcium;hydroxide;triphosphate Chemical compound [OH-].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O XYJRXVWERLGGKC-UHFFFAOYSA-D 0.000 description 1
- DDBREPKUVSBGFI-UHFFFAOYSA-N phenobarbital Chemical compound C=1C=CC=CC=1C1(CC)C(=O)NC(=O)NC1=O DDBREPKUVSBGFI-UHFFFAOYSA-N 0.000 description 1
- HCTVWSOKIJULET-LQDWTQKMSA-M phenoxymethylpenicillin potassium Chemical compound [K+].N([C@H]1[C@H]2SC([C@@H](N2C1=O)C([O-])=O)(C)C)C(=O)COC1=CC=CC=C1 HCTVWSOKIJULET-LQDWTQKMSA-M 0.000 description 1
- 150000008300 phosphoramidites Chemical class 0.000 description 1
- 102000020233 phosphotransferase Human genes 0.000 description 1
- 238000000053 physical method Methods 0.000 description 1
- 229920002401 polyacrylamide Polymers 0.000 description 1
- 229920002647 polyamide Polymers 0.000 description 1
- 229920000570 polyether Polymers 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- -1 polyoxyethylene Polymers 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 239000001103 potassium chloride Substances 0.000 description 1
- 235000011164 potassium chloride Nutrition 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- INCIMLINXXICKS-UHFFFAOYSA-M pyronin Y Chemical compound [Cl-].C1=CC(=[N+](C)C)C=C2OC3=CC(N(C)C)=CC=C3C=C21 INCIMLINXXICKS-UHFFFAOYSA-M 0.000 description 1
- 238000000163 radioactive labelling Methods 0.000 description 1
- 239000011535 reaction buffer Substances 0.000 description 1
- 235000002020 sage Nutrition 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 229920006395 saturated elastomer Polymers 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 210000003765 sex chromosome Anatomy 0.000 description 1
- 230000020509 sex determination Effects 0.000 description 1
- 230000001568 sexual effect Effects 0.000 description 1
- 150000003378 silver Chemical class 0.000 description 1
- 229910052979 sodium sulfide Inorganic materials 0.000 description 1
- GRVFOGOEDUUMBP-UHFFFAOYSA-N sodium sulfide (anhydrous) Chemical compound [Na+].[Na+].[S-2] GRVFOGOEDUUMBP-UHFFFAOYSA-N 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 229940063673 spermidine Drugs 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 150000003467 sulfuric acid derivatives Chemical class 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 238000013519 translation Methods 0.000 description 1
- 239000001226 triphosphate Substances 0.000 description 1
- 235000011178 triphosphate Nutrition 0.000 description 1
- UNXRWKVEANCORM-UHFFFAOYSA-N triphosphoric acid Chemical compound OP(O)(=O)OP(O)(=O)OP(O)(O)=O UNXRWKVEANCORM-UHFFFAOYSA-N 0.000 description 1
- 238000012800 visualization Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6879—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for sex determination
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1072—Differential gene expression library synthesis, e.g. subtracted libraries, differential screening
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Analytical Chemistry (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Bioinformatics & Computational Biology (AREA)
- Immunology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FR8602811 | 1986-02-28 | ||
| FR8602811A FR2595100B1 (fr) | 1986-02-28 | 1986-02-28 | Sondes moleculaires d'adn specifique du genome male de mammiferes, notamment du genre bos, presentant des sequences d'adn specifique du sexe male, procede de preparation de telles sondes et procede de determination du sexe d'embryons et de foetus des mammiferes susdits, a l'aide de ces sondes |
| FR8612616 | 1986-09-09 | ||
| FR8612616A FR2603701B2 (fr) | 1986-02-28 | 1986-09-09 | Applications des sondes moleculaires d'adn specifique du genome male de mammiferes, notamment du genre bos, au controle de la presence du chromosome y dans une population de spermatozoides et a la separation des spermatozoides en deux populations de spermatozoides porteurs respectivement du chromosome y et du chromosome x pour leur utilisation pour l'insemination artificielle |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA1312565C true CA1312565C (en) | 1993-01-12 |
Family
ID=26225052
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA 530626 Expired - Fee Related CA1312565C (en) | 1986-02-28 | 1987-02-25 | Molecular probes for dna specific for the male genome of ruminants, especially of the subfamily of the bovines and particularly of the genus bos, the process for their preparationand the use of these probes for determining the sex of ruminant embryos and fetuses and for checking the presence of the y chromosome |
Country Status (8)
| Country | Link |
|---|---|
| EP (1) | EP0235046B1 (en)) |
| AU (1) | AU596642B2 (en)) |
| CA (1) | CA1312565C (en)) |
| DE (1) | DE3786181T2 (en)) |
| ES (1) | ES2042591T3 (en)) |
| FR (1) | FR2603701B2 (en)) |
| IN (1) | IN167981B (en)) |
| NZ (1) | NZ219431A (en)) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20120094352A1 (en) * | 2008-07-15 | 2012-04-19 | Masterrind Gmbh | Method for cell identification and cell sorting |
Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU614494B2 (en) * | 1986-08-12 | 1991-09-05 | Australian National University, The | Sex determination in ruminants using y-chromosome specific polynucleotides |
| GB8716367D0 (en) * | 1987-07-10 | 1987-08-19 | Reeders S T | Genetic identification |
| FR2619819B1 (fr) * | 1987-08-27 | 1990-08-10 | Agronomique Inst Nat Rech | Sondes moleculaires d'adn specifique du genome male de ruminants, notamment de la sous-famille des bovines et en particulier du genre bos, sequences flanquantes pour l'amplification de ces sondes et applications desdites sondes |
| US5328827A (en) * | 1987-08-27 | 1994-07-12 | Institut National De La Recherche Agronomique - Inra | Specific DNA molecular probes for Bos-type male genome |
| WO1989002440A2 (en) * | 1987-09-21 | 1989-03-23 | Whitehead Institute For Biomedical Research | Y-specific dna hybridization probes and uses therefor |
| WO1989002934A1 (en) * | 1987-10-02 | 1989-04-06 | Microprobe Corporation | Human papillomavirus type diagnosis with nucleotide probes |
| WO1989007154A1 (en) * | 1988-01-29 | 1989-08-10 | Advanced Riverina Holdings Limited | Determination of genetic sex in ruminants using y-chromosome-specific polynucleotides |
| FR2635116B1 (fr) * | 1988-08-08 | 1990-11-02 | Georges Michel | Procede pour la determination du sexe d'embryons de ruminants, kit ou necessaire pour la mise en oeuvre du procede |
| FR2648151B1 (fr) * | 1989-06-08 | 1991-12-20 | Michel Georges | Minisatellite bovin specifique au chromosome y des bovines |
| US5055393A (en) * | 1989-06-13 | 1991-10-08 | Salk Institute Biotechnology/Industrial Associates, Inc. | Prenatal sex determination of bovine cells using male-specific oligonucleotides |
| DE19959751A1 (de) * | 1999-12-11 | 2001-06-13 | Hans Rudolf Fries | DNA-Marker für die Erstellung von digitalen DNA-Signaturen |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4448767A (en) * | 1977-10-11 | 1984-05-15 | Sumar Corporation | Preparation of monospecific male-specific antibody and the use thereof for increasing the percentage of mammalian offspring of either sex |
| AU8007182A (en) * | 1980-12-18 | 1982-07-01 | Yale University | Method for cloning genes |
| WO1984001265A1 (en) * | 1982-10-05 | 1984-04-12 | Genetic Engineering Inc | Method of treating collected mammal semen and separating sperm into x and y components |
| FR2566911A1 (fr) * | 1984-06-29 | 1986-01-03 | Pasteur Institut | Sonde et procede pour le diagnostic du sexe d'un embryon humain |
| US4769319A (en) * | 1985-05-31 | 1988-09-06 | Salk Institute Biotechnology Industrial Associates, Inc. | Nucleic acid probes for prenatal sexing |
| AU4727185A (en) * | 1985-08-20 | 1987-03-10 | Apothekernes Laboratorium A.S. | Photoimmune detection of dna and rna |
-
1986
- 1986-09-09 FR FR8612616A patent/FR2603701B2/fr not_active Expired - Lifetime
-
1987
- 1987-02-24 AU AU69203/87A patent/AU596642B2/en not_active Ceased
- 1987-02-25 CA CA 530626 patent/CA1312565C/en not_active Expired - Fee Related
- 1987-02-27 NZ NZ21943187A patent/NZ219431A/en unknown
- 1987-02-27 DE DE19873786181 patent/DE3786181T2/de not_active Expired - Fee Related
- 1987-02-27 ES ES87400434T patent/ES2042591T3/es not_active Expired - Lifetime
- 1987-02-27 EP EP87400434A patent/EP0235046B1/fr not_active Expired - Lifetime
- 1987-02-27 IN IN150/CAL/87A patent/IN167981B/en unknown
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20120094352A1 (en) * | 2008-07-15 | 2012-04-19 | Masterrind Gmbh | Method for cell identification and cell sorting |
| US8649983B2 (en) * | 2008-07-15 | 2014-02-11 | Masterrind, GmbH | Method for cell identification and cell sorting |
Also Published As
| Publication number | Publication date |
|---|---|
| ES2042591T3 (es) | 1993-12-16 |
| EP0235046A1 (fr) | 1987-09-02 |
| NZ219431A (en) | 1989-04-26 |
| FR2603701B2 (fr) | 1990-09-21 |
| EP0235046B1 (fr) | 1993-06-16 |
| DE3786181D1 (de) | 1993-07-22 |
| AU596642B2 (en) | 1990-05-10 |
| DE3786181T2 (de) | 1993-09-23 |
| AU6920387A (en) | 1987-09-03 |
| FR2603701A2 (fr) | 1988-03-11 |
| IN167981B (en)) | 1991-01-19 |
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Legal Events
| Date | Code | Title | Description |
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| MKLA | Lapsed |