CA1215903A - Assay method for component relating to lipids, composition for assay and process for production of enzyme used therefor - Google Patents
Assay method for component relating to lipids, composition for assay and process for production of enzyme used thereforInfo
- Publication number
- CA1215903A CA1215903A CA000405920A CA405920A CA1215903A CA 1215903 A CA1215903 A CA 1215903A CA 000405920 A CA000405920 A CA 000405920A CA 405920 A CA405920 A CA 405920A CA 1215903 A CA1215903 A CA 1215903A
- Authority
- CA
- Canada
- Prior art keywords
- activity
- assay
- composition
- coa
- fatty acid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 238000000034 method Methods 0.000 title claims abstract description 24
- 230000008569 process Effects 0.000 title claims abstract description 13
- 102000004190 Enzymes Human genes 0.000 title claims description 152
- 108090000790 Enzymes Proteins 0.000 title claims description 152
- 238000003556 assay Methods 0.000 title claims description 151
- 239000000203 mixture Substances 0.000 title claims description 103
- 238000004519 manufacturing process Methods 0.000 title description 8
- 150000002632 lipids Chemical class 0.000 title 1
- 235000014113 dietary fatty acids Nutrition 0.000 claims abstract description 113
- 229930195729 fatty acid Natural products 0.000 claims abstract description 113
- 239000000194 fatty acid Substances 0.000 claims abstract description 113
- 150000004665 fatty acids Chemical class 0.000 claims abstract description 66
- 125000002252 acyl group Chemical group 0.000 claims abstract description 8
- 125000004432 carbon atom Chemical group C* 0.000 claims abstract description 7
- 230000000694 effects Effects 0.000 claims description 188
- 238000006243 chemical reaction Methods 0.000 claims description 59
- -1 fatty acid ester Chemical class 0.000 claims description 51
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 41
- 230000002829 reductive effect Effects 0.000 claims description 35
- IIZPXYDJLKNOIY-JXPKJXOSSA-N 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCC\C=C/C\C=C/C\C=C/C\C=C/CCCCC IIZPXYDJLKNOIY-JXPKJXOSSA-N 0.000 claims description 30
- 239000000787 lecithin Substances 0.000 claims description 30
- 235000010445 lecithin Nutrition 0.000 claims description 30
- 229940067606 lecithin Drugs 0.000 claims description 30
- RGJOEKWQDUBAIZ-IBOSZNHHSA-N CoASH Chemical compound O[C@@H]1[C@H](OP(O)(O)=O)[C@@H](COP(O)(=O)OP(O)(=O)OCC(C)(C)[C@@H](O)C(=O)NCCC(=O)NCCS)O[C@H]1N1C2=NC=NC(N)=C2N=C1 RGJOEKWQDUBAIZ-IBOSZNHHSA-N 0.000 claims description 27
- RGJOEKWQDUBAIZ-UHFFFAOYSA-N coenzime A Natural products OC1C(OP(O)(O)=O)C(COP(O)(=O)OP(O)(=O)OCC(C)(C)C(O)C(=O)NCCC(=O)NCCS)OC1N1C2=NC=NC(N)=C2N=C1 RGJOEKWQDUBAIZ-UHFFFAOYSA-N 0.000 claims description 27
- 102000052553 3-Hydroxyacyl CoA Dehydrogenase Human genes 0.000 claims description 25
- 108700020831 3-Hydroxyacyl-CoA Dehydrogenase Proteins 0.000 claims description 25
- 102000005870 Coenzyme A Ligases Human genes 0.000 claims description 21
- 239000004367 Lipase Substances 0.000 claims description 21
- 108010011449 Long-chain-fatty-acid-CoA ligase Proteins 0.000 claims description 21
- 102000004672 Acetyl-CoA C-acyltransferase Human genes 0.000 claims description 20
- 108010003902 Acetyl-CoA C-acyltransferase Proteins 0.000 claims description 20
- 108010023922 Enoyl-CoA hydratase Proteins 0.000 claims description 20
- 102000011426 Enoyl-CoA hydratase Human genes 0.000 claims description 20
- 102000004882 Lipase Human genes 0.000 claims description 20
- 108090001060 Lipase Proteins 0.000 claims description 20
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 claims description 20
- 235000019421 lipase Nutrition 0.000 claims description 20
- 235000019626 lipase activity Nutrition 0.000 claims description 20
- 108020002496 Lysophospholipase Proteins 0.000 claims description 19
- 102000004539 Acyl-CoA Oxidase Human genes 0.000 claims description 18
- 108020001558 Acyl-CoA oxidase Proteins 0.000 claims description 18
- 102100031415 Hepatic triacylglycerol lipase Human genes 0.000 claims description 16
- 102100035687 Bile salt-activated lipase Human genes 0.000 claims description 14
- 102100037883 Phospholipase B1, membrane-associated Human genes 0.000 claims description 14
- 108010055297 Sterol Esterase Proteins 0.000 claims description 14
- 244000005700 microbiome Species 0.000 claims description 11
- 102000001107 Phosphatidate Phosphatase Human genes 0.000 claims description 10
- 108010069394 Phosphatidate Phosphatase Proteins 0.000 claims description 10
- 241000589538 Pseudomonas fragi Species 0.000 claims description 10
- 102000014384 Type C Phospholipases Human genes 0.000 claims description 10
- 108010079194 Type C Phospholipases Proteins 0.000 claims description 10
- 235000012000 cholesterol Nutrition 0.000 claims description 10
- UFTFJSFQGQCHQW-UHFFFAOYSA-N triformin Chemical compound O=COCC(OC=O)COC=O UFTFJSFQGQCHQW-UHFFFAOYSA-N 0.000 claims description 9
- 101710088194 Dehydrogenase Proteins 0.000 claims description 8
- 102000011420 Phospholipase D Human genes 0.000 claims description 8
- 108090000553 Phospholipase D Proteins 0.000 claims description 8
- 238000010521 absorption reaction Methods 0.000 claims description 8
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 8
- 238000007254 oxidation reaction Methods 0.000 claims description 8
- 239000001301 oxygen Substances 0.000 claims description 8
- 229910052760 oxygen Inorganic materials 0.000 claims description 8
- 108010058864 Phospholipases A2 Proteins 0.000 claims description 7
- ASWBNKHCZGQVJV-UHFFFAOYSA-N (3-hexadecanoyloxy-2-hydroxypropyl) 2-(trimethylazaniumyl)ethyl phosphate Chemical group CCCCCCCCCCCCCCCC(=O)OCC(O)COP([O-])(=O)OCC[N+](C)(C)C ASWBNKHCZGQVJV-UHFFFAOYSA-N 0.000 claims description 6
- 108090000371 Esterases Proteins 0.000 claims description 6
- 229960001231 choline Drugs 0.000 claims description 6
- 150000001840 cholesterol esters Chemical class 0.000 claims description 5
- OEYIOHPDSNJKLS-UHFFFAOYSA-N choline Chemical compound C[N+](C)(C)CCO OEYIOHPDSNJKLS-UHFFFAOYSA-N 0.000 claims description 5
- LDVVTQMJQSCDMK-UHFFFAOYSA-N 1,3-dihydroxypropan-2-yl formate Chemical group OCC(CO)OC=O LDVVTQMJQSCDMK-UHFFFAOYSA-N 0.000 claims description 4
- 108010088751 Albumins Proteins 0.000 claims description 4
- 102000009027 Albumins Human genes 0.000 claims description 4
- 108010013563 Lipoprotein Lipase Proteins 0.000 claims description 4
- 159000000003 magnesium salts Chemical class 0.000 claims description 3
- 125000003831 tetrazolyl group Chemical group 0.000 claims description 3
- 230000001131 transforming effect Effects 0.000 claims description 3
- JLVVSXFLKOJNIY-UHFFFAOYSA-N Magnesium ion Chemical compound [Mg+2] JLVVSXFLKOJNIY-UHFFFAOYSA-N 0.000 claims description 2
- 229930182558 Sterol Natural products 0.000 claims description 2
- 229910001425 magnesium ion Inorganic materials 0.000 claims description 2
- 235000003702 sterols Nutrition 0.000 claims description 2
- 125000004435 hydrogen atom Chemical group [H]* 0.000 claims 8
- 102000001494 Sterol O-Acyltransferase Human genes 0.000 claims 3
- 108010054082 Sterol O-acyltransferase Proteins 0.000 claims 3
- PORPENFLTBBHSG-MGBGTMOVSA-N 1,2-dihexadecanoyl-sn-glycerol-3-phosphate Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP(O)(O)=O)OC(=O)CCCCCCCCCCCCCCC PORPENFLTBBHSG-MGBGTMOVSA-N 0.000 claims 1
- 238000004737 colorimetric analysis Methods 0.000 claims 1
- 125000005539 phosphatidic acid group Chemical group 0.000 claims 1
- 150000003248 quinolines Chemical group 0.000 claims 1
- 235000021588 free fatty acids Nutrition 0.000 abstract description 14
- 150000002148 esters Chemical class 0.000 abstract description 5
- 229940088598 enzyme Drugs 0.000 description 101
- 239000000243 solution Substances 0.000 description 29
- 239000000872 buffer Substances 0.000 description 26
- 230000015572 biosynthetic process Effects 0.000 description 19
- ZKHQWZAMYRWXGA-UHFFFAOYSA-N Adenosine triphosphate Natural products C1=NC=2C(N)=NC=NC=2N1C1OC(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)C(O)C1O ZKHQWZAMYRWXGA-UHFFFAOYSA-N 0.000 description 18
- 210000002966 serum Anatomy 0.000 description 18
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 16
- 239000002253 acid Substances 0.000 description 15
- 239000012153 distilled water Substances 0.000 description 15
- 229940040461 lipase Drugs 0.000 description 15
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 14
- WRIDQFICGBMAFQ-UHFFFAOYSA-N (E)-8-Octadecenoic acid Natural products CCCCCCCCCC=CCCCCCCC(O)=O WRIDQFICGBMAFQ-UHFFFAOYSA-N 0.000 description 13
- LQJBNNIYVWPHFW-UHFFFAOYSA-N 20:1omega9c fatty acid Natural products CCCCCCCCCCC=CCCCCCCCC(O)=O LQJBNNIYVWPHFW-UHFFFAOYSA-N 0.000 description 13
- QSBYPNXLFMSGKH-UHFFFAOYSA-N 9-Heptadecensaeure Natural products CCCCCCCC=CCCCCCCCC(O)=O QSBYPNXLFMSGKH-UHFFFAOYSA-N 0.000 description 13
- 239000005642 Oleic acid Substances 0.000 description 13
- ZQPPMHVWECSIRJ-UHFFFAOYSA-N Oleic acid Natural products CCCCCCCCC=CCCCCCCCC(O)=O ZQPPMHVWECSIRJ-UHFFFAOYSA-N 0.000 description 13
- QXJSBBXBKPUZAA-UHFFFAOYSA-N isooleic acid Natural products CCCCCCCC=CCCCCCCCCC(O)=O QXJSBBXBKPUZAA-UHFFFAOYSA-N 0.000 description 13
- ZQPPMHVWECSIRJ-KTKRTIGZSA-N oleic acid Chemical compound CCCCCCCC\C=C/CCCCCCCC(O)=O ZQPPMHVWECSIRJ-KTKRTIGZSA-N 0.000 description 13
- 235000021313 oleic acid Nutrition 0.000 description 13
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 11
- 241000589516 Pseudomonas Species 0.000 description 11
- 239000002609 medium Substances 0.000 description 11
- 102000004316 Oxidoreductases Human genes 0.000 description 10
- 108090000854 Oxidoreductases Proteins 0.000 description 10
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 9
- 102000016938 Catalase Human genes 0.000 description 9
- 108010053835 Catalase Proteins 0.000 description 9
- 230000009471 action Effects 0.000 description 9
- 230000007062 hydrolysis Effects 0.000 description 9
- 238000006460 hydrolysis reaction Methods 0.000 description 9
- 239000008363 phosphate buffer Substances 0.000 description 9
- UDMBCSSLTHHNCD-UHFFFAOYSA-N Coenzym Q(11) Natural products C1=NC=2C(N)=NC=NC=2N1C1OC(COP(O)(O)=O)C(O)C1O UDMBCSSLTHHNCD-UHFFFAOYSA-N 0.000 description 8
- 229920004890 Triton X-100 Polymers 0.000 description 8
- 239000013504 Triton X-100 Substances 0.000 description 8
- UDMBCSSLTHHNCD-KQYNXXCUSA-N adenosine 5'-monophosphate Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP(O)(O)=O)[C@@H](O)[C@H]1O UDMBCSSLTHHNCD-KQYNXXCUSA-N 0.000 description 8
- LNQVTSROQXJCDD-UHFFFAOYSA-N adenosine monophosphate Natural products C1=NC=2C(N)=NC=NC=2N1C1OC(CO)C(OP(O)(O)=O)C1O LNQVTSROQXJCDD-UHFFFAOYSA-N 0.000 description 8
- IPCSVZSSVZVIGE-UHFFFAOYSA-N hexadecanoic acid Chemical compound CCCCCCCCCCCCCCCC(O)=O IPCSVZSSVZVIGE-UHFFFAOYSA-N 0.000 description 8
- 239000011541 reaction mixture Substances 0.000 description 8
- 239000000758 substrate Substances 0.000 description 8
- 239000003153 chemical reaction reagent Substances 0.000 description 7
- 125000005456 glyceride group Chemical group 0.000 description 7
- 229960002163 hydrogen peroxide Drugs 0.000 description 7
- ROWKJAVDOGWPAT-UHFFFAOYSA-N Acetoin Chemical compound CC(O)C(C)=O ROWKJAVDOGWPAT-UHFFFAOYSA-N 0.000 description 6
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- 238000005119 centrifugation Methods 0.000 description 5
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- 210000004185 liver Anatomy 0.000 description 5
- NQMPLXPCRJOSHL-BBECNAHFSA-N 3-oxopalmitoyl-CoA Chemical compound O[C@@H]1[C@H](OP(O)(O)=O)[C@@H](COP(O)(=O)OP(O)(=O)OCC(C)(C)[C@@H](O)C(=O)NCCC(=O)NCCSC(=O)CC(=O)CCCCCCCCCCCCC)O[C@H]1N1C2=NC=NC(N)=C2N=C1 NQMPLXPCRJOSHL-BBECNAHFSA-N 0.000 description 4
- RLFWWDJHLFCNIJ-UHFFFAOYSA-N 4-aminoantipyrine Chemical compound CN1C(C)=C(N)C(=O)N1C1=CC=CC=C1 RLFWWDJHLFCNIJ-UHFFFAOYSA-N 0.000 description 4
- 108010001058 Acyl-CoA Dehydrogenase Proteins 0.000 description 4
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y103/00—Oxidoreductases acting on the CH-CH group of donors (1.3)
- C12Y103/99—Oxidoreductases acting on the CH-CH group of donors (1.3) with other acceptors (1.3.99)
- C12Y103/99003—Acyl-CoA dehydrogenase (1.3.99.3)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/001—Oxidoreductases (1.) acting on the CH-CH group of donors (1.3)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1025—Acyltransferases (2.3)
- C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/88—Lyases (4.)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/25—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving enzymes not classifiable in groups C12Q1/26 - C12Q1/66
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/8215—Microorganisms
- Y10S435/822—Microorganisms using bacteria or actinomycetales
- Y10S435/874—Pseudomonas
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biophysics (AREA)
- Immunology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP9931481A JPS58888A (ja) | 1981-06-25 | 1981-06-25 | 脂肪酸の分解に関与する酵素の製造法 |
| JP56-99314 | 1981-06-25 | ||
| JP14831381A JPS5851899A (ja) | 1981-09-18 | 1981-09-18 | 脂質関連成分の測定法および測定用組成物 |
| JP56-148313 | 1981-09-18 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA1215903A true CA1215903A (en) | 1986-12-30 |
Family
ID=26440452
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA000405920A Expired CA1215903A (en) | 1981-06-25 | 1982-06-24 | Assay method for component relating to lipids, composition for assay and process for production of enzyme used therefor |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US4491631A (en, 2012) |
| CA (1) | CA1215903A (en, 2012) |
| CH (1) | CH664631A5 (en, 2012) |
| DE (2) | DE3249973C2 (en, 2012) |
| FR (1) | FR2508487B1 (en, 2012) |
| GB (1) | GB2105843B (en, 2012) |
| IT (1) | IT1157281B (en, 2012) |
Families Citing this family (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5991898A (ja) * | 1982-11-19 | 1984-05-26 | Toyo Jozo Co Ltd | リパ−ゼ活性測定用組成物 |
| US5049488A (en) * | 1985-11-08 | 1991-09-17 | Genentech, Inc. | Method and nucleic acid for the preparation of lecithin:cholesterol acyltransferase |
| US5162201A (en) * | 1987-04-01 | 1992-11-10 | Toyo Jozo Co., Ltd. | Analytical method making use of monoglyceride lipase |
| DE3817747A1 (de) * | 1988-05-25 | 1989-11-30 | Johannes Dr Med Aufenanger | Verfahren zum bestimmen der relativen mengen aller cholesterinhaltigen lipoproteine in koerperfluessigkeiten |
| DE3926024C2 (de) * | 1989-08-07 | 1994-10-27 | Minnesota Mining & Mfg | Vorrichtung zum Entnehmen von fließfähigem Füllgut durch eine flexible Wand eines Beutels |
| EP0457901B1 (en) * | 1989-12-12 | 1995-11-08 | Lidak Pharmaceuticals | Free fatty acid determination |
| US7262017B2 (en) | 2001-09-14 | 2007-08-28 | Torrey Pines Institute For Molecular Studies | Diagnostic markers for ischemia |
| AU2005227312B2 (en) * | 2004-03-22 | 2011-04-07 | Ffa Sciences, Llp | Development and use of fluorescent probes of unbound analytes |
| CA2583546A1 (en) * | 2004-10-15 | 2006-04-27 | General Atomics | Enzyme cycling based assays for alpha-methylacyl-coa racemase |
| US7374897B2 (en) * | 2004-10-15 | 2008-05-20 | General Atomics | Enzyme cycling based assays for alpha-methylacyl-CoA racemase |
| US20060257938A1 (en) * | 2005-05-10 | 2006-11-16 | Kleinfeld Alan M | Method of screening for drugs that block ligand binding to a lipid binding protein |
| US9164109B2 (en) | 2006-10-27 | 2015-10-20 | Alan Kleinfeld | Use of probes for unbound metabolites |
| CN104245953B (zh) * | 2012-04-27 | 2021-03-02 | 日立化成诊断系统株式会社 | 样品中的测定对象成分的测定方法 |
| CN109337821B (zh) * | 2018-09-29 | 2022-06-24 | 中国海洋大学 | 一种产甾醇酯酶的枝孢菌及产酶方法 |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5218797B2 (en, 2012) * | 1973-03-10 | 1977-05-24 | ||
| JPS50142090A (en, 2012) * | 1974-04-30 | 1975-11-15 | ||
| JPS51129293A (en) * | 1975-05-02 | 1976-11-10 | Nippon Shoji Kk | Measurement method of activity of lecithin cholesteral acyl transferas e |
| JPS5217085A (en) * | 1975-07-30 | 1977-02-08 | Ono Pharmaceut Co Ltd | Method of quantitative determination of free fatty acids in serum usin g fatty acid activating enzymes |
| US4141792A (en) * | 1976-08-19 | 1979-02-27 | Hiroaki Hayashi | Composition and method for the quantitative determination of phospholipids |
| JPS5324888A (en) * | 1976-08-19 | 1978-03-08 | Kyowa Hakko Kogyo Kk | Composition for quantitating fats containing phosphorus |
| US4195126A (en) * | 1977-10-04 | 1980-03-25 | The Board Of Trustees Of The University Of Alabama | Albumin-dye complex for fatty acid determination |
| US4223090A (en) * | 1978-07-13 | 1980-09-16 | American Hospital Supply Corporation | Reagents for the enzymatic determination of triglycerides |
| US4242446A (en) * | 1978-07-26 | 1980-12-30 | Coulter Electronics, Inc. | Method for determining a substance in a biological fluid and reagent combination for use in the method |
| JPS55108297A (en) * | 1979-02-13 | 1980-08-20 | Toyobo Co Ltd | Determining method of free fatty acid |
-
1982
- 1982-06-24 FR FR8211073A patent/FR2508487B1/fr not_active Expired
- 1982-06-24 CA CA000405920A patent/CA1215903A/en not_active Expired
- 1982-06-24 IT IT22048/82A patent/IT1157281B/it active
- 1982-06-25 US US06/392,010 patent/US4491631A/en not_active Expired - Lifetime
- 1982-06-25 DE DE3249973A patent/DE3249973C2/de not_active Expired - Lifetime
- 1982-06-25 GB GB08218552A patent/GB2105843B/en not_active Expired
- 1982-06-25 CH CH3917/82A patent/CH664631A5/it not_active IP Right Cessation
- 1982-06-25 DE DE19823223874 patent/DE3223874A1/de active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| FR2508487B1 (fr) | 1986-06-27 |
| US4491631A (en) | 1985-01-01 |
| IT1157281B (it) | 1987-02-11 |
| DE3249973C2 (en, 2012) | 1990-02-15 |
| DE3223874C2 (en, 2012) | 1991-05-02 |
| GB2105843B (en) | 1984-11-07 |
| DE3223874A1 (de) | 1983-01-20 |
| GB2105843A (en) | 1983-03-30 |
| CH664631A5 (it) | 1988-03-15 |
| FR2508487A1 (fr) | 1982-12-31 |
| IT8222048A0 (it) | 1982-06-24 |
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