CA1068586A - .alpha.-AMYLASE ASSAY UTILIZING MODIFIED STARCH AS THE SUBSTRATE - Google Patents
.alpha.-AMYLASE ASSAY UTILIZING MODIFIED STARCH AS THE SUBSTRATEInfo
- Publication number
- CA1068586A CA1068586A CA281,158A CA281158A CA1068586A CA 1068586 A CA1068586 A CA 1068586A CA 281158 A CA281158 A CA 281158A CA 1068586 A CA1068586 A CA 1068586A
- Authority
- CA
- Canada
- Prior art keywords
- phosphate
- amylase
- glucose
- alpha
- sample
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 239000004382 Amylase Substances 0.000 title claims abstract description 31
- 239000000758 substrate Substances 0.000 title claims abstract description 19
- 229920000881 Modified starch Polymers 0.000 title claims abstract description 8
- 239000004368 Modified starch Substances 0.000 title claims abstract description 8
- 235000019426 modified starch Nutrition 0.000 title claims abstract description 8
- 238000003556 assay Methods 0.000 title description 11
- 238000000034 method Methods 0.000 claims abstract description 32
- 102000004190 Enzymes Human genes 0.000 claims abstract description 12
- 108090000790 Enzymes Proteins 0.000 claims abstract description 12
- 229930027945 nicotinamide-adenine dinucleotide Natural products 0.000 claims abstract description 11
- 238000005859 coupling reaction Methods 0.000 claims abstract description 9
- 210000002966 serum Anatomy 0.000 claims abstract description 9
- 102100031126 6-phosphogluconolactonase Human genes 0.000 claims abstract description 8
- 108010029731 6-phosphogluconolactonase Proteins 0.000 claims abstract description 8
- 108010018962 Glucosephosphate Dehydrogenase Proteins 0.000 claims abstract description 8
- BOPGDPNILDQYTO-NNYOXOHSSA-N nicotinamide-adenine dinucleotide Chemical compound C1=CCC(C(=O)N)=CN1[C@H]1[C@H](O)[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OC[C@@H]2[C@H]([C@@H](O)[C@@H](O2)N2C3=NC=NC(N)=C3N=C2)O)O1 BOPGDPNILDQYTO-NNYOXOHSSA-N 0.000 claims abstract description 8
- NBSCHQHZLSJFNQ-GASJEMHNSA-N D-Glucose 6-phosphate Chemical compound OC1O[C@H](COP(O)(O)=O)[C@@H](O)[C@H](O)[C@H]1O NBSCHQHZLSJFNQ-GASJEMHNSA-N 0.000 claims abstract description 7
- VFRROHXSMXFLSN-UHFFFAOYSA-N Glc6P Natural products OP(=O)(O)OCC(O)C(O)C(O)C(O)C=O VFRROHXSMXFLSN-UHFFFAOYSA-N 0.000 claims abstract description 7
- 102000009569 Phosphoglucomutase Human genes 0.000 claims abstract description 7
- 230000008878 coupling Effects 0.000 claims abstract description 7
- 238000010168 coupling process Methods 0.000 claims abstract description 7
- 108091000115 phosphomannomutase Proteins 0.000 claims abstract description 7
- 230000009471 action Effects 0.000 claims abstract description 4
- HXXFSFRBOHSIMQ-VFUOTHLCSA-N alpha-D-glucose 1-phosphate Chemical compound OC[C@H]1O[C@H](OP(O)(O)=O)[C@H](O)[C@@H](O)[C@@H]1O HXXFSFRBOHSIMQ-VFUOTHLCSA-N 0.000 claims abstract 2
- 229950010772 glucose-1-phosphate Drugs 0.000 claims abstract 2
- 239000003153 chemical reaction reagent Substances 0.000 claims description 17
- 108010073135 Phosphorylases Proteins 0.000 claims description 14
- 102000009097 Phosphorylases Human genes 0.000 claims description 14
- UDMBCSSLTHHNCD-UHFFFAOYSA-N Coenzym Q(11) Natural products C1=NC=2C(N)=NC=NC=2N1C1OC(COP(O)(O)=O)C(O)C1O UDMBCSSLTHHNCD-UHFFFAOYSA-N 0.000 claims description 6
- BAWFJGJZGIEFAR-NNYOXOHSSA-N NAD zwitterion Chemical compound NC(=O)C1=CC=C[N+]([C@H]2[C@@H]([C@H](O)[C@@H](COP([O-])(=O)OP(O)(=O)OC[C@@H]3[C@H]([C@@H](O)[C@@H](O3)N3C4=NC=NC(N)=C4N=C3)O)O2)O)=C1 BAWFJGJZGIEFAR-NNYOXOHSSA-N 0.000 claims description 6
- UDMBCSSLTHHNCD-KQYNXXCUSA-N adenosine 5'-monophosphate Chemical group C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP(O)(O)=O)[C@@H](O)[C@H]1O UDMBCSSLTHHNCD-KQYNXXCUSA-N 0.000 claims description 6
- 229950006790 adenosine phosphate Drugs 0.000 claims description 6
- 150000004676 glycans Chemical class 0.000 claims description 6
- 229920001282 polysaccharide Polymers 0.000 claims description 6
- 239000005017 polysaccharide Substances 0.000 claims description 6
- RXGJTUSBYWCRBK-UHFFFAOYSA-M 5-methylphenazinium methyl sulfate Chemical compound COS([O-])(=O)=O.C1=CC=C2[N+](C)=C(C=CC=C3)C3=NC2=C1 RXGJTUSBYWCRBK-UHFFFAOYSA-M 0.000 claims description 3
- 239000012992 electron transfer agent Substances 0.000 claims description 3
- 230000000813 microbial effect Effects 0.000 claims description 3
- BIRSGZKFKXLSJQ-SQOUGZDYSA-N 6-Phospho-D-gluconate Chemical compound OP(=O)(O)OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C(O)=O BIRSGZKFKXLSJQ-SQOUGZDYSA-N 0.000 claims description 2
- 241000192132 Leuconostoc Species 0.000 claims description 2
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 2
- 239000013060 biological fluid Substances 0.000 claims description 2
- 238000005259 measurement Methods 0.000 claims description 2
- XJLXINKUBYWONI-NNYOXOHSSA-O NADP(+) Chemical compound NC(=O)C1=CC=C[N+]([C@H]2[C@@H]([C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OC[C@@H]3[C@H]([C@@H](OP(O)(O)=O)[C@@H](O3)N3C4=NC=NC(N)=C4N=C3)O)O2)O)=C1 XJLXINKUBYWONI-NNYOXOHSSA-O 0.000 claims 2
- JJMQRJKPLUACSO-UHFFFAOYSA-N 3-(4-iodophenyl)-2-(4-nitrophenyl)-5-phenyl-1,3-dihydrotetrazol-3-ium;chloride Chemical group [Cl-].C1=CC([N+](=O)[O-])=CC=C1N1N(C=2C=CC(I)=CC=2)[NH2+]C(C=2C=CC=CC=2)=N1 JJMQRJKPLUACSO-UHFFFAOYSA-N 0.000 claims 1
- ACFIXJIJDZMPPO-NNYOXOHSSA-N NADPH Chemical compound C1=CCC(C(=O)N)=CN1[C@H]1[C@H](O)[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OC[C@@H]2[C@H]([C@@H](OP(O)(O)=O)[C@@H](O2)N2C3=NC=NC(N)=C3N=C2)O)O1 ACFIXJIJDZMPPO-NNYOXOHSSA-N 0.000 claims 1
- 229910019142 PO4 Inorganic materials 0.000 claims 1
- 239000012190 activator Substances 0.000 claims 1
- 239000007788 liquid Substances 0.000 claims 1
- 229940101270 nicotinamide adenine dinucleotide (nad) Drugs 0.000 claims 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 claims 1
- 239000010452 phosphate Substances 0.000 claims 1
- 239000007787 solid Substances 0.000 claims 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims 1
- 229920002472 Starch Polymers 0.000 abstract description 16
- 235000019698 starch Nutrition 0.000 abstract description 16
- 239000008107 starch Substances 0.000 abstract description 15
- 238000006243 chemical reaction Methods 0.000 abstract description 10
- 230000015572 biosynthetic process Effects 0.000 abstract description 5
- 230000000694 effects Effects 0.000 abstract description 5
- 238000002835 absorbance Methods 0.000 abstract description 4
- 210000002700 urine Anatomy 0.000 abstract description 4
- 238000003776 cleavage reaction Methods 0.000 abstract description 2
- 238000003759 clinical diagnosis Methods 0.000 abstract description 2
- 229910052816 inorganic phosphate Inorganic materials 0.000 abstract description 2
- 230000007017 scission Effects 0.000 abstract description 2
- 229950006238 nadide Drugs 0.000 description 5
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 4
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
- HXXFSFRBOHSIMQ-UHFFFAOYSA-N [3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl] dihydrogen phosphate Chemical compound OCC1OC(OP(O)(O)=O)C(O)C(O)C1O HXXFSFRBOHSIMQ-UHFFFAOYSA-N 0.000 description 4
- 239000008103 glucose Substances 0.000 description 4
- 238000004737 colorimetric analysis Methods 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 230000003647 oxidation Effects 0.000 description 3
- 238000007254 oxidation reaction Methods 0.000 description 3
- 230000035945 sensitivity Effects 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- XTUZNJKZDCNDAP-UHFFFAOYSA-N 1-[2-(4-iodophenyl)-3-nitrophenyl]-5-phenyl-2H-tetrazol-1-ium chloride Chemical compound [Cl-].[O-][N+](=O)C1=CC=CC([N+]2=C(N=NN2)C=2C=CC=CC=2)=C1C1=CC=C(I)C=C1 XTUZNJKZDCNDAP-UHFFFAOYSA-N 0.000 description 2
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 description 2
- 229920000945 Amylopectin Polymers 0.000 description 2
- 108091006149 Electron carriers Proteins 0.000 description 2
- 108010073178 Glucan 1,4-alpha-Glucosidase Proteins 0.000 description 2
- 102100022624 Glucoamylase Human genes 0.000 description 2
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 description 2
- 241000283973 Oryctolagus cuniculus Species 0.000 description 2
- 108010065081 Phosphorylase b Proteins 0.000 description 2
- 239000001177 diphosphate Substances 0.000 description 2
- 239000004615 ingredient Substances 0.000 description 2
- 210000003205 muscle Anatomy 0.000 description 2
- 239000008363 phosphate buffer Substances 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- 229920000856 Amylose Polymers 0.000 description 1
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 1
- 101710088194 Dehydrogenase Proteins 0.000 description 1
- 229920001353 Dextrin Polymers 0.000 description 1
- 239000004375 Dextrin Substances 0.000 description 1
- 102000028526 Dihydrolipoamide Dehydrogenase Human genes 0.000 description 1
- 108010028127 Dihydrolipoamide Dehydrogenase Proteins 0.000 description 1
- 229920001503 Glucan Polymers 0.000 description 1
- 229920002527 Glycogen Polymers 0.000 description 1
- 229920002774 Maltodextrin Polymers 0.000 description 1
- 239000005913 Maltodextrin Substances 0.000 description 1
- 102100026367 Pancreatic alpha-amylase Human genes 0.000 description 1
- 108010065084 Phosphorylase a Proteins 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 238000007398 colorimetric assay Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 235000019425 dextrin Nutrition 0.000 description 1
- XPPKVPWEQAFLFU-UHFFFAOYSA-J diphosphate(4-) Chemical compound [O-]P([O-])(=O)OP([O-])([O-])=O XPPKVPWEQAFLFU-UHFFFAOYSA-J 0.000 description 1
- 235000011180 diphosphates Nutrition 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- -1 glucose-l-phosphate Glucose-l-phosphate phospho Chemical class 0.000 description 1
- 229940096919 glycogen Drugs 0.000 description 1
- 230000003301 hydrolyzing effect Effects 0.000 description 1
- 229910052740 iodine Inorganic materials 0.000 description 1
- 239000011630 iodine Substances 0.000 description 1
- 229940035034 maltodextrin Drugs 0.000 description 1
- 108010085781 maltodextrin phosphorylase Proteins 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 229920001542 oligosaccharide Polymers 0.000 description 1
- 150000002482 oligosaccharides Chemical class 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 210000000496 pancreas Anatomy 0.000 description 1
- KHIWWQKSHDUIBK-UHFFFAOYSA-N periodic acid Chemical compound OI(=O)(=O)=O KHIWWQKSHDUIBK-UHFFFAOYSA-N 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 239000012279 sodium borohydride Substances 0.000 description 1
- 229910000033 sodium borohydride Inorganic materials 0.000 description 1
- 235000000346 sugar Nutrition 0.000 description 1
- 150000008163 sugars Chemical class 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 125000003831 tetrazolyl group Chemical group 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
- C12Q1/40—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving amylase
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US70177876A | 1976-07-01 | 1976-07-01 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA1068586A true CA1068586A (en) | 1979-12-25 |
Family
ID=24818638
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA281,158A Expired CA1068586A (en) | 1976-07-01 | 1977-06-22 | .alpha.-AMYLASE ASSAY UTILIZING MODIFIED STARCH AS THE SUBSTRATE |
Country Status (5)
| Country | Link |
|---|---|
| JP (1) | JPS533891A (enrdf_load_stackoverflow) |
| CA (1) | CA1068586A (enrdf_load_stackoverflow) |
| DE (1) | DE2729636B2 (enrdf_load_stackoverflow) |
| FR (1) | FR2356939A1 (enrdf_load_stackoverflow) |
| GB (1) | GB1543130A (enrdf_load_stackoverflow) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0048035B1 (en) * | 1978-06-06 | 1983-12-07 | Flow General, Inc. | Maltodextrin phosphorylase limit dextrin and method of producing it |
| US4304854A (en) | 1978-06-06 | 1981-12-08 | Worthington Biochemical Corporation | Alpha-amylase assay and substrates for use therein |
| JPS5768798A (en) * | 1980-10-14 | 1982-04-27 | Toyo Jozo Co Ltd | Novel measurement of amylase activity |
| US4649108A (en) * | 1984-07-26 | 1987-03-10 | Genzyme Corporation | Alpha amylase assay |
| US5043436A (en) * | 1986-11-20 | 1991-08-27 | Kurita Water Ind., Ltd. | Substrate for measurement of alpha-amylase activity |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB1366342A (en) * | 1970-07-28 | 1974-09-11 | Hayashibara Co | Process for the preparation of amylose |
| US3879263A (en) * | 1973-09-06 | 1975-04-22 | Du Pont | Method for the determination of amylase |
| US4097336A (en) * | 1976-02-13 | 1978-06-27 | Beckman Instruments, Inc. | Reagent system for beta-amylase assay |
-
1977
- 1977-06-22 CA CA281,158A patent/CA1068586A/en not_active Expired
- 1977-06-29 JP JP7668477A patent/JPS533891A/ja active Granted
- 1977-06-30 GB GB2751577A patent/GB1543130A/en not_active Expired
- 1977-06-30 FR FR7720191A patent/FR2356939A1/fr active Granted
- 1977-06-30 DE DE19772729636 patent/DE2729636B2/de not_active Ceased
Also Published As
| Publication number | Publication date |
|---|---|
| JPS5639637B2 (enrdf_load_stackoverflow) | 1981-09-14 |
| FR2356939A1 (fr) | 1978-01-27 |
| DE2729636A1 (de) | 1978-02-02 |
| FR2356939B1 (enrdf_load_stackoverflow) | 1983-11-25 |
| GB1543130A (en) | 1979-03-28 |
| JPS533891A (en) | 1978-01-13 |
| DE2729636B2 (de) | 1979-03-15 |
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