CA1068508A - Fluorescence spectrophotometer - Google Patents
Fluorescence spectrophotometerInfo
- Publication number
- CA1068508A CA1068508A CA267,617A CA267617A CA1068508A CA 1068508 A CA1068508 A CA 1068508A CA 267617 A CA267617 A CA 267617A CA 1068508 A CA1068508 A CA 1068508A
- Authority
- CA
- Canada
- Prior art keywords
- emission
- excitation
- sample
- radiation
- slit
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 230000005284 excitation Effects 0.000 claims abstract description 167
- 230000003287 optical effect Effects 0.000 claims abstract description 67
- 230000005855 radiation Effects 0.000 claims description 79
- 206010001497 Agitation Diseases 0.000 claims 1
- 230000000875 corresponding effect Effects 0.000 description 12
- 230000000694 effects Effects 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 230000009467 reduction Effects 0.000 description 4
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N silicon dioxide Inorganic materials O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 4
- 238000001228 spectrum Methods 0.000 description 4
- 230000004075 alteration Effects 0.000 description 3
- 201000009310 astigmatism Diseases 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
- 230000001186 cumulative effect Effects 0.000 description 3
- 238000001514 detection method Methods 0.000 description 3
- 230000006872 improvement Effects 0.000 description 3
- 229910052724 xenon Inorganic materials 0.000 description 3
- FHNFHKCVQCLJFQ-UHFFFAOYSA-N xenon atom Chemical compound [Xe] FHNFHKCVQCLJFQ-UHFFFAOYSA-N 0.000 description 3
- 239000012141 concentrate Substances 0.000 description 2
- 238000010276 construction Methods 0.000 description 2
- 230000014509 gene expression Effects 0.000 description 2
- 238000005286 illumination Methods 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 230000005499 meniscus Effects 0.000 description 2
- 239000010453 quartz Substances 0.000 description 2
- 230000035945 sensitivity Effects 0.000 description 2
- 230000009471 action Effects 0.000 description 1
- 230000002730 additional effect Effects 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 235000008504 concentrate Nutrition 0.000 description 1
- 238000000295 emission spectrum Methods 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 238000000695 excitation spectrum Methods 0.000 description 1
- 238000002189 fluorescence spectrum Methods 0.000 description 1
- 238000003384 imaging method Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- PYWVYCXTNDRMGF-UHFFFAOYSA-N rhodamine B Chemical compound [Cl-].C=12C=CC(=[N+](CC)CC)C=C2OC2=CC(N(CC)CC)=CC=C2C=1C1=CC=CC=C1C(O)=O PYWVYCXTNDRMGF-UHFFFAOYSA-N 0.000 description 1
- 229940043267 rhodamine b Drugs 0.000 description 1
- 239000000377 silicon dioxide Substances 0.000 description 1
- 238000001429 visible spectrum Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
- G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
- G01J3/28—Investigating the spectrum
- G01J3/44—Raman spectrometry; Scattering spectrometry ; Fluorescence spectrometry
- G01J3/4406—Fluorescence spectrometry
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
Landscapes
- Physics & Mathematics (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Analytical Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Spectrometry And Color Measurement (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US05/639,804 US4022529A (en) | 1975-12-11 | 1975-12-11 | Feature extraction system for extracting a predetermined feature from a signal |
| US05/731,765 US4099872A (en) | 1975-12-11 | 1976-10-12 | Fluorescence spectrophotometer |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA1068508A true CA1068508A (en) | 1979-12-25 |
Family
ID=27093401
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA267,617A Expired CA1068508A (en) | 1975-12-11 | 1976-12-10 | Fluorescence spectrophotometer |
Country Status (3)
| Country | Link |
|---|---|
| JP (1) | JPS5919305B2 (ja) |
| CA (1) | CA1068508A (ja) |
| DE (1) | DE2656417C2 (ja) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE2938056C2 (de) * | 1979-09-20 | 1986-12-11 | Gesellschaft für Strahlen- und Umweltforschung mbH, 8000 München | Vorrichtung zur fluorometrischen Untersuchung von Proben |
| JPS5661633A (en) * | 1979-10-24 | 1981-05-27 | Hitachi Ltd | Densitometer for measuring secondary light of developed constituent |
| US4311387A (en) * | 1979-12-14 | 1982-01-19 | The Perkin-Elmer Corporation | Flow-through sample cell and collecting and illuminating optics for liquid chromatography |
| DE3215249C2 (de) * | 1982-04-23 | 1984-10-11 | Bodenseewerk Perkin-Elmer & Co GmbH, 7770 Überlingen | Photometer zur Messung der Atomfluoreszenz |
| JPS6021955U (ja) * | 1983-03-28 | 1985-02-15 | 株式会社島津製作所 | クロスイルミネ−シヨン用試料セルホルダ |
| DE4344235C2 (de) * | 1993-12-23 | 1997-01-30 | Aero Laser Ges Fuer Gasanalyti | Vorrichtung zur Messung der Konzentration einer Substanz, insbesondere Peroxid, in einer gasförmigen oder flüssigen Probe mittels enzymatischer Fluoreszenz |
| AT502194B1 (de) * | 2005-12-16 | 2007-02-15 | Joanneum Res Forschungsgmbh | Verfahren und vorrichtung zur bestimmung der fluoreszenz einer probe sowie verwendung derselben |
| DE102018129010B4 (de) * | 2018-11-19 | 2023-09-28 | Spectro Analytical Instruments Gmbh | Anordnung zur optischen Emissionsspektrometrie mit verbesserter Lichtausbeute |
| DE102019201440A1 (de) | 2019-02-05 | 2020-08-06 | Implen GmbH | Vorrichtung für eine lichtspektroskopische Analyse |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU6306973A (en) * | 1972-12-05 | 1975-06-05 | White J U | Radiation measuring apparatus |
| JPS545988B2 (ja) * | 1973-04-16 | 1979-03-23 |
-
1976
- 1976-12-10 CA CA267,617A patent/CA1068508A/en not_active Expired
- 1976-12-11 JP JP51149298A patent/JPS5919305B2/ja not_active Expired
- 1976-12-13 DE DE2656417A patent/DE2656417C2/de not_active Expired
Also Published As
| Publication number | Publication date |
|---|---|
| JPS5919305B2 (ja) | 1984-05-04 |
| JPS5292583A (en) | 1977-08-04 |
| DE2656417C2 (de) | 1986-06-26 |
| DE2656417A1 (de) | 1977-07-07 |
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