BRPI0821747A8 - Método, ensaio imunocromatográfico e fita de teste imunocromatográfica para detecção do complexo de mycobacterium tuberculosis - Google Patents
Método, ensaio imunocromatográfico e fita de teste imunocromatográfica para detecção do complexo de mycobacterium tuberculosisInfo
- Publication number
- BRPI0821747A8 BRPI0821747A8 BRPI0821747A BRPI0821747A BRPI0821747A8 BR PI0821747 A8 BRPI0821747 A8 BR PI0821747A8 BR PI0821747 A BRPI0821747 A BR PI0821747A BR PI0821747 A BRPI0821747 A BR PI0821747A BR PI0821747 A8 BRPI0821747 A8 BR PI0821747A8
- Authority
- BR
- Brazil
- Prior art keywords
- mycobacterium tuberculosis
- immunochromatographic
- detection
- tuberculosis complex
- assay
- Prior art date
Links
- 241001302239 Mycobacterium tuberculosis complex Species 0.000 title abstract 3
- 238000003556 assay Methods 0.000 title abstract 3
- 238000001514 detection method Methods 0.000 title abstract 2
- 238000000034 method Methods 0.000 title abstract 2
- 238000003018 immunoassay Methods 0.000 abstract 3
- 101100291912 Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97) mpb64 gene Proteins 0.000 abstract 2
- 241000187479 Mycobacterium tuberculosis Species 0.000 abstract 2
- 239000012472 biological sample Substances 0.000 abstract 2
- 125000003275 alpha amino acid group Chemical group 0.000 abstract 1
- 239000006185 dispersion Substances 0.000 abstract 1
- 230000002779 inactivation Effects 0.000 abstract 1
- 208000015181 infectious disease Diseases 0.000 abstract 1
- 239000000523 sample Substances 0.000 abstract 1
- 230000007928 solubilization Effects 0.000 abstract 1
- 238000005063 solubilization Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/12—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from bacteria
- C07K16/1267—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from bacteria from Gram-positive bacteria
- C07K16/1289—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from bacteria from Gram-positive bacteria from Mycobacteriaceae (F)
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56911—Bacteria
- G01N33/5695—Mycobacteria
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Engineering & Computer Science (AREA)
- Urology & Nephrology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Medicinal Chemistry (AREA)
- Hematology (AREA)
- Organic Chemistry (AREA)
- Biomedical Technology (AREA)
- Genetics & Genomics (AREA)
- Biophysics (AREA)
- Tropical Medicine & Parasitology (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Microbiology (AREA)
- Virology (AREA)
- Food Science & Technology (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
MÉTODO, ENSAIO IMUNOCROMATOGRÁFICO E FITA DE TESTE IMUNOCROMATOGRÁFICA PARA DETECÇÃO DO COMPLEXO DE MYCOBACTERIUM TUBERCULOSIS A presente invenção refere-se a um método para detectar especificamente um antígeno da proteína MTP64 secretória complexoespecífica de Mycobacterium tuberculosis em uma amostra biológica, por meio do qual o diagnóstico da infecção com Mycobacterium tuberculosis é realizado rápida e seguramente com precisão mais alta que antes. Um anticorpo que reconhece um epitopo para a MPB64 localizado em qualquer uma das sequências de aminoácidos SEQ ID NOS: 2 a 4, particularmente um anticorpo monoclonal foi obtido. Deste modo, um imunoensaio utilizando o anticorpo, particularmente um imunoensaio do tipo sanduiche utilizando os primeiro e segundo anticorpos para a MPB64, particularmente um ensaio imunocromatográfico e uma fita de teste imunocromatográfica são fornecidos. Uma amostra biológica pode ser rapidamente submetida ao imunoensaio sem ficar em cultura ou após cultura por um período de tempo antes da bactéria do complexo Mycobacterium tuberculosis na amostra começar a crescer substancialmente. A amostra biológica pode ser pré-tratada através do tratamento para inativação de Mycobacterium tuberculosis, ou tratamento por dispersão ou solubilização.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2007-339376 | 2007-12-28 | ||
JP2007339376 | 2007-12-28 | ||
PCT/JP2008/073200 WO2009084481A1 (ja) | 2007-12-28 | 2008-12-19 | 結核菌群の免疫検出法 |
Publications (4)
Publication Number | Publication Date |
---|---|
BRPI0821747A2 BRPI0821747A2 (pt) | 2015-06-16 |
BRPI0821747A8 true BRPI0821747A8 (pt) | 2017-10-03 |
BRPI0821747B1 BRPI0821747B1 (pt) | 2019-04-30 |
BRPI0821747B8 BRPI0821747B8 (pt) | 2021-07-27 |
Family
ID=40824198
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
BRPI0821747A BRPI0821747B8 (pt) | 2007-12-28 | 2008-12-19 | método, ensaio imunocromatográfico e fita de teste imunocromatográfica para detecção do complexo de mycobacterium tuberculosis |
Country Status (8)
Country | Link |
---|---|
US (1) | US8541179B2 (pt) |
EP (1) | EP2226635B1 (pt) |
JP (1) | JP4943515B2 (pt) |
CN (3) | CN103123354B (pt) |
BR (1) | BRPI0821747B8 (pt) |
HK (1) | HK1152375A1 (pt) |
RU (1) | RU2473095C2 (pt) |
WO (1) | WO2009084481A1 (pt) |
Families Citing this family (15)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2012088577A2 (pt) * | 2010-12-29 | 2012-07-05 | Universidade Federal De Minas Gerais - Ufmg | Proteína recombinante de mycobacterium sp., teste imunodiagnóstico e vacina para tuberculose |
ES2746565T3 (es) | 2012-04-05 | 2020-03-06 | Tauns Co Ltd | Procedimiento y kit para la detección inmunológica de complejo de Mycobacterium tuberculosis |
US8735091B2 (en) * | 2012-05-17 | 2014-05-27 | Biomerieux, Inc. | Methods for inactivation and extraction of acid-fast bacteria for characterization and/or identification using mass spectrometry |
JP6481192B2 (ja) * | 2013-12-27 | 2019-03-13 | 地方独立行政法人大阪健康安全基盤研究所 | クドア・セプテンプンクタータの迅速検出法 |
RU2594063C1 (ru) * | 2015-04-27 | 2016-08-10 | Федеральное государственное бюджетное научное учреждение "Центральный научно-исследовательский институт туберкулёза" | Способ диагностики микобактерий туберкулёза |
CN106526171A (zh) * | 2015-09-11 | 2017-03-22 | 睿嘉生物科技股份有限公司 | 免疫检测套组 |
EP3225310A1 (fr) | 2016-03-31 | 2017-10-04 | Biomérieux | Membranes d'analyse de dispositifs microfluidiques, réalisées en un matériau en fibre de verre |
RU2645907C1 (ru) * | 2016-09-07 | 2018-02-28 | Общество с ограниченной ответственностью "Рикомед" | Вязкость среды как инструмент контроля предела обнаружения иммунохроматографических тест-систем |
US20210072235A1 (en) * | 2017-12-22 | 2021-03-11 | Sugentech, Inc. | Method for diagnosing tuberculosis |
CN108318694A (zh) * | 2018-01-29 | 2018-07-24 | 厦门大学 | 一种采用免疫试纸条对抗原进行定量检测的方法 |
JP7403948B2 (ja) * | 2018-10-31 | 2023-12-25 | 公益財団法人筑波メディカルセンター | 試料の前処理方法 |
JP7395254B2 (ja) * | 2019-02-25 | 2023-12-11 | 公益財団法人筑波メディカルセンター | 微生物の検出方法 |
CN113789283A (zh) * | 2021-10-20 | 2021-12-14 | 周玉美 | 一种快速培养结核分枝杆菌的培养基及其制备方法和应用 |
CN114813671B (zh) * | 2022-04-06 | 2023-07-14 | 四川大学华西医院 | 基于金属离子和其纳米粒子选择性调控QDs和钙黄绿素荧光信号的分析方法及应用 |
CN114805586A (zh) * | 2022-04-14 | 2022-07-29 | 天津华科泰生物技术有限公司 | 抗MxA单克隆抗体组合物及其应用 |
Family Cites Families (15)
Publication number | Priority date | Publication date | Assignee | Title |
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JP2624759B2 (ja) * | 1988-03-30 | 1997-06-25 | 味の素株式会社 | Bcg菌由来のmpb64蛋白及びその製造法 |
JPH07110332A (ja) | 1993-10-12 | 1995-04-25 | Tetsuo Tomiyama | 結核菌の検査試薬及び検出方法 |
US5658749A (en) * | 1994-04-05 | 1997-08-19 | Corning Clinical Laboratories, Inc. | Method for processing mycobacteria |
WO1997009429A2 (en) * | 1995-09-01 | 1997-03-13 | Corixa Corporation | Compounds and methods for diagnosis of tuberculosis |
US6458366B1 (en) | 1995-09-01 | 2002-10-01 | Corixa Corporation | Compounds and methods for diagnosis of tuberculosis |
US6416962B1 (en) * | 1995-11-20 | 2002-07-09 | Kreatech Biotechnology B.V. | Method and device for identifying a mycobacterium species responsible for a mycobacterial infection |
JP4269006B2 (ja) | 1997-09-30 | 2009-05-27 | 株式会社ビーエル | 結核菌検出法 |
JP2001247094A (ja) * | 2000-03-06 | 2001-09-11 | Hisao Watanabe | 熱風船 |
WO2002095411A1 (en) * | 2001-05-18 | 2002-11-28 | Virogates Aps | A method of diagnosing or prognosticating major respiratory bacterial pathogens in a subject |
CN1425775A (zh) * | 2001-12-11 | 2003-06-25 | 李克生 | 结核抗体金标测试条及其制备方法 |
CN1312723C (zh) * | 2003-06-18 | 2007-04-25 | 友达光电股份有限公司 | 等离子发光面板 |
JP2007316068A (ja) | 2006-05-22 | 2007-12-06 | Polaris Wireless Inc | 無線端末の居場所の予測方法 |
CN100494352C (zh) * | 2006-12-30 | 2009-06-03 | 华中农业大学 | 检测牛结核抗体的免疫胶体金试纸条及制备方法 |
JP4406656B2 (ja) * | 2007-07-13 | 2010-02-03 | 株式会社ビーエル | 結核菌検出法 |
CN101294964B (zh) | 2007-11-27 | 2012-06-27 | 复旦大学附属华山医院 | 一种检测活动性结核病和结核潜伏感染的试剂和方法 |
-
2008
- 2008-12-19 US US12/810,217 patent/US8541179B2/en active Active
- 2008-12-19 CN CN201210521148.0A patent/CN103123354B/zh active Active
- 2008-12-19 BR BRPI0821747A patent/BRPI0821747B8/pt active IP Right Grant
- 2008-12-19 EP EP08866382A patent/EP2226635B1/en active Active
- 2008-12-19 CN CN201210519680.9A patent/CN103123353B/zh active Active
- 2008-12-19 CN CN2008801256855A patent/CN101925819B/zh active Active
- 2008-12-19 WO PCT/JP2008/073200 patent/WO2009084481A1/ja active Application Filing
- 2008-12-19 JP JP2009548016A patent/JP4943515B2/ja active Active
- 2008-12-19 RU RU2010131460/15A patent/RU2473095C2/ru active
-
2011
- 2011-06-17 HK HK11106232.2A patent/HK1152375A1/xx unknown
Also Published As
Publication number | Publication date |
---|---|
EP2226635A1 (en) | 2010-09-08 |
WO2009084481A1 (ja) | 2009-07-09 |
HK1152375A1 (en) | 2012-02-24 |
RU2473095C2 (ru) | 2013-01-20 |
CN103123353B (zh) | 2014-11-12 |
BRPI0821747B8 (pt) | 2021-07-27 |
CN101925819B (zh) | 2013-06-12 |
BRPI0821747A2 (pt) | 2015-06-16 |
CN103123354A (zh) | 2013-05-29 |
CN103123353A (zh) | 2013-05-29 |
US20100285506A1 (en) | 2010-11-11 |
EP2226635A4 (en) | 2012-07-04 |
EP2226635B1 (en) | 2013-03-13 |
JP4943515B2 (ja) | 2012-05-30 |
BRPI0821747B1 (pt) | 2019-04-30 |
JPWO2009084481A1 (ja) | 2011-05-19 |
US8541179B2 (en) | 2013-09-24 |
CN101925819A (zh) | 2010-12-22 |
RU2010131460A (ru) | 2012-02-10 |
CN103123354B (zh) | 2014-11-12 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
B25A | Requested transfer of rights approved |
Owner name: TAUNS CO., LTD. (JP) |
|
B06F | Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette] | ||
B06I | Publication of requirement cancelled [chapter 6.9 patent gazette] | ||
B06A | Patent application procedure suspended [chapter 6.1 patent gazette] | ||
B06A | Patent application procedure suspended [chapter 6.1 patent gazette] | ||
B09A | Decision: intention to grant [chapter 9.1 patent gazette] | ||
B16A | Patent or certificate of addition of invention granted [chapter 16.1 patent gazette] |
Free format text: PRAZO DE VALIDADE: 10 (DEZ) ANOS CONTADOS A PARTIR DE 30/04/2019, OBSERVADAS AS CONDICOES LEGAIS. (CO) 10 (DEZ) ANOS CONTADOS A PARTIR DE 30/04/2019, OBSERVADAS AS CONDICOES LEGAIS |
|
B16C | Correction of notification of the grant [chapter 16.3 patent gazette] |
Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 19/12/2008, OBSERVADAS AS CONDICOES LEGAIS. PATENTE CONCEDIDA CONFORME ADI 5.529/DF, QUE DETERMINA A ALTERACAO DO PRAZO DE CONCESSAO |