BRPI0818273A2 - Sequências sinalizadoras e chaperonas coexpressas para aprimoramento de produção de proteína em uma célula hospedeira. - Google Patents

Sequências sinalizadoras e chaperonas coexpressas para aprimoramento de produção de proteína em uma célula hospedeira.

Info

Publication number
BRPI0818273A2
BRPI0818273A2 BRPI0818273-6A2A BRPI0818273A BRPI0818273A2 BR PI0818273 A2 BRPI0818273 A2 BR PI0818273A2 BR PI0818273 A BRPI0818273 A BR PI0818273A BR PI0818273 A2 BRPI0818273 A2 BR PI0818273A2
Authority
BR
Brazil
Prior art keywords
chaperonas
coexpress
improvement
host cell
protein production
Prior art date
Application number
BRPI0818273-6A2A
Other languages
English (en)
Portuguese (pt)
Inventor
Kai Bao
Huaming Wang
Original Assignee
Danisco Us Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Danisco Us Inc filed Critical Danisco Us Inc
Publication of BRPI0818273A2 publication Critical patent/BRPI0818273A2/pt

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2428—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0055—Oxidoreductases (1.) acting on diphenols and related substances as donors (1.10)
    • C12N9/0057—Oxidoreductases (1.) acting on diphenols and related substances as donors (1.10) with oxygen as acceptor (1.10.3)
    • C12N9/0061—Laccase (1.10.3.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y110/00—Oxidoreductases acting on diphenols and related substances as donors (1.10)
    • C12Y110/03—Oxidoreductases acting on diphenols and related substances as donors (1.10) with an oxygen as acceptor (1.10.3)
    • C12Y110/03002—Laccase (1.10.3.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01091—Cellulose 1,4-beta-cellobiosidase (3.2.1.91)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Mycology (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
BRPI0818273-6A2A 2007-11-01 2008-10-30 Sequências sinalizadoras e chaperonas coexpressas para aprimoramento de produção de proteína em uma célula hospedeira. BRPI0818273A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US98443007P 2007-11-01 2007-11-01
PCT/US2008/081721 WO2009058956A1 (en) 2007-11-01 2008-10-30 Signal sequences and co-expressed chaperones for improving protein production in a host cell

Publications (1)

Publication Number Publication Date
BRPI0818273A2 true BRPI0818273A2 (pt) 2014-10-14

Family

ID=40304979

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0818273-6A2A BRPI0818273A2 (pt) 2007-11-01 2008-10-30 Sequências sinalizadoras e chaperonas coexpressas para aprimoramento de produção de proteína em uma célula hospedeira.

Country Status (10)

Country Link
US (1) US20090221030A1 (cg-RX-API-DMAC7.html)
EP (1) EP2203556A1 (cg-RX-API-DMAC7.html)
JP (3) JP5252746B2 (cg-RX-API-DMAC7.html)
CN (1) CN101842479A (cg-RX-API-DMAC7.html)
AU (1) AU2008318644B2 (cg-RX-API-DMAC7.html)
BR (1) BRPI0818273A2 (cg-RX-API-DMAC7.html)
CA (1) CA2704548A1 (cg-RX-API-DMAC7.html)
CO (1) CO6210760A2 (cg-RX-API-DMAC7.html)
MX (1) MX2010004325A (cg-RX-API-DMAC7.html)
WO (1) WO2009058956A1 (cg-RX-API-DMAC7.html)

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KR20110117064A (ko) 2008-12-24 2011-10-26 다니스코 유에스 인크. 라카아제 및 저온에서의 이의 사용 방법
US8883485B2 (en) 2009-03-03 2014-11-11 Danisco Us Inc. Oxidative decolorization of dyes with enzymatically generated peracid method, composition and kit of parts
FI20095615A0 (fi) * 2009-06-02 2009-06-02 Oulun Yliopisto Menetelmä luonnollisesti laskostuneiden proteiinien tuottamiseksi prokaryootti-isännässä
US20130269118A1 (en) 2010-10-18 2013-10-17 Danisco Us Inc. Local color modification of dyed fabrics using a laccase system
WO2012138474A1 (en) 2011-04-06 2012-10-11 Danisco Us Inc. Laccase variants having increased expression and/or activity
JP2012235767A (ja) * 2011-04-27 2012-12-06 Toyota Motor Corp 変異トリコデルマ属微生物及びこれを用いたタンパク質の製造方法
EP3358000A3 (en) 2012-01-05 2018-12-19 Glykos Finland Oy Protease deficient filamentous fungal cells and methods of use thereof
WO2013111754A1 (ja) * 2012-01-23 2013-08-01 旭硝子株式会社 発現ベクターおよび蛋白質の製造方法
DK2852610T3 (en) 2012-05-23 2018-09-03 Glykos Finland Oy PRODUCTION OF FUCOSYLED GLYCOPROTEIN
US9993534B2 (en) * 2013-03-12 2018-06-12 Wisconsin Alumni Research Foundation Method of treating fungal infection
WO2015004241A2 (en) 2013-07-10 2015-01-15 Novartis Ag Multiple proteases deficient filamentous fungal cells and methods of use thereof
CN104694560A (zh) * 2013-12-04 2015-06-10 中国科学院天津工业生物技术研究所 一种源于里氏木霉的二硫键异构酶基因Trpdi2及其用途
JP2017511147A (ja) 2014-04-17 2017-04-20 ベーリンガー インゲルハイム エルツェーファウ ゲゼルシャフト ミット ベシュレンクテル ハフツング ウント コンパニー コマンディトゲゼルシャフト 目的タンパク質を発現させるための組換え宿主細胞
AU2015248815B2 (en) 2014-04-17 2021-07-08 Boehringer Ingelheim Rcv Gmbh & Co Kg Recombinant host cell engineered to overexpress helper proteins
WO2016012468A1 (en) 2014-07-21 2016-01-28 Novartis Ag Production of glycoproteins with mammalian-like n-glycans in filamentous fungi
US20170233746A1 (en) * 2014-08-15 2017-08-17 Danisco Us Inc. Compositions and methods for improved protein production
US10526609B2 (en) * 2016-08-01 2020-01-07 Aduro Biotech, Inc. Protein expression enhancer sequences and use thereof
WO2020002494A1 (en) * 2018-06-27 2020-01-02 Boehringer Ingelheim Rcv Gmbh & Co Kg Means and methods for increased protein expression by use of transcription factors
KR102138272B1 (ko) * 2018-09-19 2020-07-28 주식회사 바이오앱 BiP 단편을 포함하는 재조합 벡터 및 상기 벡터를 이용한 재조합 단백질의 제조 방법
BR112022010460A2 (pt) * 2019-12-02 2022-09-06 Lg Chemical Ltd Fatores secretores de proteína derivada de células de cho e vetores de expressão compreendendo os mesmos
CN112501141A (zh) * 2020-10-22 2021-03-16 重庆中元汇吉生物技术有限公司 一种增加人甲状腺过氧化物酶产量的试剂和表达方法
CN112175977B (zh) * 2020-10-30 2022-04-15 华中农业大学 一种米曲霉角蛋白酶基因及其表达载体与应用
CN112391402B (zh) * 2020-11-17 2023-03-28 华中科技大学 一种提高解脂耶氏酵母中目的蛋白表达水平的方法
CN112409464B (zh) * 2020-11-23 2022-04-22 江南大学 提高枯草芽孢杆菌重组蛋白胞外生产水平的信号肽突变体及应用
WO2023039358A1 (en) * 2021-09-09 2023-03-16 Dupont Nutrition Biosciences Aps Over expression of foldases and chaperones improves protein production
CN113736817B (zh) * 2021-10-08 2023-02-03 枣庄市杰诺生物酶有限公司 一种毕赤酵母中提高碱性脂肪酶分泌效率及酶活的方法
CN117903295B (zh) * 2024-03-19 2024-05-31 北京国科星联科技有限公司 一种用于分泌表达乳铁蛋白的马克斯克鲁维酵母及其构建方法与应用
CN117903294B (zh) * 2024-03-19 2024-06-14 北京国科星联科技有限公司 一种发酵生产乳铁蛋白的马克斯克鲁维酵母及其构建方法与应用

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Also Published As

Publication number Publication date
JP5252746B2 (ja) 2013-07-31
CO6210760A2 (es) 2010-10-20
AU2008318644A1 (en) 2009-05-07
US20090221030A1 (en) 2009-09-03
JP2011502483A (ja) 2011-01-27
CN101842479A (zh) 2010-09-22
JP2015027308A (ja) 2015-02-12
EP2203556A1 (en) 2010-07-07
MX2010004325A (es) 2010-05-20
AU2008318644B2 (en) 2013-05-02
CA2704548A1 (en) 2009-05-07
WO2009058956A1 (en) 2009-05-07
JP2013121354A (ja) 2013-06-20

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