BRPI0711811A2 - aminoácidos de cumarina fluorescente geneticamente codificados - Google Patents

aminoácidos de cumarina fluorescente geneticamente codificados Download PDF

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Publication number
BRPI0711811A2
BRPI0711811A2 BRPI0711811-2A BRPI0711811A BRPI0711811A2 BR PI0711811 A2 BRPI0711811 A2 BR PI0711811A2 BR PI0711811 A BRPI0711811 A BR PI0711811A BR PI0711811 A2 BRPI0711811 A2 BR PI0711811A2
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BR
Brazil
Prior art keywords
trna
amino acid
unnatural amino
translation system
protein
Prior art date
Application number
BRPI0711811-2A
Other languages
English (en)
Portuguese (pt)
Inventor
Jiangyun Wang
Jianming Xie
Peter G Schultz
Original Assignee
Scripps Research Inst
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Scripps Research Inst filed Critical Scripps Research Inst
Publication of BRPI0711811A2 publication Critical patent/BRPI0711811A2/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/305—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Micrococcaceae (F)
    • C07K14/31—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Micrococcaceae (F) from Staphylococcus (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
BRPI0711811-2A 2006-05-23 2007-05-21 aminoácidos de cumarina fluorescente geneticamente codificados BRPI0711811A2 (pt)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US80809906P 2006-05-23 2006-05-23
US60/808.099 2006-05-23
US81385606P 2006-06-14 2006-06-14
US60/813.856 2006-06-14
PCT/US2007/012361 WO2007139870A1 (en) 2006-05-23 2007-05-21 Genetically encoded fluorescent coumarin amino acids

Publications (1)

Publication Number Publication Date
BRPI0711811A2 true BRPI0711811A2 (pt) 2011-12-06

Family

ID=38778978

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0711811-2A BRPI0711811A2 (pt) 2006-05-23 2007-05-21 aminoácidos de cumarina fluorescente geneticamente codificados

Country Status (11)

Country Link
US (3) US7807411B2 (enExample)
EP (1) EP2018429A4 (enExample)
JP (1) JP2009538136A (enExample)
KR (1) KR20090024706A (enExample)
AU (1) AU2007267950B2 (enExample)
BR (1) BRPI0711811A2 (enExample)
CA (1) CA2652894A1 (enExample)
IL (1) IL195250A0 (enExample)
MX (1) MX2008014776A (enExample)
RU (1) RU2473690C2 (enExample)
WO (1) WO2007139870A1 (enExample)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN118359572A (zh) * 2024-04-09 2024-07-19 北京大学深圳研究生院 一种基于硫代香豆素的非天然氨基酸及其制备方法、应用

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2464532T3 (es) * 2001-04-19 2014-06-03 The Scripps Research Institute Métodos y composiciones para la producción de pares ortogonales de ARNt-aminoacil-ARNt sintetasa
EP1558747B1 (en) * 2002-10-16 2009-10-14 The Scripps Research Institute Glycoprotein synthesis
JP5642916B2 (ja) 2003-04-17 2014-12-17 ザ スクリプス リサーチ インスティテュート 真核遺伝コードの拡張
WO2005019415A2 (en) 2003-07-07 2005-03-03 The Scripps Research Institute Compositions of orthogonal lysyl-trna and aminoacyl-trna synthetase pairs and uses thereof
WO2009117726A2 (en) 2008-03-21 2009-09-24 The Regents Of The University Of California High-sensitive fluorescent energy transfer assay using fluorescent amino acids and fluoresent proteins
WO2011119771A2 (en) * 2010-03-23 2011-09-29 The Salk Institute For Biological Studies Methods and compositions for detecting protein modifications
FR2972114B1 (fr) 2011-03-01 2013-03-15 Univ Grenoble 1 Un nouveau leurre moleculaire procoagulant pour le traitement des hemophiles a ou b avec ou sans inhibiteur
CN105541962B (zh) * 2015-12-19 2021-04-02 深圳市贝沃德克生物技术研究院有限公司 基于7-羟基香豆素的定点荧光标记方法
CN105524167A (zh) * 2016-01-30 2016-04-27 深圳市贝沃德克生物技术研究院有限公司 抗乙肝e抗原的绿色荧光抗体的制备方法
CN111304234A (zh) * 2020-02-27 2020-06-19 江南大学 一种适用于枯草芽孢杆菌的非天然氨基酸利用工具

Family Cites Families (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5998593A (en) 1998-03-10 1999-12-07 Abbott Laboratories Fluorescent enzyme substrates
ES2464532T3 (es) * 2001-04-19 2014-06-03 The Scripps Research Institute Métodos y composiciones para la producción de pares ortogonales de ARNt-aminoacil-ARNt sintetasa
MXPA05003983A (es) * 2002-10-16 2005-06-22 Scripps Research Inst Incorporacion en sitio especifico de cetoaminoacidos en proteinas.
EP1558747B1 (en) * 2002-10-16 2009-10-14 The Scripps Research Institute Glycoprotein synthesis
EP1583816A4 (en) * 2002-12-22 2007-06-13 Scripps Research Inst PROTEIN ARRAYS
JP5642916B2 (ja) 2003-04-17 2014-12-17 ザ スクリプス リサーチ インスティテュート 真核遺伝コードの拡張
JP5275566B2 (ja) * 2003-06-18 2013-08-28 ザ スクリプス リサーチ インスティチュート 反応性非天然アミノ酸遺伝コード付加
WO2005019415A2 (en) * 2003-07-07 2005-03-03 The Scripps Research Institute Compositions of orthogonal lysyl-trna and aminoacyl-trna synthetase pairs and uses thereof
EP1704242A4 (en) * 2003-07-07 2008-06-18 Scripps Research Inst COMPOSITIONS WITH PAIRS OF ORTHOGONAL LEUCYL-TRNA AND AMINOACYL-TRNA-SYNTHETASE AND USES THEREOF
WO2005007624A2 (en) 2003-07-07 2005-01-27 The Scripps Research Institute Compositions of orthogonal glutamyl-trna and aminoacyl trna synthetase pairs and uses thereof
CN101532046A (zh) 2003-10-14 2009-09-16 斯克利普斯研究院 将氧化还原活性氨基酸位点特异地掺入蛋白质
CA2549830A1 (en) 2003-12-18 2006-01-05 The Scripps Research Institute Selective incorporation of 5-hyroxytryptophan into proteins in mammalian cells
CA2567624A1 (en) * 2004-05-25 2005-12-08 The Scripps Research Institute Site specific incorporation of heavy atom-containing unnatural amino acids into proteins for crystal structure determination
US20090181858A1 (en) * 2004-09-21 2009-07-16 The Scripps Research Institute Adding photoregulated amino acids to the genetic code
MX2007003142A (es) * 2004-09-21 2007-07-04 Scripps Research Inst Incorporacion in vivo de alquinil aminoacidos a proteinas en eubacterias.
EP1794312A4 (en) 2004-09-22 2009-08-12 Scripps Research Inst LOCAL LABELING OF PROTEINS FOR NMR STUDIES
WO2006045116A2 (en) * 2004-10-20 2006-04-27 The Scripps Research Institute In vivo site-specific incorporation of n-acetyl-galactosamine amino acids in eubacteria
MX2007004892A (es) 2004-10-27 2007-08-16 Scripps Research Inst Componentes de traduccion ortogonales para la incorporacion in vivo de aminoacidos no naturales.
CA2624290A1 (en) 2005-10-12 2007-04-26 Scripps Research Institute Selective posttranslational modification of phage-displayed polypeptides
MX2008011470A (es) 2006-03-09 2008-09-24 Scripps Research Inst Sistemas para la expresion de componentes de traduccion ortogonales en celulas huesped eubacterianas.
US7790847B2 (en) * 2006-03-16 2010-09-07 The Scripps Research Institute Genetically programmed expression of proteins containing the unnatural amino acid phenylselenocysteine

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN118359572A (zh) * 2024-04-09 2024-07-19 北京大学深圳研究生院 一种基于硫代香豆素的非天然氨基酸及其制备方法、应用

Also Published As

Publication number Publication date
RU2473690C2 (ru) 2013-01-27
EP2018429A1 (en) 2009-01-28
MX2008014776A (es) 2008-11-28
US20120329162A1 (en) 2012-12-27
CA2652894A1 (en) 2007-12-06
AU2007267950B2 (en) 2012-08-16
IL195250A0 (en) 2011-08-01
WO2007139870A1 (en) 2007-12-06
RU2008145487A (ru) 2010-06-27
US20080044886A1 (en) 2008-02-21
KR20090024706A (ko) 2009-03-09
US8192952B2 (en) 2012-06-05
US7807411B2 (en) 2010-10-05
JP2009538136A (ja) 2009-11-05
AU2007267950A1 (en) 2007-12-06
EP2018429A4 (en) 2010-07-14
US20090298119A1 (en) 2009-12-03

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B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B08F Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette]

Free format text: REFERENTE 6A. ANUIDADE(S).

B08K Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette]

Free format text: REFERENTE AO DESPACHO 8.6 PUBLICADO NA RPI 2226 DE 03/09/2013.