AU2007267950B2 - Genetically encoded fluorescent coumarin amino acids - Google Patents

Genetically encoded fluorescent coumarin amino acids Download PDF

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Publication number
AU2007267950B2
AU2007267950B2 AU2007267950A AU2007267950A AU2007267950B2 AU 2007267950 B2 AU2007267950 B2 AU 2007267950B2 AU 2007267950 A AU2007267950 A AU 2007267950A AU 2007267950 A AU2007267950 A AU 2007267950A AU 2007267950 B2 AU2007267950 B2 AU 2007267950B2
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AU
Australia
Prior art keywords
trna
amino acid
unnatural amino
protein
orthogonal
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Ceased
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AU2007267950A
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AU2007267950A1 (en
Inventor
Peter G. Schultz
Jiangyun Wang
Jianming Xie
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Scripps Research Institute
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Scripps Research Institute
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Ceased legal-status Critical Current
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/305—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Micrococcaceae (F)
    • C07K14/31—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Micrococcaceae (F) from Staphylococcus (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
AU2007267950A 2006-05-23 2007-05-21 Genetically encoded fluorescent coumarin amino acids Ceased AU2007267950B2 (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US80809906P 2006-05-23 2006-05-23
US60/808,099 2006-05-23
US81385606P 2006-06-14 2006-06-14
US60/813,856 2006-06-14
PCT/US2007/012361 WO2007139870A1 (en) 2006-05-23 2007-05-21 Genetically encoded fluorescent coumarin amino acids

Publications (2)

Publication Number Publication Date
AU2007267950A1 AU2007267950A1 (en) 2007-12-06
AU2007267950B2 true AU2007267950B2 (en) 2012-08-16

Family

ID=38778978

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2007267950A Ceased AU2007267950B2 (en) 2006-05-23 2007-05-21 Genetically encoded fluorescent coumarin amino acids

Country Status (11)

Country Link
US (3) US7807411B2 (enExample)
EP (1) EP2018429A4 (enExample)
JP (1) JP2009538136A (enExample)
KR (1) KR20090024706A (enExample)
AU (1) AU2007267950B2 (enExample)
BR (1) BRPI0711811A2 (enExample)
CA (1) CA2652894A1 (enExample)
IL (1) IL195250A0 (enExample)
MX (1) MX2008014776A (enExample)
RU (1) RU2473690C2 (enExample)
WO (1) WO2007139870A1 (enExample)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1490483B1 (en) * 2001-04-19 2015-06-03 The Scripps Research Institute In vivo incorporation of unnatural amino acids
KR20050073559A (ko) * 2002-10-16 2005-07-14 더 스크립스 리서치 인스티튜트 당단백질 합성
CN103289960B (zh) 2003-04-17 2017-04-26 斯克利普斯研究院 扩展真核生物遗传密码
DE602004011789T2 (de) * 2003-07-07 2009-02-12 The Scripps Research Institute, La Jolla Zusammensetzungen der orthogonalen Lysyl-tRNA und Aminoacyl-tRNA Synthetase Paaren und ihre Verwendungen
WO2009117726A2 (en) 2008-03-21 2009-09-24 The Regents Of The University Of California High-sensitive fluorescent energy transfer assay using fluorescent amino acids and fluoresent proteins
WO2011119771A2 (en) * 2010-03-23 2011-09-29 The Salk Institute For Biological Studies Methods and compositions for detecting protein modifications
FR2972114B1 (fr) 2011-03-01 2013-03-15 Univ Grenoble 1 Un nouveau leurre moleculaire procoagulant pour le traitement des hemophiles a ou b avec ou sans inhibiteur
CN105541962B (zh) * 2015-12-19 2021-04-02 深圳市贝沃德克生物技术研究院有限公司 基于7-羟基香豆素的定点荧光标记方法
CN105524167A (zh) * 2016-01-30 2016-04-27 深圳市贝沃德克生物技术研究院有限公司 抗乙肝e抗原的绿色荧光抗体的制备方法
CN111304234A (zh) * 2020-02-27 2020-06-19 江南大学 一种适用于枯草芽孢杆菌的非天然氨基酸利用工具
CN118359572B (zh) * 2024-04-09 2024-12-13 北京大学深圳研究生院 一种基于硫代香豆素的非天然氨基酸及其制备方法、应用

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002085923A2 (en) * 2001-04-19 2002-10-31 The Scripps Research Institute In vivo incorporation of unnatural amino acids

Family Cites Families (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5998593A (en) 1998-03-10 1999-12-07 Abbott Laboratories Fluorescent enzyme substrates
DK1578949T3 (da) 2002-10-16 2009-07-27 Scripps Research Inst Stedspecifik optagelse af keto-aminnosyrer hos proteiner
KR20050073559A (ko) 2002-10-16 2005-07-14 더 스크립스 리서치 인스티튜트 당단백질 합성
EP1583816A4 (en) 2002-12-22 2007-06-13 Scripps Research Inst PROTEIN ARRAYS
CN103289960B (zh) 2003-04-17 2017-04-26 斯克利普斯研究院 扩展真核生物遗传密码
WO2005003294A2 (en) * 2003-06-18 2005-01-13 The Scripps Research Institute Unnatural reactive amino acid genetic code additions
WO2005007624A2 (en) * 2003-07-07 2005-01-27 The Scripps Research Institute Compositions of orthogonal glutamyl-trna and aminoacyl trna synthetase pairs and uses thereof
WO2005007870A2 (en) * 2003-07-07 2005-01-27 The Scripps Research Institute COMPOSITIONS OF ORTHOGONAL LEUCYL-tRNA AND AMINOACYL-tRNA SYNTHETASE PAIRS AND USES THEREOF
DE602004011789T2 (de) 2003-07-07 2009-02-12 The Scripps Research Institute, La Jolla Zusammensetzungen der orthogonalen Lysyl-tRNA und Aminoacyl-tRNA Synthetase Paaren und ihre Verwendungen
WO2005038002A2 (en) * 2003-10-14 2005-04-28 The Scripps Research Institute Site-specific incorporation of redox active amino acids into proteins
BRPI0417801A (pt) * 2003-12-18 2007-04-10 Scripps Research Inst incorporação seletiva de 5-hidroxitriptofano em proteìnas de células mamìferas
AU2005248392A1 (en) 2004-05-25 2005-12-08 Irm, Llc Site specific incorporation of heavy atom-containing unnatural amino acids into proteins for crystal structure determination
EP1794323B1 (en) * 2004-09-21 2014-08-06 The Scripps Research Institute In vivo incorporation of alkynyl amino acids into proteins in eubacteria
EP1797177A4 (en) * 2004-09-21 2008-07-09 Scripps Research Inst ADDITION OF PHOTOREGULATED AMINO ACIDS TO THE GENETIC CODE
JP2008513806A (ja) * 2004-09-22 2008-05-01 ザ スクリップス リサーチ インスティテュート Ｎｍｒ試験のための蛋白質の部位特異的標識
EP1809758A4 (en) 2004-10-20 2009-04-01 Scripps Research Inst LOCAL IN VIVO INSTALLATION OF N-ACETYLGALACTOSAMINE AMINO ACIDS IN EU BACTERIA
AU2005330514B2 (en) 2004-10-27 2011-05-12 The Scripps Research Institute Orthogonal translation components for the in vivo incorporation of unnatural amino acids
CA2624290A1 (en) 2005-10-12 2007-04-26 Scripps Research Institute Selective posttranslational modification of phage-displayed polypeptides
CA2638763A1 (en) 2006-03-09 2007-09-13 The Scripps Research Institute System for the expression of orthogonal translation components in eubacterial host cells
JP5249194B2 (ja) * 2006-03-16 2013-07-31 ザ スクリプス リサーチ インスティチュート 非天然アミノ酸フェニルセレノシステインを含有するタンパク質の遺伝的にプログラムされた発現

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002085923A2 (en) * 2001-04-19 2002-10-31 The Scripps Research Institute In vivo incorporation of unnatural amino acids

Also Published As

Publication number Publication date
AU2007267950A1 (en) 2007-12-06
IL195250A0 (en) 2011-08-01
RU2473690C2 (ru) 2013-01-27
US20120329162A1 (en) 2012-12-27
RU2008145487A (ru) 2010-06-27
EP2018429A4 (en) 2010-07-14
US20080044886A1 (en) 2008-02-21
WO2007139870A1 (en) 2007-12-06
US20090298119A1 (en) 2009-12-03
MX2008014776A (es) 2008-11-28
US8192952B2 (en) 2012-06-05
EP2018429A1 (en) 2009-01-28
KR20090024706A (ko) 2009-03-09
CA2652894A1 (en) 2007-12-06
US7807411B2 (en) 2010-10-05
JP2009538136A (ja) 2009-11-05
BRPI0711811A2 (pt) 2011-12-06

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