BRPI0606657A2 - molécula de ácido nucléico isolada, construção de dna, vetor, célula vegetal, célula hospedeira, planta, semente, cassete de expressão, método para expressar uma seqüência nucleotìdica em plantas e células vegetais, método para expressar uma seqüência de nucleotìdeos em uma célula vegetal, método para expressar seletivamente uma seqüência de nucleotìdeos em raiz vegetal, ciclotìdeo isolado, método para combater uma praga de plantas - Google Patents
molécula de ácido nucléico isolada, construção de dna, vetor, célula vegetal, célula hospedeira, planta, semente, cassete de expressão, método para expressar uma seqüência nucleotìdica em plantas e células vegetais, método para expressar uma seqüência de nucleotìdeos em uma célula vegetal, método para expressar seletivamente uma seqüência de nucleotìdeos em raiz vegetal, ciclotìdeo isolado, método para combater uma praga de plantasInfo
- Publication number
- BRPI0606657A2 BRPI0606657A2 BRPI0606657-7A BRPI0606657A BRPI0606657A2 BR PI0606657 A2 BRPI0606657 A2 BR PI0606657A2 BR PI0606657 A BRPI0606657 A BR PI0606657A BR PI0606657 A2 BRPI0606657 A2 BR PI0606657A2
- Authority
- BR
- Brazil
- Prior art keywords
- plant
- nucleotide sequence
- expressing
- cell
- isolated
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 10
- 239000002773 nucleotide Substances 0.000 title abstract 7
- 125000003729 nucleotide group Chemical group 0.000 title abstract 7
- 108060002063 Cyclotide Proteins 0.000 title abstract 5
- 238000010276 construction Methods 0.000 title abstract 3
- 108020004707 nucleic acids Proteins 0.000 title abstract 3
- 150000007523 nucleic acids Chemical class 0.000 title abstract 3
- 102000039446 nucleic acids Human genes 0.000 title abstract 3
- 241000196324 Embryophyta Species 0.000 title 7
- 241000607479 Yersinia pestis Species 0.000 title 1
- 108090000623 proteins and genes Proteins 0.000 abstract 3
- 239000000203 mixture Substances 0.000 abstract 2
- 235000013311 vegetables Nutrition 0.000 abstract 2
- LUMNWCHHXDUKFI-UHFFFAOYSA-N 5-bicyclo[2.2.1]hept-2-enylmethanol Chemical compound C1C2C(CO)CC1C=C2 LUMNWCHHXDUKFI-UHFFFAOYSA-N 0.000 abstract 1
- 108020004414 DNA Proteins 0.000 abstract 1
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract 1
- 230000000692 anti-sense effect Effects 0.000 abstract 1
- 238000010348 incorporation Methods 0.000 abstract 1
- 230000001172 regenerating effect Effects 0.000 abstract 1
- 230000001105 regulatory effect Effects 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
- C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
- C12N15/8223—Vegetative tissue-specific promoters
- C12N15/8227—Root-specific
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Biomedical Technology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Gastroenterology & Hepatology (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
MOLéCULA DE áCIDO NUCLéICO ISOLADA, CONSTRUçãO DE DNA, VETOR, CéLULA VEGETAL, CéLULA HOSPEDEIRA, PLANTA, SEMENTE, CASSETE DE EXPRESSãO, MéTODO PARA EXPRESSAR UMA SEQüêNCIA NUCLEOTìDICA EM PLANTAS E CéLULAS VEGETAIS, MéTODO PARA EXPRESSAR UMA SEQüêNCIA DE NUCLEOTìDEOS EM UMA CéLULA VEGETAL, MéTODO PARA EXPRESSAR SELETIVAMENTE UMA SEQüêNCIA DE NUCLEOTìDEOS EM RAIZ VEGETAL, CICLOTìDEO ISOLADO, MéTODO PARA COMBATER UMA PRAGA DE PLANTAS. A presente invenção provê composições e métodos para regular a expressão de seqüências nucleotídicas heterólogas em uma planta. As composições incluem uma nova seqUência nucleotídica para um promotor preferencial de raiz, para o gene que codifica Cyclol. é fornecido um método para expressar uma seqUência nucleotídica heteróloga em uma planta, usando sequências promotoras aqui divulgadas. O método compreende incorporação estável, no genoma de uma célula vegetal, de uma seqüência nucleotídica operacionalemente ligada ao promotor raiz-preferencial da presente invenção; e regeneração de uma planta transformada de maneira estável que expressa a seqüência nucleotidica. A presente invenção também refere-se a ácidos nucléicos codificando ciclotídeos vegetais. A invenção refere-se à construção de um gene quimérico que codifica o todo ou uma parte de ciclotídeos vegetais, na orientação sense ou antisense, na qual a expressão do gene quimérico resulta na produção de níveis alterados de ciclotídeos vegetais em uma célula hospedeira transformada.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US64372005P | 2005-01-13 | 2005-01-13 | |
| PCT/US2006/000153 WO2006076189A2 (en) | 2005-01-13 | 2006-01-06 | Maize cyclo1 gene and promoter |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| BRPI0606657A2 true BRPI0606657A2 (pt) | 2009-07-07 |
Family
ID=36646350
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BRPI0606657-7A BRPI0606657A2 (pt) | 2005-01-13 | 2006-01-06 | molécula de ácido nucléico isolada, construção de dna, vetor, célula vegetal, célula hospedeira, planta, semente, cassete de expressão, método para expressar uma seqüência nucleotìdica em plantas e células vegetais, método para expressar uma seqüência de nucleotìdeos em uma célula vegetal, método para expressar seletivamente uma seqüência de nucleotìdeos em raiz vegetal, ciclotìdeo isolado, método para combater uma praga de plantas |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US7268226B2 (pt) |
| EP (1) | EP1846442B1 (pt) |
| AR (1) | AR051903A1 (pt) |
| AT (1) | ATE465174T1 (pt) |
| BR (1) | BRPI0606657A2 (pt) |
| CA (1) | CA2594728C (pt) |
| DE (1) | DE602006013788D1 (pt) |
| MX (1) | MX2007008486A (pt) |
| WO (1) | WO2006076189A2 (pt) |
Families Citing this family (30)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2527449A3 (en) | 2007-08-29 | 2013-04-03 | E. I. du Pont de Nemours and Company | Methods involving genes encoding nucleoside diphosphatase kinase (NDK) polypeptides and homologs thereof for modifying the plant's root architecture |
| EP2225379A2 (en) | 2007-11-20 | 2010-09-08 | E. I. du Pont de Nemours and Company | Plants with altered root architecture, related constructs and methods involving genes encoding leucine rich repeat kinase (llrk) polypeptides and homologs thereof |
| US20120023613A1 (en) | 2008-12-17 | 2012-01-26 | E.I. du Pont de Nemours and Company and Pioneer Hi-Bred International | Plants having altered agronomic characteristics under nitrogen limiting conditions and related constructs and methods involving genes encoding lnt9 polypeptides |
| US20120004114A1 (en) | 2008-12-22 | 2012-01-05 | E. I. Du Pont De Nemours And Company And Pioneer Hi-Bred International | Nucleotide sequences encoding gsh1 polypeptides and methods of use |
| WO2010104848A1 (en) | 2009-03-09 | 2010-09-16 | E. I. Du Pont De Nemours And Company | Drought tolerant plants and methods involving genes encoding type c3hc4 ring finger zinc-finger family polypeptides |
| BRPI1006249A2 (pt) | 2009-03-27 | 2019-09-24 | Du Pont | "planta, semente da planta, método para aumentar a tolerância ao estresse de nitrogênio em uma planta, método para determinar uma alteração na produção, biomassa ou ambas em uma planta e polinucleotídeo isolado" |
| EP2430162A4 (en) * | 2009-05-15 | 2013-04-24 | Ca Nat Research Council | DNA SEQUENCES AND METHOD OF PROCESSING ENCODING CARYOPHYLLACEAE AND CARYOPHYLLACEAE-SIMILAR CYCLOPEPTIDE TREATMENTS |
| US20120047603A1 (en) | 2009-06-09 | 2012-02-23 | Allen Stephen M | Drought tolerant plants and related constructs and methods involving genes encoding fatty acid desaturase family polypeptides |
| US20110035843A1 (en) | 2009-08-05 | 2011-02-10 | Pioneer Hi-Bred International, Inc. | Novel eto1 genes and use of same for reduced ethylene and improved stress tolerance in plants |
| CA2775563A1 (en) | 2009-10-15 | 2011-04-21 | E. I. Du Pont De Nemours And Company | Drought tolerant plants and related constructs and methods involving genes encoding self-incompatibility protein related polypeptides |
| AR078828A1 (es) | 2009-10-30 | 2011-12-07 | Du Pont | Plantas tolerantes a la sequia y constructos y metodos relacionados que involucran genes que codifican a los polipeptidos dtp21 |
| EP2531603A2 (en) | 2010-02-02 | 2012-12-12 | E.I. Du Pont De Nemours And Company | Plants with altered root architecture, related constructs and methods involving genes encoding lectin protein kinase (lpk) polypeptides and homologs thereof |
| US20110277183A1 (en) | 2010-04-30 | 2011-11-10 | Olga Danilevskaya | Alteration of plant architecture characteristics in plants |
| MX365030B (es) | 2010-06-25 | 2019-05-21 | Du Pont | Composiciones y metodos para mejorar la resistencia al tizon norteño de las hojas en maiz. |
| CA2814187A1 (en) | 2010-10-28 | 2012-05-03 | E. I. Du Pont De Nemours And Company | Drought tolerant plants and related constructs and methods involving genes encoding dtp6 polypeptides |
| CN103261215A (zh) | 2010-12-20 | 2013-08-21 | 纳幕尔杜邦公司 | 耐旱植物以及涉及编码mate-efflux多肽的基因的相关构建体和方法 |
| US9796764B2 (en) * | 2011-03-23 | 2017-10-24 | The University Of Queensland | Cyclotide genes in the fabaceae plant family |
| US20140298544A1 (en) | 2011-10-28 | 2014-10-02 | Pioneer Hi Bred International Inc | Engineered PEP carboxylase variants for improved plant productivity |
| WO2013109754A1 (en) | 2012-01-17 | 2013-07-25 | Pioneer Hi-Bred International, Inc. | Plant transcription factors, promoters and uses thereof |
| BR112014032080A2 (pt) | 2012-06-20 | 2017-06-27 | Cold Spring Harbor Laboratory | gene de terminação de flor (tmf) e métodos de uso. |
| BR112015018254A2 (pt) | 2013-01-31 | 2017-08-22 | Purdue Research Foundation | Métodos para produzir uma planta transgênica e para identificar um alelo, planta ou semente, semente da planta e método para identificar uma primeira planta |
| CN105247055A (zh) | 2013-03-11 | 2016-01-13 | 先锋国际良种公司 | 用于增强植物茎机械强度的组合物和方法 |
| WO2014151213A2 (en) | 2013-03-15 | 2014-09-25 | E. I. Du Pont De Nemours And Company | Drought tolerant plants and related constructs and methods involving genes encoding dtp32 polypeptides |
| CN103627707A (zh) * | 2013-10-28 | 2014-03-12 | 华大基因杨凌创新研究院有限公司 | 一种启动子OsP006、制备方法及应用 |
| CN103627708A (zh) * | 2013-10-28 | 2014-03-12 | 华大基因杨凌创新研究院有限公司 | 一种启动子OsP005、制备方法及应用 |
| CN110295246B (zh) | 2013-11-27 | 2023-06-27 | 纳幕尔杜邦公司 | 与对非生物胁迫的响应相关联的基因座 |
| BR112016015339A2 (pt) | 2013-12-30 | 2017-10-31 | E I Du Pont De Nemours And Company Us | método para aumentar pelo menos um fenótipo, planta, semente da planta, método para aumentar a tolerância ao estresse, método para selecionar tolerância ao estresse, método para selecionar uma alteração, polinucleotídeo isolado, método para produção de uma planta, método para produção de uma semente, método para produção de óleo |
| WO2016000237A1 (en) | 2014-07-03 | 2016-01-07 | Pioneer Overseas Corporation | Plants having enhanced tolerance to insect pests and related constructs and methods involving insect tolerance genes |
| CA2970138A1 (en) | 2014-12-17 | 2016-06-23 | Pioneer Hi-Bred International, Inc. | Modulation of yep6 gene expression to increase yield and other related traits in plants |
| EP4192232A2 (en) | 2020-08-10 | 2023-06-14 | E. I. du Pont de Nemours and Company | Compositions and methods for enhancing resistance to northern leaf blight in maize |
Family Cites Families (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4945050A (en) | 1984-11-13 | 1990-07-31 | Cornell Research Foundation, Inc. | Method for transporting substances into living cells and tissues and apparatus therefor |
| US4873192A (en) | 1987-02-17 | 1989-10-10 | The United States Of America As Represented By The Department Of Health And Human Services | Process for site specific mutagenesis without phenotypic selection |
| US5990387A (en) | 1988-06-10 | 1999-11-23 | Pioneer Hi-Bred International, Inc. | Stable transformation of plant cells |
| US5879918A (en) | 1989-05-12 | 1999-03-09 | Pioneer Hi-Bred International, Inc. | Pretreatment of microprojectiles prior to using in a particle gun |
| US5837848A (en) * | 1990-03-16 | 1998-11-17 | Zeneca Limited | Root-specific promoter |
| EP0452269B1 (en) | 1990-04-12 | 2002-10-09 | Syngenta Participations AG | Tissue-preferential promoters |
| US5932782A (en) | 1990-11-14 | 1999-08-03 | Pioneer Hi-Bred International, Inc. | Plant transformation method using agrobacterium species adhered to microprojectiles |
| EP0652965A1 (en) | 1992-07-27 | 1995-05-17 | Pioneer Hi-Bred International, Inc. | An improved method of agrobacterium-mediated transformation of cultured soybean cells |
| FR2712302B1 (fr) | 1993-11-10 | 1996-01-05 | Rhone Poulenc Agrochimie | Eléments promoteurs de gènes chimères de tubuline alpha. |
| US5605793A (en) | 1994-02-17 | 1997-02-25 | Affymax Technologies N.V. | Methods for in vitro recombination |
| US5837458A (en) | 1994-02-17 | 1998-11-17 | Maxygen, Inc. | Methods and compositions for cellular and metabolic engineering |
| US5837876A (en) * | 1995-07-28 | 1998-11-17 | North Carolina State University | Root cortex specific gene promoter |
| US5981840A (en) | 1997-01-24 | 1999-11-09 | Pioneer Hi-Bred International, Inc. | Methods for agrobacterium-mediated transformation |
| ATE332385T1 (de) * | 1997-03-03 | 2006-07-15 | Syngenta Participations Ag | Erfahren zur herstellung von hybridem saatgut mittels bedingter weiblicher sterilität |
| US6506559B1 (en) | 1997-12-23 | 2003-01-14 | Carnegie Institute Of Washington | Genetic inhibition by double-stranded RNA |
| US6774282B1 (en) * | 1999-03-08 | 2004-08-10 | Pioneer Hi-Bred International, Inc. | Maize metallothionein gene and promoter |
| AUPQ339899A0 (en) | 1999-10-13 | 1999-11-04 | University Of Queensland, The | Novel molecules |
| JP2003527834A (ja) | 1999-11-05 | 2003-09-24 | ザ・ユニバーシティ・オブ・クイーンズランド | 新規な核酸分子 |
| AU2002354015B2 (en) * | 2001-11-07 | 2007-04-26 | Syngenta Participations Ag | Promoters for regulation of gene expression in plant roots |
| US7205454B2 (en) | 2002-07-31 | 2007-04-17 | Bayer Bioscience N.V. | Corn root preferential promoters and uses thereof |
-
2006
- 2006-01-06 US US11/327,727 patent/US7268226B2/en active Active
- 2006-01-06 EP EP06717372A patent/EP1846442B1/en not_active Not-in-force
- 2006-01-06 MX MX2007008486A patent/MX2007008486A/es active IP Right Grant
- 2006-01-06 AT AT06717372T patent/ATE465174T1/de not_active IP Right Cessation
- 2006-01-06 BR BRPI0606657-7A patent/BRPI0606657A2/pt not_active IP Right Cessation
- 2006-01-06 CA CA2594728A patent/CA2594728C/en active Active
- 2006-01-06 DE DE602006013788T patent/DE602006013788D1/de active Active
- 2006-01-06 WO PCT/US2006/000153 patent/WO2006076189A2/en active Application Filing
- 2006-01-13 AR ARP060100149A patent/AR051903A1/es not_active Application Discontinuation
Also Published As
| Publication number | Publication date |
|---|---|
| WO2006076189A3 (en) | 2007-02-22 |
| WO2006076189A2 (en) | 2006-07-20 |
| CA2594728A1 (en) | 2006-07-20 |
| DE602006013788D1 (de) | 2010-06-02 |
| US7268226B2 (en) | 2007-09-11 |
| EP1846442A2 (en) | 2007-10-24 |
| AR051903A1 (es) | 2007-02-14 |
| EP1846442B1 (en) | 2010-04-21 |
| ATE465174T1 (de) | 2010-05-15 |
| CA2594728C (en) | 2013-03-19 |
| MX2007008486A (es) | 2008-02-15 |
| US20060156439A1 (en) | 2006-07-13 |
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