BRPI0418641A - organização similar a tecido de células e construtos similares a tecidos macroscópicos, gerados por cultura de macromassa de células, e método de cultura de macromassa - Google Patents

organização similar a tecido de células e construtos similares a tecidos macroscópicos, gerados por cultura de macromassa de células, e método de cultura de macromassa

Info

Publication number
BRPI0418641A
BRPI0418641A BRPI0418641-9A BRPI0418641A BRPI0418641A BR PI0418641 A BRPI0418641 A BR PI0418641A BR PI0418641 A BRPI0418641 A BR PI0418641A BR PI0418641 A BRPI0418641 A BR PI0418641A
Authority
BR
Brazil
Prior art keywords
tissue
cells
culture
organization
macroscopic
Prior art date
Application number
BRPI0418641-9A
Other languages
English (en)
Inventor
Sharadchandra Manish Deshpande
Vinoy Manoj Mojamdar
Original Assignee
Reliance Life Sciences Pvt Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Reliance Life Sciences Pvt Ltd filed Critical Reliance Life Sciences Pvt Ltd
Publication of BRPI0418641A publication Critical patent/BRPI0418641A/pt
Publication of BRPI0418641C1 publication Critical patent/BRPI0418641C1/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0062—General methods for three-dimensional culture
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Materials For Medical Uses (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Prostheses (AREA)

Abstract

ORGANIZAçãO SIMILAR A TECIDO DE CéLULAS E CONSTRUTOS SIMILARES A TECIDOS MACROSCóPICOS, GERADOS POR CULTURA DE MACROMASSA DE CéLULAS, E MéTODO DE CULTURA DE MACROMASSA" Trata-se de uma organização similar a tecido tridimensional de células pela cultura de elevada densidade de semeadura de células denominada como cultura de macromassa. Através da cultura de macromassa, as células podem ser levadas a se organizar em uma forma similar a tecido sem a ajuda de uma estrutura de suporte, e construtos similares a tecidos macroscópicos tridimensionais podem ser obtidos integralmente a partir das células. A organização similar a tecido e os construtos parecidos com tecidos macroscópicos podem ser gerados a partir de células fibroblásticas de origem mesenquimal (pelo menos), as quais podem ser tanto células diferenciadas quanto células tronco adultas multipotentes. Neste trabalho, a organização similar a tecido e os construtos similares a tecidos macroscópicos foram gerados a partir de fibroblastos dermais, células osteogênicas derivadas de células estromais de adipose, condrócitos, e de osteoblastos. O fator que causa a formação de tecido macroscópico é a cultura em larga escala a uma elevada densidade de semeadura de células por unidade de área ou espaço tridimensional, isto é, a cultura de macromassa obtida em uma larga escala. Nenhuma estrutura de suporte ou matriz estranha é utilizada para a geração dos tecidos, os tecidos são de origem completamente celular. Nenhum outro agente (exceto a elevad densidade de semeadura de células) que ajuda na formação dos tecidos, tais como produtos químicos de indução de tecido, fatores do crescimento de indução de tecido, substrato com propriedades especiais, cultura rotativa, etc., é empregado para a formação dos tecidos. Essas massas similares a tecidos podem ser potencialmente utilizadas como substituições de tecido no corpo humano. A organização similar a tecido pela cultura de macromassa de elevada densidade de semeadura de células também podem ser gerada ao nível microscópico.
BRC10418641-9A 2004-03-31 2004-03-31 equivalente tecidual tridimensional usando cultura de macromassa BRPI0418641C1 (pt)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/IN2004/000086 WO2005095585A1 (en) 2004-03-31 2004-03-31 Tissue-like organization of cells and macroscopic tissue-like constructs, generated by macromass culture of cells, and the method of macromass culture

Publications (2)

Publication Number Publication Date
BRPI0418641A true BRPI0418641A (pt) 2007-08-07
BRPI0418641C1 BRPI0418641C1 (pt) 2009-11-24

Family

ID=34958569

Family Applications (1)

Application Number Title Priority Date Filing Date
BRC10418641-9A BRPI0418641C1 (pt) 2004-03-31 2004-03-31 equivalente tecidual tridimensional usando cultura de macromassa

Country Status (10)

Country Link
EP (1) EP1730264B1 (pt)
CN (1) CN1938419B (pt)
AT (1) ATE447609T1 (pt)
AU (1) AU2004317828B2 (pt)
BR (1) BRPI0418641C1 (pt)
CA (1) CA2566374A1 (pt)
DE (1) DE602004023974D1 (pt)
HK (1) HK1092507A1 (pt)
IL (2) IL178265A (pt)
WO (1) WO2005095585A1 (pt)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7993922B2 (en) 2002-10-18 2011-08-09 Reliance Life Sciences Pvt. Ltd. Three-dimensional tissue equivalent using macromass culture
EP2455111B1 (en) * 2006-06-27 2017-08-16 Shiseido Company, Limited Cell mass capable of serving as a primitive organ-like structure comprised of a plurality of cell types of somatic origin
CA2678695A1 (en) 2006-12-08 2008-06-12 Reliance Life Sciences Pvt. Ltd. Three-dimensional tissue equivalent using macromass culture
EP2138571B1 (en) 2008-06-26 2017-04-12 SpheroTec GmbH Process for the preparation of multicellular spheroids
KR101733137B1 (ko) * 2015-12-30 2017-05-08 (주)엑셀세라퓨틱스 연골조직 제조를 위한 3차원 오가노이드 블록 제작 방법
CN106754669B (zh) * 2016-11-23 2020-04-17 河海大学常州校区 基于反应-扩散模型的多细胞结构的制备方法及制备系统
CN110157617B (zh) * 2019-04-29 2021-07-16 重庆大学附属肿瘤医院 宫颈癌细胞三维培养装置及其应用

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6197586B1 (en) * 1997-12-12 2001-03-06 The Regents Of The University Of California Chondrocyte-like cells useful for tissue engineering and methods
CN1180079C (zh) * 1998-11-12 2004-12-15 基质细胞有限责任公司 在三维装置中从造血祖细胞产生淋巴组织-特异细胞
US6429013B1 (en) * 1999-08-19 2002-08-06 Artecel Science, Inc. Use of adipose tissue-derived stromal cells for chondrocyte differentiation and cartilage repair

Also Published As

Publication number Publication date
WO2005095585A1 (en) 2005-10-13
IL187448A (en) 2012-05-31
CA2566374A1 (en) 2005-10-13
AU2004317828A1 (en) 2005-10-13
BRPI0418641C1 (pt) 2009-11-24
AU2004317828B2 (en) 2010-06-24
CN1938419A (zh) 2007-03-28
EP1730264B1 (en) 2009-11-04
DE602004023974D1 (de) 2009-12-17
EP1730264A1 (en) 2006-12-13
ATE447609T1 (de) 2009-11-15
CN1938419B (zh) 2011-12-14
IL178265A (en) 2010-11-30
HK1092507A1 (en) 2007-02-09
IL187448A0 (en) 2008-02-09
IL178265A0 (en) 2006-12-31

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