BRPI0418073A - oligonucleotìdeos para a detecção da leishmaniose e processos para a mesma - Google Patents
oligonucleotìdeos para a detecção da leishmaniose e processos para a mesmaInfo
- Publication number
- BRPI0418073A BRPI0418073A BRPI0418073-9A BRPI0418073A BRPI0418073A BR PI0418073 A BRPI0418073 A BR PI0418073A BR PI0418073 A BRPI0418073 A BR PI0418073A BR PI0418073 A BRPI0418073 A BR PI0418073A
- Authority
- BR
- Brazil
- Prior art keywords
- seq
- pkdl
- leishmaniasis
- amplified fragments
- detection
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/6893—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for protozoa
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/16—Primer sets for multiplex assays
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Organic Chemistry (AREA)
- Analytical Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- General Health & Medical Sciences (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Pathology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Crystals, And After-Treatments Of Crystals (AREA)
Abstract
"OLIGONUCLEOTìDEOS PARA A DETECçãO DA LEISHMANIOSE E PROCESSOS PARA A MESMA". A presente invenção refere-se a novos iniciadores de oligonucleotídeos que possuem SEQ ID N<0>: 1, SEQ ID N<0>: 2, SEQ ID N<0>: 3 e SEQ ID N<0>: 4 para a amplificação do gene relacionado com a quinesina das espécies de Leishmania. A invenção fornece ainda um processo para a detecção e para a diferenciação das cepas de Leishmania donovani causadoras da leishmaniose visceral (VL) e da leishmaniose dérmica pós kala-azar (PKDL) em uma amostra, que compreende o isolamento do DNA de uma amostra; a amplificação da região alvo partindo dos novos iniciadores de oligonucleotídeos e DNA polimerase estável ao calor para a obtenção de fragmentos amplificados; a separação dos fragmentos amplificados e análise dos fragmentos para detectar e diferenciar cepas de Leishmania donovani causadoras de VL e PKDL com base no padrão de bandas dos fragmentos amplificados. Em adição, a invenção fornece um kit para diagnóstico para a detecção e a diferenciação de cepas de Leishmania donovani causadoras de VL e PKDL.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| IN1598DE2003 | 2003-12-23 | ||
| PCT/IN2004/000395 WO2005061729A1 (en) | 2003-12-23 | 2004-12-22 | Oligonucleotides for detection of leishmaniasis and methods thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| BRPI0418073A true BRPI0418073A (pt) | 2007-04-17 |
Family
ID=34708480
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BRPI0418073-9A BRPI0418073A (pt) | 2003-12-23 | 2004-12-22 | oligonucleotìdeos para a detecção da leishmaniose e processos para a mesma |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US20070134671A1 (pt) |
| EP (1) | EP1709191B1 (pt) |
| CN (1) | CN1973049A (pt) |
| AT (1) | ATE423855T1 (pt) |
| BR (1) | BRPI0418073A (pt) |
| DE (1) | DE602004019694D1 (pt) |
| ES (1) | ES2323131T3 (pt) |
| WO (1) | WO2005061729A1 (pt) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111549160A (zh) * | 2020-05-08 | 2020-08-18 | 刘东立 | 一种用于检测利什曼原虫的引物组、试剂盒及其应用 |
| CN116411088B (zh) * | 2023-03-31 | 2024-05-24 | 黑龙江八一农垦大学 | 区分短肠重盘吸虫和日本重盘吸虫的pcr-rflp引物及方法 |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5834592A (en) * | 1995-09-22 | 1998-11-10 | Corixa Corporation | Leishmania antigens for use in the therapy and diagnosis of Leishmaniasis |
| US6855522B2 (en) * | 2002-02-27 | 2005-02-15 | Indian Council Of Medical Research | Species-specific PCR assay for detection of Leishmania donovani in clinical samples of kala-azar and post kala-azar dermal leishmaniasis |
-
2004
- 2004-12-22 DE DE602004019694T patent/DE602004019694D1/de active Active
- 2004-12-22 AT AT04806773T patent/ATE423855T1/de not_active IP Right Cessation
- 2004-12-22 EP EP04806773A patent/EP1709191B1/en not_active Not-in-force
- 2004-12-22 US US10/584,454 patent/US20070134671A1/en not_active Abandoned
- 2004-12-22 WO PCT/IN2004/000395 patent/WO2005061729A1/en active Application Filing
- 2004-12-22 CN CNA200480041524XA patent/CN1973049A/zh active Pending
- 2004-12-22 ES ES04806773T patent/ES2323131T3/es active Active
- 2004-12-22 BR BRPI0418073-9A patent/BRPI0418073A/pt not_active Application Discontinuation
Also Published As
| Publication number | Publication date |
|---|---|
| CN1973049A (zh) | 2007-05-30 |
| EP1709191A1 (en) | 2006-10-11 |
| US20070134671A1 (en) | 2007-06-14 |
| ATE423855T1 (de) | 2009-03-15 |
| DE602004019694D1 (de) | 2009-04-09 |
| WO2005061729A1 (en) | 2005-07-07 |
| ES2323131T3 (es) | 2009-07-07 |
| EP1709191B1 (en) | 2009-02-25 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| B11A | Dismissal acc. art.33 of ipl - examination not requested within 36 months of filing | ||
| B11Y | Definitive dismissal - extension of time limit for request of examination expired [chapter 11.1.1 patent gazette] |