BR9916731A - Co-expressão de proteìnas - Google Patents

Co-expressão de proteìnas

Info

Publication number
BR9916731A
BR9916731A BR9916731-0A BR9916731A BR9916731A BR 9916731 A BR9916731 A BR 9916731A BR 9916731 A BR9916731 A BR 9916731A BR 9916731 A BR9916731 A BR 9916731A
Authority
BR
Brazil
Prior art keywords
protein
plant
proteins
high levels
zein
Prior art date
Application number
BR9916731-0A
Other languages
English (en)
Inventor
Suman Bagga
Champa Sengupta-Gopalan
John D Kemp
Original Assignee
Univ New Mexico State Tech Tra
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ New Mexico State Tech Tra filed Critical Univ New Mexico State Tech Tra
Publication of BR9916731A publication Critical patent/BR9916731A/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8251—Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis
    • C12N15/8254—Tryptophan or lysine
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C07K14/425—Zeins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8251—Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8251—Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis
    • C12N15/8253—Methionine or cysteine

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Nutrition Science (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Botany (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Fodder In General (AREA)

Abstract

Patente de Invenção: "CO-EXPRESSãO DE PROTEìNAS". A presente invenção refere-se a materiais e processos para plantas e tecidos vegetais transformados que são capazes de expressar altos níveis de proteínas estáveis que estão localizadas na forma de corpos protéicos dentro de uma célula vegetal. São divulgadas plantas transformadas co-expressam altos níveis de ambas as proteínas zeínas de 15 kD e de 10 kD que acumulam altos níveis na forma de corpos protéicos no tecido vegetativo da planta. As plantas transformadas que co-expressam as proteínas zeínas de 15 kD e de 10 kD são úteis para fornecer plantações de forragem contendo níveis aumentados de aminoácidos contendo enxofre, tal como a metionina, na dieta de animais que normalmente se alimentam de tais plantações. São também consideradas pela presente invenção as plantas ou os tecidos vegetais transformados que compreendem corpos protéicos estáveis que contêm meterial proteináceo heterólogo. Em uma modalidade, um corpo protéico estável é expresso em uma planta ou tecido vegetal na forma de uma proteína de fusão que compreende uma proteína zeína e uma proteína ou peptídeo ligado de forma operacional. Os corpos protéicos fornecidos na presente invenção são resistentes à digestão no rúmen ou à degradação ambiental.
BR9916731-0A 1998-12-31 1999-04-16 Co-expressão de proteìnas BR9916731A (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US22465598A 1998-12-31 1998-12-31
PCT/US1999/008525 WO2000040738A1 (en) 1998-12-31 1999-04-16 Co-expression of proteins

Publications (1)

Publication Number Publication Date
BR9916731A true BR9916731A (pt) 2001-11-06

Family

ID=22841589

Family Applications (1)

Application Number Title Priority Date Filing Date
BR9916731-0A BR9916731A (pt) 1998-12-31 1999-04-16 Co-expressão de proteìnas

Country Status (8)

Country Link
EP (1) EP1141347A1 (pt)
JP (1) JP2002541768A (pt)
CN (1) CN1334877A (pt)
AR (1) AR016256A1 (pt)
AU (1) AU3652299A (pt)
BR (1) BR9916731A (pt)
CA (1) CA2358548A1 (pt)
WO (1) WO2000040738A1 (pt)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2224792B1 (es) * 2002-06-28 2007-02-16 Era Plantech, S.L. Produccion de peptidos y proteinas por acumulacion de cuerpos proteicos derivados de reticulos endoplasmico en plantas.
AR047658A1 (es) 2004-02-03 2006-02-01 Cargill Inc Concentrado de proteinas y corriente acuosa con carbohidratos hidrosolubles
JP5667062B2 (ja) 2008-10-10 2015-02-12 エラ、ビオテック、ソシエダッド、アノニマＥｒａ Ｂｉｏｔｅｃｈ， Ｓ．Ａ． 免疫原特異的アジュバントとしての組換えタンパク粒
EP2418284A1 (en) 2010-08-13 2012-02-15 ERA Biotech, S.A. Protein body-inducing polypeptide sequences
EP2649092A1 (en) * 2010-12-10 2013-10-16 BASF Plant Science Company GmbH Modified protein body tags and production methods thereof
JP2015131781A (ja) * 2014-01-14 2015-07-23 カタギ食品株式会社 ポリクローナル抗体、ゴマアレルゲンの検出用キット、低アレルゲンゴマの判別方法、低アレルゲンゴマの作出方法及び低アレルゲンゴマ

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2744134B1 (fr) * 1996-01-29 1998-04-03 Biocem Proteines de reserve de plantes enrichies en acides amines, notamment alpha-zeine de mais enrichie en lysine plantes exprimant ces proteines

Also Published As

Publication number Publication date
JP2002541768A (ja) 2002-12-10
AU3652299A (en) 2000-07-24
CN1334877A (zh) 2002-02-06
AR016256A1 (es) 2001-06-20
CA2358548A1 (en) 2000-07-13
WO2000040738A1 (en) 2000-07-13
EP1141347A1 (en) 2001-10-10

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