WO2002086077A3 - Co-expression of zein proteins - Google Patents
Co-expression of zein proteins Download PDFInfo
- Publication number
- WO2002086077A3 WO2002086077A3 PCT/US2002/012646 US0212646W WO02086077A3 WO 2002086077 A3 WO2002086077 A3 WO 2002086077A3 US 0212646 W US0212646 W US 0212646W WO 02086077 A3 WO02086077 A3 WO 02086077A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- plant
- protein
- high levels
- transformed plants
- zein proteins
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
- C07K14/425—Zeins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
- C12N15/8251—Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicinal Chemistry (AREA)
- Gastroenterology & Hepatology (AREA)
- Botany (AREA)
- Nutrition Science (AREA)
- Cell Biology (AREA)
- Peptides Or Proteins (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
Methods and constructs for transformed plants and plant tissues that are capable of expressing high levels of stable proteins localized as protein bodies within the plant cell. Transformed plants co-expressing high levels of both the 15 kD and 10 kD zein proteins are disclosed which accumulate to high levels as protein bodies in the vegetative tissue of the plant. Transformed plants co-expressing the 15 kD and 10 kD zein proteins are useful for providing forage crops containing increased levels of sulfur containing amino acids, such as methionine, in the diet of animals that normally feed on such crops. In one embodiment, a stable protein body is expressed in a plant or plant tissue as a fusion protein comprising both the 15 kD and 10 kD zein proteins operably linked by a polypeptide or peptide linker. The protein bodies provided in the present invention are resistant to rumin digestion or environmental degradation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| AU2002256310A AU2002256310A1 (en) | 2001-04-18 | 2002-04-18 | Co-expression of zein proteins |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US28473201P | 2001-04-18 | 2001-04-18 | |
| US60/284,732 | 2001-04-18 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| WO2002086077A2 WO2002086077A2 (en) | 2002-10-31 |
| WO2002086077A3 true WO2002086077A3 (en) | 2004-10-21 |
Family
ID=23091316
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/US2002/012646 WO2002086077A2 (en) | 2001-04-18 | 2002-04-18 | Co-expression of zein proteins |
Country Status (2)
| Country | Link |
|---|---|
| AU (1) | AU2002256310A1 (en) |
| WO (1) | WO2002086077A2 (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108403662B (en) * | 2018-05-08 | 2020-05-22 | 华南理工大学 | Trihydroxymethyl aminomethane modified zein functional drug-loaded microsphere and preparation method thereof |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES2224792B1 (en) | 2002-06-28 | 2007-02-16 | Era Plantech, S.L. | PRODUCTION OF PEPTIDES AND PROTEINS BY ACCUMULATION OF PROTEIN BODIES DERIVED FROM ENDOPLASMIC RETICLES IN PLANTS. |
| WO2005086813A2 (en) * | 2004-03-08 | 2005-09-22 | Syngenta Participations Ag | Glutamine-rich maize seed protein and promoter |
| GB0426160D0 (en) | 2004-11-29 | 2004-12-29 | Era Plantech S L | Production of proteins |
| US8163880B2 (en) | 2006-02-23 | 2012-04-24 | Era Biotech S.A. | Production of biologically active proteins |
| KR20110084906A (en) | 2008-10-10 | 2011-07-26 | 에라 바이오테크, 에스.에이. | Recombinant protein bodies as immunogen-specific adjuvants |
| EP2418284A1 (en) | 2010-08-13 | 2012-02-15 | ERA Biotech, S.A. | Protein body-inducing polypeptide sequences |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5990384A (en) * | 1996-05-31 | 1999-11-23 | New Mexico State University | Co-expression of proteins |
-
2002
- 2002-04-18 AU AU2002256310A patent/AU2002256310A1/en not_active Abandoned
- 2002-04-18 WO PCT/US2002/012646 patent/WO2002086077A2/en not_active Application Discontinuation
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5990384A (en) * | 1996-05-31 | 1999-11-23 | New Mexico State University | Co-expression of proteins |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108403662B (en) * | 2018-05-08 | 2020-05-22 | 华南理工大学 | Trihydroxymethyl aminomethane modified zein functional drug-loaded microsphere and preparation method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| WO2002086077A2 (en) | 2002-10-31 |
| AU2002256310A1 (en) | 2002-11-05 |
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