BR9406564A - Processo para a separação de uma enzima de uma solução aquosa - Google Patents

Processo para a separação de uma enzima de uma solução aquosa

Info

Publication number
BR9406564A
BR9406564A BR9406564-0A BR9406564A BR9406564A BR 9406564 A BR9406564 A BR 9406564A BR 9406564 A BR9406564 A BR 9406564A BR 9406564 A BR9406564 A BR 9406564A
Authority
BR
Brazil
Prior art keywords
enzyme
pct
aqueous solution
separation
sec
Prior art date
Application number
BR9406564-0A
Other languages
English (en)
Inventor
Birgitte Mahler Nilsson
Mads Aage Laustsen
Christine Pahle
Original Assignee
Novo Nordisk As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=8093052&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=BR9406564(A) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Novo Nordisk As filed Critical Novo Nordisk As
Publication of BR9406564A publication Critical patent/BR9406564A/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/02—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length in solution
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/814—Enzyme separation or purification
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/814—Enzyme separation or purification
    • Y10S435/816—Enzyme separation or purification by solubility

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Separation Using Semi-Permeable Membranes (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Patente de Invenção "PROCESSO PARA A SEPARAçãO DE UMA ENZIMA DE UMA SOLUçãO AQUOSA". A presente invenção se refere a um processo de separação de uma enzima de uma solução aquosa compreendendo esta enzima na mistura com outras proteínas, e à recuperação da enzima desejada na forma cristalina.
BR9406564-0A 1993-04-02 1994-03-29 Processo para a separação de uma enzima de uma solução aquosa BR9406564A (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DK93396A DK39693D0 (da) 1993-04-02 1993-04-02 Enzym
PCT/DK1994/000132 WO1994022903A1 (en) 1993-04-02 1994-03-29 Separation of proteins

Publications (1)

Publication Number Publication Date
BR9406564A true BR9406564A (pt) 1999-08-31

Family

ID=8093052

Family Applications (1)

Application Number Title Priority Date Filing Date
BR9406564-0A BR9406564A (pt) 1993-04-02 1994-03-29 Processo para a separação de uma enzima de uma solução aquosa

Country Status (13)

Country Link
US (1) US5719048A (pt)
EP (1) EP0691982B2 (pt)
JP (1) JP3542601B2 (pt)
KR (1) KR960701082A (pt)
CN (1) CN1039244C (pt)
AT (1) ATE171189T1 (pt)
AU (1) AU6424294A (pt)
BR (1) BR9406564A (pt)
DE (1) DE69413389T2 (pt)
DK (2) DK39693D0 (pt)
ES (1) ES2124400T3 (pt)
FI (1) FI954648A (pt)
WO (1) WO1994022903A1 (pt)

Families Citing this family (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE69734708T2 (de) * 1996-03-08 2006-07-27 Novozymes A/S Kristallisation eines proteins mit einem schwefelsalz
US6031082A (en) * 1996-03-14 2000-02-29 Novo Nordisk A/S Increased yields of a crystallized protein by using a solid adsorption material
US5914141A (en) * 1997-03-11 1999-06-22 Alfacel S.A. Easy peeling wiener casings via use of enzymes
US6140475A (en) * 1997-04-11 2000-10-31 Altus Biologics Inc. Controlled dissolution crosslinked protein crystals
US20010046493A1 (en) * 2000-02-24 2001-11-29 Alex Margolin Lipase-containing composition and methods of use thereof
US7142464B2 (en) * 2003-04-29 2006-11-28 Saifun Semiconductors Ltd. Apparatus and methods for multi-level sensing in a memory array
AU2005227090B2 (en) 2004-03-22 2010-12-09 Abbott Laboratories Gmbh Oral pharmaceutical compositions of lipase-containing products, in particular of pancreatin, containing surfactants
US7823635B2 (en) * 2004-08-23 2010-11-02 Halliburton Energy Services, Inc. Downhole oil and water separator and method
RU2385736C2 (ru) * 2004-10-14 2010-04-10 Элтус Фармасьютикалз Инк. Композиции, содержащие липазу, протеазу и амилазу, предназначенные для лечения недостаточности поджелудочной железы
KR101555058B1 (ko) 2005-07-29 2015-09-22 애보트 래버러토리즈 게엠베하 살균된 판크레아틴 분말의 제조방법
US11266607B2 (en) 2005-08-15 2022-03-08 AbbVie Pharmaceuticals GmbH Process for the manufacture and use of pancreatin micropellet cores
US9198871B2 (en) 2005-08-15 2015-12-01 Abbott Products Gmbh Delayed release pancreatin compositions
US20100196344A1 (en) * 2005-10-14 2010-08-05 Cystic Fibrosis Foundation Therapeutics, Inc. Compositions and methods for treating pancreatic insufficiency
US10072256B2 (en) 2006-05-22 2018-09-11 Abbott Products Gmbh Process for separating and determining the viral load in a pancreatin sample
BRPI0721103A2 (pt) 2006-12-21 2014-03-04 Novozymes As Lipase, método de determinação do desempenho da digestão in vitro de uma lipase em comparação com uma lipase de referência, uso de uma lipase ou de uma mistura de lipases, composição farmacêutica, e, método para o tratamento de doença
WO2011000924A1 (en) 2009-07-03 2011-01-06 Abbott Products Gmbh Spray-dried amylase, pharmaceutical preparations comprising the same and use
WO2014053359A1 (en) * 2012-10-05 2014-04-10 Arcelik Anonim Sirketi Refrigerator comprising water dispenser
WO2015181118A1 (en) 2014-05-27 2015-12-03 Novozymes A/S Methods for producing lipases
WO2015181119A2 (en) 2014-05-27 2015-12-03 Novozymes A/S Lipase variants and polynucleotides encoding same
MX2017007103A (es) 2014-12-05 2017-08-24 Novozymes As Variantes de lipasa y polinucleotidos que las codifican.
EP3929285A3 (en) 2015-07-01 2022-05-25 Novozymes A/S Methods of reducing odor
EP3320089B1 (en) 2015-07-06 2021-06-16 Novozymes A/S Lipase variants and polynucleotides encoding same
US10870838B2 (en) 2015-12-01 2020-12-22 Novozymes A/S Methods for producing lipases
CN109790525A (zh) 2016-07-18 2019-05-21 诺维信公司 脂肪酶变体、编码其的多核苷酸及其用途
CA3047668A1 (en) * 2016-12-23 2018-06-28 Arla Foods Amba Production of novel beta-lactoglobulin preparations and related methods, uses, and food products
CN110651038A (zh) 2017-05-05 2020-01-03 诺维信公司 包含脂肪酶和亚硫酸盐的组合物
CN111108183A (zh) 2017-06-30 2020-05-05 诺维信公司 酶浆液组合物
EP3720954A1 (en) 2017-12-04 2020-10-14 Novozymes A/S Lipase variants and polynucleotides encoding same
WO2024032881A1 (en) 2022-08-10 2024-02-15 Arla Foods Amba Crystallisation of a protein capable of crystallizing under salting-in conditions using multiple protein feeds

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3642582A (en) * 1969-07-14 1972-02-15 Standard Brands Inc Purification of alpha amylase
US4659667A (en) * 1985-02-26 1987-04-21 Miles Laboratories, Inc. Process to recover crystalline enzymes and crystalline enzymes produced thereby
DK122686D0 (da) † 1986-03-17 1986-03-17 Novo Industri As Fremstilling af proteiner
EP0305216B1 (en) † 1987-08-28 1995-08-02 Novo Nordisk A/S Recombinant Humicola lipase and process for the production of recombinant humicola lipases

Also Published As

Publication number Publication date
WO1994022903A1 (en) 1994-10-13
EP0691982A1 (en) 1996-01-17
DE69413389T2 (de) 1999-04-29
JPH08508168A (ja) 1996-09-03
US5719048A (en) 1998-02-17
KR960701082A (ko) 1996-02-24
ATE171189T1 (de) 1998-10-15
EP0691982B2 (en) 2008-03-12
EP0691982B1 (en) 1998-09-16
DK0691982T3 (da) 1999-06-14
AU6424294A (en) 1994-10-24
CN1120340A (zh) 1996-04-10
FI954648A0 (fi) 1995-09-29
CN1039244C (zh) 1998-07-22
DK39693D0 (da) 1993-04-02
FI954648A (fi) 1995-09-29
JP3542601B2 (ja) 2004-07-14
ES2124400T3 (es) 1999-02-01
DE69413389D1 (de) 1998-10-22

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Legal Events

Date Code Title Description
EG Technical examination (opinion): publication of technical examination (opinion)
FC Decision: refusal