BR112023025269A2 - Sistemas e métodos para sequenciamento de nucleotídeos com o uso de dois canais ópticos - Google Patents
Sistemas e métodos para sequenciamento de nucleotídeos com o uso de dois canais ópticosInfo
- Publication number
- BR112023025269A2 BR112023025269A2 BR112023025269A BR112023025269A BR112023025269A2 BR 112023025269 A2 BR112023025269 A2 BR 112023025269A2 BR 112023025269 A BR112023025269 A BR 112023025269A BR 112023025269 A BR112023025269 A BR 112023025269A BR 112023025269 A2 BR112023025269 A2 BR 112023025269A2
- Authority
- BR
- Brazil
- Prior art keywords
- detector
- nucleotide
- systems
- methods
- coupled
- Prior art date
Links
- 239000002773 nucleotide Substances 0.000 title abstract 7
- 238000000034 method Methods 0.000 title abstract 3
- 230000003287 optical effect Effects 0.000 title abstract 3
- 238000012163 sequencing technique Methods 0.000 title abstract 3
- 125000003729 nucleotide group Chemical group 0.000 abstract 6
- 239000007850 fluorescent dye Substances 0.000 abstract 4
- 150000007523 nucleic acids Chemical class 0.000 abstract 2
- 238000001712 DNA sequencing Methods 0.000 abstract 1
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract 1
- 230000005284 excitation Effects 0.000 abstract 1
- 108020004707 nucleic acids Proteins 0.000 abstract 1
- 102000039446 nucleic acids Human genes 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6825—Nucleic acid detection involving sensors
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
- C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/6456—Spatial resolved fluorescence measurements; Imaging
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
- C12Q2563/107—Nucleic acid detection characterized by the use of physical, structural and functional properties fluorescence
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
- C12Q2565/60—Detection means characterised by use of a special device
- C12Q2565/607—Detection means characterised by use of a special device being a sensor, e.g. electrode
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N2021/6417—Spectrofluorimetric devices
- G01N2021/6421—Measuring at two or more wavelengths
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
- G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
- G01N2021/6441—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks with two or more labels
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Organic Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- General Physics & Mathematics (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Pathology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Optics & Photonics (AREA)
- Biomedical Technology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US17/338,590 US20220403450A1 (en) | 2021-06-03 | 2021-06-03 | Systems and methods for sequencing nucleotides using two optical channels |
| PCT/US2022/031152 WO2022256229A1 (en) | 2021-06-03 | 2022-05-26 | Systems and methods for sequencing nucleotides using two optical channels |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| BR112023025269A2 true BR112023025269A2 (pt) | 2024-02-20 |
Family
ID=82100623
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BR112023025269A BR112023025269A2 (pt) | 2021-06-03 | 2022-05-26 | Sistemas e métodos para sequenciamento de nucleotídeos com o uso de dois canais ópticos |
Country Status (11)
| Country | Link |
|---|---|
| US (1) | US20220403450A1 (enExample) |
| EP (1) | EP4347885A1 (enExample) |
| JP (1) | JP2024522090A (enExample) |
| KR (1) | KR20240017854A (enExample) |
| CN (1) | CN117460842A (enExample) |
| AU (1) | AU2022286318A1 (enExample) |
| BR (1) | BR112023025269A2 (enExample) |
| CA (1) | CA3217004A1 (enExample) |
| IL (1) | IL307901A (enExample) |
| MX (1) | MX2023014280A (enExample) |
| WO (1) | WO2022256229A1 (enExample) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2024516191A (ja) | 2021-05-05 | 2024-04-12 | イルミナ ケンブリッジ リミテッド | ビス－ホウ素縮合複素環を含有する蛍光色素及び配列決定における使用 |
| AU2023246772A1 (en) | 2022-03-29 | 2024-01-18 | Illumina Inc. | Chromenoquinoline dyes and uses in sequencing |
| CN119604513A (zh) | 2022-06-28 | 2025-03-11 | 因美纳公司 | 含有稠合四环双硼杂环的荧光染料及其在测序中的用途 |
| WO2024206407A2 (en) | 2023-03-29 | 2024-10-03 | Illumina, Inc. | Naphthalimide dyes and uses in nucleic acid sequencing |
| WO2025090596A1 (en) | 2023-10-26 | 2025-05-01 | Illumina, Inc. | 4,5-substituted naphthalimide dyes and uses in nucleic acid sequencing |
| US20250163505A1 (en) | 2023-11-17 | 2025-05-22 | Illumina, Inc. | Conjugated polymers or polymer dots as fluorescent labels |
| US20250197933A1 (en) | 2023-12-18 | 2025-06-19 | Illumina, Inc. | Hydrogel nanoparticles as labeling scaffold in sequencing |
| US20250215494A1 (en) | 2023-12-29 | 2025-07-03 | Illumina, Inc. | Tricyclic polymethine dyes for nucleic acid sequencing |
| WO2025160569A1 (en) * | 2024-01-26 | 2025-07-31 | Pumpkinseed Technologies, Inc. | Methods and systems for nucleic acid sequence determination |
Family Cites Families (34)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0874B2 (ja) | 1990-07-27 | 1996-01-10 | アイシス・ファーマシューティカルス・インコーポレーテッド | 遺伝子発現を検出および変調するヌクレアーゼ耐性、ピリミジン修飾オリゴヌクレオチド |
| US5432272A (en) | 1990-10-09 | 1995-07-11 | Benner; Steven A. | Method for incorporating into a DNA or RNA oligonucleotide using nucleotides bearing heterocyclic bases |
| AU3222793A (en) | 1991-11-26 | 1993-06-28 | Gilead Sciences, Inc. | Enhanced triple-helix and double-helix formation with oligomers containing modified pyrimidines |
| DK0691980T3 (da) | 1993-03-30 | 1997-12-29 | Sanofi Sa | 7-deazapurinmodifiderende oligonukleotider |
| EP0695306A1 (en) | 1993-04-19 | 1996-02-07 | Gilead Sciences, Inc. | Enhanced triple-helix and double-helix formation with oligomers containing modified purines |
| US5846719A (en) | 1994-10-13 | 1998-12-08 | Lynx Therapeutics, Inc. | Oligonucleotide tags for sorting and identification |
| US6150510A (en) | 1995-11-06 | 2000-11-21 | Aventis Pharma Deutschland Gmbh | Modified oligonucleotides, their preparation and their use |
| US5750341A (en) | 1995-04-17 | 1998-05-12 | Lynx Therapeutics, Inc. | DNA sequencing by parallel oligonucleotide extensions |
| WO1998023733A2 (en) | 1996-11-27 | 1998-06-04 | University Of Washington | Thermostable polymerases having altered fidelity |
| JP2002503954A (ja) | 1997-04-01 | 2002-02-05 | グラクソ、グループ、リミテッド | 核酸増幅法 |
| US6969488B2 (en) | 1998-05-22 | 2005-11-29 | Solexa, Inc. | System and apparatus for sequential processing of analytes |
| US6329178B1 (en) | 2000-01-14 | 2001-12-11 | University Of Washington | DNA polymerase mutant having one or more mutations in the active site |
| US7057026B2 (en) | 2001-12-04 | 2006-06-06 | Solexa Limited | Labelled nucleotides |
| WO2004018497A2 (en) | 2002-08-23 | 2004-03-04 | Solexa Limited | Modified nucleotides for polynucleotide sequencing |
| GB0321306D0 (en) | 2003-09-11 | 2003-10-15 | Solexa Ltd | Modified polymerases for improved incorporation of nucleotide analogues |
| EP1701785A1 (en) | 2004-01-07 | 2006-09-20 | Solexa Ltd. | Modified molecular arrays |
| US20070048748A1 (en) | 2004-09-24 | 2007-03-01 | Li-Cor, Inc. | Mutant polymerases for sequencing and genotyping |
| WO2006064199A1 (en) | 2004-12-13 | 2006-06-22 | Solexa Limited | Improved method of nucleotide detection |
| EP1888743B1 (en) | 2005-05-10 | 2011-08-03 | Illumina Cambridge Limited | Improved polymerases |
| CA2615323A1 (en) | 2005-06-06 | 2007-12-21 | 454 Life Sciences Corporation | Paired end sequencing |
| GB0514936D0 (en) | 2005-07-20 | 2005-08-24 | Solexa Ltd | Preparation of templates for nucleic acid sequencing |
| US7329860B2 (en) | 2005-11-23 | 2008-02-12 | Illumina, Inc. | Confocal imaging methods and apparatus |
| WO2009097368A2 (en) * | 2008-01-28 | 2009-08-06 | Complete Genomics, Inc. | Methods and compositions for efficient base calling in sequencing reactions |
| US8965076B2 (en) | 2010-01-13 | 2015-02-24 | Illumina, Inc. | Data processing system and methods |
| US9029103B2 (en) | 2010-08-27 | 2015-05-12 | Illumina Cambridge Limited | Methods for sequencing polynucleotides |
| US8951781B2 (en) | 2011-01-10 | 2015-02-10 | Illumina, Inc. | Systems, methods, and apparatuses to image a sample for biological or chemical analysis |
| CN204832037U (zh) | 2012-04-03 | 2015-12-02 | 伊鲁米那股份有限公司 | 检测设备 |
| DE102014006003A1 (de) | 2014-04-28 | 2015-10-29 | Merck Patent Gmbh | Leuchtstoffe |
| GB201508858D0 (en) * | 2015-05-22 | 2015-07-01 | Illumina Cambridge Ltd | Polymethine compounds with long stokes shifts and their use as fluorescent labels |
| GB201516987D0 (en) | 2015-09-25 | 2015-11-11 | Illumina Cambridge Ltd | Polymethine compounds and their use as fluorescent labels |
| US10385214B2 (en) | 2016-09-30 | 2019-08-20 | Illumina Cambridge Limited | Fluorescent dyes and their uses as biomarkers |
| BR112019014683A2 (pt) * | 2017-03-07 | 2020-02-18 | Illumina, Inc. | Sequenciamento com dois canais óticos, com fonte de luz única |
| CA3103636A1 (en) | 2019-03-01 | 2020-09-10 | Illumina Cambridge Limited | Tertiary amine substituted coumarin compounds and their uses as fluorescent labels |
| WO2020178231A1 (en) * | 2019-03-01 | 2020-09-10 | Illumina, Inc. | Multiplexed fluorescent detection of analytes |
-
2021
- 2021-06-03 US US17/338,590 patent/US20220403450A1/en active Pending
-
2022
- 2022-05-26 BR BR112023025269A patent/BR112023025269A2/pt unknown
- 2022-05-26 IL IL307901A patent/IL307901A/en unknown
- 2022-05-26 JP JP2023572625A patent/JP2024522090A/ja active Pending
- 2022-05-26 KR KR1020237044429A patent/KR20240017854A/ko active Pending
- 2022-05-26 CN CN202280036586.XA patent/CN117460842A/zh active Pending
- 2022-05-26 CA CA3217004A patent/CA3217004A1/en active Pending
- 2022-05-26 MX MX2023014280A patent/MX2023014280A/es unknown
- 2022-05-26 AU AU2022286318A patent/AU2022286318A1/en active Pending
- 2022-05-26 EP EP22731433.3A patent/EP4347885A1/en active Pending
- 2022-05-26 WO PCT/US2022/031152 patent/WO2022256229A1/en not_active Ceased
Also Published As
| Publication number | Publication date |
|---|---|
| KR20240017854A (ko) | 2024-02-08 |
| US20220403450A1 (en) | 2022-12-22 |
| WO2022256229A9 (en) | 2023-12-21 |
| MX2023014280A (es) | 2024-01-17 |
| CN117460842A (zh) | 2024-01-26 |
| JP2024522090A (ja) | 2024-06-11 |
| AU2022286318A1 (en) | 2023-11-09 |
| IL307901A (en) | 2023-12-01 |
| CA3217004A1 (en) | 2022-12-08 |
| EP4347885A1 (en) | 2024-04-10 |
| WO2022256229A1 (en) | 2022-12-08 |
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