BR112023022509A2 - Célula de fluxo e métodos - Google Patents

Célula de fluxo e métodos

Info

Publication number
BR112023022509A2
BR112023022509A2 BR112023022509A BR112023022509A BR112023022509A2 BR 112023022509 A2 BR112023022509 A2 BR 112023022509A2 BR 112023022509 A BR112023022509 A BR 112023022509A BR 112023022509 A BR112023022509 A BR 112023022509A BR 112023022509 A2 BR112023022509 A2 BR 112023022509A2
Authority
BR
Brazil
Prior art keywords
functional group
flow cell
polymer
methods
primers
Prior art date
Application number
BR112023022509A
Other languages
English (en)
Inventor
A Brown Andrew
N George Wayne
Original Assignee
Illumina Cambridge Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Illumina Cambridge Ltd filed Critical Illumina Cambridge Ltd
Publication of BR112023022509A2 publication Critical patent/BR112023022509A2/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08F—MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVING CARBON-TO-CARBON UNSATURATED BONDS
    • C08F20/00—Homopolymers and copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and only one being terminated by only one carboxyl radical or a salt, anhydride, ester, amide, imide or nitrile thereof
    • C08F20/02—Monocarboxylic acids having less than ten carbon atoms, Derivatives thereof
    • C08F20/52—Amides or imides
    • C08F20/54—Amides, e.g. N,N-dimethylacrylamide or N-isopropylacrylamide
    • C08F20/56—Acrylamide; Methacrylamide
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/159—Microreactors, e.g. emulsion PCR or sequencing, droplet PCR, microcapsules, i.e. non-liquid containers with a range of different permeability's for different reaction components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/50—Detection characterised by immobilisation to a surface
    • C12Q2565/518—Detection characterised by immobilisation to a surface characterised by the immobilisation of the nucleic acid sample or target
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/50—Detection characterised by immobilisation to a surface
    • C12Q2565/537—Detection characterised by immobilisation to a surface characterised by the capture oligonucleotide acting as a primer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/50—Detection characterised by immobilisation to a surface
    • C12Q2565/543—Detection characterised by immobilisation to a surface characterised by the use of two or more capture oligonucleotide primers in concert, e.g. bridge amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/60—Detection means characterised by use of a special device
    • C12Q2565/626—Detection means characterised by use of a special device being a flow cytometer

Abstract

célula de fluxo e métodos. um exemplo de uma célula de fluxo inclui um substrato e um padrão de dois silanos diferentes sobre pelo menos uma porção de uma superfície do substrato. um primeiro polímero é fixado em um primeiro dos dois silanos diferentes e um segundo polímero é fixado em um segundo dos dois silanos diferentes. o primeiro e o segundo polímeros incluem, respectivamente, um primeiro grupo funcional e um segundo grupo funcional de um par de grupos funcionais, o par de grupos funcionais sendo selecionado do grupo que consiste em um grupo funcional éster ativado e um grupo funcional azida, um grupo funcional tetrazina e um grupo funcional éster ativado, e um grupo funcional tetrazina e um grupo funcional azida. um primeiro conjunto de primers é enxertado no primeiro polímero e um segundo conjunto de primers é enxertado no segundo polímero. o primeiro e o segundo conjuntos de primers são diferentes.
BR112023022509A 2021-04-30 2022-04-26 Célula de fluxo e métodos BR112023022509A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US202163182370P 2021-04-30 2021-04-30
PCT/EP2022/060972 WO2022229137A1 (en) 2021-04-30 2022-04-26 Flow cell and methods

Publications (1)

Publication Number Publication Date
BR112023022509A2 true BR112023022509A2 (pt) 2024-01-02

Family

ID=81850083

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112023022509A BR112023022509A2 (pt) 2021-04-30 2022-04-26 Célula de fluxo e métodos

Country Status (8)

Country Link
US (1) US20220372182A1 (pt)
EP (1) EP4330428A1 (pt)
KR (1) KR20240004418A (pt)
CN (1) CN117222752A (pt)
AU (1) AU2022264813A1 (pt)
BR (1) BR112023022509A2 (pt)
CA (1) CA3214697A1 (pt)
WO (1) WO2022229137A1 (pt)

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6258454B1 (en) * 1998-09-01 2001-07-10 Agilent Technologies Inc. Functionalization of substrate surfaces with silane mixtures
US9012022B2 (en) * 2012-06-08 2015-04-21 Illumina, Inc. Polymer coatings
CN109734946B (zh) * 2013-07-01 2021-10-26 Illumina公司 无催化剂的表面官能化和聚合物接枝
US10619204B2 (en) * 2014-11-11 2020-04-14 Illumina Cambridge Limited Methods and arrays for producing and sequencing monoclonal clusters of nucleic acid
AU2017382202B2 (en) * 2016-12-22 2022-06-09 Illumina Cambridge Limited Arrays including a resin film and a patterned polymer layer
KR102656824B1 (ko) * 2016-12-22 2024-04-11 일루미나, 인코포레이티드 유동셀 패키지 및 이의 제조 방법
BR112019028058A2 (pt) * 2018-06-29 2020-07-14 Illumina, Inc. células de fluxo

Also Published As

Publication number Publication date
AU2022264813A1 (en) 2023-10-19
CN117222752A (zh) 2023-12-12
KR20240004418A (ko) 2024-01-11
WO2022229137A1 (en) 2022-11-03
CA3214697A1 (en) 2022-11-03
EP4330428A1 (en) 2024-03-06
US20220372182A1 (en) 2022-11-24

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