BR112023016896A2 - Métodos para detecção de dna livre de células derivadas de doador em receptores de transplantes de múltiplos órgãos - Google Patents
Métodos para detecção de dna livre de células derivadas de doador em receptores de transplantes de múltiplos órgãosInfo
- Publication number
- BR112023016896A2 BR112023016896A2 BR112023016896A BR112023016896A BR112023016896A2 BR 112023016896 A2 BR112023016896 A2 BR 112023016896A2 BR 112023016896 A BR112023016896 A BR 112023016896A BR 112023016896 A BR112023016896 A BR 112023016896A BR 112023016896 A2 BR112023016896 A2 BR 112023016896A2
- Authority
- BR
- Brazil
- Prior art keywords
- free dna
- cell
- donor
- sequencing
- derived cell
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 3
- 238000001514 detection method Methods 0.000 title 1
- 238000012163 sequencing technique Methods 0.000 abstract 4
- 210000000056 organ Anatomy 0.000 abstract 3
- 230000003321 amplification Effects 0.000 abstract 2
- 238000003199 nucleic acid amplification method Methods 0.000 abstract 2
- 230000004544 DNA amplification Effects 0.000 abstract 1
- 206010052779 Transplant rejections Diseases 0.000 abstract 1
- 208000027418 Wounds and injury Diseases 0.000 abstract 1
- 239000008280 blood Substances 0.000 abstract 1
- 210000004369 blood Anatomy 0.000 abstract 1
- 230000006378 damage Effects 0.000 abstract 1
- 238000012165 high-throughput sequencing Methods 0.000 abstract 1
- 208000014674 injury Diseases 0.000 abstract 1
- 210000002381 plasma Anatomy 0.000 abstract 1
- 210000002966 serum Anatomy 0.000 abstract 1
- 238000002054 transplantation Methods 0.000 abstract 1
- 210000002700 urine Anatomy 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6851—Quantitative amplification
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/156—Polymorphic or mutational markers
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Analytical Chemistry (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Pathology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
métodos para detecção de dna livre de células derivadas de doador em receptores de transplantes de múltiplos órgãos. a presente divulgação fornece métodos de amplificação e sequenciamento de dna, compreendendo: extrair dna livre de células de uma amostra de sangue, plasma, soro ou urina de um receptor de transplante que recebeu transplante de um ou mais órgãos, incluindo transplante simultâneo ou sequencial de múltiplos órgãos, em que o dna livre de células compreende dna livre de células derivado do doador e dna livre de células derivado do receptor; realizar amplificação direcionada em 200 a 50.000 loci-alvo em um único volume de reação usando 200 a 50.000 pares de iniciadores, em que os loci-alvo compreendem loci polimórficos e loci não polimórficos; sequenciar os produtos de amplificação por sequenciamento de alto rendimento para obter uma leitura de sequenciamento e quantificar a quantidade de dna livre de células derivado do doador e a quantidade de dna total livre de células com base nas leituras de sequenciamento; e determinar se a quantidade de dna livre de células derivado de doador ou uma função do mesmo excede um limite de corte indicando rejeição de transplante ou lesão de enxerto.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US202163153800P | 2021-02-25 | 2021-02-25 | |
| PCT/US2022/017707 WO2022182878A1 (en) | 2021-02-25 | 2022-02-24 | Methods for detection of donor-derived cell-free dna in transplant recipients of multiple organs |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| BR112023016896A2 true BR112023016896A2 (pt) | 2023-11-21 |
Family
ID=80735621
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BR112023016896A BR112023016896A2 (pt) | 2021-02-25 | 2022-02-24 | Métodos para detecção de dna livre de células derivadas de doador em receptores de transplantes de múltiplos órgãos |
Country Status (7)
| Country | Link |
|---|---|
| EP (1) | EP4298248A1 (pt) |
| JP (1) | JP2024507536A (pt) |
| CN (1) | CN116964223A (pt) |
| AU (1) | AU2022226186A1 (pt) |
| BR (1) | BR112023016896A2 (pt) |
| CA (1) | CA3211540A1 (pt) |
| WO (1) | WO2022182878A1 (pt) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US11939634B2 (en) | 2010-05-18 | 2024-03-26 | Natera, Inc. | Methods for simultaneous amplification of target loci |
| EP3294906A1 (en) | 2015-05-11 | 2018-03-21 | Natera, Inc. | Methods and compositions for determining ploidy |
| WO2024059514A1 (en) * | 2022-09-12 | 2024-03-21 | Transplant Genomics, Inc. | Methods, systems, and compositions for diagnosing pancreatic transplant rejection |
| WO2024076484A1 (en) * | 2022-10-06 | 2024-04-11 | Natera, Inc. | Methods for determination and monitoring of xenotransplant rejection by measuring nucleic acids or proteins derived from the xenotransplant |
Family Cites Families (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10017812B2 (en) | 2010-05-18 | 2018-07-10 | Natera, Inc. | Methods for non-invasive prenatal ploidy calling |
| US20130123120A1 (en) | 2010-05-18 | 2013-05-16 | Natera, Inc. | Highly Multiplex PCR Methods and Compositions |
| WO2015164432A1 (en) | 2014-04-21 | 2015-10-29 | Natera, Inc. | Detecting mutations and ploidy in chromosomal segments |
| US20190106751A1 (en) | 2016-04-15 | 2019-04-11 | Natera, Inc. | Methods for lung cancer detection |
| JP2019534016A (ja) * | 2016-11-02 | 2019-11-28 | ザ メディカル カレッジ オブ ウィスコンシン，インコーポレイテッドＴｈｅ Ｍｅｄｉｃａｌ Ｃｏｌｌｅｇｅ ｏｆ Ｗｉｓｃｏｎｓｉｎ， Ｉｎｃ． | 全および特異的な無細胞ｄｎａを使用しリスクを評価するための方法 |
| GB201618485D0 (en) | 2016-11-02 | 2016-12-14 | Ucl Business Plc | Method of detecting tumour recurrence |
| CA3090426A1 (en) | 2018-04-14 | 2019-10-17 | Natera, Inc. | Methods for cancer detection and monitoring by means of personalized detection of circulating tumor dna |
| BR112020027023A2 (pt) | 2018-07-03 | 2021-04-06 | Natera, Inc. | Métodos para detecção de dna livre de células derivado de doador |
| EP3824470A1 (en) | 2018-07-17 | 2021-05-26 | Natera, Inc. | Methods and systems for calling ploidy states using a neural network |
| CA3134519A1 (en) * | 2019-04-15 | 2020-10-22 | Natera, Inc. | Improved liquid biopsy using size selection |
| CA3180334A1 (en) * | 2020-05-29 | 2021-12-02 | Natera, Inc. | Methods for detection of donor-derived cell-free dna |
-
2022
- 2022-02-24 CN CN202280019708.4A patent/CN116964223A/zh active Pending
- 2022-02-24 JP JP2023550565A patent/JP2024507536A/ja active Pending
- 2022-02-24 CA CA3211540A patent/CA3211540A1/en active Pending
- 2022-02-24 EP EP22709914.0A patent/EP4298248A1/en active Pending
- 2022-02-24 AU AU2022226186A patent/AU2022226186A1/en active Pending
- 2022-02-24 BR BR112023016896A patent/BR112023016896A2/pt unknown
- 2022-02-24 WO PCT/US2022/017707 patent/WO2022182878A1/en active Application Filing
Also Published As
| Publication number | Publication date |
|---|---|
| EP4298248A1 (en) | 2024-01-03 |
| CA3211540A1 (en) | 2022-09-01 |
| CN116964223A (zh) | 2023-10-27 |
| WO2022182878A1 (en) | 2022-09-01 |
| AU2022226186A1 (en) | 2023-09-07 |
| JP2024507536A (ja) | 2024-02-20 |
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