BR112023014382A2 - Estrutura de transcrição gênica, sistema vetorial, método de edição da sequência do genoma e aplicação - Google Patents

Estrutura de transcrição gênica, sistema vetorial, método de edição da sequência do genoma e aplicação

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Publication number
BR112023014382A2
BR112023014382A2 BR112023014382A BR112023014382A BR112023014382A2 BR 112023014382 A2 BR112023014382 A2 BR 112023014382A2 BR 112023014382 A BR112023014382 A BR 112023014382A BR 112023014382 A BR112023014382 A BR 112023014382A BR 112023014382 A2 BR112023014382 A2 BR 112023014382A2
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sequence
editing method
genome
gene transcription
application
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BR112023014382A
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English (en)
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Shuanghong Peng
Yunpeng Sui
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Shuanghong Peng
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Publication of BR112023014382A2 publication Critical patent/BR112023014382A2/pt

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    • C12N15/09—Recombinant DNA-technology
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
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Abstract

estrutura de transcrição gênica,sistema vetorial, método de edição da sequência do genoma e aplicação. a presente invenção refere-se a uma estrutura de transcrição do gene, um sistema de vetor, um método de edição de sequência do genoma e respetiva aplicação. a estrutura de transcrição gênica é baseada em um mecanismo de retrotransposição eucariótica e pode ser mediada por uma via de dna, rna ou rnp, e compreende a sequência de produção de transcrição que compreende uma ou mais sequência a montante do sítio alvo, uma sequência a ser inserida e uma sequência a jusante do sítio alvo, um ou mais sine (elemento(s)), uma ou mais line (elemento(s)), uma ou mais sequência(s) de codificação da orf1p e/ou uma ou mais sequência(s) de codificação da orf2p. de acordo com o método de edição de genes, com base na premissa de que sistemas ou substâncias estranhas não são introduzidos tanto quanto possível e quebras de fita dupla não são geradas, transferindo o sistema de edição de genes para o núcleo ou citoplasma através de uma via de dna, rna ou rnp (rna ou rnp é transferido do citoplasma para o núcleo através da mediação orf1p e/ou orf2p), o fragmento alvo é inserido em um local designado em um genoma ou o fragmento designado no genoma é excluído ou substituído, e alta precisão de direcionamento é alcançada.
BR112023014382A 2021-01-22 2021-12-01 Estrutura de transcrição gênica, sistema vetorial, método de edição da sequência do genoma e aplicação BR112023014382A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CN202110089068.1A CN112708636A (zh) 2021-01-22 2021-01-22 基因转录框架、载体系统、基因组序列编辑方法及应用
PCT/CN2021/134710 WO2022156378A1 (zh) 2021-01-22 2021-12-01 基因转录框架、载体系统、基因组序列编辑方法及应用

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BR112023014382A2 true BR112023014382A2 (pt) 2023-10-31

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EP (1) EP4282968A1 (pt)
JP (1) JP2024504592A (pt)
KR (1) KR20230135630A (pt)
CN (1) CN112708636A (pt)
AU (1) AU2021422067A1 (pt)
BR (1) BR112023014382A2 (pt)
CA (1) CA3206081A1 (pt)
MX (1) MX2023008635A (pt)
WO (1) WO2022156378A1 (pt)
ZA (1) ZA202308025B (pt)

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CN112481259B (zh) * 2020-11-24 2022-09-16 南昌大学 两种甘薯U6基因启动子IbU6的克隆与应用
CN115044583A (zh) * 2022-03-21 2022-09-13 隋云鹏 用于基因编辑的rna框架和基因编辑方法
CN115312121B (zh) * 2022-09-29 2023-03-24 北京齐碳科技有限公司 靶基因位点检测方法、装置、设备及计算机存储介质

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CN102080101B (zh) * 2010-11-25 2013-01-09 北京济福霖生物技术有限公司 一种定点整合外源基因的方法
WO2016030501A1 (en) * 2014-08-28 2016-03-03 Centre National De La Recherche Scientifique - Cnrs - Synthetic alu-retrotransposon vectors for gene therapy
US11339427B2 (en) * 2016-02-12 2022-05-24 Jumpcode Genomics, Inc. Method for target specific RNA transcription of DNA sequences
WO2020047124A1 (en) * 2018-08-28 2020-03-05 Flagship Pioneering, Inc. Methods and compositions for modulating a genome
CA3116762A1 (en) * 2018-10-19 2020-04-23 Board Of Regents, The University Of Texas System Engineered long interspersed element (line) transposons and methods of use thereof
CN112210573B (zh) * 2020-10-14 2024-02-06 浙江大学 一种用基因编辑改造原代细胞的dna模板及定点插入方法

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ZA202308025B (en) 2024-03-27
KR20230135630A (ko) 2023-09-25
CN112708636A (zh) 2021-04-27
MX2023008635A (es) 2023-10-17
AU2021422067A1 (en) 2023-09-07
EP4282968A1 (en) 2023-11-29
WO2022156378A1 (zh) 2022-07-28
JP2024504592A (ja) 2024-02-01
CA3206081A1 (en) 2022-07-28

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