BR112023003972A2 - Complexos de um módulo de reconhecimento de sequência de ácido nucleico ligado a uma desaminase e de um fragmento amino-terminal de um módulo de reconhecimento de sequência de ácido nucleico, uma desaminase e um fragmento carboxi-terminal de um módulo de reconhecimento de sequência de ácido nucleico ligado ao mesmo, ácido nucleico, vetor, e, método para alterar um sítio alvo de um ácido desoxirribonucleico de fita dupla de uma célula - Google Patents
Complexos de um módulo de reconhecimento de sequência de ácido nucleico ligado a uma desaminase e de um fragmento amino-terminal de um módulo de reconhecimento de sequência de ácido nucleico, uma desaminase e um fragmento carboxi-terminal de um módulo de reconhecimento de sequência de ácido nucleico ligado ao mesmo, ácido nucleico, vetor, e, método para alterar um sítio alvo de um ácido desoxirribonucleico de fita dupla de uma célulaInfo
- Publication number
- BR112023003972A2 BR112023003972A2 BR112023003972A BR112023003972A BR112023003972A2 BR 112023003972 A2 BR112023003972 A2 BR 112023003972A2 BR 112023003972 A BR112023003972 A BR 112023003972A BR 112023003972 A BR112023003972 A BR 112023003972A BR 112023003972 A2 BR112023003972 A2 BR 112023003972A2
- Authority
- BR
- Brazil
- Prior art keywords
- acid sequence
- nucleic acid
- recognition module
- sequence recognition
- deaminase
- Prior art date
Links
- 150000007523 nucleic acids Chemical group 0.000 title abstract 10
- 108091028043 Nucleic acid sequence Proteins 0.000 title abstract 6
- 108020004414 DNA Proteins 0.000 title abstract 5
- 102000053602 DNA Human genes 0.000 title abstract 4
- 239000012634 fragment Substances 0.000 title abstract 4
- 108020004707 nucleic acids Proteins 0.000 title abstract 4
- 102000039446 nucleic acids Human genes 0.000 title abstract 4
- 239000002253 acid Substances 0.000 title abstract 2
- 238000000034 method Methods 0.000 title abstract 2
- 125000003275 alpha amino acid group Chemical group 0.000 abstract 8
- 102100026846 Cytidine deaminase Human genes 0.000 abstract 4
- 108010031325 Cytidine deaminase Proteins 0.000 abstract 4
- 150000001413 amino acids Chemical class 0.000 abstract 2
- 125000000539 amino acid group Chemical group 0.000 abstract 1
- 230000004048 modification Effects 0.000 abstract 1
- 238000012986 modification Methods 0.000 abstract 1
- 239000002773 nucleotide Substances 0.000 abstract 1
- 125000003729 nucleotide group Chemical group 0.000 abstract 1
- 230000009437 off-target effect Effects 0.000 abstract 1
- 102000004169 proteins and genes Human genes 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
- C12N15/86—Viral vectors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y305/00—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5)
- C12Y305/04—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5) in cyclic amidines (3.5.4)
- C12Y305/04005—Cytidine deaminase (3.5.4.5)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
- C12N2750/00011—Details
- C12N2750/14011—Parvoviridae
- C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
- C12N2750/14141—Use of virus, viral particle or viral elements as a vector
- C12N2750/14143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Crystallography & Structural Chemistry (AREA)
- Virology (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2020149419 | 2020-09-04 | ||
| PCT/JP2021/032689 WO2022050413A1 (ja) | 2020-09-04 | 2021-09-06 | 小型化シチジンデアミナーゼを含む二本鎖ｄｎａの改変用複合体 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| BR112023003972A2 true BR112023003972A2 (pt) | 2023-04-18 |
Family
ID=80492029
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BR112023003972A BR112023003972A2 (pt) | 2020-09-04 | 2021-09-06 | Complexos de um módulo de reconhecimento de sequência de ácido nucleico ligado a uma desaminase e de um fragmento amino-terminal de um módulo de reconhecimento de sequência de ácido nucleico, uma desaminase e um fragmento carboxi-terminal de um módulo de reconhecimento de sequência de ácido nucleico ligado ao mesmo, ácido nucleico, vetor, e, método para alterar um sítio alvo de um ácido desoxirribonucleico de fita dupla de uma célula |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US20230323335A1 (https=) |
| EP (1) | EP4209589A4 (https=) |
| JP (1) | JPWO2022050413A1 (https=) |
| KR (1) | KR20230061474A (https=) |
| CN (1) | CN116134141A (https=) |
| AU (1) | AU2021336262A1 (https=) |
| BR (1) | BR112023003972A2 (https=) |
| CA (1) | CA3194019A1 (https=) |
| WO (1) | WO2022050413A1 (https=) |
Family Cites Families (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2003087341A2 (en) | 2002-01-23 | 2003-10-23 | The University Of Utah Research Foundation | Targeted chromosomal mutagenesis using zinc finger nucleases |
| EP2345731B1 (en) | 2003-09-30 | 2015-10-21 | The Trustees of the University of Pennsylvania | Adeno-associated virus (AAV) clades, sequences, vectors containing same, and uses thereof |
| WO2010012077A1 (en) | 2008-07-28 | 2010-02-04 | Mount Sinai Hospital | Compositions, methods and kits for reprogramming somatic cells |
| WO2011072246A2 (en) | 2009-12-10 | 2011-06-16 | Regents Of The University Of Minnesota | Tal effector-mediated dna modification |
| JP2013128413A (ja) | 2010-03-11 | 2013-07-04 | Kyushu Univ | Ｐｐｒモチーフを利用したｒｎａ結合性蛋白質の改変方法 |
| AU2013266968B2 (en) | 2012-05-25 | 2017-06-29 | Emmanuelle CHARPENTIER | Methods and compositions for RNA-directed target DNA modification and for RNA-directed modulation of transcription |
| EP3004339B1 (en) * | 2013-05-29 | 2021-07-07 | Cellectis | New compact scaffold of cas9 in the type ii crispr system |
| EP3115457B1 (en) | 2014-03-05 | 2019-10-02 | National University Corporation Kobe University | Genomic sequence modification method for specifically converting nucleic acid bases of targeted dna sequence, and molecular complex for use in same |
| US11542496B2 (en) * | 2017-03-10 | 2023-01-03 | President And Fellows Of Harvard College | Cytosine to guanine base editor |
| KR20240116572A (ko) * | 2017-03-23 | 2024-07-29 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | 핵산 프로그램가능한 dna 결합 단백질을 포함하는 핵염기 편집제 |
| EP3630849A4 (en) * | 2017-05-25 | 2021-01-13 | The General Hospital Corporation | BIPARTITE BASIC EDITOR (BBE) AND TYPE II-C-CAS9 ZINC FINGER EDITOR ARCHITECTURES |
| KR102287880B1 (ko) | 2018-03-26 | 2021-08-09 | 고쿠리츠다이가쿠호진 고베다이가쿠 | 세포에서 이중 가닥 dna의 표적 부위를 변형시키기 위한 방법 |
| WO2020091069A1 (ja) * | 2018-11-01 | 2020-05-07 | 国立大学法人東京大学 | ２分割されたＣｐｆ１タンパク質 |
| AU2020215730A1 (en) * | 2019-01-31 | 2021-07-29 | Beam Therapeutics Inc. | Nucleobase editors having reduced non-target deamination and assays for characterizing nucleobase editors |
| JP7693552B2 (ja) * | 2019-02-13 | 2025-06-17 | ビーム セラピューティクス インク． | アデノシンデアミナーゼ塩基エディターおよびそれを用いて標的配列中の核酸塩基を改変する方法 |
| JP7234711B2 (ja) | 2019-03-14 | 2023-03-08 | 富士フイルムビジネスイノベーション株式会社 | メッセージ通知装置、コンテンツ管理システム及びプログラム |
-
2021
- 2021-09-06 CN CN202180054915.9A patent/CN116134141A/zh active Pending
- 2021-09-06 EP EP21864464.9A patent/EP4209589A4/en active Pending
- 2021-09-06 KR KR1020237011179A patent/KR20230061474A/ko active Pending
- 2021-09-06 AU AU2021336262A patent/AU2021336262A1/en active Pending
- 2021-09-06 JP JP2022546998A patent/JPWO2022050413A1/ja active Pending
- 2021-09-06 WO PCT/JP2021/032689 patent/WO2022050413A1/ja not_active Ceased
- 2021-09-06 US US18/044,047 patent/US20230323335A1/en active Pending
- 2021-09-06 BR BR112023003972A patent/BR112023003972A2/pt unknown
- 2021-09-06 CA CA3194019A patent/CA3194019A1/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| EP4209589A4 (en) | 2024-09-25 |
| JPWO2022050413A1 (https=) | 2022-03-10 |
| CN116134141A (zh) | 2023-05-16 |
| AU2021336262A9 (en) | 2024-09-05 |
| CA3194019A1 (en) | 2022-03-10 |
| AU2021336262A1 (en) | 2023-05-18 |
| EP4209589A1 (en) | 2023-07-12 |
| WO2022050413A1 (ja) | 2022-03-10 |
| KR20230061474A (ko) | 2023-05-08 |
| US20230323335A1 (en) | 2023-10-12 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Wower et al. | Binding and cross‐linking of tmRNA to ribosomal protein S1, on and off the Escherichia coli ribosome | |
| Schaeffer et al. | Protein‐protein interactions in the eubacterial replisome | |
| BR9915543A (pt) | Resistência a doenças por plantas mediada por patogenicidas moleculares | |
| Chiang et al. | In vivo tagging and characterization of S-glutathionylated proteins by a chemoenzymatic method | |
| Xu et al. | Molecular basis of transcriptional fidelity and DNA lesion-induced transcriptional mutagenesis | |
| Sekine et al. | Structural basis of transcription by bacterial and eukaryotic RNA polymerases | |
| Brown et al. | Ribosome “skipping”:“stop-carry on” or “stopgo” translation | |
| Matveenko et al. | Utility of the phenacyl protecting group in traceless protein semisynthesis through ligation–Desulfurization chemistry | |
| BR112023003972A2 (pt) | Complexos de um módulo de reconhecimento de sequência de ácido nucleico ligado a uma desaminase e de um fragmento amino-terminal de um módulo de reconhecimento de sequência de ácido nucleico, uma desaminase e um fragmento carboxi-terminal de um módulo de reconhecimento de sequência de ácido nucleico ligado ao mesmo, ácido nucleico, vetor, e, método para alterar um sítio alvo de um ácido desoxirribonucleico de fita dupla de uma célula | |
| Pugh et al. | Ferroplasma acidarmanus RPA2 facilitates efficient unwinding of forked DNA substrates by monomers of FacXPD helicase | |
| Bhangu et al. | Arrangement of messenger RNA on Escherichia coli ribosomes with respect to 10 16S rRNA crosslinking sites | |
| CN120272474A (zh) | 与核酸抗凝剂有关的组合物和方法 | |
| Nunes-Düby et al. | Single base-pair precision and structural rigidity in a small IHF-induced DNA loop | |
| Graifer et al. | Photoactivatable RNA derivatives as tools for studying the structural and functional organization of complex cellular ribonucleoprotein machineries | |
| Diefenderfer et al. | Reliable expression and purification of highly insoluble transmembrane domains | |
| Zenkova et al. | A Novel Approach to Introduce Site‐Directed Specific Cross‐Links Within RNA‐Protein Complexes: Application to the Escherichia Coli Threonyl‐tRNA Synthetase/Translational Operator Complex | |
| Manhart et al. | Identification of subunit binding positions on a model fork and displacements that occur during sequential assembly of the Escherichia coli primosome | |
| Thawani et al. | Structural and biochemical studies of mobile retrotransposon proteins in action | |
| Xie | A unified model of nucleic acid unwinding by the ribosome and the hexameric and monomeric DNA helicases | |
| Gopanenko et al. | Exploring human 40S ribosomal proteins binding to the 18S rRNA fragment containing major 3′-terminal domain | |
| WO2024059719A2 (en) | Compositions for preventing repetitive addition of switching oligonucleotides and nonspecific primer extension during cdna synthesis and methods of use thereof | |
| Shen et al. | High yield recombinant expression and purification of oncogenic NSD1, NSD2, and NSD3 with human influenza hemagglutinin tag | |
| Gaudin et al. | The tRNA-like domains of E. coli and A. aeolicus transfer–messenger RNA: structural and functional studies | |
| Shikalov et al. | The σ24 subunit of Escherichia coli RNA polymerase can induce transcriptional pausing in vitro | |
| Coman et al. | Dual functions, clamp opening and primer-template recognition, define a key clamp loader subunit |