BR112022020899A2 - Método para tratamento de um meio de cultura celular, meio de cultura celular, uso do meio e método para produzir uma proteína recombinante - Google Patents

Método para tratamento de um meio de cultura celular, meio de cultura celular, uso do meio e método para produzir uma proteína recombinante

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Publication number
BR112022020899A2
BR112022020899A2 BR112022020899A BR112022020899A BR112022020899A2 BR 112022020899 A2 BR112022020899 A2 BR 112022020899A2 BR 112022020899 A BR112022020899 A BR 112022020899A BR 112022020899 A BR112022020899 A BR 112022020899A BR 112022020899 A2 BR112022020899 A2 BR 112022020899A2
Authority
BR
Brazil
Prior art keywords
cell culture
culture media
medium
producing
treatment
Prior art date
Application number
BR112022020899A
Other languages
English (en)
Inventor
Popp Oliver
Ken Shiratori Masaru
Original Assignee
Genentech Inc
Hoffmann La Roche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genentech Inc, Hoffmann La Roche filed Critical Genentech Inc
Publication of BR112022020899A2 publication Critical patent/BR112022020899A2/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0681—Cells of the genital tract; Non-germinal cells from gonads
    • C12N5/0682—Cells of the female genital tract, e.g. endometrium; Non-germinal cells from ovaries, e.g. ovarian follicle cells
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/32—Amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2523/00—Culture process characterised by temperature

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Reproductive Health (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

MÉTODO PARA TRATAMENTO DE UM MEIO DE CULTURA CELULAR, MEIO DE CULTURA CELULAR, USO DO MEIO E MÉTODO PARA PRODUZIR UMA PROTEÍNA RECOMBINANTE. A divulgação refere-se a métodos para manipulação e suplementação de meio de cultura celular para melhorar o desempenho do processo em sistemas de expressão recombinante eucarióticos.
BR112022020899A 2020-04-15 2021-04-14 Método para tratamento de um meio de cultura celular, meio de cultura celular, uso do meio e método para produzir uma proteína recombinante BR112022020899A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US202063010536P 2020-04-15 2020-04-15
PCT/EP2021/059690 WO2021209512A1 (en) 2020-04-15 2021-04-14 Method for medium treatment before inoculation

Publications (1)

Publication Number Publication Date
BR112022020899A2 true BR112022020899A2 (pt) 2022-12-27

Family

ID=75674775

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112022020899A BR112022020899A2 (pt) 2020-04-15 2021-04-14 Método para tratamento de um meio de cultura celular, meio de cultura celular, uso do meio e método para produzir uma proteína recombinante

Country Status (12)

Country Link
US (1) US20230279342A1 (pt)
EP (1) EP4136212A1 (pt)
JP (1) JP2023521236A (pt)
KR (1) KR20230002642A (pt)
CN (1) CN115397971A (pt)
AU (1) AU2021257609A1 (pt)
BR (1) BR112022020899A2 (pt)
CA (1) CA3175256A1 (pt)
IL (1) IL297191A (pt)
MX (1) MX2022012829A (pt)
TW (1) TW202204602A (pt)
WO (1) WO2021209512A1 (pt)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2023157970A1 (ja) * 2022-02-21 2023-08-24 中外製薬株式会社 灌流培養方法
CN115747138B (zh) * 2022-12-01 2024-03-08 苏州依科赛生物科技股份有限公司 一种hek 293细胞培养基及其应用

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL87737A (en) 1987-09-11 1993-08-18 Genentech Inc Method for culturing polypeptide factor dependent vertebrate recombinant cells
US6030945A (en) 1996-01-09 2000-02-29 Genentech, Inc. Apo-2 ligand

Also Published As

Publication number Publication date
AU2021257609A1 (en) 2022-11-10
JP2023521236A (ja) 2023-05-23
TW202204602A (zh) 2022-02-01
CN115397971A (zh) 2022-11-25
CA3175256A1 (en) 2021-10-21
WO2021209512A1 (en) 2021-10-21
EP4136212A1 (en) 2023-02-22
IL297191A (en) 2022-12-01
US20230279342A1 (en) 2023-09-07
KR20230002642A (ko) 2023-01-05
MX2022012829A (es) 2022-11-07

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