BR112022015920A2 - Método para determinar a razão de partículas de aav e uso do tempo de relaxação - Google Patents
Método para determinar a razão de partículas de aav e uso do tempo de relaxaçãoInfo
- Publication number
- BR112022015920A2 BR112022015920A2 BR112022015920A BR112022015920A BR112022015920A2 BR 112022015920 A2 BR112022015920 A2 BR 112022015920A2 BR 112022015920 A BR112022015920 A BR 112022015920A BR 112022015920 A BR112022015920 A BR 112022015920A BR 112022015920 A2 BR112022015920 A2 BR 112022015920A2
- Authority
- BR
- Brazil
- Prior art keywords
- viral particles
- ratio
- empty
- relaxation time
- charged
- Prior art date
Links
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N24/00—Investigating or analyzing materials by the use of nuclear magnetic resonance, electron paramagnetic resonance or other spin effects
- G01N24/08—Investigating or analyzing materials by the use of nuclear magnetic resonance, electron paramagnetic resonance or other spin effects by using nuclear magnetic resonance
- G01N24/087—Structure determination of a chemical compound, e.g. of a biomolecule such as a protein
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/62—Detectors specially adapted therefor
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N24/00—Investigating or analyzing materials by the use of nuclear magnetic resonance, electron paramagnetic resonance or other spin effects
- G01N24/08—Investigating or analyzing materials by the use of nuclear magnetic resonance, electron paramagnetic resonance or other spin effects by using nuclear magnetic resonance
- G01N24/085—Analysis of materials for the purpose of controlling industrial production systems
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/18—Ion-exchange chromatography
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N30/06—Preparation
- G01N30/14—Preparation by elimination of some components
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01R—MEASURING ELECTRIC VARIABLES; MEASURING MAGNETIC VARIABLES
- G01R33/00—Arrangements or instruments for measuring magnetic variables
- G01R33/20—Arrangements or instruments for measuring magnetic variables involving magnetic resonance
- G01R33/44—Arrangements or instruments for measuring magnetic variables involving magnetic resonance using nuclear magnetic resonance [NMR]
- G01R33/448—Relaxometry, i.e. quantification of relaxation times or spin density
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01R—MEASURING ELECTRIC VARIABLES; MEASURING MAGNETIC VARIABLES
- G01R33/00—Arrangements or instruments for measuring magnetic variables
- G01R33/20—Arrangements or instruments for measuring magnetic variables involving magnetic resonance
- G01R33/44—Arrangements or instruments for measuring magnetic variables involving magnetic resonance using nuclear magnetic resonance [NMR]
- G01R33/46—NMR spectroscopy
- G01R33/465—NMR spectroscopy applied to biological material, e.g. in vitro testing
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
- C12N2750/00011—Details
- C12N2750/14011—Parvoviridae
- C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
- C12N2750/14151—Methods of production or purification of viral material
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N2030/022—Column chromatography characterised by the kind of separation mechanism
- G01N2030/027—Liquid chromatography
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
- G01N2030/8809—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample
- G01N2030/8813—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials
- G01N2030/8831—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials involving peptides or proteins
Landscapes
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- High Energy & Nuclear Physics (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Analytical Chemistry (AREA)
- Organic Chemistry (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Condensed Matter Physics & Semiconductors (AREA)
- Molecular Biology (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biophysics (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Virology (AREA)
- General Engineering & Computer Science (AREA)
- Crystallography & Structural Chemistry (AREA)
- Microbiology (AREA)
- Peptides Or Proteins (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Magnetic Resonance Imaging Apparatus (AREA)
- Investigating Or Analyzing Materials By The Use Of Magnetic Means (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Compounds Of Iron (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
MÉTODO PARA DETERMINAR A RAZÃO DE PARTÍCULAS DE AAV E USO DO TEMPO DE RELAXAÇÃO. A presente invenção baseia-se, pelo menos em parte, na descoberta de que o tempo de relaxação de rotação magnética nuclear transversal T2 e a taxa de relaxação de rotação magnética nuclear transversal R2, respectivamente, de prótons de moléculas de água em uma solução aquosa compreendendo partículas virais dependem do estado de carregamento (cheio versus vazio) da partícula viral. Assim, um aspecto da presente invenção é um método para determinar a razão de partículas virais carregadas para partículas virais vazias em uma amostra, compreendendo as etapas de determinar um parâmetro de ressonância magnética nuclear (RMN) relacionado aos prótons das moléculas de água presentes em uma solução aquosa compreendendo uma mistura de partículas virais carregadas e vazias aplicando uma medição de RMN à solução, e determinar a razão de partículas virais carregadas para partículas virais vazias com o parâmetro de RMN determinado na etapa anterior com base em uma função de calibração.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP20157222 | 2020-02-13 | ||
PCT/EP2021/053451 WO2021160799A1 (en) | 2020-02-13 | 2021-02-12 | Method for determining the loading state of an aav particle by nuclear magnetic resonance relaxometry |
Publications (1)
Publication Number | Publication Date |
---|---|
BR112022015920A2 true BR112022015920A2 (pt) | 2022-10-04 |
Family
ID=69699738
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
BR112022015920A BR112022015920A2 (pt) | 2020-02-13 | 2021-02-12 | Método para determinar a razão de partículas de aav e uso do tempo de relaxação |
Country Status (11)
Country | Link |
---|---|
US (2) | US20230047531A1 (pt) |
EP (2) | EP4103937A1 (pt) |
JP (2) | JP2023513347A (pt) |
KR (1) | KR20220137672A (pt) |
CN (2) | CN115087861A (pt) |
AU (1) | AU2021219957A1 (pt) |
BR (1) | BR112022015920A2 (pt) |
CA (1) | CA3168607A1 (pt) |
IL (1) | IL295143A (pt) |
MX (1) | MX2022009642A (pt) |
WO (2) | WO2021160787A1 (pt) |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN117179737B (zh) * | 2023-11-06 | 2024-02-20 | 哈尔滨医科大学 | 多核素同步一体化磁共振成像核素定量系统及其使用方法 |
Family Cites Families (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP6061853B2 (ja) | 2010-07-30 | 2017-01-18 | メディミューン,エルエルシー | 活性ポリペプチドまたは免疫複合体の精製方法 |
US10267754B2 (en) | 2013-04-12 | 2019-04-23 | University Of Maryland, Baltimore | Assessing biopharmaceutical aggregation using magnetic resonance relaxometry |
PT3102595T (pt) | 2014-02-06 | 2019-01-11 | Hoffmann La Roche | Proteínas de fusão de interleucina-2 e suas utilizações |
US11346908B2 (en) | 2016-12-02 | 2022-05-31 | University Of Maryland, Baltimore | Solvent nuclear magnetic resonance for noninvasive inspection of particle-containing products |
BR112019017329A2 (pt) | 2017-04-03 | 2020-04-14 | Hoffmann La Roche | imunoconjugados, um ou mais polinucleotídeos e vetores, métodos para a produção de um imunoconjugado, tratamento de uma doença e para a estimulação do sistema imunológico, composição, uso do imunoconjugado, invenção e usos da composição |
GB201711481D0 (en) | 2017-07-17 | 2017-08-30 | Ucb Biopharma Sprl | Protein purification |
-
2021
- 2021-02-12 EP EP21704554.1A patent/EP4103937A1/en active Pending
- 2021-02-12 AU AU2021219957A patent/AU2021219957A1/en active Pending
- 2021-02-12 EP EP21704794.3A patent/EP4103938A1/en active Pending
- 2021-02-12 WO PCT/EP2021/053429 patent/WO2021160787A1/en unknown
- 2021-02-12 IL IL295143A patent/IL295143A/en unknown
- 2021-02-12 CN CN202180014126.2A patent/CN115087861A/zh active Pending
- 2021-02-12 KR KR1020227029251A patent/KR20220137672A/ko unknown
- 2021-02-12 CN CN202180014521.0A patent/CN115135999A/zh active Pending
- 2021-02-12 JP JP2022548831A patent/JP2023513347A/ja active Pending
- 2021-02-12 JP JP2022548985A patent/JP2023513903A/ja active Pending
- 2021-02-12 CA CA3168607A patent/CA3168607A1/en active Pending
- 2021-02-12 WO PCT/EP2021/053451 patent/WO2021160799A1/en unknown
- 2021-02-12 MX MX2022009642A patent/MX2022009642A/es unknown
- 2021-02-12 BR BR112022015920A patent/BR112022015920A2/pt unknown
-
2022
- 2022-08-11 US US17/886,357 patent/US20230047531A1/en active Pending
- 2022-08-12 US US17/886,622 patent/US20220381750A1/en active Pending
Also Published As
Publication number | Publication date |
---|---|
WO2021160799A1 (en) | 2021-08-19 |
AU2021219957A1 (en) | 2022-08-04 |
EP4103937A1 (en) | 2022-12-21 |
IL295143A (en) | 2022-09-01 |
JP2023513347A (ja) | 2023-03-30 |
CA3168607A1 (en) | 2021-08-19 |
WO2021160787A1 (en) | 2021-08-19 |
CN115135999A (zh) | 2022-09-30 |
US20230047531A1 (en) | 2023-02-16 |
US20220381750A1 (en) | 2022-12-01 |
CN115087861A (zh) | 2022-09-20 |
KR20220137672A (ko) | 2022-10-12 |
MX2022009642A (es) | 2022-09-07 |
JP2023513903A (ja) | 2023-04-04 |
EP4103938A1 (en) | 2022-12-21 |
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