BR112022012853A2 - Cepa modificada por lipase - Google Patents

Cepa modificada por lipase

Info

Publication number
BR112022012853A2
BR112022012853A2 BR112022012853A BR112022012853A BR112022012853A2 BR 112022012853 A2 BR112022012853 A2 BR 112022012853A2 BR 112022012853 A BR112022012853 A BR 112022012853A BR 112022012853 A BR112022012853 A BR 112022012853A BR 112022012853 A2 BR112022012853 A2 BR 112022012853A2
Authority
BR
Brazil
Prior art keywords
host cell
modified
lipase
retinyl acetate
producing
Prior art date
Application number
BR112022012853A
Other languages
English (en)
Inventor
McMahon Jenna
Irsan Kooi Elvin
Wu Liang
Marcel De Jong René
Kanubhai Vyas Valmik
Louis Houston Peter
Original Assignee
Dsm Ip Assets Bv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dsm Ip Assets Bv filed Critical Dsm Ip Assets Bv
Publication of BR112022012853A2 publication Critical patent/BR112022012853A2/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P23/00—Preparation of compounds containing a cyclohexene ring having an unsaturated side chain containing at least ten carbon atoms bound by conjugated double bonds, e.g. carotenes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/44—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving esterase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/916—Hydrolases (3) acting on ester bonds (3.1), e.g. phosphatases (3.1.3), phospholipases C or phospholipases D (3.1.4)
    • G01N2333/918—Carboxylic ester hydrolases (3.1.1)
    • G01N2333/92—Triglyceride splitting, e.g. by means of lipase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Cosmetics (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

CEPA MODIFICADA POR LIPASE. A presente invenção está relacionada a uma célula hospedeira produtora de retinoides, particularmente levedura oleaginosa, modificada de modo que a porcentagem de acetato de retinila com base no total de retinoides produzidos por tal célula hospedeira seja aumentada durante a fermentação usando óleos de triglicerídeos, como, por exemplo, óleo vegetal, como fonte de carbono, em que a atividade de certas hidrolases ou transferases endógenas envolvidas em conversões indesejadas de retinol ou acetato de retinila é reduzida ou abolida. Particularmente, tal célula hospedeira modificada pode ser útil em um processo biotecnológico para produção de vitamina A.
BR112022012853A 2019-12-30 2020-12-18 Cepa modificada por lipase BR112022012853A2 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
CH17162019 2019-12-30
EP20187829 2020-07-27
PCT/EP2020/087018 WO2021136689A1 (en) 2019-12-30 2020-12-18 Lipase-modified strain

Publications (1)

Publication Number Publication Date
BR112022012853A2 true BR112022012853A2 (pt) 2022-09-20

Family

ID=74106048

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112022012853A BR112022012853A2 (pt) 2019-12-30 2020-12-18 Cepa modificada por lipase

Country Status (7)

Country Link
US (1) US20230049760A1 (pt)
EP (1) EP4085132A1 (pt)
JP (1) JP2023507891A (pt)
CN (1) CN114901816A (pt)
BR (1) BR112022012853A2 (pt)
CA (1) CA3166033A1 (pt)
WO (1) WO2021136689A1 (pt)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2022023173A1 (en) * 2020-07-27 2022-02-03 Dsm Ip Assets B.V. Reduction of fatty acid retinyl ester formation
WO2022090549A1 (en) * 2020-10-30 2022-05-05 Dsm Ip Assets B.V. In situ two-phase extraction system
WO2023275212A1 (en) * 2021-07-01 2023-01-05 Dsm Ip Assets B.V. Sustainable production of retinyl fatty esters
WO2023067030A1 (en) 2021-10-19 2023-04-27 Dsm Ip Assets B.V. Retinoid production

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2006227165B2 (en) 2005-03-18 2011-11-10 Microbia, Inc. Production of carotenoids in oleaginous yeast and fungi
CN100424170C (zh) * 2005-10-11 2008-10-08 北京化工大学 脂肪酶、其基因、产生该酶的亚罗解脂酵母及其应用
US8691555B2 (en) 2006-09-28 2014-04-08 Dsm Ip Assests B.V. Production of carotenoids in oleaginous yeast and fungi
CN101200740A (zh) * 2007-12-18 2008-06-18 北京化工大学 一种用脂肪酶催化制备维生素a脂肪酸酯的方法
EP2294193A4 (en) * 2008-04-10 2012-11-28 Dsm Ip Assets Bv PRODUCTION OF CAROTENOIDS IN YEAST AND OLEAGINOUS FUNGI
CN107532141A (zh) 2015-04-21 2018-01-02 帝斯曼知识产权资产管理有限公司 香叶基香叶基焦磷酸合酶
KR20230170126A (ko) * 2016-09-30 2023-12-18 더 리젠츠 오브 더 유니버시티 오브 캘리포니아 Rna-가이드된 핵산 변형 효소 및 이의 사용 방법
WO2019057998A1 (en) 2017-09-25 2019-03-28 Dsm Ip Assets B.V. RETINOL PRODUCTION
CN111107832A (zh) 2017-09-25 2020-05-05 帝斯曼知识产权资产管理有限公司 视黄酯的生产
WO2019058000A1 (en) 2017-09-25 2019-03-28 Dsm Ip Assets B.V. BIOSYNTHESIS OF RETINOIDS
CN111107833A (zh) * 2017-09-25 2020-05-05 帝斯曼知识产权资产管理有限公司 反式视黄醛的生产

Also Published As

Publication number Publication date
EP4085132A1 (en) 2022-11-09
CN114901816A (zh) 2022-08-12
US20230049760A1 (en) 2023-02-16
WO2021136689A1 (en) 2021-07-08
JP2023507891A (ja) 2023-02-28
CA3166033A1 (en) 2021-07-08

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