BR112022003992A2 - Sequência sinal, ácido nucleico, construto de ácido nucleico, vetor de expressão, microrganismo recombinante e método para produzir uma proteína crm197 - Google Patents

Sequência sinal, ácido nucleico, construto de ácido nucleico, vetor de expressão, microrganismo recombinante e método para produzir uma proteína crm197

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Publication number
BR112022003992A2
BR112022003992A2 BR112022003992A BR112022003992A BR112022003992A2 BR 112022003992 A2 BR112022003992 A2 BR 112022003992A2 BR 112022003992 A BR112022003992 A BR 112022003992A BR 112022003992 A BR112022003992 A BR 112022003992A BR 112022003992 A2 BR112022003992 A2 BR 112022003992A2
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BR
Brazil
Prior art keywords
nucleic acid
crm197 protein
signal sequence
expression vector
recombinant microorganism
Prior art date
Application number
BR112022003992A
Other languages
English (en)
Inventor
Chankyu Lee
Joong Kim Eui
Eun-Hye Park
Hyun-Do Kim
Gu Pan Jae
Hyun Kim Jeong
Hyun Kang Jung
Minchul Park
Original Assignee
Eubiologics Co Ltd
Genofocus Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Eubiologics Co Ltd, Genofocus Co Ltd filed Critical Eubiologics Co Ltd
Publication of BR112022003992A2 publication Critical patent/BR112022003992A2/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/34—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Corynebacterium (G)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K14/245—Escherichia (G)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
    • C07K7/04—Linear peptides containing only normal peptide links
    • C07K7/08—Linear peptides containing only normal peptide links having 12 to 20 amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1077—Pentosyltransferases (2.4.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/15—Corynebacterium
    • C12R2001/16—Corynebacterium diphtheriae
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/185—Escherichia
    • C12R2001/19—Escherichia coli

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

sequência sinal, ácido nucleico, construto de ácido nucleico, vetor de expressão, microrganismo recombinante e método para produzir uma proteína crm197. a presente invenção se refere a uma sequência de sinal para expressar uma proteína crm197 em escherichia coli e secretar a mesma no periplasma, e um uso da mesma, e mais especificamente, a: uma sequência de sinal para expressar uma proteína crm197; um ácido nucleico para codificar a sequência de sinal; um construto de ácido nucleico ou vetor de expressão compreendendo o ácido nucleico e um gene de proteína crm197; um microrganismo recombinante tendo o construto de ácido nucleico ou vetor de expressão introduzido nele; e um método de produção de proteína crm197 compreendendo uma etapa para cultivar o microrganismo recombinante. de acordo com a presente invenção, uma proteína crm197 com as mesmas propriedades físico-químicas/imunológicas que a proteína isolada da bactéria parental pode ser expressa mesmo em escherichia coli regular da qual um potencial redox não é ajustado, e uma proteína crm197 tendo alta eficiência de secreção periplasmática pode ser produzida mesmo sem alterar o ph de um meio de cultura para aumentar a secreção no periplasma e, portanto, a presente invenção é muito útil na produção de proteína crm197.
BR112022003992A 2019-09-03 2019-10-02 Sequência sinal, ácido nucleico, construto de ácido nucleico, vetor de expressão, microrganismo recombinante e método para produzir uma proteína crm197 BR112022003992A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR1020190108892A KR102099342B1 (ko) 2019-09-03 2019-09-03 Crm197 단백질 발현 방법
PCT/KR2019/012899 WO2021045292A1 (ko) 2019-09-03 2019-10-02 Crm197 단백질 발현 방법

Publications (1)

Publication Number Publication Date
BR112022003992A2 true BR112022003992A2 (pt) 2022-06-21

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ID=70291826

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112022003992A BR112022003992A2 (pt) 2019-09-03 2019-10-02 Sequência sinal, ácido nucleico, construto de ácido nucleico, vetor de expressão, microrganismo recombinante e método para produzir uma proteína crm197

Country Status (8)

Country Link
US (1) US20220281928A1 (pt)
EP (1) EP4026842A4 (pt)
JP (3) JP2022551809A (pt)
KR (1) KR102099342B1 (pt)
CN (1) CN114929727A (pt)
BR (1) BR112022003992A2 (pt)
CA (1) CA3149784A1 (pt)
WO (1) WO2021045292A1 (pt)

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Also Published As

Publication number Publication date
CN114929727A (zh) 2022-08-19
EP4026842A1 (en) 2022-07-13
KR102099342B1 (ko) 2020-04-10
JP2022551809A (ja) 2022-12-14
JP2023139192A (ja) 2023-10-03
CA3149784A1 (en) 2021-03-11
JP7507292B2 (ja) 2024-06-27
EP4026842A4 (en) 2023-12-27
JP2023139191A (ja) 2023-10-03
US20220281928A1 (en) 2022-09-08
WO2021045292A1 (ko) 2021-03-11

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BR112022003992A2 (pt) Sequência sinal, ácido nucleico, construto de ácido nucleico, vetor de expressão, microrganismo recombinante e método para produzir uma proteína crm197