BR112021025191A2 - Cepa de bacillus thuringiensis, método para preparar uma composição, método para a obtenção de cristais purificados da cepa b. thuringiensis, composição, uso da cepa de b. thuringiensis, e método para identificar a presença de uma cepa de b. thuringiensis - Google Patents

Cepa de bacillus thuringiensis, método para preparar uma composição, método para a obtenção de cristais purificados da cepa b. thuringiensis, composição, uso da cepa de b. thuringiensis, e método para identificar a presença de uma cepa de b. thuringiensis

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Publication number
BR112021025191A2
BR112021025191A2 BR112021025191A BR112021025191A BR112021025191A2 BR 112021025191 A2 BR112021025191 A2 BR 112021025191A2 BR 112021025191 A BR112021025191 A BR 112021025191A BR 112021025191 A BR112021025191 A BR 112021025191A BR 112021025191 A2 BR112021025191 A2 BR 112021025191A2
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BR
Brazil
Prior art keywords
strain
thuringiensis
composition
identify
preparing
Prior art date
Application number
BR112021025191A
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English (en)
Inventor
Caballero Sánchez Javier
Original Assignee
Bioinsectis S L
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bioinsectis S L filed Critical Bioinsectis S L
Publication of BR112021025191A2 publication Critical patent/BR112021025191A2/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/44—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
    • A01N37/46—N-acyl derivatives
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
    • A01N63/22—Bacillus
    • A01N63/23—B. thuringiensis
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/50—Isolated enzymes; Isolated proteins
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P7/00—Arthropodicides
    • A01P7/04—Insecticides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/07—Bacillus
    • C12R2001/075—Bacillus thuringiensis

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Virology (AREA)
  • Environmental Sciences (AREA)
  • Plant Pathology (AREA)
  • Pest Control & Pesticides (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Dentistry (AREA)
  • Agronomy & Crop Science (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Chemical & Material Sciences (AREA)
  • Insects & Arthropods (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Toxicology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

cepa de bacillus thuringiensis, método para preparar uma composição, método para a obtenção de cristais purificados da cepa b. thuringiensis, composição, uso da cepa de b. thuringiensis, e método para identificar a presença de uma cepa de b. thuringiensis. a invenção fornece uma cepa de b. thuringiensis {bt) que não produz beta-exotoxina e que exibe atividade inseticida contra spodoptera frugiperda, um inseto cujo controle é fraco ou ineficaz com os produtos à base de bt atualmente disponíveis no mercado. assim, composições baseadas nesta cepa podem ser utilizadas como inseticidas ou para preparação de inseticidas, sendo preferencialmente que a composição utilizada seja uma combinação de esporos e proteínas cristalinas da cepa. o genoma da cepa contém uma combinação de pelo menos (6) diferentes genes cry e pelo menos (3) diferentes genes vip que não foram descritos para outras cepas bt e que podem ser usados para identificá-los.
BR112021025191A 2019-06-14 2020-06-14 Cepa de bacillus thuringiensis, método para preparar uma composição, método para a obtenção de cristais purificados da cepa b. thuringiensis, composição, uso da cepa de b. thuringiensis, e método para identificar a presença de uma cepa de b. thuringiensis BR112021025191A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP19382497 2019-06-14
PCT/EP2020/066418 WO2020249811A1 (en) 2019-06-14 2020-06-14 Bacillus thuringiensis strain

Publications (1)

Publication Number Publication Date
BR112021025191A2 true BR112021025191A2 (pt) 2022-06-14

Family

ID=67003422

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112021025191A BR112021025191A2 (pt) 2019-06-14 2020-06-14 Cepa de bacillus thuringiensis, método para preparar uma composição, método para a obtenção de cristais purificados da cepa b. thuringiensis, composição, uso da cepa de b. thuringiensis, e método para identificar a presença de uma cepa de b. thuringiensis

Country Status (10)

Country Link
US (1) US20220243169A1 (pt)
EP (1) EP3982729A1 (pt)
JP (1) JP2022536185A (pt)
CN (1) CN114901800A (pt)
BR (1) BR112021025191A2 (pt)
CA (1) CA3143393A1 (pt)
EA (1) EA202290046A1 (pt)
MX (1) MX2021015475A (pt)
WO (1) WO2020249811A1 (pt)
ZA (1) ZA202200606B (pt)

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5045469A (en) 1988-10-27 1991-09-03 Mycogen Corporation Novel bacillus thuringiensis isolate denoted B. T. PS81F, active against lepidopteran pests, and a gene encoding a lepidopteran-active toxin
US5322687A (en) 1993-07-29 1994-06-21 Ecogen Inc. Bacillus thuringiensis cryet4 and cryet5 toxin genes and proteins toxic to lepidopteran insects
US5545817A (en) 1994-03-11 1996-08-13 Calgene, Inc. Enhanced expression in a plant plastid
US7449552B2 (en) 2006-04-14 2008-11-11 Pioneer Hi-Bred International, Inc. Bacillus thuringiensis cry gene and protein
US7772465B2 (en) 2007-06-26 2010-08-10 Pioneer Hi-Bred International, Inc. Bacillus thuringiensis gene with lepidopteran activity
WO2010027793A1 (en) 2008-08-25 2010-03-11 Dow Agrosciences Llc Nematode-resistant plants, and modified bacillus thuringiensis cry genes and proteins
US8084416B2 (en) 2008-12-23 2011-12-27 Athenix Corp. AXMI-150 delta-endotoxin gene and methods for its use
EP2794887A2 (en) 2011-03-30 2014-10-29 Universidad Nacional Autonoma De Mexico Mutant bacillus thuringiensis cry genes and methods of use
NZ629649A (en) 2012-03-09 2017-03-31 Vestaron Corp Toxic peptide production, peptide expression in plants and combinations of cysteine rich peptides
CN103333230B (zh) 2013-07-09 2016-02-17 福建农林大学 苏云金芽孢杆菌基因cry1Da3及其应用
US20180127771A1 (en) 2016-11-10 2018-05-10 Iowa State University Research Foundation, Inc. Insecticidal toxins for plant resistance to hemiptera

Also Published As

Publication number Publication date
EA202290046A1 (ru) 2022-03-16
US20220243169A1 (en) 2022-08-04
CA3143393A1 (en) 2020-12-17
ZA202200606B (en) 2023-11-29
WO2020249811A1 (en) 2020-12-17
MX2021015475A (es) 2022-02-11
EP3982729A1 (en) 2022-04-20
JP2022536185A (ja) 2022-08-12
CN114901800A (zh) 2022-08-12

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