BR112018076429A2 - método para rastrear um mutante dentro de uma população de organismos aplicando uma abordagem de agrupamento e divisão - Google Patents

método para rastrear um mutante dentro de uma população de organismos aplicando uma abordagem de agrupamento e divisão

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Publication number
BR112018076429A2
BR112018076429A2 BR112018076429A BR112018076429A BR112018076429A2 BR 112018076429 A2 BR112018076429 A2 BR 112018076429A2 BR 112018076429 A BR112018076429 A BR 112018076429A BR 112018076429 A BR112018076429 A BR 112018076429A BR 112018076429 A2 BR112018076429 A2 BR 112018076429A2
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Brazil
Prior art keywords
organisms
dpcr
genome
clustering
mutant
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BR112018076429A
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English (en)
Inventor
Striebeck Alexander
Skadhauge Birgitte
Cecilie Thomsen Hanne
Wohlfahrt Rasmussen Magnus
Carciofi Massimiliano
Olsen Ole
Knudsen Søren
Wendt Toni
Original Assignee
Carlsberg As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Application filed by Carlsberg As filed Critical Carlsberg As
Publication of BR112018076429A2 publication Critical patent/BR112018076429A2/pt

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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/01—Preparation of mutants without inserting foreign genetic material therein; Screening processes therefor
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/005—Methods for micropropagation; Vegetative plant propagation using cell or tissue culture techniques
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
    • A01H6/46—Gramineae or Poaceae, e.g. ryegrass, rice, wheat or maize
    • A01H6/4624—Hordeum vulgarus [barley]
    • C—CHEMISTRY; METALLURGY
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1024—In vivo mutagenesis using high mutation rate "mutator" host strains by inserting genetic material, e.g. encoding an error prone polymerase, disrupting a gene for mismatch repair
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
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    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/13—Plant traits
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B30/00—Methods of screening libraries
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/02—Libraries contained in or displayed by microorganisms, e.g. bacteria or animal cells; Libraries contained in or displayed by vectors, e.g. plasmids; Libraries containing only microorganisms or vectors

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Developmental Biology & Embryology (AREA)
  • Botany (AREA)
  • Environmental Sciences (AREA)
  • Physiology (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Cell Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

nas abordagens de melhoramento de plantas tradicionais, a mutagênese química pode ser utilizada para introduzir substituições de nucleotídeos aleatoriamente no genoma de uma planta, i.e., sem possibilidades de controlar os locais de alterações de nucleotídeos. por causa das complexidades do genoma, a probabilidade estatística é extremamente pequena quando se trata de encontrar uma substituição predeterminada de nucleotídeos. a presente invenção, no entanto, demonstra como um novo uso alternativo da reação em cadeia da polimerase digital (dpcr), de preferência, a gota dpcr (ddpcr), é desenvolvida para explorar a descoberta de substituições de nucleotídeos específicos em genes mutados. toda a plataforma compreende um método de rastrear com uma biblioteca de organismos mutagenizados, sistemas baseados em pcr digital e uma configuração para propagar e analisar organismos mutados identificados.
BR112018076429A 2016-07-01 2017-06-23 método para rastrear um mutante dentro de uma população de organismos aplicando uma abordagem de agrupamento e divisão BR112018076429A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DKPA201670485 2016-07-01
PCT/EP2017/065516 WO2018001884A1 (en) 2016-07-01 2017-06-23 Method to screen for a mutant within a population of organisms by applying a pooling and splitting approach

Publications (1)

Publication Number Publication Date
BR112018076429A2 true BR112018076429A2 (pt) 2019-10-01

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Country Status (25)

Country Link
US (2) US11884972B2 (pt)
EP (3) EP3693458A1 (pt)
JP (2) JP7208797B2 (pt)
CN (2) CN114959098A (pt)
AR (1) AR110103A1 (pt)
AU (3) AU2017288960B2 (pt)
BR (1) BR112018076429A2 (pt)
CA (1) CA3026016A1 (pt)
CL (1) CL2018003710A1 (pt)
CO (1) CO2019000828A2 (pt)
CR (1) CR20190040A (pt)
CU (1) CU20180158A7 (pt)
DK (1) DK3478832T3 (pt)
EA (1) EA201892809A1 (pt)
ES (1) ES2916221T3 (pt)
IL (1) IL263451B (pt)
MA (3) MA54568A (pt)
MX (2) MX2018016110A (pt)
PE (1) PE20190228A1 (pt)
PL (1) PL3478832T3 (pt)
PT (1) PT3478832T (pt)
SG (1) SG11201811021YA (pt)
UY (1) UY37312A (pt)
WO (1) WO2018001884A1 (pt)
ZA (3) ZA201808218B (pt)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MA51410A (fr) 2017-12-28 2021-04-07 Carlsberg As Plantes céréalières présentant des propriétés de paroi cellulaire améliorées
CN108913698B (zh) * 2018-07-25 2021-10-26 安徽农业大学 一种与小麦穗发芽抗性/感性相关的caps标记及其应用
CN110724741B (zh) * 2019-07-24 2020-05-19 艾普拜生物科技(苏州)有限公司 一种检测微小残留白血病相关融合基因的引物、探针及其试剂盒
EP3785529A1 (en) 2019-08-28 2021-03-03 Carlsberg A/S Barley plants with altered protein content in grains
BR112022005455A2 (pt) 2019-10-10 2022-06-21 Carlsberg As Métodos para preparar plantas mutantes
BR112022015008A2 (pt) 2020-03-02 2022-10-11 Carlsberg As Plantas de cevada com alto limite de atividade de dextrinase
EP4029935A1 (en) 2021-01-14 2022-07-20 Carlsberg A/S S. eubayanus strain and hybrids thereof
WO2023076908A1 (en) * 2021-10-25 2023-05-04 Gt Molecular, Inc. Off-target blocking sequences to improve target discrimination by polymerase chain reaction
AU2022422286A1 (en) 2021-12-22 2024-07-04 BASF Agricultural Solutions Seed US LLC Improved screening method for genome edited events
AR128756A1 (es) 2022-03-11 2024-06-12 Carlsberg As Modulación de niveles de proteínas
WO2024105148A1 (en) 2022-11-16 2024-05-23 University Of Copenhagen Plants with low seed glucosinolate content
WO2024133900A1 (en) 2022-12-22 2024-06-27 University Of Copenhagen Improved (-)-sparteine production
CN117965576B (zh) * 2024-02-01 2024-08-27 北京林业大学 一种王百合苷生物合成基因LlHCT1及其应用

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Also Published As

Publication number Publication date
JP2022101703A (ja) 2022-07-06
EP3693458A1 (en) 2020-08-12
CN109477087A (zh) 2019-03-15
MA45531A (fr) 2019-05-08
PT3478832T (pt) 2022-05-19
CL2018003710A1 (es) 2019-03-22
CN109477087B (zh) 2022-06-24
ES2916221T3 (es) 2022-06-29
SG11201811021YA (en) 2019-01-30
CO2019000828A2 (es) 2019-04-30
ZA201907775B (en) 2022-09-28
US20240182961A1 (en) 2024-06-06
CN114959098A (zh) 2022-08-30
PL3478832T3 (pl) 2022-06-06
AU2019268103A1 (en) 2019-12-12
UY37312A (es) 2018-02-28
CR20190040A (es) 2019-05-09
PE20190228A1 (es) 2019-02-13
AU2019268102A1 (en) 2019-12-12
IL263451A (en) 2019-01-31
MA54568A (fr) 2021-10-27
AU2019268102B2 (en) 2020-03-05
ZA201907774B (en) 2022-07-27
AU2017288960A1 (en) 2019-01-17
EP3478832A1 (en) 2019-05-08
US20190194723A1 (en) 2019-06-27
MA52594A (fr) 2021-03-17
EP3792357A1 (en) 2021-03-17
EA201892809A1 (ru) 2019-06-28
US11884972B2 (en) 2024-01-30
IL263451B (en) 2022-08-01
JP2019524083A (ja) 2019-09-05
JP7208797B2 (ja) 2023-01-19
CU20180158A7 (es) 2019-08-06
AU2019268103B2 (en) 2020-03-12
DK3478832T3 (da) 2022-05-30
ZA201808218B (en) 2020-02-26
CA3026016A1 (en) 2018-01-04
JP7539428B2 (ja) 2024-08-23
AU2017288960B2 (en) 2019-12-12
MX2018016110A (es) 2019-05-30
EP3478832B1 (en) 2022-02-23
WO2018001884A1 (en) 2018-01-04
AR110103A1 (es) 2019-02-27
MX2022014965A (es) 2023-01-11

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