BR112017023418A2 - desbloqueio ortogonal de nucleotídeos - Google Patents
desbloqueio ortogonal de nucleotídeosInfo
- Publication number
- BR112017023418A2 BR112017023418A2 BR112017023418-1A BR112017023418A BR112017023418A2 BR 112017023418 A2 BR112017023418 A2 BR 112017023418A2 BR 112017023418 A BR112017023418 A BR 112017023418A BR 112017023418 A2 BR112017023418 A2 BR 112017023418A2
- Authority
- BR
- Brazil
- Prior art keywords
- different
- site
- primer extension
- extension products
- unlocking
- Prior art date
Links
- 125000003729 nucleotide group Chemical group 0.000 title abstract 3
- 239000002773 nucleotide Substances 0.000 title 1
- 230000002441 reversible effect Effects 0.000 abstract 4
- 230000000903 blocking effect Effects 0.000 abstract 3
- 150000007523 nucleic acids Chemical class 0.000 abstract 2
- 102000039446 nucleic acids Human genes 0.000 abstract 2
- 108020004707 nucleic acids Proteins 0.000 abstract 2
- 238000000034 method Methods 0.000 abstract 1
- 239000000203 mixture Substances 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
- C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2523/00—Reactions characterised by treatment of reaction samples
- C12Q2523/10—Characterised by chemical treatment
- C12Q2523/107—Chemical cleaving agents
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2523/00—Reactions characterised by treatment of reaction samples
- C12Q2523/30—Characterised by physical treatment
- C12Q2523/313—Irradiation, e.g. UV irradiation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/10—Modifications characterised by
- C12Q2525/155—Modifications characterised by incorporating/generating a new priming site
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/10—Modifications characterised by
- C12Q2525/186—Modifications characterised by incorporating a non-extendable or blocking moiety
Abstract
a presente invenção refere-se a um processo incluindo as etapas de: (a) fornecer um conjunto de sítios, em que cada sítio compreende uma mistura de diferentes moldes de ácidos nucleicos; (b) estender os iniciadores hibridizados para os diferentes moldes de ácido nucleico em cada um dos sítios com diferentes análogos de nucleotídeo com diferentes porções de bloqueio reversíveis, respectivamente, desse modo produzindo produtos de extensão de iniciador diferentes em cada sítio; (c) detectar os produtos de extensão de iniciador diferentes para distinguir os diferentes análogos de nucleotídeo em cada sítio; e (d) remover as diferentes porções de bloqueio reversíveis dos produtos de extensão de iniciador em cada um dos sítios utilizando um primeiro tratamento que é seletivo para uma primeira das diferentes porções de bloqueio reversíveis e um segundo tratamento que é seletivo para uma segunda das diferentes porções de bloqueio reversíveis.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201562198947P | 2015-07-30 | 2015-07-30 | |
US62/198,947 | 2015-07-30 | ||
PCT/US2016/041568 WO2017019278A1 (en) | 2015-07-30 | 2016-07-08 | Orthogonal deblocking of nucleotides |
Publications (1)
Publication Number | Publication Date |
---|---|
BR112017023418A2 true BR112017023418A2 (pt) | 2018-07-24 |
Family
ID=56418645
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
BR112017023418-1A BR112017023418A2 (pt) | 2015-07-30 | 2016-07-08 | desbloqueio ortogonal de nucleotídeos |
Country Status (14)
Country | Link |
---|---|
US (2) | US11155864B2 (pt) |
EP (1) | EP3329007B1 (pt) |
JP (1) | JP6712606B2 (pt) |
KR (1) | KR102189965B1 (pt) |
CN (2) | CN107771223B (pt) |
AU (1) | AU2016298541B2 (pt) |
BR (1) | BR112017023418A2 (pt) |
CA (1) | CA2984702A1 (pt) |
DK (1) | DK3329007T3 (pt) |
ES (1) | ES2864677T3 (pt) |
HK (1) | HK1249148A1 (pt) |
IL (3) | IL294145B2 (pt) |
RU (1) | RU2742955C2 (pt) |
WO (1) | WO2017019278A1 (pt) |
Families Citing this family (7)
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AU2019276719A1 (en) * | 2018-05-31 | 2020-11-26 | Pacific Biosciences Of California, Inc. | Increased signal to noise in nucleic acid sequencing |
US20210332351A1 (en) * | 2018-07-23 | 2021-10-28 | Dna Script | Massively Parallel Enzymatic Synthesis of Nucleic Acid Strands |
CA3198842A1 (en) | 2020-10-21 | 2022-04-28 | Illumina, Inc. | Sequencing templates comprising multiple inserts and compositions and methods for improving sequencing throughput |
WO2022232308A1 (en) * | 2021-04-27 | 2022-11-03 | Singular Genomics Systems, Inc. | High density sequencing and multiplexed priming |
WO2023114392A1 (en) * | 2021-12-16 | 2023-06-22 | Ultima Genomics, Inc. | Systems and methods for sequencing with multi-priming |
WO2023154712A1 (en) * | 2022-02-08 | 2023-08-17 | Reticula, Inc. | Methods, compositions, and systems for long read single molecule sequencing |
WO2023175024A1 (en) * | 2022-03-15 | 2023-09-21 | Illumina, Inc. | Paired-end sequencing |
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2016
- 2016-07-08 US US15/739,587 patent/US11155864B2/en active Active
- 2016-07-08 EP EP16739665.4A patent/EP3329007B1/en active Active
- 2016-07-08 ES ES16739665T patent/ES2864677T3/es active Active
- 2016-07-08 JP JP2017556955A patent/JP6712606B2/ja active Active
- 2016-07-08 IL IL294145A patent/IL294145B2/en unknown
- 2016-07-08 CN CN201680036416.6A patent/CN107771223B/zh active Active
- 2016-07-08 RU RU2017137776A patent/RU2742955C2/ru active
- 2016-07-08 CN CN202210207353.3A patent/CN114634976A/zh active Pending
- 2016-07-08 WO PCT/US2016/041568 patent/WO2017019278A1/en active Application Filing
- 2016-07-08 KR KR1020177037553A patent/KR102189965B1/ko active IP Right Grant
- 2016-07-08 DK DK16739665.4T patent/DK3329007T3/da active
- 2016-07-08 CA CA2984702A patent/CA2984702A1/en active Pending
- 2016-07-08 BR BR112017023418-1A patent/BR112017023418A2/pt active Search and Examination
- 2016-07-08 IL IL255445A patent/IL255445B/en unknown
- 2016-07-08 AU AU2016298541A patent/AU2016298541B2/en active Active
- 2016-07-08 IL IL305561A patent/IL305561A/en unknown
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2018
- 2018-07-10 HK HK18108938.8A patent/HK1249148A1/zh unknown
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2021
- 2021-10-13 US US17/500,673 patent/US20220033898A1/en active Pending
Also Published As
Publication number | Publication date |
---|---|
RU2017137776A (ru) | 2019-08-29 |
CA2984702A1 (en) | 2017-02-02 |
IL294145B2 (en) | 2024-02-01 |
US11155864B2 (en) | 2021-10-26 |
KR20180014054A (ko) | 2018-02-07 |
US20180312917A1 (en) | 2018-11-01 |
IL255445A0 (en) | 2017-12-31 |
JP2018518947A (ja) | 2018-07-19 |
IL294145B1 (en) | 2023-10-01 |
WO2017019278A1 (en) | 2017-02-02 |
HK1249148A1 (zh) | 2018-10-26 |
EP3329007A1 (en) | 2018-06-06 |
CN114634976A (zh) | 2022-06-17 |
AU2016298541B2 (en) | 2019-10-31 |
CN107771223B (zh) | 2022-03-22 |
DK3329007T3 (da) | 2021-04-26 |
CN107771223A (zh) | 2018-03-06 |
IL255445B (en) | 2022-07-01 |
RU2742955C2 (ru) | 2021-02-12 |
KR102189965B1 (ko) | 2020-12-11 |
ES2864677T3 (es) | 2021-10-14 |
AU2016298541A1 (en) | 2017-11-16 |
RU2017137776A3 (pt) | 2019-08-29 |
IL305561A (en) | 2023-10-01 |
IL294145A (en) | 2022-08-01 |
JP6712606B2 (ja) | 2020-06-24 |
EP3329007B1 (en) | 2021-02-24 |
US20220033898A1 (en) | 2022-02-03 |
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