BR112017016935A2 - sistema e métodos para calibrar corantes de ligação - Google Patents

sistema e métodos para calibrar corantes de ligação

Info

Publication number
BR112017016935A2
BR112017016935A2 BR112017016935A BR112017016935A BR112017016935A2 BR 112017016935 A2 BR112017016935 A2 BR 112017016935A2 BR 112017016935 A BR112017016935 A BR 112017016935A BR 112017016935 A BR112017016935 A BR 112017016935A BR 112017016935 A2 BR112017016935 A2 BR 112017016935A2
Authority
BR
Brazil
Prior art keywords
dye
calibrating
channel
methods
sample holder
Prior art date
Application number
BR112017016935A
Other languages
English (en)
Other versions
BR112017016935B1 (pt
Inventor
Freudenthal Jacob
Marks Jeffrey
Chu Yong
Original Assignee
Life Technologies Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Life Technologies Corp filed Critical Life Technologies Corp
Publication of BR112017016935A2 publication Critical patent/BR112017016935A2/pt
Publication of BR112017016935B1 publication Critical patent/BR112017016935B1/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
    • G01N21/27—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands using photo-electric detection ; circuits for computing concentration
    • G01N21/274—Calibration, base line adjustment, drift correction
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6452—Individual samples arranged in a regular 2D-array, e.g. multiwell plates
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N2021/6417—Spectrofluorimetric devices
    • G01N2021/6421—Measuring at two or more wavelengths
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
    • G01N2021/6441—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks with two or more labels
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2201/00—Features of devices classified in G01N21/00
    • G01N2201/12—Circuits of general importance; Signal processing
    • G01N2201/127—Calibration; base line adjustment; drift compensation

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Optics & Photonics (AREA)
  • Mathematical Physics (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Theoretical Computer Science (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

é descrito um método implantado por computador (500) para calibrar um corante fluorescente. o método (500) compreende imagear um suporte de amostra, carregado em um instrumento, em mais de um canal. o suporte de amostra compreende uma pluralidade de locais de reação e mais de um tipo de corante, sendo que cada corante ocupa mais de um local de reação. o método compreende adicionalmente identificar um canal de pico para cada corante no suporte de amostra (508), normalizar cada canal para o canal de pico para cada corante (510) e produzir uma matriz de corante (518), que compreende um conjunto de valores de referência de corante.
BR112017016935-5A 2015-02-06 2016-02-05 método implementado por computador, mídia de armazenamento legível por computador não transitória e sistema para calibrar um corante fluorescente BR112017016935B1 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201562113118P 2015-02-06 2015-02-06
US62/113,118 2015-02-06
PCT/US2016/016817 WO2016127080A1 (en) 2015-02-06 2016-02-05 System and methods for calibrating binding dyes

Publications (2)

Publication Number Publication Date
BR112017016935A2 true BR112017016935A2 (pt) 2018-04-03
BR112017016935B1 BR112017016935B1 (pt) 2021-04-20

Family

ID=55453275

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112017016935-5A BR112017016935B1 (pt) 2015-02-06 2016-02-05 método implementado por computador, mídia de armazenamento legível por computador não transitória e sistema para calibrar um corante fluorescente

Country Status (9)

Country Link
US (1) US10501788B2 (pt)
EP (1) EP3254083B1 (pt)
JP (1) JP6789227B2 (pt)
KR (1) KR102370360B1 (pt)
CN (1) CN107407631B (pt)
BR (1) BR112017016935B1 (pt)
RU (1) RU2017131051A (pt)
SG (1) SG11201706352QA (pt)
WO (1) WO2016127080A1 (pt)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114414542B (zh) * 2021-12-20 2024-01-12 成都瀚辰光翼科技有限责任公司 用于基因检测的荧光信号检测方法、装置、介质及设备

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0814828A (ja) * 1994-06-29 1996-01-19 Kobe Steel Ltd ステレオ画像センサのキャリブレーション方法及びその装置
AU8846498A (en) * 1997-08-07 1999-03-01 Imaging Research, Inc. A digital imaging system for assays in well plates, gels and blots
CA2331678C (en) * 1998-05-19 2009-09-15 Cepheid Multi-channel optical detection system
US6905881B2 (en) * 2000-11-30 2005-06-14 Paul Sammak Microbead-based test plates and test methods for fluorescence imaging systems
US8084260B2 (en) * 2004-11-24 2011-12-27 Applied Biosystems, Llc Spectral calibration method and system for multiple instruments
US7663750B2 (en) * 2005-06-30 2010-02-16 Applied Biosystems, Llc Two-dimensional spectral imaging system
US7630849B2 (en) 2005-09-01 2009-12-08 Applied Biosystems, Llc Method of automated calibration and diagnosis of laboratory instruments
CN102621053B (zh) * 2005-09-21 2015-05-06 卢米尼克斯股份有限公司 图像数据处理的方法和系统
WO2008003053A2 (en) * 2006-06-28 2008-01-03 Applera Corporation Minimizing effects of dye crosstalk
US20080001099A1 (en) 2006-07-01 2008-01-03 Sharaf Muhammad A Quantitative calibration method and system for genetic analysis instrumentation
US8005628B2 (en) * 2007-01-29 2011-08-23 Applied Biosystems, Llc Systems and methods for baseline correction using non-linear normalization
CN102341710B (zh) * 2009-04-15 2015-04-01 比奥卡尔齐什股份有限公司 用于监视rtPCR反应的光学检测系统
JP5985140B2 (ja) * 2010-04-28 2016-09-06 ソニー株式会社 蛍光強度補正方法、蛍光強度算出方法及び蛍光強度算出装置
JP5081313B2 (ja) * 2011-04-06 2012-11-28 アイシン精機株式会社 車載カメラの校正装置
CN102411024B (zh) * 2011-06-15 2014-07-09 公安部第一研究所 一种基于空间校正及光谱校正的毛细管阵列电泳检测方法
JP6105061B2 (ja) * 2012-06-15 2017-03-29 ルミネックス コーポレーション ガウシアンフィット残差選択基準を用いて画像を正規化するための装置、システム及び方法

Also Published As

Publication number Publication date
WO2016127080A1 (en) 2016-08-11
CN107407631B (zh) 2020-11-03
EP3254083B1 (en) 2022-03-02
CN107407631A (zh) 2017-11-28
BR112017016935B1 (pt) 2021-04-20
US20160230209A1 (en) 2016-08-11
JP2018511786A (ja) 2018-04-26
US10501788B2 (en) 2019-12-10
EP3254083A1 (en) 2017-12-13
KR20170134358A (ko) 2017-12-06
KR102370360B1 (ko) 2022-03-04
RU2017131051A (ru) 2019-03-06
JP6789227B2 (ja) 2020-11-25
SG11201706352QA (en) 2017-09-28

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Legal Events

Date Code Title Description
B06U Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette]
B09A Decision: intention to grant [chapter 9.1 patent gazette]
B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]

Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 05/02/2016, OBSERVADAS AS CONDICOES LEGAIS.