BR112017000168A2 - caracterização fenotípica e genotipagem in-situ de uma biblioteca de células geneticamente diferentes - Google Patents

caracterização fenotípica e genotipagem in-situ de uma biblioteca de células geneticamente diferentes

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Publication number
BR112017000168A2
BR112017000168A2 BR112017000168-3A BR112017000168A BR112017000168A2 BR 112017000168 A2 BR112017000168 A2 BR 112017000168A2 BR 112017000168 A BR112017000168 A BR 112017000168A BR 112017000168 A2 BR112017000168 A2 BR 112017000168A2
Authority
BR
Brazil
Prior art keywords
culture device
different cell
cell library
genetically different
phenotypic characterization
Prior art date
Application number
BR112017000168-3A
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English (en)
Other versions
BR112017000168B1 (pt
Inventor
Elf Johan
Öhman Ove
Church George
Original Assignee
Elf Johan
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Elf Johan filed Critical Elf Johan
Publication of BR112017000168A2 publication Critical patent/BR112017000168A2/pt
Publication of BR112017000168B1 publication Critical patent/BR112017000168B1/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/18—Testing for antimicrobial activity of a material
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6841—In situ hybridisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/4833—Physical analysis of biological material of solid biological material, e.g. tissue samples, cell cultures

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Hematology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Food Science & Technology (AREA)
  • Optics & Photonics (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Plant Pathology (AREA)
  • Urology & Nephrology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Toxicology (AREA)
  • Fluid Mechanics (AREA)
  • Dispersion Chemistry (AREA)
  • Clinical Laboratory Science (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Optical Measuring Cells (AREA)

Abstract

uma biblioteca de linhagens de células é caracterizada por células nas posições espaciais definidas e separadas de cultura em um dispositivo de cultura (1) e a determinação de uma característica fenotípica de cada linhagem celular no dispositivo de cultura (1). as células são fixadas nas posições espacialmente definidos e separados no dispositivo de cultura (1), seguido pela genotipagem in situ de uma respectiva região variável (150) de cada linhagem celular nas posições espacialmente definidas e separadas no dispositivo de cultura (1). cada respectiva característica fenotípica é conectada para cada respectivo genótipo com base nas posições espacialmente definidas e separadas no dispositivo de cultura (1).
BR112017000168-3A 2014-07-07 2015-02-27 Método e sistema de caracterização de uma biblioteca de uma pluralidade de linhagens de células de diferentes genótipos e possuindo regiões variáveis diferentes BR112017000168B1 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
SE1450860-0 2014-07-07
SE1450860 2014-07-07
PCT/SE2015/050227 WO2016007063A1 (en) 2014-07-07 2015-02-27 Phenotypic characterization and in situ genotyping of a library of genetically different cells

Publications (2)

Publication Number Publication Date
BR112017000168A2 true BR112017000168A2 (pt) 2018-01-16
BR112017000168B1 BR112017000168B1 (pt) 2023-04-18

Family

ID=55064558

Family Applications (2)

Application Number Title Priority Date Filing Date
BR112017000168-3A BR112017000168B1 (pt) 2014-07-07 2015-02-27 Método e sistema de caracterização de uma biblioteca de uma pluralidade de linhagens de células de diferentes genótipos e possuindo regiões variáveis diferentes
BR112017000167-5A BR112017000167B1 (pt) 2014-07-07 2015-06-12 Dispositivo de microfluidos, método de carregamento de dispositivo de microfluidos, método para testes de sensibilidade aos antibióticos, método para genotipagem de células in situ e método para a caracterização do fenótipo de células

Family Applications After (1)

Application Number Title Priority Date Filing Date
BR112017000167-5A BR112017000167B1 (pt) 2014-07-07 2015-06-12 Dispositivo de microfluidos, método de carregamento de dispositivo de microfluidos, método para testes de sensibilidade aos antibióticos, método para genotipagem de células in situ e método para a caracterização do fenótipo de células

Country Status (11)

Country Link
US (3) US10570437B2 (pt)
EP (2) EP3167061B1 (pt)
JP (3) JP6600349B2 (pt)
CN (2) CN106471122B (pt)
AU (2) AU2015288389B2 (pt)
BR (2) BR112017000168B1 (pt)
CA (2) CA2954360C (pt)
DK (1) DK3167044T3 (pt)
ES (1) ES2762914T3 (pt)
PL (1) PL3167044T3 (pt)
WO (2) WO2016007063A1 (pt)

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US11527324B2 (en) 2017-11-10 2022-12-13 Reliant Immune Diagnostics, Inc. Artificial intelligence response system based on testing with parallel/serial dual microfluidic chip
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US11421273B2 (en) * 2018-12-03 2022-08-23 California Institute Of Technology In situ readout of DNA barcodes
CN109580485A (zh) * 2019-01-18 2019-04-05 大连理工大学 一种用于红细胞超分辨成像的固定装置和使用方法
WO2021062107A1 (en) * 2019-09-26 2021-04-01 Jumpcode Genomics, Inc. Method and system for targeted nucleic acid sequencing
WO2022197490A1 (en) * 2021-03-16 2022-09-22 Scale Biosciences Inc. Method for adding spatially-addressable barcodes to nucleic acids of a cellular sample in situ
CN116640643A (zh) 2022-02-24 2023-08-25 希森美康阿斯特雷戈公司 分析生物样品的卡盒和方法

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Also Published As

Publication number Publication date
ES2762914T3 (es) 2020-05-26
US10041104B2 (en) 2018-08-07
EP3167061A1 (en) 2017-05-17
US20180320216A1 (en) 2018-11-08
US10570437B2 (en) 2020-02-25
CN106471122A (zh) 2017-03-01
DK3167044T3 (da) 2019-12-02
AU2015288329A1 (en) 2017-01-12
EP3167044A4 (en) 2018-06-20
JP2017519517A (ja) 2017-07-20
AU2015288329B2 (en) 2020-07-23
WO2016007068A1 (en) 2016-01-14
US20170137861A1 (en) 2017-05-18
AU2015288389B2 (en) 2021-05-13
BR112017000168B1 (pt) 2023-04-18
CN106661530A (zh) 2017-05-10
BR112017000167A2 (pt) 2018-01-16
EP3167044A1 (en) 2017-05-17
EP3167061A4 (en) 2018-02-28
PL3167044T3 (pl) 2020-08-24
JP6600349B2 (ja) 2019-10-30
EP3167061B1 (en) 2019-02-20
CN106661530B (zh) 2021-06-04
US20170159048A1 (en) 2017-06-08
CA2954360A1 (en) 2016-01-14
US10913969B2 (en) 2021-02-09
CN106471122B (zh) 2020-07-03
CA2954378A1 (en) 2016-01-14
JP6629287B2 (ja) 2020-01-15
BR112017000167B1 (pt) 2021-08-24
CA2954378C (en) 2022-11-22
JP2020054365A (ja) 2020-04-09
JP2017520262A (ja) 2017-07-27
EP3167044B1 (en) 2019-10-09
AU2015288389A1 (en) 2017-01-12
JP7066206B2 (ja) 2022-05-13
CA2954360C (en) 2022-11-22
WO2016007063A1 (en) 2016-01-14

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Owner name: ASTREGO DIAGNOSTICS AB (SE)

B06U Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette]
B09A Decision: intention to grant [chapter 9.1 patent gazette]
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Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 27/02/2015, OBSERVADAS AS CONDICOES LEGAIS