BR112017000168A2 - caracterização fenotípica e genotipagem in-situ de uma biblioteca de células geneticamente diferentes - Google Patents
caracterização fenotípica e genotipagem in-situ de uma biblioteca de células geneticamente diferentesInfo
- Publication number
- BR112017000168A2 BR112017000168A2 BR112017000168-3A BR112017000168A BR112017000168A2 BR 112017000168 A2 BR112017000168 A2 BR 112017000168A2 BR 112017000168 A BR112017000168 A BR 112017000168A BR 112017000168 A2 BR112017000168 A2 BR 112017000168A2
- Authority
- BR
- Brazil
- Prior art keywords
- culture device
- different cell
- cell library
- genetically different
- phenotypic characterization
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/18—Testing for antimicrobial activity of a material
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6841—In situ hybridisation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
- C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/483—Physical analysis of biological material
- G01N33/4833—Physical analysis of biological material of solid biological material, e.g. tissue samples, cell cultures
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Physics & Mathematics (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Analytical Chemistry (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Hematology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Bioinformatics & Computational Biology (AREA)
- Food Science & Technology (AREA)
- Optics & Photonics (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Plant Pathology (AREA)
- Urology & Nephrology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Toxicology (AREA)
- Fluid Mechanics (AREA)
- Dispersion Chemistry (AREA)
- Clinical Laboratory Science (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Optical Measuring Cells (AREA)
Abstract
uma biblioteca de linhagens de células é caracterizada por células nas posições espaciais definidas e separadas de cultura em um dispositivo de cultura (1) e a determinação de uma característica fenotípica de cada linhagem celular no dispositivo de cultura (1). as células são fixadas nas posições espacialmente definidos e separados no dispositivo de cultura (1), seguido pela genotipagem in situ de uma respectiva região variável (150) de cada linhagem celular nas posições espacialmente definidas e separadas no dispositivo de cultura (1). cada respectiva característica fenotípica é conectada para cada respectivo genótipo com base nas posições espacialmente definidas e separadas no dispositivo de cultura (1).
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
SE1450860-0 | 2014-07-07 | ||
SE1450860 | 2014-07-07 | ||
PCT/SE2015/050227 WO2016007063A1 (en) | 2014-07-07 | 2015-02-27 | Phenotypic characterization and in situ genotyping of a library of genetically different cells |
Publications (2)
Publication Number | Publication Date |
---|---|
BR112017000168A2 true BR112017000168A2 (pt) | 2018-01-16 |
BR112017000168B1 BR112017000168B1 (pt) | 2023-04-18 |
Family
ID=55064558
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
BR112017000168-3A BR112017000168B1 (pt) | 2014-07-07 | 2015-02-27 | Método e sistema de caracterização de uma biblioteca de uma pluralidade de linhagens de células de diferentes genótipos e possuindo regiões variáveis diferentes |
BR112017000167-5A BR112017000167B1 (pt) | 2014-07-07 | 2015-06-12 | Dispositivo de microfluidos, método de carregamento de dispositivo de microfluidos, método para testes de sensibilidade aos antibióticos, método para genotipagem de células in situ e método para a caracterização do fenótipo de células |
Family Applications After (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
BR112017000167-5A BR112017000167B1 (pt) | 2014-07-07 | 2015-06-12 | Dispositivo de microfluidos, método de carregamento de dispositivo de microfluidos, método para testes de sensibilidade aos antibióticos, método para genotipagem de células in situ e método para a caracterização do fenótipo de células |
Country Status (11)
Country | Link |
---|---|
US (3) | US10570437B2 (pt) |
EP (2) | EP3167061B1 (pt) |
JP (3) | JP6600349B2 (pt) |
CN (2) | CN106471122B (pt) |
AU (2) | AU2015288389B2 (pt) |
BR (2) | BR112017000168B1 (pt) |
CA (2) | CA2954360C (pt) |
DK (1) | DK3167044T3 (pt) |
ES (1) | ES2762914T3 (pt) |
PL (1) | PL3167044T3 (pt) |
WO (2) | WO2016007063A1 (pt) |
Families Citing this family (21)
Publication number | Priority date | Publication date | Assignee | Title |
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CA2954360C (en) * | 2014-07-07 | 2022-11-22 | Johan ELF | Phenotypic characterization and in situ genotyping of a library of genetically different cells |
WO2016149422A1 (en) | 2015-03-16 | 2016-09-22 | The Broad Institute, Inc. | Encoding of dna vector identity via iterative hybridization detection of a barcode transcript |
BR112017023188B1 (pt) * | 2015-05-11 | 2023-11-14 | Illumina, Inc | Plataforma para descoberta e análise de agentes terapêuticos |
EP4050112A1 (en) | 2016-06-21 | 2022-08-31 | 10X Genomics, Inc. | Nucleic acid sequencing |
US11976316B2 (en) | 2017-03-22 | 2024-05-07 | Astrego Diagnostics Ab | Phenotypic characterization of cells |
JP7214244B2 (ja) * | 2017-10-31 | 2023-01-30 | アストレゴ ダイアグノスティクス エービー | 細胞の特徴付けを行うためのマイクロ流体デバイス |
US11527324B2 (en) | 2017-11-10 | 2022-12-13 | Reliant Immune Diagnostics, Inc. | Artificial intelligence response system based on testing with parallel/serial dual microfluidic chip |
US11041185B2 (en) | 2017-11-10 | 2021-06-22 | Reliant Immune Diagnostics, Inc. | Modular parallel/serial dual microfluidic chip |
US11200986B2 (en) | 2017-11-10 | 2021-12-14 | Reliant Immune Diagnostics, Inc. | Database and machine learning in response to parallel serial dual microfluidic chip |
US11124821B2 (en) | 2017-11-10 | 2021-09-21 | Reliant Immune Diagnostics, Inc. | Microfluidic testing system with cell capture/analysis regions for processing in a parallel and serial manner |
US10930381B2 (en) | 2017-11-10 | 2021-02-23 | Reliant Immune Diagnostics, Inc. | Microfluidic testing system for mobile veterinary applications |
US10930380B2 (en) | 2017-11-10 | 2021-02-23 | Reliant Immune Diagnostics, Inc. | Communication loop and record loop system for parallel/serial dual microfluidic chip |
EP3752829A4 (en) * | 2018-02-16 | 2021-11-03 | Astrego Diagnostics AB | CAPTURE OF CELLS IN MICROFLUIDIC DEVICES |
JP7341148B2 (ja) | 2018-02-16 | 2023-09-08 | アストレゴ ダイアグノスティクス エービー | 微小流体装置 |
JP7282327B2 (ja) * | 2018-08-23 | 2023-05-29 | 株式会社デンソー | 細胞固定デバイス、細胞固定方法、及び細胞操作方法 |
SE542462C2 (en) | 2018-09-20 | 2020-05-12 | Astrego Diagnostics Ab | Sample loading cartridge for a microfluidic device |
US11421273B2 (en) * | 2018-12-03 | 2022-08-23 | California Institute Of Technology | In situ readout of DNA barcodes |
CN109580485A (zh) * | 2019-01-18 | 2019-04-05 | 大连理工大学 | 一种用于红细胞超分辨成像的固定装置和使用方法 |
WO2021062107A1 (en) * | 2019-09-26 | 2021-04-01 | Jumpcode Genomics, Inc. | Method and system for targeted nucleic acid sequencing |
WO2022197490A1 (en) * | 2021-03-16 | 2022-09-22 | Scale Biosciences Inc. | Method for adding spatially-addressable barcodes to nucleic acids of a cellular sample in situ |
CN116640643A (zh) | 2022-02-24 | 2023-08-25 | 希森美康阿斯特雷戈公司 | 分析生物样品的卡盒和方法 |
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AU2003224817B2 (en) | 2002-04-01 | 2008-11-06 | Fluidigm Corporation | Microfluidic particle-analysis systems |
JP4895504B2 (ja) * | 2002-04-30 | 2012-03-14 | ユィロス・パテント・アクチボラグ | 集中マイクロ流体デバイス(ea) |
EP1525451B1 (en) | 2002-04-30 | 2009-07-15 | Gyros Patent Ab | Method of analysing a catalytic system using an integrated microfluidic device |
CN101165161B (zh) * | 2007-07-27 | 2013-10-30 | 中国科学院上海微系统与信息技术研究所 | 一种微流体浓度梯度细胞培养芯片及其制备方法和应用 |
CN101842159B (zh) * | 2007-08-09 | 2014-09-24 | 赛路拉公司 | 关联的多参数单细胞测定及残余生物材料回收的方法和装置 |
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SG11201504558TA (en) | 2012-12-11 | 2015-07-30 | Einstein Coll Med | Methods for high throughput receptor:ligand identification |
WO2014152048A2 (en) * | 2013-03-14 | 2014-09-25 | Cytonome/St, Llc | Assemblies and methods for reducing optical crosstalk in particle processing systems |
CA2954360C (en) * | 2014-07-07 | 2022-11-22 | Johan ELF | Phenotypic characterization and in situ genotyping of a library of genetically different cells |
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2015
- 2015-02-27 CA CA2954360A patent/CA2954360C/en active Active
- 2015-02-27 CN CN201580036764.9A patent/CN106471122B/zh active Active
- 2015-02-27 US US15/323,352 patent/US10570437B2/en active Active
- 2015-02-27 AU AU2015288389A patent/AU2015288389B2/en active Active
- 2015-02-27 WO PCT/SE2015/050227 patent/WO2016007063A1/en active Application Filing
- 2015-02-27 JP JP2017501328A patent/JP6600349B2/ja active Active
- 2015-02-27 EP EP15818135.4A patent/EP3167061B1/en active Active
- 2015-02-27 BR BR112017000168-3A patent/BR112017000168B1/pt active IP Right Grant
- 2015-06-12 BR BR112017000167-5A patent/BR112017000167B1/pt active IP Right Grant
- 2015-06-12 PL PL15818165T patent/PL3167044T3/pl unknown
- 2015-06-12 WO PCT/SE2015/050685 patent/WO2016007068A1/en active Application Filing
- 2015-06-12 DK DK15818165T patent/DK3167044T3/da active
- 2015-06-12 EP EP15818165.1A patent/EP3167044B1/en active Active
- 2015-06-12 CN CN201580036437.3A patent/CN106661530B/zh active Active
- 2015-06-12 CA CA2954378A patent/CA2954378C/en active Active
- 2015-06-12 ES ES15818165T patent/ES2762914T3/es active Active
- 2015-06-12 JP JP2017501162A patent/JP6629287B2/ja active Active
- 2015-06-12 AU AU2015288329A patent/AU2015288329B2/en active Active
- 2015-06-12 US US15/323,357 patent/US10041104B2/en active Active
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2018
- 2018-07-19 US US16/039,541 patent/US10913969B2/en active Active
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2019
- 2019-12-04 JP JP2019219794A patent/JP7066206B2/ja active Active
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Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 27/02/2015, OBSERVADAS AS CONDICOES LEGAIS |