BR112016019081A8 - cepa recombinante produtora de o-aminobenzoato e produção fermentativa de anilina a partir de recursos renováveis via ácido 2-aminobenzoico - Google Patents

cepa recombinante produtora de o-aminobenzoato e produção fermentativa de anilina a partir de recursos renováveis via ácido 2-aminobenzoico

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Publication number
BR112016019081A8
BR112016019081A8 BR112016019081A BR112016019081A BR112016019081A8 BR 112016019081 A8 BR112016019081 A8 BR 112016019081A8 BR 112016019081 A BR112016019081 A BR 112016019081A BR 112016019081 A BR112016019081 A BR 112016019081A BR 112016019081 A8 BR112016019081 A8 BR 112016019081A8
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BR
Brazil
Prior art keywords
aminobenzoate
aniline
converting
renewable resources
aminobenzoic acid
Prior art date
Application number
BR112016019081A
Other languages
English (en)
Other versions
BR112016019081A2 (pt
BR112016019081B1 (pt
Inventor
Gernot Jaeger
Jorgen Magnus
Amgad Salah Moussa
Günter Olf
Giulio Lolli
Swantje Behnken
Jung-Won Youn
Mohammad Yalfani
Georg Sprenger
Original Assignee
Covestro Deutschland Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Covestro Deutschland Ag filed Critical Covestro Deutschland Ag
Publication of BR112016019081A2 publication Critical patent/BR112016019081A2/pt
Publication of BR112016019081A8 publication Critical patent/BR112016019081A8/pt
Publication of BR112016019081B1 publication Critical patent/BR112016019081B1/pt

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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/77—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Corynebacterium; for Brevibacterium
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1077—Pentosyltransferases (2.4.2)
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/02—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising inorganic material
    • B01J20/10—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising inorganic material comprising silica or silicate
    • B01J20/16—Alumino-silicates
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    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C209/00—Preparation of compounds containing amino groups bound to a carbon skeleton
    • C07C209/68—Preparation of compounds containing amino groups bound to a carbon skeleton from amines, by reactions not involving amino groups, e.g. reduction of unsaturated amines, aromatisation, or substitution of the carbon skeleton
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C211/00—Compounds containing amino groups bound to a carbon skeleton
    • C07C211/43—Compounds containing amino groups bound to a carbon skeleton having amino groups bound to carbon atoms of six-membered aromatic rings of the carbon skeleton
    • C07C211/44—Compounds containing amino groups bound to a carbon skeleton having amino groups bound to carbon atoms of six-membered aromatic rings of the carbon skeleton having amino groups bound to only one six-membered aromatic ring
    • C07C211/45—Monoamines
    • C07C211/46—Aniline
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    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/78—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Pseudomonas
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    • C12N9/90—Isomerases (5.)
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    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/001—Amines; Imines
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    • C12P13/002—Nitriles (-CN)
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    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/02—Amides, e.g. chloramphenicol or polyamides; Imides or polyimides; Urethanes, i.e. compounds comprising N-C=O structural element or polyurethanes
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    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
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    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/02—Pentosyltransferases (2.4.2)
    • C12Y204/02018—Anthranilate phosphoribosyltransferase (2.4.2.18)
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    • C12Y—ENZYMES
    • C12Y504/00—Intramolecular transferases (5.4)
    • C12Y504/99—Intramolecular transferases (5.4) transferring other groups (5.4.99)
    • C12Y504/99005—Chorismate mutase (5.4.99.5)

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Inorganic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

cepa recombinante produtora de o-aminobenzoato e produção fermentativa de anilina a partir de recursos renováveis via ácido 2-aminobenzoico a invenção proporciona uma célula hospedeira microbiana recombinante capaz de converter, biologicamente, uma matéria-prima compreendendo um substrato de carbono fermentável em o-aminobenzoato (oab). a invenção proporciona ainda um método para a produção de anilina, compreendendo as etapas de: a) produção de o-aminobenzoato por fermentação de uma matéria-prima compreendendo, pelo menos, um substrato de carbono fermentável utilizando a célula hospedeira microbiana recombinante, capaz de converter biologicamente a dita matéria-prima compreendendo, pelo menos, um substrato de carbono fermentável em o-aminobenzoato, em que o dito o-aminobenzoato compreende o ânion antranilato, b) conversão do dito o-aminobenzoato a partir do dito ânion antranilato em ácido antranílico por protonação ácida, c) recuperação do dito ácido antranílico por precipitação ou por dissolução em um solvente orgânico e d) conversão do dito ácido antranílico em anilina por descarboxilação térmica em um solvente orgânico.
BR112016019081-5A 2014-02-20 2015-02-19 Célula hospedeira microbiana recombinante e método para a produção de anilina BR112016019081B1 (pt)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
EP14155936.9 2014-02-20
EP14155936 2014-02-20
EP14196432.0 2014-12-05
EP14196432 2014-12-05
PCT/EP2015/053526 WO2015124687A1 (en) 2014-02-20 2015-02-19 Recombinant strain producing o-aminobenzoate and fermentative production of aniline from renewable resources via 2-aminobenzoic acid

Publications (3)

Publication Number Publication Date
BR112016019081A2 BR112016019081A2 (pt) 2017-08-15
BR112016019081A8 true BR112016019081A8 (pt) 2021-07-13
BR112016019081B1 BR112016019081B1 (pt) 2023-05-30

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Also Published As

Publication number Publication date
BR112016019081A2 (pt) 2017-08-15
US10731187B2 (en) 2020-08-04
TW201546275A (zh) 2015-12-16
CA2939194A1 (en) 2015-08-27
WO2015124687A1 (en) 2015-08-27
KR20160123351A (ko) 2016-10-25
US20170152535A1 (en) 2017-06-01
CN106414714A (zh) 2017-02-15
KR102335689B1 (ko) 2021-12-07
EP3108015A1 (en) 2016-12-28

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B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B06U Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette]
B350 Update of information on the portal [chapter 15.35 patent gazette]
B350 Update of information on the portal [chapter 15.35 patent gazette]
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B09A Decision: intention to grant [chapter 9.1 patent gazette]
B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]

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