BR112014016112B8 - Método e aparelho para caracterizar um polinucleotídeo alvo, kit, e, uso de uma helicase - Google Patents
Método e aparelho para caracterizar um polinucleotídeo alvo, kit, e, uso de uma helicaseInfo
- Publication number
- BR112014016112B8 BR112014016112B8 BR112014016112A BR112014016112A BR112014016112B8 BR 112014016112 B8 BR112014016112 B8 BR 112014016112B8 BR 112014016112 A BR112014016112 A BR 112014016112A BR 112014016112 A BR112014016112 A BR 112014016112A BR 112014016112 B8 BR112014016112 B8 BR 112014016112B8
- Authority
- BR
- Brazil
- Prior art keywords
- helicase
- target polynucleotide
- kit
- characterize
- pore
- Prior art date
Links
- 108060004795 Methyltransferase Proteins 0.000 title abstract 4
- 102000040430 polynucleotide Human genes 0.000 title abstract 4
- 108091033319 polynucleotide Proteins 0.000 title abstract 4
- 239000002157 polynucleotide Substances 0.000 title abstract 4
- 239000011148 porous material Substances 0.000 abstract 2
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y306/00—Hydrolases acting on acid anhydrides (3.6)
- C12Y306/04—Hydrolases acting on acid anhydrides (3.6) acting on acid anhydrides; involved in cellular and subcellular movement (3.6.4)
- C12Y306/04012—DNA helicase (3.6.4.12)
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Immunology (AREA)
- Analytical Chemistry (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Enzymes And Modification Thereof (AREA)
- Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201161581332P | 2011-12-29 | 2011-12-29 | |
| US61/581332 | 2011-12-29 | ||
| PCT/GB2012/053274 WO2013098562A2 (en) | 2011-12-29 | 2012-12-28 | Enzyme method |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| BR112014016112A2 BR112014016112A2 (pt) | 2020-07-14 |
| BR112014016112B1 BR112014016112B1 (pt) | 2022-05-24 |
| BR112014016112B8 true BR112014016112B8 (pt) | 2022-12-27 |
Family
ID=47561658
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BR112014016112A BR112014016112B8 (pt) | 2011-12-29 | 2012-12-28 | Método e aparelho para caracterizar um polinucleotídeo alvo, kit, e, uso de uma helicase |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US10385382B2 (https=) |
| EP (1) | EP2798084B1 (https=) |
| JP (1) | JP6228128B2 (https=) |
| KR (1) | KR102086182B1 (https=) |
| CN (1) | CN104126018B (https=) |
| AU (1) | AU2012360244B2 (https=) |
| BR (1) | BR112014016112B8 (https=) |
| CA (1) | CA2861808C (https=) |
| WO (1) | WO2013098562A2 (https=) |
Families Citing this family (132)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20110121840A1 (en) | 2007-02-20 | 2011-05-26 | Gurdial Singh Sanghera | Lipid Bilayer Sensor System |
| GB0724736D0 (en) | 2007-12-19 | 2008-01-30 | Oxford Nanolabs Ltd | Formation of layers of amphiphilic molecules |
| CA2774710C (en) | 2008-09-22 | 2016-08-02 | University Of Washington | Msp nanopores and related methods |
| EP2391732B1 (en) | 2009-01-30 | 2015-05-27 | Oxford Nanopore Technologies Limited | Methods using adaptors for nucleic acid constructs in transmembrane sequencing |
| EP4737389A2 (en) | 2011-05-27 | 2026-05-06 | Oxford Nanopore Technologies PLC | Coupling method |
| JP6298404B2 (ja) | 2011-07-25 | 2018-03-20 | オックスフォード ナノポール テクノロジーズ リミテッド | 膜貫通ポアを用いる二重鎖ポリヌクレオチド配列決定のためのヘアピンループ方法 |
| JP6457811B2 (ja) | 2011-09-23 | 2019-01-23 | オックスフォード ナノポール テクノロジーズ リミテッド | ポリマー単位を含むポリマーの解析 |
| CN104039979B (zh) | 2011-10-21 | 2016-08-24 | 牛津纳米孔技术公司 | 使用孔和Hel308解旋酶表征目标多核苷酸的酶方法 |
| US9617591B2 (en) | 2011-12-29 | 2017-04-11 | Oxford Nanopore Technologies Ltd. | Method for characterising a polynucleotide by using a XPD helicase |
| AU2012360244B2 (en) | 2011-12-29 | 2018-08-23 | Oxford Nanopore Technologies Limited | Enzyme method |
| GB201202519D0 (en) | 2012-02-13 | 2012-03-28 | Oxford Nanopore Tech Ltd | Apparatus for supporting an array of layers of amphiphilic molecules and method of forming an array of layers of amphiphilic molecules |
| IN2014DN06796A (https=) | 2012-02-15 | 2015-05-22 | Oxford Nanopore Tech Ltd | |
| CN104321441B (zh) | 2012-02-16 | 2016-10-19 | 牛津楠路珀尔科技有限公司 | 聚合物的测量的分析 |
| KR102083695B1 (ko) | 2012-04-10 | 2020-03-02 | 옥스포드 나노포어 테크놀로지즈 리미티드 | 돌연변이체 리세닌 기공 |
| US11155860B2 (en) | 2012-07-19 | 2021-10-26 | Oxford Nanopore Technologies Ltd. | SSB method |
| CA2879261C (en) | 2012-07-19 | 2022-12-06 | Oxford Nanopore Technologies Limited | Modified helicases |
| JP6429773B2 (ja) | 2012-07-19 | 2018-11-28 | オックスフォード ナノポール テクノロジーズ リミテッド | 酵素構築物 |
| US9551023B2 (en) | 2012-09-14 | 2017-01-24 | Oxford Nanopore Technologies Ltd. | Sample preparation method |
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| CA2901545C (en) | 2013-03-08 | 2019-10-08 | Oxford Nanopore Technologies Limited | Use of spacer elements in a nucleic acid to control movement of a helicase |
| GB201318465D0 (en) | 2013-10-18 | 2013-12-04 | Oxford Nanopore Tech Ltd | Method |
| GB201314695D0 (en) | 2013-08-16 | 2013-10-02 | Oxford Nanopore Tech Ltd | Method |
| GB201313477D0 (en) | 2013-07-29 | 2013-09-11 | Univ Leuven Kath | Nanopore biosensors for detection of proteins and nucleic acids |
| CN103265958A (zh) * | 2013-06-08 | 2013-08-28 | 东莞市保得生物工程有限公司 | 一种商品化有机物料腐熟剂的制备方法 |
| GB201406151D0 (en) | 2014-04-04 | 2014-05-21 | Oxford Nanopore Tech Ltd | Method |
| CN117947149A (zh) | 2013-10-18 | 2024-04-30 | 牛津纳米孔科技公开有限公司 | 经修饰的酶 |
| US9580758B2 (en) | 2013-11-12 | 2017-02-28 | Luc Montagnier | System and method for the detection and treatment of infection by a microbial agent associated with HIV infection |
| SI3074534T1 (sl) | 2013-11-26 | 2019-08-30 | Illumina, Inc. | Postopki določanja zaporedja polinukleotidov |
| CN111534504B (zh) | 2014-01-22 | 2024-06-21 | 牛津纳米孔科技公开有限公司 | 将一个或多个多核苷酸结合蛋白连接到靶多核苷酸的方法 |
| GB201406155D0 (en) | 2014-04-04 | 2014-05-21 | Oxford Nanopore Tech Ltd | Method |
| US10359395B2 (en) * | 2014-02-19 | 2019-07-23 | University Of Washington | Nanopore-based analysis of protein characteristics |
| GB201403096D0 (en) | 2014-02-21 | 2014-04-09 | Oxford Nanopore Tech Ltd | Sample preparation method |
| WO2015150786A1 (en) | 2014-04-04 | 2015-10-08 | Oxford Nanopore Technologies Limited | Method for characterising a double stranded nucleic acid using a nano-pore and anchor molecules at both ends of said nucleic acid |
| GB201417712D0 (en) | 2014-10-07 | 2014-11-19 | Oxford Nanopore Tech Ltd | Method |
| EP3137490B1 (en) | 2014-05-02 | 2021-01-27 | Oxford Nanopore Technologies Limited | Mutant pores |
| GB201411285D0 (en) | 2014-06-25 | 2014-08-06 | Prosser Joseph | Sequencer |
| CA2959220A1 (en) | 2014-09-01 | 2016-03-10 | Vib Vzw | Mutant csgg pores |
| EP3204511B1 (en) | 2014-10-07 | 2021-07-28 | Oxford Nanopore Technologies Limited | Mutant pores |
| GB201418159D0 (en) | 2014-10-14 | 2014-11-26 | Oxford Nanopore Tech Ltd | Method |
| EP4397972A3 (en) | 2014-10-16 | 2024-10-09 | Oxford Nanopore Technologies PLC | Alignment mapping estimation |
| US10480026B2 (en) | 2014-10-17 | 2019-11-19 | Oxford Nanopore Technologies Ltd. | Method for nanopore RNA characterisation |
| GB201418469D0 (en) | 2014-10-17 | 2014-12-03 | Oxford Nanopore Tech Ltd | Method |
| GB201418512D0 (en) | 2014-10-17 | 2014-12-03 | Oxford Nanopore Tech Ltd | Electrical device with detachable components |
| GB201502809D0 (en) | 2015-02-19 | 2015-04-08 | Oxford Nanopore Tech Ltd | Mutant pore |
| GB201502810D0 (en) | 2015-02-19 | 2015-04-08 | Oxford Nanopore Tech Ltd | Method |
| WO2016166232A1 (en) | 2015-04-14 | 2016-10-20 | Katholieke Universiteit Leuven | Nanopores with internal protein adaptors |
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| GB201508669D0 (en) | 2015-05-20 | 2015-07-01 | Oxford Nanopore Tech Ltd | Methods and apparatus for forming apertures in a solid state membrane using dielectric breakdown |
| JP6889701B2 (ja) * | 2015-09-24 | 2021-06-18 | エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ | アルファ溶血素バリアント |
| AU2016369071B2 (en) | 2015-12-08 | 2022-05-19 | Katholieke Universiteit Leuven Ku Leuven Research & Development | Modified nanopores, compositions comprising the same, and uses thereof |
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| GB201616590D0 (en) | 2016-09-29 | 2016-11-16 | Oxford Nanopore Technologies Limited | Method |
| GB201619930D0 (en) | 2016-11-24 | 2017-01-11 | Oxford Nanopore Tech | Apparatus and methods for controlling insertion of a membrane channel into a membrane |
| GB201620450D0 (en) | 2016-12-01 | 2017-01-18 | Oxford Nanopore Tech Ltd | Method |
| CA3053086A1 (en) | 2017-02-10 | 2018-08-16 | Oxford Nanopore Technologies Limited | Modified nanopores, compositions comprising the same, and uses thereof |
| GB201707122D0 (en) | 2017-05-04 | 2017-06-21 | Oxford Nanopore Tech Ltd | Pore |
| GB201707140D0 (en) | 2017-05-04 | 2017-06-21 | Oxford Nanopore Tech Ltd | Method |
| AU2018290975B2 (en) * | 2017-06-29 | 2024-10-03 | President And Fellows Of Harvard College | Deterministic stepping of polymers through a nanopore |
| CN117106038B (zh) | 2017-06-30 | 2025-12-09 | 弗拉芒区生物技术研究所 | 新颖蛋白孔 |
| GB2568895B (en) | 2017-11-29 | 2021-10-27 | Oxford Nanopore Tech Ltd | Microfluidic device |
| GB2569977A (en) | 2018-01-05 | 2019-07-10 | Oxford Nanopore Tech Ltd | Method |
| GB201801768D0 (en) | 2018-02-02 | 2018-03-21 | Oxford Nanopore Tech Ltd | Synthesis method |
| GB201807793D0 (en) | 2018-05-14 | 2018-06-27 | Oxford Nanopore Tech Ltd | Method |
| GB2574048B (en) | 2018-05-24 | 2021-06-16 | Oxford Nanopore Tech Ltd | Nanopore sensor component with electrostatic discharge protection |
| GB201808556D0 (en) | 2018-05-24 | 2018-07-11 | Oxford Nanopore Tech Ltd | Method |
| GB201808554D0 (en) | 2018-05-24 | 2018-07-11 | Oxford Nanopore Tech Ltd | Method |
| GB201809323D0 (en) | 2018-06-06 | 2018-07-25 | Oxford Nanopore Tech Ltd | Method |
| US20220162692A1 (en) | 2018-07-30 | 2022-05-26 | Oxford University Innovation Limited | Assemblies |
| GB201821155D0 (en) | 2018-12-21 | 2019-02-06 | Oxford Nanopore Tech Ltd | Method |
| JP7492200B2 (ja) | 2019-03-12 | 2024-05-29 | オックスフォード ナノポール テクノロジーズ ピーエルシー | ナノ細孔センシングデバイスと操作方法およびその成形方法 |
| GB201907244D0 (en) | 2019-05-22 | 2019-07-03 | Oxford Nanopore Tech Ltd | Method |
| GB201907246D0 (en) | 2019-05-22 | 2019-07-03 | Oxford Nanopore Tech Ltd | Method |
| WO2020254672A1 (en) | 2019-06-19 | 2020-12-24 | Therycell Gmbh | Spatial characterisation of target structures in a sample |
| GB201917060D0 (en) | 2019-11-22 | 2020-01-08 | Oxford Nanopore Tech Ltd | Method |
| CN114761799A (zh) | 2019-12-02 | 2022-07-15 | 牛津纳米孔科技公开有限公司 | 使用纳米孔表征靶多肽的方法 |
| GB201917742D0 (en) | 2019-12-04 | 2020-01-15 | Oxford Nanopore Tech Ltd | Method |
| GB202004944D0 (en) | 2020-04-03 | 2020-05-20 | King S College London | Method |
| US20230295712A1 (en) | 2020-06-18 | 2023-09-21 | Oxford Nanopore Technologies Plc | A method of selectively characterising a polynucleotide using a detector |
| GB202009349D0 (en) | 2020-06-18 | 2020-08-05 | Oxford Nanopore Tech Ltd | Method |
| JP2023530155A (ja) | 2020-06-18 | 2023-07-13 | オックスフォード ナノポール テクノロジーズ ピーエルシー | ナノポアを通って移動するポリヌクレオチドを特徴付ける方法 |
| EP4675256A3 (en) | 2020-07-17 | 2026-01-28 | Oxford Nanopore Technologies PLC | Nanopore sensing device |
| US12535457B2 (en) | 2020-07-22 | 2026-01-27 | Oxford Nanopore Technologies Plc | Solid state nanopore formation |
| ES2999861T3 (en) | 2020-08-11 | 2025-02-27 | Hoffmann La Roche | Nucleoside-5'-oligophosphates tagged with positively-charged polymers, nanopores incorporating negative charges, and methods and systems using the same |
| GB202015993D0 (en) | 2020-10-08 | 2020-11-25 | Oxford Nanopore Tech Ltd | Method |
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| GB202107192D0 (en) | 2021-05-19 | 2021-06-30 | Oxford Nanopore Tech Ltd | Method |
| GB202107354D0 (en) | 2021-05-24 | 2021-07-07 | Oxford Nanopore Tech Ltd | Method |
| WO2022263496A1 (en) | 2021-06-17 | 2022-12-22 | F. Hoffmann-La Roche Ag | Engineered nanopore with a negatively charged polymer threaded through the channel |
| GB202112235D0 (en) | 2021-08-26 | 2021-10-13 | Oxford Nanopore Tech Ltd | Nanopore |
| GB202118906D0 (en) | 2021-12-23 | 2022-02-09 | Oxford Nanopore Tech Ltd | Method |
| GB202118908D0 (en) | 2021-12-23 | 2022-02-09 | Oxford Nanopore Tech Ltd | Method |
| US20250066748A1 (en) * | 2021-12-31 | 2025-02-27 | Bgi Shenzhen | Helicase bch2x and use thereof |
| GB202205617D0 (en) | 2022-04-14 | 2022-06-01 | Oxford Nanopore Tech Plc | Novel modified protein pores and enzymes |
| WO2023215603A1 (en) | 2022-05-06 | 2023-11-09 | 10X Genomics, Inc. | Methods and compositions for in situ analysis of v(d)j sequences |
| JP2025517736A (ja) | 2022-05-17 | 2025-06-10 | オックスフォード ナノポール テクノロジーズ ピーエルシー | 方法及びアダプター |
| CN115074336B (zh) * | 2022-06-29 | 2024-03-22 | 电子科技大学 | 一种具有磷脂翻转酶活性的蛋白及其纯化方法 |
| GB202211607D0 (en) | 2022-08-09 | 2022-09-21 | Oxford Nanopore Tech Plc | Novel pore monomers and pores |
| GB202211602D0 (en) | 2022-08-09 | 2022-09-21 | Oxford Nanopore Tech Plc | Novel pore monomers and pores |
| KR20250044691A (ko) | 2022-08-09 | 2025-04-01 | 옥스포드 나노포어 테크놀로지즈 피엘씨 | 신규 포어 단량체 및 포어 |
| AU2023366350A1 (en) | 2022-10-28 | 2025-03-27 | Oxford Nanopore Technologies Plc | Pore monomers and pores |
| GB202216162D0 (en) | 2022-10-31 | 2022-12-14 | Oxford Nanopore Tech Plc | Method |
| GB202216905D0 (en) | 2022-11-11 | 2022-12-28 | Oxford Nanopore Tech Plc | Novel pore monomers and pores |
| WO2024138701A1 (zh) * | 2022-12-30 | 2024-07-04 | 深圳华大生命科学研究院 | 一种解旋酶突变体及其制备方法和在高通量测序中的应用 |
| WO2024138632A1 (zh) * | 2022-12-30 | 2024-07-04 | 深圳华大生命科学研究院 | 一种解旋酶、其制备方法及其在测序中的应用 |
| GB202304324D0 (en) | 2023-03-24 | 2023-05-10 | Oxford Nanopore Tech Plc | Method and kits |
| GB202305524D0 (en) | 2023-04-14 | 2023-05-31 | Oxford Nanopore Tech Plc | Novel pore monomers and pores |
| GB202307141D0 (en) | 2023-05-12 | 2023-06-28 | Oxford Nanopore Tech Plc | Modified helicases |
| GB202307486D0 (en) | 2023-05-18 | 2023-07-05 | Oxford Nanopore Tech Plc | Method |
| GB202315935D0 (en) | 2023-10-18 | 2023-11-29 | Oxford Nanopore Tech Ltd | Novel enzymes |
| GB202317028D0 (en) | 2023-11-06 | 2023-12-20 | Oxford Nanopore Tech Ltd | Method |
| WO2025120150A1 (en) | 2023-12-08 | 2025-06-12 | Oxford Nanopore Technologies Plc | Methods and apparatus for forming apertures, methods and apparatus for unblocking apertures, methods of sensing molecular entities in apertures, and measurement systems |
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| GB202407055D0 (en) | 2024-05-17 | 2024-07-03 | Oxford Nanopore Tech Plc | Enhanced polynucleotide sequencing |
| GB202407044D0 (en) | 2024-05-17 | 2024-07-03 | Oxford Nanopore Tech Plc | Polymer analysis using transformer neural network |
| GB202407100D0 (en) | 2024-05-17 | 2024-07-03 | Oxford Nanopore Tech Plc | A flow cell and a method |
| GB202407099D0 (en) | 2024-05-17 | 2024-07-03 | Oxford Nanopore Tech Plc | A flow cell and a method |
| GB202407247D0 (en) | 2024-05-21 | 2024-07-03 | Oxford Nanopore Tech Plc | Method |
| GB202407228D0 (en) | 2024-05-21 | 2024-07-03 | Oxford Nanopore Tech Plc | Method |
| WO2026052817A1 (en) | 2024-09-06 | 2026-03-12 | Oxford Nanopore Technologies Plc | Method |
| WO2026052811A2 (en) | 2024-09-06 | 2026-03-12 | Oxford Nanopore Technologies Plc | Method |
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| WO2026057755A1 (en) | 2024-09-13 | 2026-03-19 | Oxford Nanopore Technologies Plc | Adaptors and kits for rna molecules labelling and characterising |
| GB202413618D0 (en) | 2024-09-16 | 2024-10-30 | Oxford Nanopore Tech Plc | peptide characterisation |
| GB2644084A (en) | 2024-09-16 | 2026-03-18 | Oxford Nanopore Tech Plc | Augmented consensus and variant calling |
| GB202413747D0 (en) | 2024-09-18 | 2024-10-30 | Oxford Nanopore Tech Plc | Preprocessing nanopore signals |
Family Cites Families (48)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6602979B1 (en) | 1994-12-19 | 2003-08-05 | The United States Of America As Represented By The Department Of Health And Human Services | Screening assays for compounds that cause apoptosis |
| US6267872B1 (en) | 1998-11-06 | 2001-07-31 | The Regents Of The University Of California | Miniature support for thin films containing single channels or nanopores and methods for using same |
| KR20040023596A (ko) | 2001-05-01 | 2004-03-18 | 도꾸리쯔교세이호진 상교기쥬쯔 소고겡뀨죠 | 신규 맥시자임 |
| WO2002103210A1 (en) | 2001-06-15 | 2002-12-27 | Hansford Derek J | Nanopump devices and methods |
| US7399590B2 (en) | 2002-02-21 | 2008-07-15 | Asm Scientific, Inc. | Recombinase polymerase amplification |
| CA2498764C (en) | 2002-09-20 | 2015-11-10 | New England Biolabs, Inc. | Helicase dependent amplification of nucleic acids |
| US7745116B2 (en) | 2003-04-08 | 2010-06-29 | Pacific Biosciences Of California, Inc. | Composition and method for nucleic acid sequencing |
| US7851203B2 (en) | 2003-10-01 | 2010-12-14 | Lawrence Livermore National Security, Llc | Functionalized apertures for the detection of chemical and biological materials |
| US7238485B2 (en) * | 2004-03-23 | 2007-07-03 | President And Fellows Of Harvard College | Methods and apparatus for characterizing polynucleotides |
| WO2005124888A1 (en) | 2004-06-08 | 2005-12-29 | President And Fellows Of Harvard College | Suspended carbon nanotube field effect transistor |
| WO2006046455A1 (ja) | 2004-10-27 | 2006-05-04 | Kaneka Corporation | 新規カルボニル還元酵素、その遺伝子、およびその利用法 |
| GB0505971D0 (en) | 2005-03-23 | 2005-04-27 | Isis Innovation | Delivery of molecules to a lipid bilayer |
| GB0523282D0 (en) | 2005-11-15 | 2005-12-21 | Isis Innovation | Methods using pores |
| US20110121840A1 (en) | 2007-02-20 | 2011-05-26 | Gurdial Singh Sanghera | Lipid Bilayer Sensor System |
| EP2156179B1 (en) * | 2007-04-04 | 2021-08-18 | The Regents of The University of California | Methods for using a nanopore |
| WO2009035647A1 (en) | 2007-09-12 | 2009-03-19 | President And Fellows Of Harvard College | High-resolution molecular graphene sensor comprising an aperture in the graphene layer |
| GB2453377A (en) | 2007-10-05 | 2009-04-08 | Isis Innovation | Transmembrane protein pores and molecular adapters therefore. |
| GB0724736D0 (en) | 2007-12-19 | 2008-01-30 | Oxford Nanolabs Ltd | Formation of layers of amphiphilic molecules |
| US8231969B2 (en) | 2008-03-26 | 2012-07-31 | University Of Utah Research Foundation | Asymmetrically functionalized nanoparticles |
| EP2310534B1 (en) | 2008-07-07 | 2018-09-05 | Oxford Nanopore Technologies Limited | Base-detecting pore |
| US20110229877A1 (en) * | 2008-07-07 | 2011-09-22 | Oxford Nanopore Technologies Limited | Enzyme-pore constructs |
| US20100092960A1 (en) | 2008-07-25 | 2010-04-15 | Pacific Biosciences Of California, Inc. | Helicase-assisted sequencing with molecular beacons |
| US9080211B2 (en) | 2008-10-24 | 2015-07-14 | Epicentre Technologies Corporation | Transposon end compositions and methods for modifying nucleic acids |
| GB0820927D0 (en) * | 2008-11-14 | 2008-12-24 | Isis Innovation | Method |
| KR20110125226A (ko) | 2009-01-30 | 2011-11-18 | 옥스포드 나노포어 테크놀로지즈 리미티드 | 혼성화 링커 |
| EP2391732B1 (en) | 2009-01-30 | 2015-05-27 | Oxford Nanopore Technologies Limited | Methods using adaptors for nucleic acid constructs in transmembrane sequencing |
| AU2010215761B2 (en) | 2009-02-23 | 2017-04-06 | Cytomx Therapeutics, Inc | Proproteins and methods of use thereof |
| GB0905140D0 (en) * | 2009-03-25 | 2009-05-06 | Isis Innovation | Method |
| CN102405410B (zh) | 2009-04-20 | 2014-06-25 | 牛津楠路珀尔科技有限公司 | 脂质双层传感器阵列 |
| US9309557B2 (en) | 2010-12-17 | 2016-04-12 | Life Technologies Corporation | Nucleic acid amplification |
| US9127313B2 (en) | 2009-12-01 | 2015-09-08 | Oxford Nanopore Technologies Limited | Biochemical analysis instrument |
| WO2012033524A2 (en) | 2010-09-07 | 2012-03-15 | The Regents Of The University Of California | Control of dna movement in a nanopore at one nucleotide precision by a processive enzyme |
| KR101939420B1 (ko) | 2011-02-11 | 2019-01-16 | 옥스포드 나노포어 테크놀로지즈 리미티드 | 돌연변이체 세공 |
| EP4737389A2 (en) | 2011-05-27 | 2026-05-06 | Oxford Nanopore Technologies PLC | Coupling method |
| JP6298404B2 (ja) | 2011-07-25 | 2018-03-20 | オックスフォード ナノポール テクノロジーズ リミテッド | 膜貫通ポアを用いる二重鎖ポリヌクレオチド配列決定のためのヘアピンループ方法 |
| JP6457811B2 (ja) | 2011-09-23 | 2019-01-23 | オックスフォード ナノポール テクノロジーズ リミテッド | ポリマー単位を含むポリマーの解析 |
| CN104039979B (zh) | 2011-10-21 | 2016-08-24 | 牛津纳米孔技术公司 | 使用孔和Hel308解旋酶表征目标多核苷酸的酶方法 |
| US9617591B2 (en) | 2011-12-29 | 2017-04-11 | Oxford Nanopore Technologies Ltd. | Method for characterising a polynucleotide by using a XPD helicase |
| AU2012360244B2 (en) | 2011-12-29 | 2018-08-23 | Oxford Nanopore Technologies Limited | Enzyme method |
| KR102083695B1 (ko) | 2012-04-10 | 2020-03-02 | 옥스포드 나노포어 테크놀로지즈 리미티드 | 돌연변이체 리세닌 기공 |
| CA2879261C (en) | 2012-07-19 | 2022-12-06 | Oxford Nanopore Technologies Limited | Modified helicases |
| JP6429773B2 (ja) | 2012-07-19 | 2018-11-28 | オックスフォード ナノポール テクノロジーズ リミテッド | 酵素構築物 |
| US11155860B2 (en) | 2012-07-19 | 2021-10-26 | Oxford Nanopore Technologies Ltd. | SSB method |
| CA2901545C (en) | 2013-03-08 | 2019-10-08 | Oxford Nanopore Technologies Limited | Use of spacer elements in a nucleic acid to control movement of a helicase |
| US9644089B2 (en) | 2013-03-14 | 2017-05-09 | Arkema Inc. | Methods for crosslinking polymer compositions in the presence of atmospheric oxygen |
| GB201406151D0 (en) | 2014-04-04 | 2014-05-21 | Oxford Nanopore Tech Ltd | Method |
| CN117947149A (zh) | 2013-10-18 | 2024-04-30 | 牛津纳米孔科技公开有限公司 | 经修饰的酶 |
| GB201417712D0 (en) | 2014-10-07 | 2014-11-19 | Oxford Nanopore Tech Ltd | Method |
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