BR112014005105A2 - aumento do teor de lipídeos em microalgas através de manipulação genética de uma triacilglicerol (tag) lipase - Google Patents

aumento do teor de lipídeos em microalgas através de manipulação genética de uma triacilglicerol (tag) lipase

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Publication number
BR112014005105A2
BR112014005105A2 BR112014005105A BR112014005105A BR112014005105A2 BR 112014005105 A2 BR112014005105 A2 BR 112014005105A2 BR 112014005105 A BR112014005105 A BR 112014005105A BR 112014005105 A BR112014005105 A BR 112014005105A BR 112014005105 A2 BR112014005105 A2 BR 112014005105A2
Authority
BR
Brazil
Prior art keywords
microalgae
lipase
tag
lipid content
triacylglycerol
Prior art date
Application number
BR112014005105A
Other languages
English (en)
Inventor
Büchel Claudia
Barka Frederik
Bode Helge
Lorenzen Wolfram
Original Assignee
Johann Wolfgang Goethe Univ Frankfurt Am Main
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Johann Wolfgang Goethe Univ Frankfurt Am Main filed Critical Johann Wolfgang Goethe Univ Frankfurt Am Main
Publication of BR112014005105A2 publication Critical patent/BR112014005105A2/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C12N15/1137—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/12—Unicellular algae; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
    • C11B1/00—Production of fats or fatty oils from raw materials
    • C11B1/10—Production of fats or fatty oils from raw materials by extracting
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8247—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Virology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Nutrition Science (AREA)
  • Botany (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Fats And Perfumes (AREA)

Abstract

abstract the present invention relates to a method of increasing the lipid content in an organism, in particular a micro algae by modulating the activity of a triacylglycerol (tag) lipase. the invention also concerns organisms produced by this method, or microalgae, with increased lipid content, as well as their use in industry and medicine; in particular to obtain omega-3 unsaturated fatty acids. the invention relates furthermore to a nucleic acid from phaeodactylum tricornutum, which encodes a new tag lipase, and its use in influencing the proportion of fatty acids, in particular the content of polyunsaturated fatty acids in biomass. tradução do resumo resumo patente de invenção: "aumento do teor de lipídeos em microalgas através de manipulação genética de uma triacilglicerol (tag) lipase". a presente invenção se refere a um processo para aumentar o teor de lipídeos em um organismo, em particular, de uma microalga por meio de modulação da atividade de uma triacilglicerol (tag) lipase. a invenção se refere, além disso, aos organismos obtidos por esse processo, respectivamente, microalgas, com aumento do teor de lipídeos, bem como suas utilizações na indústria e na medicina; em particular, para a obtenção de ácidos graxos ômega-3 insaturados. a invenção se ocupa, além disso, com um ácido nucleico de phaeodactylum tricornutum, que codifica uma nova tag lipase, bem como sua utilização na influência da porção de ácidos graxos, em particular, do teor de ácidos graxos poli-insaturados em biomassas.
BR112014005105A 2011-09-06 2012-09-06 aumento do teor de lipídeos em microalgas através de manipulação genética de uma triacilglicerol (tag) lipase BR112014005105A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE102011113283A DE102011113283A1 (de) 2011-09-06 2011-09-06 Erhöhung des Lipidgehaltes in Mikroalgen durch genetische Manipulation einer Triacylglycerin (TAG)-Lipase
PCT/EP2012/067432 WO2013034648A1 (de) 2011-09-06 2012-09-06 Erhöhung des lipidgehalts in mikroalgen durch genetische manipulation einer tria-cylglycerin (tag)-lipase

Publications (1)

Publication Number Publication Date
BR112014005105A2 true BR112014005105A2 (pt) 2017-07-04

Family

ID=46785445

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112014005105A BR112014005105A2 (pt) 2011-09-06 2012-09-06 aumento do teor de lipídeos em microalgas através de manipulação genética de uma triacilglicerol (tag) lipase

Country Status (6)

Country Link
US (1) US20140256927A1 (pt)
EP (1) EP2753685A1 (pt)
JP (1) JP2014527803A (pt)
BR (1) BR112014005105A2 (pt)
DE (1) DE102011113283A1 (pt)
WO (1) WO2013034648A1 (pt)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2014207043A1 (en) * 2013-06-25 2014-12-31 Cellectis Modified diatoms for biofuel production
JP6362162B2 (ja) * 2013-07-05 2018-07-25 国立大学法人東京工業大学 油脂の製造方法
JP6568061B2 (ja) * 2013-07-19 2019-08-28 フェルメンタル 改変藻類株及び同株を用いるトリアシルグリセロール蓄積の方法
JP6627336B2 (ja) * 2015-09-02 2020-01-08 株式会社デンソー 真核微細藻類のリパーゼ突然変異体
WO2017129777A1 (en) 2016-01-29 2017-08-03 Total Raffinage Chimie Increased triacylglycerol production in microalgae
EP3986911A1 (en) 2019-06-20 2022-04-27 TotalEnergies SE Mutation of an acyl-coa synthase for increased triacylglycerol production in microalgae

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1075527A2 (en) * 1998-04-30 2001-02-14 E.I. Du Pont De Nemours And Company Triacylglycerol lipases
DE10026845A1 (de) 2000-05-31 2001-12-06 Ipk Inst Fuer Pflanzengenetik Triacylglycerol-Lipasen
MX281182B (es) 2001-05-14 2010-11-22 Martek Biosciences Boulder Corp Produccion y uso de una fraccion rica en lipidos polares, que contienen acidos grasos altamente insaturados omega-3 y/u omega-6, procedentes de microbios, de semillas de plantas y de organismos marinos geneticamente modificados.
US20080124756A1 (en) * 2004-09-03 2008-05-29 Solazyme, Inc. Light utilization alteration of photosynthetic microorganisms
BRPI0611060A2 (pt) * 2005-06-10 2016-11-16 Univ York célula isolada, célula de planta, planta transgênica, semente, método para fabricar um polipeptídeo de fosfolipase e/ou triacilglicerol lipase, uso de um polipeptídeo, métoo de triagem para a identificação de um agente com a capacidade de inibidor crescimento /pi viabilidade de planta vaso de reação método para aumentar o teor de ácido graxos de uma semente e, planta ou semente
WO2009125401A2 (en) * 2008-04-10 2009-10-15 Rosetta Genomics Ltd. Compositions and methods for enhancing oil content in plants
EP2390341B1 (en) * 2010-05-25 2018-06-27 Neste Oyj Process and microorganisms for production of lipids
PL2465868T3 (pl) * 2010-12-17 2016-08-31 Neste Oyj Ulepszenie produkcji lipidów

Also Published As

Publication number Publication date
JP2014527803A (ja) 2014-10-23
WO2013034648A1 (de) 2013-03-14
EP2753685A1 (de) 2014-07-16
DE102011113283A1 (de) 2013-03-07
US20140256927A1 (en) 2014-09-11

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Legal Events

Date Code Title Description
B08F Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette]
B08K Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette]