BR112013033692A2 - métodos e composições para segregar ácido nucleico alvo a partir de amostras de ácido nucleico mista - Google Patents

métodos e composições para segregar ácido nucleico alvo a partir de amostras de ácido nucleico mista

Info

Publication number
BR112013033692A2
BR112013033692A2 BR112013033692A BR112013033692A BR112013033692A2 BR 112013033692 A2 BR112013033692 A2 BR 112013033692A2 BR 112013033692 A BR112013033692 A BR 112013033692A BR 112013033692 A BR112013033692 A BR 112013033692A BR 112013033692 A2 BR112013033692 A2 BR 112013033692A2
Authority
BR
Brazil
Prior art keywords
nucleic acid
compositions
methods
mixed
target nucleic
Prior art date
Application number
BR112013033692A
Other languages
English (en)
Inventor
Roger Allyn Forsyth
Original Assignee
Flir Systems
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Flir Systems filed Critical Flir Systems
Publication of BR112013033692A2 publication Critical patent/BR112013033692A2/pt

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C12Q1/06—Quantitative determination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/43—Enzymes; Proenzymes; Derivatives thereof
    • A61K38/46—Hydrolases (3)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/43—Enzymes; Proenzymes; Derivatives thereof
    • A61K38/46—Hydrolases (3)
    • A61K38/465—Hydrolases (3) acting on ester bonds (3.1), e.g. lipases, ribonucleases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/30—Phosphoric diester hydrolysing, i.e. nuclease
    • C12Q2521/301—Endonuclease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/125—Specific component of sample, medium or buffer

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Toxicology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
BR112013033692A 2011-06-27 2012-06-26 métodos e composições para segregar ácido nucleico alvo a partir de amostras de ácido nucleico mista BR112013033692A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201161501569P 2011-06-27 2011-06-27
PCT/US2012/044256 WO2013003376A2 (en) 2011-06-27 2012-06-26 Methods and compositions for segregating target nucleic acid from mixed nucleic acid samples

Publications (1)

Publication Number Publication Date
BR112013033692A2 true BR112013033692A2 (pt) 2017-01-24

Family

ID=47424771

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112013033692A BR112013033692A2 (pt) 2011-06-27 2012-06-26 métodos e composições para segregar ácido nucleico alvo a partir de amostras de ácido nucleico mista

Country Status (7)

Country Link
US (3) US8940296B2 (pt)
EP (1) EP2723904A4 (pt)
JP (1) JP2014520530A (pt)
CN (1) CN103958697B (pt)
BR (1) BR112013033692A2 (pt)
CA (1) CA2840528C (pt)
WO (1) WO2013003376A2 (pt)

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US8927218B2 (en) * 2011-06-27 2015-01-06 Flir Systems, Inc. Methods and compositions for segregating target nucleic acid from mixed nucleic acid samples
BR112013033692A2 (pt) 2011-06-27 2017-01-24 Flir Systems métodos e composições para segregar ácido nucleico alvo a partir de amostras de ácido nucleico mista
EP3425065B1 (en) 2011-12-13 2021-04-21 Oslo Universitetssykehus HF Methods and kits for detection of methylation status
US10704021B2 (en) 2012-03-15 2020-07-07 Flodesign Sonics, Inc. Acoustic perfusion devices
CA3140836A1 (en) 2012-11-21 2014-05-30 Courtagen Life Sciences Inc. A method of removing amplicons of a non-target nucleic acid having one or more methylated cytosines from a sample
US10563248B2 (en) 2012-11-30 2020-02-18 Cambridge Epigenetix Limited Oxidizing agent for modified nucleotides
US9487959B2 (en) 2013-04-09 2016-11-08 Clinton D. Bunch Device for leveling and aligning tiles and method for leveling and aligning tiles
US9725710B2 (en) 2014-01-08 2017-08-08 Flodesign Sonics, Inc. Acoustophoresis device with dual acoustophoretic chamber
WO2016169579A1 (en) * 2015-04-20 2016-10-27 Qiagen Gmbh Method, lysis solution and kit for selectively depleting animal nucleic acids in a sample
US11708572B2 (en) 2015-04-29 2023-07-25 Flodesign Sonics, Inc. Acoustic cell separation techniques and processes
US11377651B2 (en) 2016-10-19 2022-07-05 Flodesign Sonics, Inc. Cell therapy processes utilizing acoustophoresis
EP3355939A4 (en) 2015-09-30 2019-04-17 Trustees of Boston University MICROBIAL DEADMAN AND PASS CODE EMERGENCY STOP SWITCHES
US11214789B2 (en) 2016-05-03 2022-01-04 Flodesign Sonics, Inc. Concentration and washing of particles with acoustics
CN109661467A (zh) * 2016-09-12 2019-04-19 豪夫迈·罗氏有限公司 用于纯化双链核酸的方法和组合物
US10465187B2 (en) 2017-02-06 2019-11-05 Trustees Of Boston University Integrated system for programmable DNA methylation
CN114900773A (zh) 2017-12-14 2022-08-12 弗洛设计声能学公司 声泳系统及其操作方法、控制声换能器及声学系统的方法
WO2019182891A1 (en) 2018-03-19 2019-09-26 Illumina, Inc. Methods and compositions for selective cleavage of nucleic acids with recombinant nucleases
CN109321647A (zh) * 2018-10-26 2019-02-12 苏州森苗生物科技有限公司 标记组合物及羟甲基化核酸文库的构建方法
CN110124073A (zh) * 2019-05-21 2019-08-16 临沂大学 一种去除动物制品中残留病毒颗粒的方法
CA3187549A1 (en) 2020-07-30 2022-02-03 Cambridge Epigenetix Limited Compositions and methods for nucleic acid analysis
WO2023087290A1 (en) * 2021-11-20 2023-05-25 Agricultural Genomics Institute At Shenzhen, Chinese Academy Of Agricultural Sciences Methods for nucleic acid detection
GB202204912D0 (en) * 2022-04-04 2022-05-18 Genomic Labs Ltd DNA separation

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US6168918B1 (en) * 1996-01-31 2001-01-02 American Home Products Corp. Method of detecting foreign DNA integrated in eukaryotic chromosomes
WO1999040222A1 (en) * 1998-02-04 1999-08-12 Variagenics, Inc. Mismatch detection techniques
FI116851B (fi) * 2001-05-03 2006-03-15 Fit Biotech Oyj Plc Ilmentämisvektori, sen käyttöjä ja menetelmä sen valmistamiseksi sekä sitä sisältäviä tuotteita
EP1402071A4 (en) 2001-06-08 2005-12-14 Us Genomics Inc METHOD AND PRODUCTS FOR THE ANALYSIS OF NUCLEIC ACIDS BASED ON THE METHYLATION STATUS
US8067556B2 (en) 2001-07-26 2011-11-29 Agilent Technologies, Inc. Multi-site mutagenesis
US7309571B2 (en) * 2003-02-03 2007-12-18 Ge Healthcare Bio-Sciences Corp. Amplification of self-ligated, circularized cDNA for expression profiling
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BR112013033692A2 (pt) 2011-06-27 2017-01-24 Flir Systems métodos e composições para segregar ácido nucleico alvo a partir de amostras de ácido nucleico mista

Also Published As

Publication number Publication date
CN103958697A (zh) 2014-07-30
CN103958697B (zh) 2016-10-12
EP2723904A2 (en) 2014-04-30
WO2013003376A2 (en) 2013-01-03
CA2840528C (en) 2018-07-17
WO2013003376A3 (en) 2013-02-21
EP2723904A4 (en) 2015-03-04
US20130337460A1 (en) 2013-12-19
US20160272963A1 (en) 2016-09-22
JP2014520530A (ja) 2014-08-25
US9790486B2 (en) 2017-10-17
CA2840528A1 (en) 2013-01-03
US10190113B2 (en) 2019-01-29
US8940296B2 (en) 2015-01-27
US20180112206A1 (en) 2018-04-26

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B11A Dismissal acc. art.33 of ipl - examination not requested within 36 months of filing
B11Y Definitive dismissal acc. article 33 of ipl - extension of time limit for request of examination expired