BR112013031564A2 - de-glicosilação in vivo de proteínas recombinantes por co- expressão com pngase f. - Google Patents
de-glicosilação in vivo de proteínas recombinantes por co- expressão com pngase f.Info
- Publication number
- BR112013031564A2 BR112013031564A2 BR112013031564A BR112013031564A BR112013031564A2 BR 112013031564 A2 BR112013031564 A2 BR 112013031564A2 BR 112013031564 A BR112013031564 A BR 112013031564A BR 112013031564 A BR112013031564 A BR 112013031564A BR 112013031564 A2 BR112013031564 A2 BR 112013031564A2
- Authority
- BR
- Brazil
- Prior art keywords
- pngase
- glycosylation
- vivo
- expression
- recombinant proteins
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
- C12N9/80—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in linear amides (3.5.1)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/44—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from protozoa
- C07K14/445—Plasmodium
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201161494141P | 2011-06-07 | 2011-06-07 | |
| US61/494,141 | 2011-06-07 | ||
| US201161564427P | 2011-11-29 | 2011-11-29 | |
| US61/564,427 | 2011-11-29 | ||
| PCT/US2012/041340 WO2012170678A1 (en) | 2011-06-07 | 2012-06-07 | In vivo de-glycosylation of recombinant proteins by co-expression with pngase f |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| BR112013031564A2 true BR112013031564A2 (pt) | 2017-06-27 |
| BR112013031564B1 BR112013031564B1 (pt) | 2021-11-03 |
Family
ID=46397622
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BR112013031564-4A BR112013031564B1 (pt) | 2011-06-07 | 2012-06-07 | Métodos para geração e expressão de um polipeptídio e sistema de expressão |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US11673926B2 (pt) |
| EP (1) | EP2718444B1 (pt) |
| CN (1) | CN103906840B (pt) |
| BR (1) | BR112013031564B1 (pt) |
| CA (1) | CA2839932C (pt) |
| WO (1) | WO2012170678A1 (pt) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9689016B2 (en) * | 2013-12-18 | 2017-06-27 | Caliber Biotherapeutics, Llc | Method for in vivo production of deglycosylated recombinant proteins used as substrate for downstream protein glycoremodeling |
| CN104974994B (zh) * | 2014-04-02 | 2019-02-26 | 复旦大学 | 一种用于n-糖蛋白去糖基化的糖苷酶及其应用 |
| WO2015161201A1 (en) * | 2014-04-18 | 2015-10-22 | University Of Georgia Research Foundation, Inc. | Carbohydrate-binding protein |
| EP3221455B1 (en) * | 2014-11-20 | 2020-08-12 | Yissum Research and Development Company of the Hebrew University of Jerusalem Ltd. | Compositions and methods for producing polypeptides with a modified glycosylation pattern in plant cells |
| CA3005304C (en) | 2015-11-13 | 2024-02-20 | Tarlan MAMMEDOV | Production of in vivo n-deglycosylated recombinant proteins by co-expression with endo h |
| CN105602923B (zh) * | 2016-01-18 | 2019-03-12 | 南京农业大学 | 一种n-糖酰胺酶d的异源表达及其应用 |
| CA3061176A1 (en) | 2017-04-24 | 2018-11-01 | University Of Georgia Research Foundation, Inc. | Sialic acid binding polypeptide |
| WO2020013774A1 (en) | 2018-07-12 | 2020-01-16 | Akdeniz Universitesi | In vivo post-translational processing of target proteins by furin in plants: engineering, expression and production of functional active human furin in n. benthamiana plants |
| WO2022115083A1 (en) * | 2020-11-24 | 2022-06-02 | Akdeniz Universitesi | Engineering, production and characterization of plant produced, soluble human angiotensin converting enzyme-2 as a therapeutic target in covid-19 |
Family Cites Families (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5703055A (en) | 1989-03-21 | 1997-12-30 | Wisconsin Alumni Research Foundation | Generation of antibodies through lipid mediated DNA delivery |
| US5238821A (en) * | 1989-05-16 | 1993-08-24 | Genzyme Corporation | Endo F-free PNGase |
| PH30997A (en) * | 1990-03-12 | 1997-12-23 | Ciba Geigy | Antipathologenically effective compositions comprising lytic peptides and hydrolytic enzymes. |
| US5693506A (en) | 1993-11-16 | 1997-12-02 | The Regents Of The University Of California | Process for protein production in plants |
| EP0772619B2 (en) | 1994-07-15 | 2010-12-08 | The University of Iowa Research Foundation | Immunomodulatory oligonucleotides |
| EP0865499B1 (en) * | 1995-09-14 | 2009-03-18 | Virginia Tech Intellectual Properties, Inc. | Production of lysosomal enzymes in plant-based expression systems |
| US6165718A (en) | 1996-01-10 | 2000-12-26 | Novo Nordisk A/S Novo Alle | Method for in vivo production of a mutant library in cells |
| WO2000000624A1 (en) * | 1998-06-26 | 2000-01-06 | Croptech Development Corporation | Production of urokinase in plant-based expression systems |
| IL142041A0 (en) | 1998-10-07 | 2002-03-10 | Syngenta Participations Ag | Therapeutically active proteins in plants |
| US20020174453A1 (en) * | 2001-04-18 | 2002-11-21 | Henry Daniell | Production of antibodies in transgenic plastids |
| EP1485492B1 (en) * | 2002-03-19 | 2011-12-21 | Stichting Dienst Landbouwkundig Onderzoek | Gntiii (udp-n-acetylglucosamine:beta-d mannoside beta(1,4)-n-acetylglucosaminyltransferase iii) expression in plants |
| US20040092470A1 (en) | 2002-06-18 | 2004-05-13 | Leonard Sherry A. | Dry powder oligonucleotide formualtion, preparation and its uses |
| CN1641037A (zh) * | 2004-10-13 | 2005-07-20 | 山东大学 | 利用n－糖苷酶f制备蛋黄寡糖的方法 |
| WO2007040568A2 (en) * | 2004-12-01 | 2007-04-12 | Arizona Board Of Regents, Acting For And On Behalf Of Arizona State Univeristy | Production and use of human butyrylcholinesterase |
| CN1746302A (zh) * | 2005-07-18 | 2006-03-15 | 山东大学 | 利用酵母生产非n－糖基化蛋白的方法 |
| CN1793324A (zh) * | 2005-11-25 | 2006-06-28 | 山东大学 | 利用酵母表达、生产及糖基化改造人干扰素－β的方法 |
| HUE031698T2 (en) * | 2007-06-15 | 2017-07-28 | Medicago Inc | Modification of glycoprotein production in plants |
| BR112013008377A2 (pt) * | 2010-10-08 | 2020-10-06 | Fraunhofer Usa Inc. | molécula de ácido nucléico isolada, para produzir dois ou mais polipeptídeos alvos em uma célula de planta, método para produzir dois ou mais polipeptídeos alvos em uma célula de planta, composição, método de tratamento de um indivíduo em necessidade de pelo menos um dentre os dois ou mais polipeptídeos alvos produzidos pelo método, método de tratamento de um indivíduo em necessidade da proteína produzida pelo método e método para induzir uma resposta imunológica protetora contra um patógeno em um indivíduo |
-
2012
- 2012-06-07 CN CN201280037381.XA patent/CN103906840B/zh active Active
- 2012-06-07 CA CA2839932A patent/CA2839932C/en active Active
- 2012-06-07 WO PCT/US2012/041340 patent/WO2012170678A1/en active Application Filing
- 2012-06-07 US US14/124,109 patent/US11673926B2/en active Active
- 2012-06-07 EP EP12730713.0A patent/EP2718444B1/en active Active
- 2012-06-07 BR BR112013031564-4A patent/BR112013031564B1/pt active IP Right Grant
Also Published As
| Publication number | Publication date |
|---|---|
| EP2718444B1 (en) | 2017-12-27 |
| EP2718444A1 (en) | 2014-04-16 |
| BR112013031564B1 (pt) | 2021-11-03 |
| CA2839932A1 (en) | 2012-12-13 |
| CN103906840A (zh) | 2014-07-02 |
| US20140186886A1 (en) | 2014-07-03 |
| WO2012170678A1 (en) | 2012-12-13 |
| US11673926B2 (en) | 2023-06-13 |
| CN103906840B (zh) | 2018-01-30 |
| CA2839932C (en) | 2022-07-05 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| B06F | Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette] | ||
| B06T | Formal requirements before examination [chapter 6.20 patent gazette] | ||
| B07D | Technical examination (opinion) related to article 229 of industrial property law [chapter 7.4 patent gazette] |
Free format text: DE ACORDO COM O ARTIGO 229-C DA LEI NO 10196/2001, QUE MODIFICOU A LEI NO 9279/96, A CONCESSAO DA PATENTE ESTA CONDICIONADA A ANUENCIA PREVIA DA ANVISA. CONSIDERANDO A APROVACAO DOS TERMOS DO PARECER NO 337/PGF/EA/2010, BEM COMO A PORTARIA INTERMINISTERIAL NO 1065 DE 24/05/2012, ENCAMINHA-SE O PRESENTE PEDIDO PARA AS PROVIDENCIAS CABIVEIS. |
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| B07E | Notification of approval relating to section 229 industrial property law [chapter 7.5 patent gazette] | ||
| B25G | Requested change of headquarter approved |
Owner name: IBIO, INC. (US) |
|
| B08F | Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette] |
Free format text: REFERENTE A 9A ANUIDADE. |
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| B08G | Application fees: restoration [chapter 8.7 patent gazette] | ||
| B06A | Patent application procedure suspended [chapter 6.1 patent gazette] | ||
| B09A | Decision: intention to grant [chapter 9.1 patent gazette] | ||
| B16A | Patent or certificate of addition of invention granted [chapter 16.1 patent gazette] |
Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 07/06/2012, OBSERVADAS AS CONDICOES LEGAIS. |